41 results about "Hepialus armoricanus" patented technology

The invention provides a method for increasing the yield of natural cordyceps sinensis. The method comprises the following steps of (1) selecting a base for increasing the yield of natural cordyceps sinensis; (2) placing a trapping lamp; (3) preparing trapping liquid; (4) screening and preparing culture medium of hepialidae hirsutella sinensis and paecilomyces hepialid; and (5) controlling yield increase conditions. According to the method, at the adult stage, a high pressure mercury vapor lamp is used for trapping, so the imagoes of hepialus armoricanus around the lamp fly to the base, and the egg-laying amount is increased; ethanol, vinegar and sugar solution are sprayed on meadows beneficial to larva growth, so the larvae gather together; and the hepialidae hirsutella sinensis and the liquefied paecilomyces hepialid are sprayed on the gathering area of larvae to raise the infection rate of the larvae of hepialus armoricanus, so the aim of increasing the yield of cordyceps sinensis is achieved and the industrialized production is realized.

The invention discloses a feeding substrate and an artificial feeding method for increasing a later-period survival rate of larvae of hepialus armoricanus which is a host of cordyceps sinensis. The feeding substrate is a mixed substrate comprising to-be-processed feeding soil and soil with hyphae of pleurotus eryngii. The to-be-processed feeding soil is mixed soil comprising highland humus soil from cordyceps sinensis producing areas and coarse sand, particles of pleurotus eryngii solid mother strains are cultured in highland humus soil from the cordyceps sinensis producing areas and then are smashed to form the soil with the hyphae of the pleurotus eryngii, and the highland humus soil for culturing the particles contains saw dust and coarse sand. The feeding substrate and the artificial feeding method have the advantages that the hyphae of the pleurotus eryngii is fixedly planted and grown in the soil of the larva feeding substrate, so that nutrition can be provided for the larvae via zero distance, pleurotus eryngii fungi are used as antagonistic bacteria for preventing diseases and contaminating microorganisms, growth and reproduction of pathogenic bacteria can be competitively inhibited, the diseases can be effectively reduced, and the feeding substrate and the artificial feeding method have obvious features, merits and important practical significance on artificial cultivation of the cordyceps sinensis.

The invention discloses a hepialus armoricanus egg screening device, which comprises a scattering box, falling passages, separation passages and egg receiving boxes, wherein the falling passages are communicated with the scattering box; the separation passages are communicated with the falling passages; the egg receiving boxes are arranged below the separation passages, and comprise a plurality of egg receiving cavities; egg separation ports are formed in the bottoms of the separation passages, and are communicated with the egg receiving cavities respectively; hepialus armoricanus eggs with different sizes are screened to different egg receiving cavities through the egg separation ports. When the screening device works, the hepialus armoricanus eggs with different sizes are placed in the scattering box, fall to the separation passages through the falling passages, and are screened to the egg receiving cavities corresponding to the corresponding egg separation ports through the egg separation ports below the separation passages; therefore, the hepialus armoricanus eggs with corresponding sizes can be screened only by regulating the sizes of the egg separation ports. According to the hepialus armoricanus egg screening device, the hepialus armoricanus eggs can be automatically screened; compared with a manual screening mode in the prior art, the automatic screening mode has the advantages that the working efficiency is improved, and the labor intensity of a worker is reduced.

The invention provides artificial feed for bat moth larvae and a preparation method thereof. The feed is prepared from the following raw materials in parts by weight: 180 to 220 parts of wheat bran, 230 to 260 parts of fish meal, 40 to 60 parts of corn flour, 60 to 90 parts of soybean meal, 100 to 140 parts of serpentgrass powder, 110 to 140 parts of yeast powder, 15 to 25 parts of agar, 4 to 6 parts of ethylparaben and 400 to 600 parts of sterile water. An insect source is hepialus menyuanicus or hepialus armoricanus, and the artificial feed is successfully prepared by virtue of the feeding practice of larvae in four years and by virtue of continuous preparation, feeding, formula improvement and experience summarizing.

