151 results about "Larva currens" patented technology

The invention provides a method of full-artificial culturing Cordyceps sinensis, including sampling, strain separating, host insect collecting, breeding and feeding, infecting and continuing feeding, on artificial condition, largely culturing Hirsutella sinensis; feeding host insects in an scale room at low altitude, completing the infection of Hirsutella sinensis to the host insects and sexuality of Cordyceps sinensis. By artificial culture, the host grubs are big and good-quality, and the success rate of infection is high, the infection time is short, the quality is stable and controllable, and the pesticide effect test fully indicates that the pesticide effect of the produced Cordyceps sinensis is equivalent to that of the natural Cordyceps sinensis and it can overcomes the disadvantages of long growing time of natural Cordyceps sinensis and low natural infection ratio, and implements the industrialized production of Cordyceps sinensis.

The invention relates to the technical field of screening of animal species, in particular to a screening method for nicotine-resistant housefly species. The screening method comprises the steps that specific materials of a non-applicable fresh tobacco leaf formula are utilized to trap wild houseflies to come around to lay eggs, larvas of the wild houseflies are cultivated to a mature state, and the housefly species resistant to nicotine are screened according to growth and development indexes of the larvas. According to the screening method, the specific materials of the trapping formula are utilized, and therefore the housefly species effectively resistant to the nicotine can be screened out.

The invention provides a strain material adopting cordyceps sinensis mycoderma instead of the hirsutella sinensis spore bacterium as the cordyceps sinensis host infection bacterium. The strain material used for infecting hosts during cordyceps sinensis host raising is the cordyceps sinensis mycoderma. The strain material has the following beneficial effects: a method has the advantage of lower preparation cost of the strain material; and more importantly, by using the cordyceps sinensis mycoderma as the strain material, the survival rates and infection rates of the cordyceps sinensis hosts are obviously improved compared with the survival rates and infection rates of the hirsutella sinensis spore bacterium inoculated liquid and the host larvae.

The invention relates to the field of insect feeding, in particular to an artificial propagation technology of scleroderma guani, and provides application of thyestillageblerifacldermann larva as a host to artificial propagation of scleroderma guani. The artificial breeding technology of the thyestillageblerifacldermann has become more mature, and the breeding capacity is large, so that large-scale breeding can be realized. The thyestillageblerifacldermann is used as an alternative host for the scleroderma guani, so that the parasitism is strong and the survival rate of the scleroderma guani is high.

The invention discloses a double-queen culture method of Chinese bees. The method comprises the steps of selecting a healthy and strong swarm for rearing a queen bee from a series of swarms in the period of May to June before the natural swarming, selecting on one-day larva from each of the rest bee boxes to be cultured for 9 days in a queen rearing box, locking an old queen in the rest bee boxes and 3 to 6 worker bees onto a boundary board of the bee box, respectively introducing the queen cells into respective bee box after the queen cell is grown, observing cell outing and mating situation of the new queen, wherein if both the cell outing and mating are normal, and no fight exists, the two queens are successfully cultured. By adopting the method, a one-year-old queen and a novel bred queen can be cultured in a same bee box, the fight is avoided, the Chinese bees can be reproduced rapidly, the yield of honey can be increased, and the problems that the economic benefit for culturing the Chinese bees is low and the Chinese bee honey is in shortage in the market can be solved; meanwhile, the Chinese bees can be used for pollinating crops and traditional Chinese herbs, so that the yield of the crops and traditional Chinese herbs can be increased.

The invention discloses a method for expressing recombinant canine Alpha interferon with silkworm. Silkworm baculovirus serves as a carrier and recombinant virus containing a canine Alpha interferon gene is structured. Silkworm serves as a host of the recombinant baculovirus, and the recombinant canine Alpha interferon containing biological activity is expressed. The method solves the defects that complicated renaturation operations are needed in expressing the canine Alpha interferon in colon bacillus, the plasmid transformants in yeast expression are unstable and the production cost of mammal culture cells is too high, most of the recombinant canine Alpha interferon expressed by silkworm larvae is secreted into haemolymph, which is very good for the quick extraction of protein. Experimental results show that the method for using the recombinant baculovirus to produce recombinant canine Alpha interferon with silkworm is feasible. The largely expression of the recombinant canine Alpha interferon opens up broad prospects for the application of the Alpha interferon to medical and curing fields.

