158results about How to "Avoid germs" patented technology

The invention discloses a 3D three-dimensional cell culture system which is applicable to cell experiments and bio-engineering production. The system comprises a cell culture dish, sterile soft hoses, a peristaltic pump, an optical fiber sensor, a signal processing system, a temperature controller, a gas controller, a cell culture solution storage tank, a carbon dioxide storage tank, a computer as well as associated software and a light source. According to a working principle, such information as temperature, pH, cell density and the like in the cell culture dish is monitored by virtue of the optical fiber sensor in real time and the information is analyzed and processed by virtue of the computer, so that related components are controlled to precisely regulate corresponding culture conditions, and various indexes in a cell culture medium are kept balanced and stable. The system can automatically complete such complex experimental operations as cell observation, culture solution replacing, temperature measurement, pH value regulation and the like, manual labor can be replaced, so that manual cost is reduced; and meanwhile, the system, which precisely controls culture conditions, can guarantee the stability and uniformity of experimental factors.

The invention relates the method of extracting purification pellet phenolic acid B from Sage plant, especially extracting purification pellet phenolic acid B from salvia miltiorrhizae. The method comprises the following steps: treating the salvia miltiorrhizae in microwave field for 1-10 minutes, adding water, or disintegrating salvia miltiorrhizae to 10-60 mesh, and adding water, treating it in microwave field for 1- 20 minutes, centrifuging, removing deposition, then carrying out chromatography with polyamide, removing impurity with water and 20-50% alcohol, washing pellet phenolic acid B with 60-80% alcohol, decompressing, thickening, freezing, drying and getting pellet phenolic acid B whose purify is above 95%. The invention has the advantages of simple technology, low cost, high purify and stable quality.

A saline-alkali soil deep-improving method by chemical-biological technology includes steps of S1, improving chemical components of the saline-alkali soil by chemical measures, firstly, adding humic acid fertilizer into the saline-alkali soil, then adding chemically acidic fertilizer into the saline-alkali soil, applying physiologic acidic fertilizer, and finally adding mineral chemical fertilizer into the saline-alkali soil; S2, improving the saline-alkali soil by biological measures, firstly, planting suaeda salsa of euhalophyte on the saline-alkali soil to be treated, or planting scutellaria strigillosa hemsl when salt content of the saline-alkali soil ranges from 0.2% to 0.3%. The saline-alkali soil deep-improving method provides a feasible, economic and efficient scheme for improving the saline-alkali soil in coastal regions, improvement time is short, effect is good and no backflow is caused after improvement.

A processing method of raw material of cultivating black fungus by using corn cob, relates to a processing method of raw material of cultivating black fungus, solves the problem of low output caused by high production pollution rate in the present cultivation of black fungus by using corn cob as raw material. The method comprises the following steps: weighing 44-59.5wt% of saw dust, 30-40wt% of corn cob, 8-12wt% of wheat bran, 1.5-2.5 wt% of bean powder and 1-1.5wt% of lime; then crushing corn cob, adding water and lime with stirring uniformly, mixing with saw dust, wheat bran and bean powder, adjusting water content, then packaging, carrying out a normal pressure sterilization to complete the process. According to the invention, by adding lime in the pretreatment of corn cob, rancidity and contamination are prevented, by utilizing the normal pressure sterilization, production pollution is reduced effectively, and compared with the present method of cultivating black fungus, the yield of black fungus raises by 10%. The method is suitable for cultivating black fungus.

The invention provides a water-soluble plant essential oil feed additive and a preparation method thereof. The feed additive is prepared from the following raw materials in percentage by weight: 15-30% of cinnamyl aldehyde, 6-19% of thymol, 4-7% of eugenol, 6-14% of carvacrol, 1-3% of an emulsifier and 30-60% of solvent water, wherein the emulsifier is propylene glycol and/or polyoxyethylene 20 sorbitan monooleate. The preparation method comprises the steps of (1) mixing the cinnamyl aldehyde, the thymol, the eugenol and the carvacrol at the designed weight ratio; (2) mixing a plant essential oil semi-finished product and the emulsifier at the designed weight ratio; and (3) adding an emulsified plant essential oil semi-finished product to the solvent water and fully stirring to obtain the finished product. According to the water-soluble plant essential oil feed additive, the high-content plant essential oil product can be obtained, and meanwhile, the plant essential oil product can be completely dissolved into water, is added through a drinking water system in a farm, and is convenient to use. The preparation method is reliable in technology, simple in operation and low in cost.

The invention discloses a system and method for controlling a non-contact touch screen and relates to the technical field of touch screen control. The control system comprises a thermal induction module, a dynamic image recognition module, a dynamic image locking module, an image display module and a command conversion control module, wherein the command conversion control module is used for converting locked gesture images and actions into corresponding control commands to control actions of the touch screen. The control system is capable of realizing quick, accurate and non-contact control of the touch screen. As a result, the hand of a user can be effectively protected against bacteria and the safety and the stability of using the touch screen are improved.