The invention provides composite hazelnut wine brewed from hazelnut meal and a preparation technology of the composite hazelnut wine.A hepialus armoricanus oberthur digestive enzyme serves as a fermentation enzyme preparation to enable the raw materials of the hazelnut meal, sweet potatoes, blueberries and the like to be subjected to fermentation brewing, and the composite hazelnut wine which has the health-care effects of improving immunity, resisting cancers and the like is obtained.The adopted raw materials are pure natural traditional Chinese medicines or materials achieving homology of medicine and food, the synergistic effect of the raw materials is utilized, the functional hazelnut wine which can improve immunity, lower blood glucose and blood lipid, resist the cancers, achieve health care and delay senility is prepared, the product is safe and free of side effect, has the significant health-care effect, can be drunk for a long time and has a good market development prospect; particularly, the hepialus armoricanus oberthur digestive enzyme effectively decomposes protein, starch and fats in hazelnuts into small-molecule amino acids, saccharides, glycerin and fatty acid, next-step fermentation brewing is promoted, and a large number of substances beneficial to human bodies are generated through further decomposition; the composite hazelnut wine has the effects of improving the immunity, lowering the blood glucose and blood lipid, resisting the cancers and achieving health care.

The invention discloses application of L-proline in increasing blastospore quantity and mycelial biomass of Cordyceps sinensis. In order to solve the problem of how to increase mycelial biomass duringliquid fermentation of Cordyceps sinensis and the problem of how to acquire massive infection blastospores during artificial cultivation of entomogenous fungi with Cordyceps sinensis, L-proline is added to a Cordyceps sinensis medium to induce Cordyceps sinensis to efficiently produce blastospores; after Cordyceps sinensis spores are induced to the medium with the L-proline and cultured for 30 days, the quantity of blastospores is 1.49-5.26 times as high as that of a group with no addition; after 45 days of cultivation, the quantity of the blastospores is increased by 17-52.6 times; after 60days of cultivation, mycelial dry weight is increased by 15.67-66.44%. The blastospores for infecting Hepialus armoricanus so as to artificially cultivate entomogenous fungi with Cordyceps sinensis can be acquired more efficiently herein; the efficiency of acquiring mycelia by liquid fermentation of Cordyceps sinensis can be improved; artificial cultivation time of Cordyceps sinensis can be shortened; cultivation cost can be reduced.

The invention discloses a method for obtaining ophiocordyceps sinensis by infecting hirsutella sinensis with host insects of hepialus armoricanus oberthiir larvae. According to the method, the three key steps of the spore morphology (blastospore) of the hirsutella sinensis to be injected, an injection method (operation under an aseptic condition) and post-injection care (at a low temperature of 0-6 DEG C) are improved, so that the infected hepialus armoricanus oberthiir larvae can simultaneously achieve the effects of low death rate (less than 30%) and high infection rate (even 100% of funguscarrying rate) and the effect of shortening the infection ossification time (3-5 months), and the artificial ecological tended ophiocordyceps sinensis with the consistent morphology with wild ophiocordyceps sinensis is obtained.

The invention belongs to the field of biotechnology, and particularly relates to a method for artificially raising larvae of hepialus armoricanus oberthur. The method uses inorganic mineral vermiculite instead of meadow soil as a feeding substrate to artificially raise the larvae of the hepialus armoricanus oberthur, and the method has the advantages that pathogens cannot survive in the inorganicvermiculite, pathogen infection sources causing the devastating diseases of the host larva can be completely cut off, and the pathogens cannot infect the host larvae inhabiting the feeding substrate,thereby greatly increasing the survival rate of the larvae. The method can effectively prevent and cure diseases, greatly improve the feeding efficiency of the larvae of the hepialus armoricanus oberthur, and lay a solid foundation for providing larvae sources for large-scale artificial cultivation production of cordyceps sinensis.

