Method for quick acquisition of conidia

A technology for conidia and spores, which is applied in the field of extremely rapid acquisition of conidia, can solve the problems of not pursuing the yield of conidia, and in-depth research on the temperature, humidity and nutrient conditions of the spore-producing environment without microcirculation, so as to avoid contamination or contamination. The effect of changes in bacterial species traits and short sporulation cycle

Active Publication Date: 2016-04-13
YICHANG SHANCHENGSHUIDU CORDYCEPS
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Problems solved by technology

It can be seen that the traditional microcirculation sporulation is aimed at cultivating a small amount of conidia as the identification of the anamorph of Cordyceps sinensis, and does not pursue the output of conidia, and has not done in-depth research on the environmental temperature, humidity and nutritional conditions of microcirculation sporulation. Research

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  • Method for quick acquisition of conidia

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Experimental program
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Effect test

Embodiment 1

[0026] Get freshly collected ascospores, dilute them with 2% glycerin aqueous solution to a concentration of 4500 / ml ascospores solution; smear the ascospores solution evenly on the body surface of 50 3rd instar healthy bat moth larvae with a pre-sterilized fine brush , the amount of smearing for each worm is 20 μ l, and the bat moth larvae after smearing are placed in clean petri dishes with a cover that are moistened with filter paper. The humidity of the filter paper is controlled within the range of full wetting, full but not overflowing. Check every day, and add water according to the moisture status of the filter paper. The culture was carried out in a clean area with a temperature of 14°C and a relative humidity of 65%, protected from light. Real-time monitoring of sporulation using a fluorescence microscope (see attached figure 1 shown), stop culturing at 24h, and use 0.003mol / lKH containing 0.1% Tween 80 2 PO 4 The buffer solution elutes the conidia, and the larva...

Embodiment 2

[0028] Get freshly collected ascospores and dilute them with 3% glycerol aqueous solution to a concentration of 5000 / ml ascospores solution; use a pre-sterilized fine brush to evenly smear the ascospores solution on the body surface of 50 4th instar healthy bat moth larvae , the amount of smearing for each worm is 20 μ l, and the bat moth larvae after smearing are placed in clean petri dishes with a cover that are moistened with filter paper. The humidity of the filter paper is controlled within the range of full wetting, full but not overflowing. Check every day, and add water according to the moisture status of the filter paper. The culture was carried out in a clean area with a temperature of 15°C and a relative humidity of 70%, protected from light. Use a fluorescence microscope to monitor the sporulation situation in real time, stop the cultivation at 54h, and use 0.003mol / lKH containing 0.1% Tween 80 2 PO 4 The buffer solution elutes the conidia, and the larvae are pu...

Embodiment 3

[0030] Get freshly collected ascospores and dilute them with 4% aqueous glycerin solution to form an ascospore solution with an ascospore concentration of 5500 / ml; use a pre-sterilized fine brush to evenly smear the ascospore solution on 50 5th instar healthy bat moth larvae On the body surface, the amount of smearing for each worm is 25 μl, and the smeared bat moth larvae are respectively placed in a clean petri dish with a cover that is moistened with filter paper. The humidity of the filter paper is controlled within the range of full wetting, full but not overflowing. Check every day, and add water according to the moisture status of the filter paper. The culture was carried out in a clean area with a temperature of 16°C and a relative humidity of 75%, protected from light. Use a fluorescence microscope to monitor the sporulation situation in real time, stop the cultivation at 48h, and use 0.003mol / lKH containing 0.1% Tween 80 2 PO 4 The buffer solution elutes the conid...

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Abstract

The invention relates to a method for quick acquisition of conidia. The method is an improved microcycle conidiation technique, Chinese clothing hair sporangiospore is adopted, hepialus armoricanus larva body surfaces, or hepialus armoricanus larva exuvia or hepialus armoricanus adult wings are taken as the carrier, conidia are generated through induction directly without conducting mycelial growth, and in this way, the culture period is short, cost is low, the technology is stable, the yield is high, and a stable conidium source is provided for successful infection of hepialus armoricanus larvae.

Description

technical field [0001] The invention belongs to the field of biological sciences, in particular to a method for obtaining conidia extremely quickly. Background technique [0002] Cordyceps sinensis [Ophiocordycepssinensis (Berk.) G.H. Sungetetal.] is a traditional precious Chinese medicinal material native to the Qinghai-Tibet Plateau in my country. It is a complex of the larvae and larvae of Cordyceps sinensis parasitizing on the larvae of the genus Batmothidae. In recent years, with the popularization of the nutritional and medicinal value of Cordyceps sinensis, coupled with the continuous improvement of people's living standards, the demand for Cordyceps sinensis has continued to increase, leading to intensified indiscriminate mining and excessive resource destruction, resulting in a sharp decline in Cordyceps sinensis resources Therefore, it is imperative to artificially cultivate Cordyceps sinensis. Due to the problems of low infection rate of larvae or low survival ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00
CPCC12N3/00
Inventor 李文佳吕延华李全平张宗耀
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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