The invention provides a method for extracting hemopoietic stem cells from placentas, and especially relates to a method for extracting hemopoietic stem cells from placentas in a lavage manner. In one embodiment of the invention, the method comprises the following steps: 1, collecting the placentas; 2, carrying out initial inspection on the placentas; 3, pre-disinfecting the placentas; 4, detecting the placentas and maternal blood; 5, lavaging hemopoietic stem cells of the placentas; and 6, concentrating and purifying the hemopoietic stem cells. The method has the advantages presented in the specification, and a flow cytometer detection result of the hemopoietic stem cells obtained through the method shows that the hemopoietic stem cells have a very high percentage of CD34 positive cells.

The invention provides a method for lavaging hematopoietic stem cells from a placenta. The method comprises the following six steps: collecting the placenta, initially detecting the placenta, disinfecting the placenta in advance, detecting the placenta and maternal blood, lavaging the placenta hematopoietic stem cells, and concentrating and purifying the hematopoietic stem cells. The method and devices used are simple and convenient to operate; the eluted hematopoietic stem cells are low in damage, large in amount, high in concentration and strong in activity, can be applied for autologous or xenogenous hematopoietic stem cell transplantation, and are helpful to repair damaged tissues.

The invention discloses a traditional Chinese foot caring solution which is prepared from the following components in percentage by weight: 1 to 30 percent of first moisturizer, 1 to 30 percent of second moisturizer, 0.01 to 50 percent of traditional Chinese medicinal powder, 0.01 to 30 percent of active matter penetration enhancer, 0.01 to 10 percent of thickening agent, 0.01 to 5 percent of preservative, and the balance of deionized water. A preparation method of the traditional Chinese foot caring solution comprises the following steps of a, mixing and uniformly stirring the thickening agent, the traditional Chinese medicinal powder and a half of first moisturizer, then adding the deionized water, and performing homogenizing treatment on a mixture for 1 to 10 minutes; performing temperature preservation on the mixture for 5 to 20 minutes and then cooling to be 65 to 75DEG C, adding the second moisturizer, and uniformly stirring to form a mixture A; b, uniformly mixing the active matter penetration enhancer and the remaining first moisturizer to form a mixture B; c, after the mixture is cooled to be 45 to 50DEG C, mixing the cooled mixture A with the mixture B, and stirring and uniformly mixing the mixture A and the mixture B to form a mixture C; d, after the mixture C is cooled to be 35 to 40DEG C, adding the preservative, and stirring and uniformly mixing to obtain the traditional Chinese foot caring solution. The preparation method is simple; the traditional Chinese foot caring solution has replenishing and moisturizing effects and a certain traditional Chinese medicinal health-care function.

A feed of special aquatic animals for perventing sexual prematurity and diseases is prepared from multiple nutrients, biologic additive, drugs and sexual prematurity inhibitor.

The invention discloses a method for extraction of colistin sulphate. The method comprises the following steps of 1, a fermentation broth preparation process comprising adjusting a pH value to 4.0 by sulfuric acid, 2, a ceramic membrane filtration process comprising filtering the fermentation broth by a ceramic membrane, collecting a filtrate, and adjusting a pH value of the filtrate to 4.5-5, 3, a continuous moving bed absorption-desorption process, wherein through the continuous moving bed absorption-desorption process, a discharged liquid is obtained, 4, a discharged liquid decoloration process, 5, a nanofiltration concentration process, wherein in the nanofiltration concentration process, nanofiltration membrane pore sizes are in a range of 2100-400Da molecular weight cut-off and 10-20 times concentration is realized, and 6 a spray drying process. The method has a total colistin sulphate yield of 86 to 91% and has good energy-saving and emission-reduction effects.

The invention relates to an acidified soil conditioner, which contains the following raw materials in parts by mass: 30-60 parts of shell powder, 12-20 parts of carboxymethyl cellulose, 8-15 parts of polymethyl methacrylate, 5-20 parts of calcium cyanamide, and 10-25 parts of nanosilicon dioxide solution. The shell powder is used as a soil structure conditioner, a certain amount of chemical components are added to regulate the pH value of the soil system and kill harmful bacteria in soil and maintain effective microbial community, so that the improvement of the soil structure, microbial environment and pH value can be achieved; in addition, the acidified soil conditioner can also provide essential calcium element and silicon element for soil, and can be effective for a long term after being applied once. The acidified soil conditioner not only achieves the acidified soil conditioning function, but also can improve the soil activity, increase trace elements, inhibit pathogenic bacteria of crops and improve the pH value of the acidified soil to achieve the effects of improving the soil microenvironment and improving the self restoration capability of soil, so that the yield and quality of crops can be remarkably improved.