The invention discloses a method for culturing hepialus armoricanus oberthur cordyceps sinensis, which comprises the following steps: (1) selecting primary high quality strain for breeding secondary liquid strain; (2) filling wheat and water in mass ratio of 4:3 into a container, sterilizing for 150 minutes at high pressure and high temperature, and then putting into a sterile room for illumination for 50 minutes to obtain a culture medium; (3) inoculating the secondary liquid strain on the culture medium in the container to obtain the strain carrying culture medium and sealing the container; (4) keeping the strain carrying culture medium at 20-22 DEG C for fermenting; (5) after cordyceps sinensis grows on the culture medium, opening the container for ventilating and supplying oxygen, keeping the concentration of carbon dioxide to be less than 0.5%, and keeping the temperature to be 16-20 DEG C; and (6) after the cordyceps sinensis grows for about 60 days, collecting to obtain the hepialus armoricanus oberthur cordyceps sinensis. The invention has simple production process, small investment, quick effect taking, high yield and low production cost, and the produced hepialus armoricanus oberthur cordyceps sinensis has high nutrient value.

The invention relates to the biotechnical field, in particular to liquid and a method for screening hepialus armoricanus eggs. The liquid for screening the hepialus armoricanus eggs comprises cane sugar and buffer liquid. A layering effect can be realized by the cane sugar for the hepialus armoricanus eggs which develop to different degrees; a pH (potential of hydrogen) value can be effectively regulated by the buffer liquid, and a hepialus armoricanus hatching rate further can be increased by the aid of salt concentration. The liquid and the method for screening the hepialus armoricanus eggs have the advantages that quality detection can be quickly carried out on the development degrees of the hepialus armoricanus eggs by the aid of cheap and easily available materials and the simple and convenient method, the experimental hepialus armoricanus eggs can be optimized without being destroyed, and accordingly a hatching rate of screened serviceable eggs and a survival rate of hatched hepialus armoricanus can be increased.

The invention provides a cordyceps sinensis host hepialus armoricanus larva generation cyclic breeding method. The method includes the steps of natural growing pupa excavating introduction, larva forming, copulation, spawning, incubation, larva breeding, pupa introduction, larva forming, copulation, spawning and larva breeding generation circulation. Soil of a larva pupa chamber is prepared from,by weight, 40% of hepialus armoricanus origin place frozen earth, 20% of vegetable garden soil, 26% of high mountain humus, 10% of decayed grass roots, 3% of lime and 1% of fungus medicine, the pH value of the soil ranges from 5.5 to 6, and the water content is 40%. According to the cordyceps sinensis host hepialus armoricanus larva generation cyclic breeding method, natural wild pupae and moths are adopted as a first generation of pupae and moths for generation cyclic breeding, following next generations can be cyclically transformed with artificially bred larvae, pupa or moths do not need tobe caught in fields, and people can directly breed larvae for achieving generation cyclic breeding. The fungus medicine for resisting viruses and killing bacteria, fungi and pests is added into the soil of a pupa chamber and the larva pupa chamber, so that harm caused by the viruses, the fungi and pollution is reduced, and the yield is increased.

The invention provides a DNA bar code composition for identifying the origin of cordyceps sinensis. The DNA bar code composition is a fragment of a CO1 gene representing different origins of cordycepssinensis. The invention provides the application of the DNA bar code composition and the amplification primer thereof in identifying the origin of cordyceps sinensis. The invention further provides amethod for identifying the origin of the cordyceps sinensis. Based on the characteristics that the migration capacity of cordyceps sinensis host bat Hepialus armoricanus larvae is weak and the distribution place is only limited to a high-altitude area in China, a set of simple, rapid and easy-to-operate method for identifying the cordyceps sinensis origin is established.

The invention provides an artificial breeding matrix for feeding lepidoptera hepialidae insect larvae, and a preparation method of the artificial breeding matrix. The artificial feeding matrix comprises the following components in parts by weight: 1-3 parts of coconut shell powder and 3-10 parts of garden soil, wherein the matrix humidity is maintained at 35-60%. According to the artificial breeding provided by the invention, the alpine meadow soil of the origin place of cordyceps sinensis is not adopted, so that great significance on the ecological environmental protection of the Qinghai-Tibet Plateau is achieved. Moreover, the raw materials of the artificial breeding matrix have low prices and convenient preparation, can be used for the breeding in the whole growth period of hepialus armoricanus larvae, and is not only applicable to the breeding of hepialus xiaojinensis, but also can be popularized to the breeding of other species of hepialus armoricanus larvae.

