15results about How to "Efficient induction" patented technology

The invention relates to the technical field of biotechnology, in particular to an artificial cultivation method of cordyceps sinensis sporocarp, which comprises the following steps: placing cordyceps sinensis in a liquid fermentation culture medium for fermentation to obtain cordyceps sinensis conidia liquid; the method comprises the following steps: injecting a cordyceps sinensis fungus conidium solution to the surface of a hepialus armoricanus larva body to infect the larva, placing the injected hepialus armoricanus larva in a culture medium simulating natural ecological conditions, and breeding in a first artificial climate box to obtain bombyx batryticatus; putting the bombyx batryticatus in a substrate simulating natural ecological conditions, covering the bombyx batryticatus with a layer of substrate, and culturing in a second artificial climate box to obtain the bombyx batryticatus overgrown with fungus coats; and planting the bombyx batryticatus overgrown with the fungus coats in soil, and culturing in a third artificial climate box to obtain cordyceps sinensis sporocarps. The cordyceps sinensis cultivated by the method is short in production period and low in heavy metal content, and can be produced on a large scale.

The invention relates to the technical field of cell culture, and particularly discloses a method for inducing somatic cells into pluripotent stem cells and the pluripotent stem cells. Comprising the following steps: sequentially carrying out induction culture on somatic cells by adopting a culture medium I, a culture medium II, a culture medium III and a culture medium IV to obtain the pluripotent stem cells, the I-IV culture medium adopts a DMEM high-glucose culture medium as a basic culture medium; each of the culture medium I and the culture medium II comprises the following components: a retinoic acid receptor stimulant, a glycogen kinase inhibitor, a TGF (Transforming Growth Factor) beta inhibitor, a selective inhibitor of cytoskeleton rearrangement related coiled-coil protein kinase, a receptor tyrosine kinase inhibitor, an SMO (Sequence Matrix Oxidase) stimulant, a Janus kinase 1 inhibitor, a Janus kinase 2 inhibitor and a Menin-MLL interaction inhibitor. The method for inducing the somatic cells into the pluripotent stem cells has the advantages of being low in cost, easy to operate, high in transformation efficiency and the like, and the human somatic cells can be efficiently and stably induced and recompiled into the pluripotent stem cells.

The invention relates to a rabies virus glycoprotein-based mRNA vaccine as well as a preparation method and application thereof. The mRNA vaccine comprises: (1) an immunogen mRNA for expressing rabies virus glycoprotein, wherein the rabies virus glycoprotein is G protein, IG protein, 3G protein, 3IG protein or 3IG [delta] GC protein; and (2) a vaccine vector, wherein the vaccine vector is liposome nano-particles which can be utilized by mRNA (messenger Ribonucleic Acid). The invention also provides a preparation method of the vaccine and application of the vaccine in prevention of rabies virus infection. According to the invention, G proteins and IG proteins of rabies virus strains CTN strain, SAD strain and CVS strain are taken as immunogen basis for the first time and are optimized and combined, and the rabies virus mRNA vaccine with immunogen being G protein, IG protein, 3G protein, 3IG protein or 3IG delta GC protein and vaccine vector being liposome nanoparticles is developed.

The invention discloses a method for inducing bacteria to enter a viable and non-culturable state in a host body and application of the method. According to the induction method, the respiratory tract environment of a rat is used as an induction condition to enable bacteria to enter a VBNC dormant state, and different from an induction mode of in-vitro environment pressure or an in-vitro cell system, the method successfully and effectively induces the bacteria to enter the VBNC state by using a complete biological individual, and is simple and convenient to operate and easy to prepare; the induction method can effectively realize induction of VBNC state bacteria in a host body, has very important significance for further research on induction of viable and non-culturable bacteria and subsequent health risk evaluation, and can be used for carrying out research on analysis of bacteria and host reactions under the condition of interaction of multiple innate immune pathways; the research on the drug resistance change and the like of the VBNC state bacteria induced in the host has important significance for understanding the drug resistance development mechanism of the bacteria in the environment in the host.