The invention belongs to the technique field of producing multi-peptide drugs by gene engineering. It clones human EGF gene into the silkworm bacilliform virus (Bombyx mori Nuclear polyhedrosis Virus) transferring carrier pBacPAK8 and makes them carry through homologous reformation in the silkworm cells to obtain the reformed virus BmBacEGF with human-coat growing gene. Use the reformed virus to to inocualte the silkworm grub and chrysalis by acupuncture in order to make the human-coat growing gene efficient expressed. Then make the grub and chrysalis into oral drugs which has remarkable action on the digestible ulcer by examination on animal.

The invention discloses a method for evaluating toxicity of pesticides on bee larva by the aid of laboratory artificial-breeding bee larva. The method includes steps of preparation of the bee larva, laboratory-breeding of the bee larva, preparation of tested pesticides, adding of the tested pesticides and the like and finally observation of the process that the larva complete defecation, spinning, pupation and eclosion. Through laboratory-breeding of the bee larva, research on the toxicity of the pesticides on the bee larva can be directly carried out in a laboratory without dependence on swarms; with the method, survival rate in breeding of the bee larva is quite high, and growth situations are basically identical; the method is labor saving, efficient and capable of obtaining the bee larva in batch with the growth states basically identical in a short time and evaluating effect of the pesticides on the bee larva visually, efficiently and accurately; meanwhile, many in-vivo experiments with the bee larva as objects can be carried out, high practicability and expansibility are achieved, and better materials and methods are provided for the relevant research and practical work.

The invention provides fodder for laying hens. The fodder is prepared from, in parts by mass, 58-62 parts of corn, 22-26 parts of soybean meal, 6-10 parts of stone powder for fodder, 4-6 parts of 5% compound premix feed for the laying hens and 2-4 parts of Hermetia illucens larva powder. The Hermetia illucens larva powder replaces fish meal to be added to the fodder for the laying hens to inhibit viruses and parasites in the laying hens, improve the intestinal tract environment of the laying hens and reinforce absorption of other nutritional ingredients in the fodder by the aid of the antibacterial and immune functions of antibacterial peptides in the Hermetia illucens larva powder and strong antibacterial and sterilizing functions of lauric acid in the Hermetia illucens larva powder. Multiple nutritional ingredients contained in the Hermetia illucens larva powder are used to replace the fish meal in the prior art, so that the purposes of improving the disease resistance of the laying hens and increasing the egg laying rate are achieved, and the egg laying rate can be increased by 1%-2% as compared with that with the fodder for the laying hens in the prior art.

A method for quickly detecting insect resisting cotton of transgenosis includes budding up cottonseed of transgenosis insect vesisting cotton to be detected, mixing it with manual feed of cotton bollworm, placing mixture in breeding box and putting larva of cotton bollworm in box, determining out death rate of larva of cotton bollworm for judging whether it is insect resisting cotton of transgenosis or not, confirming that it is if said death rate is greater than 65% or confirming that it is not if said death rate is less than 40%.

The invention relates to a method for killing root-knot nematodes with ozone-containing water. The insecticidal principle belongs to biological oxidation decomposition reactions, wherein ozone oxidizes and decomposes the 2nd larvae of the root-knot nematodes and the glucose oxidase necessary for oxidizing glucose in ova, reacts with the 2nd larvae of the root-knot nematodes and the ova directly to oxidize and destroy cell walls, DNA and RNA thereof and decompose proteins, lipids and polysaccharide macromolecular polymers so that the substance metabolic growth and the multiplicative process of cells are destroyed; and the ozone infiltrates cell membrane tissues and invades into cell membranes to act on the lipopolysaccharide in adventitial lipoproteins so that the cells have permeability distortions to cause cellular dissolutions and deaths, and oxidizes and dissolves the dead 2nd larvae of the root-knot nematodes, intra-ovular genetic genes, parasitic strains, parasitic virus particles, bacterial viruses, mycoplasma and pyrogens (bacterial virus metabolites and endotoxin) for degeneration deaths.