The invention discloses a hepialus armoricanus larva infection device and method. The device comprises an infection box, an insertion port A, an insertion port B, a partition plate, a partition net and a net cover; the infection box is a box body of a cuboid structure, and the upper end of the infection box is provided with an opening. One side face of the infection box is horizontally provided with the insertion port A and the insertion port B which are in a long strip shape, and the insertion port B is located above the insertion port A; the partition plate is of a rectangular flake-shaped thin-plate structure and can be inserted in the insertion port B; the partition net is a rectangular flake-shaped net sheet and can be inserted in the insertion port A; after the partition plate and the partition net are inserted in the insertion port A and the insertion port B, the infection box is divided into an upper space, a middle space and a lower space; the net cover is of a cuboid structure, wherein the lower end of the net cover is provided with an opening, and the net cover is composed of net sheets, and covers the opening of the upper end of the infection box.

The invention discloses a cordyceps sinensis buccal tablet capable of reducing blood fat and a preparation method of the cordyceps sinensis buccal tablet. The cordyceps sinensis buccal tablet is prepared from cordyceps sinensis, red yeast rice, radix salviae miltiorrhizae and fructus crataegi. Conidia generated by induction of hirsutella sinensis are evenly sprayed onto hepialus armoricanus larvaefirstly, the cordyceps sinensis is cultivated under the suitable condition and includes the same index components as wild cordyceps sinensis, the problems that cordyceps sinensis is in shortage and is difficult to collect and high in cost can be solved effectively, and the popularization effect on artificial large-scale cultivation and production of the cordyceps sinensis is realized; and by adopting a formula of the cordyceps sinensis buccal tablet, tabletting can be directly conducted without granulation, the technological steps are simple and easy to operate, the prepared buccal tablet issmooth and tidy in appearance and excellent in quality, the oral cavity acting time can be prolonged, no accessory is added in the prepared buccal tablet, the cordyceps sinensis buccal tablet has a good auxiliary curative effect on people suffering from diseases such as hyperlipidemia, hypertension, diabetes, cardiovascular disease and nephrosis, and the health-care and treatment effects of the cordyceps sinensis buccal tablet are more significant.

The invention provides an artificial formula fodder for hepialus armoricanus. The artificial formula fodder is prepared by mixing fodder raw powder with a traditional Chinese medicine additive, mixedvitamins and mixed inorganic salts, wherein the fodder raw powder is dry powder of serpentgrass, thick large-leaved gentian, potatoes and carrots; turmeric powder is served as the traditional Chinesemedicine additive. The turmeric is added into the artificial formula fodder for hepialus armoricanus provided by the invention, so that the survival rate of hepialus armoricanus breeding can be effectively increased.

The invention discloses a pill for regulating a gynecological disease caused by female endocrine dyscrasia and a preparation method thereof. The pill is prepared from the following raw materials in parts by weight: 90-110 parts of angelica sinensis, 120-140 parts of astragalus membranaceus, 100-120 parts of radix puerariae, 30-35 parts of honey, 6-13 parts of hepialus armoricanus quasi penicillium powder and 25-32 parts of soy isoflavone. Compared with the prior art, the pill disclosed by the invention has the benefits that the pill is made by adopting a pure traditional Chinese medicine technology, the volatilization of the medicine efficacies of all traditional Chinese medicines is ensured, no drug with toxic and side effects is contained, the medicine proportion follows an ancient prescription, and modern pharmacopoeia is met. The pill has the efficacies of replenishing blood, activating blood, regulating menstruation, stopping bleeding, moistening intestines and relaxing bowels and has the effect of treating breast lump, facial chloasma, menstrual disorder, climacteric syndrome, uterine fibroid, ovarian cyst, insomnia and constipation.