The invention discloses a method for artificially inducing triploid percobrachium glehniae, and belongs to the technical field of triploid fish breeding. In order to solve the problems that gonad development affects the growth and meat quality of percobrachium glehniae, reduces the commodity quality of percobrachium glehniae and the like, the invention determines the optimal starting time, the optimal hydrostatic pressure and treatment time, the optimal temperature and the optimal chemical conditions of triploid percobrachium glehniae induction in combination with the triploid proportion of percobrachium glehniae, so that the percobrachium glehniae is induced into the triploid percobrachium glehniae. The method for producing the triploid percobrachium glehniae fish through combined induction of hydrostatic pressure, temperature shock and chemicals is established. The triploid percobrachium glehniae induction method established by the invention has the advantages of clear operation process, strong practicability, high efficiency and the like, and the induction efficiency of the triploid percobrachium glehniae can reach up to 85%, so that the induction method provided by the invention is suitable for artificially and efficiently producing the triploid percobrachium glehniae and has a wide application prospect. And a new technical approach is opened up for polyploidy breeding of the percobrachium glehniae.

The invention discloses an efficient cultivation method for triploid mandarin fish and belongs to the technical field of mandarin fish breeding. In order to solve the problems that the growth and meat quality of the mandarin fish can be influenced by gonad development, the commodity quality of the mandarin fish is reduced and the like, the proper starting time, the optimal hydrostatic pressure, the optimal treatment time, the optimal temperature and the optimal chemical conditions for inducing the triploid mandarin fish are determined by combining the triploid proportion of the triploid mandarin fish; the cultivation method for producing the triploid mandarin fish through combined induction of hydrostatic pressure, temperature shock and chemicals is established. The triploid mandarin fish breeding method established by the invention is clear in operation process and high in practicability, triploid mandarin fish can be efficiently induced and generated, and the induction efficiency of the triploid mandarin fish can be up to 93%. The triploid mandarin fish breeding method provided by the invention opens up a new technical approach for mandarin fish polyploidy breeding.

In order to improve the defects of the existing in-vitro totipotent stem cell culture technology, the invention provides an application of a lysosome inhibitor in the aspects of in-vitro induction and culture of totipotent stem cells and promotion of entry of the totipotent stem cells and maintenance of a totipotent state. According to the invention, three known lysosome inhibitors are used to promote the in-vitro induced totipotent stem cells to maintain more, faster and longer totipotent states, and more operable space is provided for clinical totipotent stem cell application.

It is an object of the present invention to provide: a complex of an antigen that is not encapsulated in a nanogel, and a nanogel; and a vaccine preparation comprising the complex. Specifically, the present invention provides a complex of a nanogel and a vaccine antigen, in which the vaccine antigen is coated with the nanogel

The present invention relates to an mRNA vaccine based on rabies virus glycoprotein and its preparation method and application. The mRNA vaccine comprises: (1) an immunogenic mRNA for expressing rabies virus glycoprotein, wherein the rabies virus glycoprotein is G protein, IG protein, 3G protein, 3IG protein or 3IG ΔGC Protein; (2) vaccine carrier, the vaccine carrier is a liposome nanoparticle that can be used by mRNA. The present invention also provides a method for preparing the vaccine and its use in preventing rabies virus infection. The present invention is the first to use the G protein and IG protein of the rabies virus strains CTN, SAD and CVS as the basis of immunogens, optimize and combine them, and develop immunogens that are G protein, IG protein, 3G protein, 3IG protein or 3IG ΔGC Protein, rabies virus mRNA vaccine with liposome nanoparticles as the vaccine carrier.