The invention relates to a stereo multilayer and multipurpose bean curd stick making machine which comprises at least two layers of soybean milk tanks fixed to a rack. The bottom of each layer of soybean milk tanks is provided with a heating system, the tail ends of the layers of soybean milk tanks are correspondingly connected with conveying belts, each conveying belt comprises a front conveying belt inclination section and a rear conveying belt horizontal section, the conveying belt connected with the lower layer of soybean milk tanks is located on the outer side of the conveying belt connected with the upper layer of soybean milk tanks, the conveying belt on the innermost side is made from a gapless or gap airtight material, the conveying belt on the outer side is made from a breathable material provided with gaps, and a hot-air blower located above the conveying belt on the outmost side and used for pre-drying all layers of conveying belts is further fixed to the fixing rack. Large-scale processing of bean curd sticks can be performed through the bean curd stick making machine, and mixed production of upper-layer bean curd sticks and bottom-layer soybean oil skin can be also achieved.

The invention discloses a preservative for an ammonia nitrogen degrading microbial inoculum. The preservative comprises water, an inhibitor, an antioxidant, a cell protective agent and inorganic salt modifiers, wherein the inhibitor is nitrite, the cell protective agent is glycerin and the inorganic salt modifiers are dihydric phosphate and hydrophosphate. The invention also discloses a preserving method of the ammonia nitrogen degrading microbial inoculum, wherein the method adopts the preservative to preserve the microbial inoculum. Compared with other preserving methods in the prior art, the preservative and the preserving method are advantageous in that 1) the preservative is advantaged by wide sources of components, low prices and relatively simpler and more convenient preservation, easy operation, no secondary pollution, and the like, and 2) during preservation of the microbial inoculum, contamination is effectively prevented and viable bacterium cells of a nitrococcus microbial inoculum are free of harm, metabolic activity is stable after preservation, the need of applying the microbial inoculum for reinforcing nitration functions in sewage treatment systems can be met, and the preservative and the preserving method are high in application value.

The invention relates to a scattered garbage pick-up device; a main rod is connected with a cylinder through a fastening screw, an upper handle is fixedly arranged on one side of the cylinder, a lower handle which corresponds to the upper handle is arranged in an open slot, a telescopic spring is arranged at the upper part of a connecting rod, the bottom end of the connecting rod is movably connected with a connecting strip and an eagle paw arranged in a U-shaped box at the lower part of the main rod through rivets, and a tension spring is arranged in the eagle paw. The pick-up device can pick up cigarette butts, paper scraps, bigger plastic bags, pop cans and other scattered garbage in the regions which can not be swept and cleaned by a machine or a broom, such as green belts, lawns, flowers and the like, reduce the fatigue caused by bowing and bending knees while picking up by hand and prevent the pathogenic bacteria infection when the garbage is contacted with the hand, the overall structure is simple, the use is convenient and the production is easy.

The invention discloses a continuous gradient supplementation method and device applied to efficient expanding propagation of haematococcus pluvialis. The continuous gradient supplementation method comprises the steps of A, compounding different gradient supplementation culture mediums; B, performing activation and species expanding on the haematococcus pluvialis: transferring sterilized culture mediums into a conical flask on a superclean working table, and performing inoculation with haematococcus pluvialis algae species; C, performing inoculation of the haematococcus pluvialis into a fermenter: detecting the quantity of cells of algae liquid after species expanding, performing thorough cleaning with fresh sterilized culture mediums, performing resuspending, and performing inoculation into the fermenter; D, monitoring culturing parameters/performing pH control: monitoring the algae liquid through a main control unit; and E, performing directional adjustment of C/N ratio of the culture mediums. The device consists of the fermenter, an air inlet/outlet, a sample inlet/out, a supplementation opening, a pH/temperature/dissolved oxygen control unit and a continuous gradient supplementation unit. Through the adoption of the method, infection with bacteria in the transforming and culture process can be avoided, and the utilization efficiency of substrates is improved; produced cellsare used for subsequent astaxanthin induction, and the survival rate is high; and the yield of biomass in the stage is as high as 0.30g L<-1>d<-1>, and the yield of the astaxanthin in the stage is ashigh as 15.45mg L<-1>d<-1>.

The invention discloses a white goat breeding method in the field of animal husbandry breeding. The method comprises the following steps of sheep house construction, feed configuration, wherein feed is prepared from, by weight, 14-18 parts of corncob, 5-7 parts of mushroom residues, 6-9 parts of bran, 4-6 parts of bean pulp, 2-3 parts of mountain flour, 5-8 parts of soya residues and 10-15 parts of buckwheat, the raw materials are pulverized through a pulverizerand mixed, placed into boiling water to be boiled for 8-10 min, filtering is performed to obtain a mixture, 3-5 parts of vinegar withthe concentration being 4-5% is added into the mixture, then, uniform stirring is performed, standing is performed for 2-3 under the temperature of 45-50 DEG C, and 2-4 parts of salt is added for uniform stirring for use; fermentation liquid preparation; feed preparation, wherein the mixture and fermentation liquid are mixed and fermented for 4-5 d under the temperature of 60-70 DEG C, and then, acentrifugal dehydrator is used for dehydration, and a fermented material is obtained; feeding and management are performed; sheep pen disinfection. By adopting the white goat breeding method, nutrient substances can be supplied to white goat in time, and the meat quality of the white goat is greatly improved.