This invention relates to one kind of sheep multi-thread you sickness specificity gene and the separation and the appraisal, the design directs the thing, with the RT-PCR method, the larva stage which the sheep multi-thread you get sick separates the new gene, its naming is 45m, its length is 698bp, codes 232 amino acids. This gene and the sheep belt tapeworm, the cow belt tapeworm's protection gene piece has the very high homology. The quantity of protein molecule expressed is approximately 6,300 road Er pause. Swells 45 milligrams to pET41b in the expression vector, the construction tallies reads the code frame's fusion gene, the expression quantity may reach 100-120mg/L . Uses in sheep's immunising experiment this protein, may arouse organism effectively the immune response, its antiserum may has the specificity response with the prosopon and the larva antigen total protein in 63KD place , It is thought that this gene has the possibility to become the candidate vaccine member which the multi-thread you get sick.

The invention discloses industrial cultivation equipment for insect larvae. The industrial cultivation equipment comprises a vertical bracket and a connecting film, wherein the vertical bracket is provided with a plurality of layers of shelves for paving movable cultivation films; a steering shaft and a driving rotary shaft are respectively arranged at two sides of the vertical bracket; one end of the connecting film is fixedly arranged on the driving rotary shaft; the other end rounds the steering shaft and can be connected with the cultivation films paved on the shelves through hinges; and a sealing cover which can be used for closing or opening the vertical bracket is also arranged outside the vertical bracket. According to the industrial cultivation equipment disclosed by the invention, the loading and unloading of a culture medium are realized by using the movable cultivation films and relevant matched machines, such as the driving rotary shaft and the steering shaft; the automatic separation and collection of the larvae and the culture medium are realized by using a closed hypoxia space, and thereby the culture mechanization of the insect larvae is realized, the cost of labor force is saved, the efficiency is improved and the industrialization of insect breeding is facilitated.

The invention relates to a method for culturing cordyceps sinensis by performing endogenous pressurization and artificial infection of hirsutella sinensis on hepialus larvae, belonging to the technical field of artificial culture of the cordyceps sinensis. The method comprises the steps of: mixing the following components in percentage by weight: 28-34% of bombyx mori pupae, 5-7% of potatoes, 0.008-0.01% of magnesium sulfate, 0.035-0.05% of potassium dihydrogen phosphate and the balance of water, boiling, filtering and sterilizing to prepare an aseptic infection stock solution; taking the hirsutella sinensis as an infection bacteria source, picking pure mycelia through inoculating needles, placing the pure mycelia into the aseptic infection stock solution according to the proportion that the hirsutella sinensis picked by 10-15 needles are placed into every 5ml of the aseptic infection stock solution, grinding and then preparing an infection source bacterial solution; and sucking the infection source bacterial solution through a syringe, inserting a needle head from the excretory opening of each hepialus larva to press 0.3-0.5ml of the infection source bacterial solution into the intestinal tract of the larva, then placing the larvae in the shade and culturing under the conditions that the temperature is 17-21 DEG C and the relative humidity is 78-85% for more than 15 days. The method disclosed by the invention has the advantages of infection rate of up to above 90 percent, short period, low cost and great application prospects for large-scale culture of the cordyceps sinensis.

The invention discloses a simply and efficient method for preventing and controlling peanut diseases and insect pests. The method comprises the following steps of (1) improving quality of sowing, applying underground pesticides; (2) mechanized ridging, sowing, laying drip irrigation belt and film mulching; (3) drip irrigating and applying pesticides during seedling stage with the purpose of preventing and controlling underground insect pests, peanut stem rot, seeding blight and aphids, and avoiding weed growth; (4) drip irrigating and applying pesticides during flowering stage with the purpose of preventing and controlling red spiders, beet armyworms, the peanut stem rot and the seeding blight; (5) drip irrigating and applying pesticides during full fruit stage with the purpose of preventing and controlling the beet armyworms and leaf spot disease, and at the same time controlling the growth of the plants. The method has the advantages that compared with the prior art, the method can effectively prevent and control underground insect pests and aphids; the method can reduce late leaf senescence, reduce the leaf spot disease index by 41.51%, reduce the rate of rotten fruit and bud fruit, and improve the full fruit rate by 10.8%.