The invention provides an induction method and an induction culture medium for inducing Helleborus thibetanus petioles to regenerate adventitious buds, and solves the technical problem of low efficiency of inducing sprouted adventitious buds of Helleborus thibetanus rhizomes or overground stems in the prior art. A method for inducing regenerated adventitious buds of helleborus thibetanus petioles comprises the steps that petiole explants are disinfected and sterilized and transplanted to a regenerated adventitious bud induction culture medium, and the regenerated adventitious bud induction culture medium comprises 6-benayl aminopurine, forchlorfenuron, zeatin, 6-furfuryl aminopurine, indolebutyric acid, multivitamin tablets, a coagulator, cane sugar and a nutrient culture medium. According to the method, the helleborus thibetanus petioles are used as explants, adventitious buds can be efficiently induced, a helleborus thibetanus plant regeneration technical system is established, rapid propagation, high regeneration efficiency and rich explant sources are realized, meanwhile, a mature, stable and efficient helleborus thibetanus regeneration system is established, and the method has important significance on transgenic breeding of helleborus thibetanus.

The present invention discloses a metal element lipid composition with an atypical crystalline structure, a preparation method thereof and a use thereof. The calcium metal element in the lipid composition of the present invention has a specific structural morphology, which can overcome the problem that free metal ions are difficult to directly enter cells, successfully deliver the metal element into cells, and overcome the deficiency that traditional calcium phosphate cannot activate IFNβ. Functional experiments confirm that it can significantly induce the production of IFNβ, thereby playing an important role in immune responses such as anti-viral infection and anti-tumor.

The present invention provides a culture medium, a culture method and an application thereof for inducing C2 type iNKT cells. The culture medium for inducing C2 type iNKT cells includes IL-2, IL-12, IL-15, IL-18, 7DW8-5, GM-CSF, IL-4, TNF-α, LPS, wogonin, hyperoside, autologous plasma and AIM-V basal culture medium within a limited range. The proportion of C2 type iNKT cells in CD3+T cells obtained by culturing with the culture medium can reach 32.0%, which can achieve efficient induction and effective amplification, provide guarantee for the research and clinical use of C2 type iNKT cells, and the corresponding C2 type iNKT cells can secrete a higher level of IFN-γ without target cell stimulation.

The present application relates to DNA-peptide combination vaccine, provide the combination preparation for inducing specific immune response to antigen peptide, the preparation includes (I) the antigen peptide, and (II) expression vector, the expression vector encodes the chimeric hepatitis B virus core antigen polypeptide of the antigen peptide insertion or addition, wherein, the antigen peptide is inserted into the region of the amino acid residue 74~87 or 130~138 of hepatitis B virus core antigen polypeptide, or is added to the N terminal or C terminal of hepatitis B virus core antigen polypeptide, and the antigen peptide of (I) and the expression vector of (II) are substantially simultaneously administered to the subject.

The invention relates to a duckweed spore formation induction method which comprises the following steps: pretreating duckweed plants to obtain duckweed to be cultured; spraying the to-be-cultured duckweed according to a preset period by adopting a gibberellin solution, and stopping spraying when the to-be-cultured duckweed forms spores, so as to obtain the cultured duckweed. The to-be-cultured duckweed is regularly sprayed with gibberellin, so that the duckweed can be effectively induced to form spores, and no adverse effect is caused to duckweed plants. By means of the method, the duckweed resource breed conservation quality can be effectively improved, and remarkable effects are brought to cultivation of excellent duckweed quality and reduction of breed conservation cost.

The invention relates to the technical field of automobile parts, and particularly discloses a front auxiliary frame structure, which has an X direction, a Y direction and a Z direction which are pairwise intersected, and is characterized by comprising a front cross beam, a rear cross beam and two longitudinal beams, the two longitudinal beams are oppositely arranged in the X direction, the front cross beam and the rear cross beam are respectively connected between the two longitudinal beams along the X direction, and the front cross beam and the rear cross beam are arranged in the Z direction. The front cross beam is further provided with an extending beam extending in the Y direction, the extending beam is provided with at least one first induction part, the longitudinal beam is provided with a first end face and a second end face, and the first end face and the second end face are each provided with at least one second induction part. The invention further provides a vehicle. According to the front auxiliary frame structure, the extension beams are arranged, the first induction parts are arranged on the extension beams, and the second induction parts are arranged on the two end faces of the longitudinal beams, so that effective guiding in the safety collision force transmission direction can be achieved, the safety collision performance of the front auxiliary frame structure is improved, and the impact absorption capacity is improved.