Feeding substrate and artificial feeding method for increasing later-period survival rate of larvae of hepialus armoricanus which is host of cordyceps sinensis

A technology for the host of Cordyceps sinensis and bat moth larvae is applied in the fields of botanical equipment and methods, application, gardening, etc., which can solve the problems such as failure to achieve very stable results, decline in the number of insect populations, and low survival rate. Reduces disease incidence, enhances consistency and synchrony

Inactive Publication Date: 2014-04-02
浙江天使生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, diseases caused by pathogenic bacteria and fungi often lead to large-scale infection and death of larvae, a sharp decline in the number of larvae, and low and unstable survival rates in the later stag...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of rearing substrate soil for rearing larvae

[0031] 1. Preparation for sterilization: Bake plateau humus and coarse sand at 60°C for 4 hours before use; wood chips containing 50% (mass) moisture are generally made of miscellaneous wood chips (miscellaneous wood chips refer to different tree species) Sawdust mixture formed) was sterilized by high-pressure steam at 121°C for 30 minutes before use; the operating space was sterilized by ultraviolet light for 30 minutes; breeding boxes, containers, work clothes, etc. were sterilized accordingly.

[0032] 2. Preparation of Pleurotus eryngii mycelium soil to be inoculated: In a 1L container, add coarse sand with a volume of 150mL-350mL, 50mL-80mL miscellaneous wood chips with a water content of 50% (mass), and then fill with plateau humus Fill a 1L container, mix evenly, add sterile water while mixing, and adjust the water content of the soil in the container to 45%-75% (by mass).

[0033] 3. Pleurotus eryngii h...

Embodiment 2

[0038] Effects of different substrates on rearing of late larvae without feed addition

[0039] 1. Putting in the matrix: In three rearing boxes with an open area of ​​0.2 square meters, put different equal amounts of different raising substrates: the control group 1 is put into the plateau humus, and the blank in Example 1 is put into it. The control matrix is ​​the control group 2, and the matrix soil of the band Pleurotus eryngii mycelia that is put into the embodiment 1 is the experimental group.

[0040] 2. Putting and rearing of 4th instar larvae: Collect 300 4th instar larvae, weigh them one by one, and calculate the average body weight. Three equal parts were put into three rearing boxes. Keep the ambient humidity at 65%-90%. The feeding temperature was kept at 16°C-20°C in the initial stage, and after 3-5 days, the temperature was adjusted to 7°C. The feeding cycle is 1 month.

[0041] 3. Investigation on the growth state of larvae: After raising for 1 month, coun...

Embodiment 3

[0046] Effects of different substrates on rearing of late larvae fed carrots

[0047] The bat moth larvae are reared artificially in areas other than the Cordyceps sinensis production area, and common vegetable plants such as carrots, sweet potatoes, and potatoes are often used as feed for the larvae. First, the long journey to dig wild ingredients from the place of origin is high, and the cost is high; second, the growth rate of larvae can be even faster if some vegetable plants are fed. However, vegetable plants are generally easy to deteriorate, and fresh ingredients need to be replaced frequently, which increases daily management links and labor costs, increases interference with larvae, and significantly reduces the survival rate. In this example, the feeding of carrots, a vegetable plant, is examined.

[0048] 1. Putting in substrate and feed: In four rearing boxes with an open area of ​​0.2 square meters, put the same amount of different substrates and the sam...

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Abstract

The invention discloses a feeding substrate and an artificial feeding method for increasing a later-period survival rate of larvae of hepialus armoricanus which is a host of cordyceps sinensis. The feeding substrate is a mixed substrate comprising to-be-processed feeding soil and soil with hyphae of pleurotus eryngii. The to-be-processed feeding soil is mixed soil comprising highland humus soil from cordyceps sinensis producing areas and coarse sand, particles of pleurotus eryngii solid mother strains are cultured in highland humus soil from the cordyceps sinensis producing areas and then are smashed to form the soil with the hyphae of the pleurotus eryngii, and the highland humus soil for culturing the particles contains saw dust and coarse sand. The feeding substrate and the artificial feeding method have the advantages that the hyphae of the pleurotus eryngii is fixedly planted and grown in the soil of the larva feeding substrate, so that nutrition can be provided for the larvae via zero distance, pleurotus eryngii fungi are used as antagonistic bacteria for preventing diseases and contaminating microorganisms, growth and reproduction of pathogenic bacteria can be competitively inhibited, the diseases can be effectively reduced, and the feeding substrate and the artificial feeding method have obvious features, merits and important practical significance on artificial cultivation of the cordyceps sinensis.

Description

[0001] technical field [0002] The invention relates to a feeding substrate and an artificial feeding method for bat moth larvae, hosts of Cordyceps sinensis, and belongs to the field of special breeding in agriculture. Background technique [0003] Cordyceps sinensis is a rare and valuable medicinal material produced in the Qinghai-Tibet Plateau. It is also known as the "Three Treasures of Traditional Chinese Medicine" together with ginseng and velvet antler. It is a complex of fungi and insect larval corpses. Cordyceps fungus parasitizes the larval form of Lepidoptera bat moth insects living in the humus of the plateau, absorbs the nutrition of the host, and until the larva dies and becomes rigid, a rod-shaped sub-seat grows from its head, that is, it forms a combination of larvae and sub-seat ( grass-like) form of Cordyceps sinensis. [0004] The artificial cultivation of Cordyceps sinensis is very difficult. my country began its cultivation research in the lat...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01K67/033
Inventor 郑勇军
Owner 浙江天使生物工程有限公司
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