The present invention features that recombinant baculovirus AcNPV-hIL12 is hemocele injected to infect craze noctuid larva and after 96 hr the craze noctuid larva is sterilized with 75% concentrationalcohol and its haemolymph is collected via pricking its head with sterilized needle and stored in sterilized tube. The haemolymph is then centrifuged at 100,000 g for 30 min and the supernatant is collected. Human interleukin-12mAb is coupled to Sepharose CL-4B, and hIL-12 is purified with hIL-12mAb Sepharose CL-4B immunoaffinity column. The method of the present invention is convenient, and theobtained interleukin-12 is suitable for treatment of tumor, and bacteria, virus and parasite infected diseases.

The invention discloses a method for measuring acute and chronic toxicity of a drug against varroa jacobsoni. The method comprises the following steps: gently spraying the to-be-measured liquid onto the surfaces of healthy bee larvae; transferring the bee larvae from the inside of a larva culture plate into a bee pupa culture cup with the liquid-infiltrated filter paper piece at the bottom; collecting the to-be-measured varroa jacobsoni; introducing a varroa jacobsoni into the bee pupa culture cups of each treatment group and control group, and sealing the cup rims of the bee pupa culture cups with ventilated transparent films to prevent the introduced varroa jacobsoni from escaping the bee pupa culture cups; putting the bee pupa culture cups into an incubator for culturing, wherein the temperature is 34.5 DEG C, and the relative humidity is 75%; counting the deaths of varroa jacobsoni 48h or 72h later, calculating the death rate and analyzing the acute toxicity of the drug against varroa jacobsoni; or continuously recording the survival conditions of the varroa jacobsoni every day, counting the survival time of each varroa jacobsoni, and analyzing the chronic toxicity of the drug against varroa jacobsoni. The method disclosed by the invention has the advantages of controllable experiment conditions, high survival rate of the varroa jacobsoni, long survival time and accurate and credible result.

The invention provides a pine wood nematode RNAi regulatory gene. The full-length sequence of the RNAi regulatory gene is shown in SEQ ID NO:1, and the cDNA full-length sequence of the RNAi regulatorygene is shown in SEQ ID NO:2. The invention also provides application of the pine wood nematode RNAi regulatory gene in preparing a pine wood nematode control bactericide. A high-efficiency RNAi regulatory gene segment is screened, a positive fungal transformant containing the gene segment is fed to pine wood nematodes to obtains the distinct RNAi phenotype, and the Bx-etr-1 gene expression of the pine wood nematodes is obviously down-regulated. The pine wood nematodes have abnormal embryonic development, the larval mortality is high, the motion trail is changed, the motion speed is low, andthe population quantity is decreased significantly.

The invention discloses a method for all day feeding thamnaconus modestus early larvas by using shellfish early larvas, comprising the following steps of: 1) after performing artificial insemination on the shellfishes, washing away redundant semens, and transferring the obtained shellfish fertilized ovum to the clean natural seawater, containing the fertilized ova in a storage case or a bucket of 20-50 liters, and then placing in a refrigerator or a fresh-preservation cabinet at 4 DEG C; and 2) feeding for times every day. The method in the invention is capable of preserving the fertilized ova of shellfishes such as oysters, scallops and the like at low temperature, reducing the development rate of the fertilized ova, and enabling the fertilized ova and the larvas to be in a development stage suitable for feeding the thamnaconus modestus larvas for a long time interval; and via the method of increasing the feeding times and shortening the feeding interval, the larvas in a young fishes breeding pond are capable of eating the shellfish larvas as much as possible; and the possibility of development of the shellfish larvas into D-shaped larvas is reduced so as to guarantee adequate nutrient supply for the thamnaconus modestus early larvas.