The invention discloses a disinfection treatment method for hepialus armoricanus eggs, and relates to the field of artificial cultivation. According to the method, ozone is used for auxiliary disinfection, the concentration of acid and alkali is further reduced, and hurt to operators is further reduced. According to the specific scheme, the disinfection treatment method comprises the following steps: S1, cleaning the hepialus armoricanus eggs with warm water; S2, removing water from the surfaces of the hepialus armoricanus eggs; S3, carrying out alkali leaching by using a sodium hydroxide solution with the mass fraction concentration of 0.2%-0.3%; S4, diluting the hydrogen peroxide silver ion disinfectant stock solution and water according to the ratio of 1:150-1:250, and soaking the hepialus armoricanus eggs for 15-25 minutes; S5, airing the hepialus armoricanus eggs again; and S6, before the hepialus armoricanus eggs are transferred to the ground, adopting ozone with the concentration of 15-25 mg/m<3> to disinfect the ground where the hepialus armoricanus eggs are hatched and provided with plant root systems for 15 minutes, and then transferring the hepialus armoricanus eggs to the underground to be hatched. According to the disinfection treatment method for the hepialus armoricanus eggs, the hepialus armoricanus eggs are disinfected through ozone, and the operation time is short.

The invention discloses a cordyceps sinensis strain collecting device and method. The device comprises a water collecting tank, a pipeline, water seepage pipes, a collecting box, an isolator, multiplegrowth holes and a sprayer head; the water collecting tank is located above the collecting box, and connected with the pipeline, and the pipeline leads to the upper position and the side face of thecollecting box separately; the collecting box is of a cuboid structure with a normally-opened top; the isolator is located in the middle inside the collecting box, and the growth holes running throughthe isolator are formed in the isolator, and make the bottom of the collecting box communicated with the space of the upper portion of the isolator; the sprayer head is connected to the end, leadingto the upper position of the collecting box, of the pipeline; the two water seepage pipes are connected to the end, leading to the side face of the collecting box, of the pipeline, and stretch into the lower position of the isolator in the collecting box. By means of the device, favorable conditions are created for cordyceps sinensis to infect larvae of hepialus armoricanus, so that more cordycepssinensis is cultured; meanwhile, through the arrangement of the isolator, the cordyceps sinensis is isolated from other infectious microbes, the success rate of infecting the larvae of hepialus armoricanus by cordyceps sinensis is increased, and the humidity of a medium is automatically controlled.

The invention relates to the field of cordyceps sinensis cultivation and discloses a field cordyceps sinensis cultivation method. The method is high in popularization performance and controllability.The method includes: 1) culturing field hepialus armoricanus pupae to achieve moths through eclosion, inducing mating of male and female moths, and after egg laying, hatching to obtain eggs for standby application; 2) collecting a strain of field cordyceps sinensis, and preparing into bacterial soil for standby application; 3) sequentially spreading mountain undergrowth soil, ginseng fruits, serpent grass and the bacterial soil in a culture pond, spreading a layer of mountain undergrowth soil at the topmost layer, and keeping for standby application; 4) uniformly scattering the eggs into the culture pond in the step 3), and controlling the internal temperature of the culture pond in a range of 10-20 DEG C from October every year; 5-7 months later, replenishing ginseng fruits and serpent grass into the culture pond, continuing cultivation for 18 months, harvesting after fruiting bodies of cordyceps sinensis grow above the soil surface by 3-5cm, removing surface soil, and performing airdrying to obtain a finished product of cordyceps sinensis.

The invention discloses a culture medium capable of improving chitinase activity of hirsutella sinensis mycelia. The culture medium comprises chitin. Chitinase plays a key role in degrading chitin when entomogenous fungi invade insect epidermis, and the enzyme activity of chitinase directly influences the efficacy of the entomogenous fungi infecting the insect epidermis. How to improve the enzymeactivity of hirsutella sinensis chitinase is a key step of ophiocordyceps sinensis formation. By improving the enzyme activity of hirsutella sinensis mycelia, the infection capacity of the hirsutellasinensis mycelia to hepialus armoricanus larvae can be effectively improved, and therefore a theoretical basis is laid for artificial cultivation of ophiocordyceps sinensis.