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Fast inspection of transgenic cotton against pests

A detection method, the technology of insect-resistant cotton, applied in measuring devices, medical formulas, instruments, etc., can solve the problems that restrict the detection and screening of transgenic insect-resistant cotton, increase difficulty, and misleading, and achieve low detection cost and good consistency of results , The effect of short detection cycle

Inactive Publication Date: 2006-08-16
崔金杰
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AI Technical Summary

Problems solved by technology

The test period of this detection method is more than 30 days, which cannot meet the requirements of rapid detection of transgenic insect-resistant cotton
[0007] Due to the lack of a really fast, simple, low-cost, accurate and effective method for the rapid detection of transgenic insect-resistant cotton in my country, it seriously restricts the detection and screening of genetically modified insect-resistant cotton, and at the same time increases the difficulty for agricultural law enforcement departments, resulting in mixed seed markets. Phenomena of misleading, misleading, and pit farmers occur from time to time, hindering the process of seed industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Take 24 seeds of cotton 1 and 2 to be tested and cotton 3 and 4 to be tested respectively, soak them in water at 25°C for 72 hours, and repeat three times for each variety;

[0034] (2) prepare artificial feed for cotton bollworm, and weigh it with an electronic balance of 1 / 10,000, put it in a 24-hole insect rearing box, 0.5g in every hole, and sterilize the insect rearing box with 10% sodium hypochlorite (NaClO) aqueous solution before the test;

[0035] (3) After 72 hours, the soaked cottonseeds were artificially dehulled, and the single cottonseed kernels were homogenized in a mortar (inner diameter 5 cm), respectively, and the homogenate was quickly transferred into the 24-hole insect culture box respectively. and stir evenly with the feed in the box;

[0036] (4) According to the ratio of 17.5g agar per 1000ml of distilled water, configure the water agar solution, when the temperature of the melted agar drops to about 40-50°C, take about 1ml of the water agar ...

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Abstract

A method for quickly detecting insect resisting cotton of transgenosis includes budding up cottonseed of transgenosis insect vesisting cotton to be detected, mixing it with manual feed of cotton bollworm, placing mixture in breeding box and putting larva of cotton bollworm in box, determining out death rate of larva of cotton bollworm for judging whether it is insect resisting cotton of transgenosis or not, confirming that it is if said death rate is greater than 65% or confirming that it is not if said death rate is less than 40%.

Description

technical field [0001] The invention relates to a method for detecting insect-resistant cotton, in particular to a method for rapidly detecting transgenic insect-resistant cotton with a single cotton seed. Background technique [0002] At present, there are mainly four methods for detecting transgenic insect-resistant cotton. [0003] PCR (polyymerase chains reaction) method. For the internal standard genes Sad1, CaMV35S promoter, nos terminator, and various types of cry1A insect-resistant genes commonly contained in existing transgenic insect-resistant cotton, design specific primers for these genes for PCR amplification to detect whether the cotton contains transgenes Element. This method is limited to laboratory research, and requires specialized detection instruments such as PCR amplification instruments, etc., and the detection cost is high, and it is difficult for general companies, breeding units or individuals to bear the high detection costs. [0004] Quantitativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00A61K49/00
Inventor 崔金杰雒珺瑜申春玲马艳白雪莉
Owner 崔金杰
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