Cordyceps sinensis host infection strain material and method

A technology of Cordyceps sinensis host and Cordyceps sinensis fungus, which is applied to the strain material and host infection field infected by Cordyceps sinensis host, can solve the problems of difficulty in isolation and purification of Trichoderma sinensis strains, low larval infection rate and survival rate, etc., and achieves infection rate and the effect of improved survival rate and low production cost

Active Publication Date: 2012-11-28
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Abstract
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AI Technical Summary

Problems solved by technology

But above-mentioned method exists the difficulty of separation and purification of Mortierella sin

Method used

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  • Cordyceps sinensis host infection strain material and method
  • Cordyceps sinensis host infection strain material and method
  • Cordyceps sinensis host infection strain material and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 is used for the preparation method of the strain material of Cordyceps sinensis host infection

[0014] Collect the grown Cordyceps sinensis, and the specific collection method is: pry up the topsoil around the Cordyceps sinensis, can not touch the sclerotium surface of the sclerotium of the Cordyceps sinensis; peel off the clod around the Cordyceps sinensis, obtain the sclerotia similar to the sclerotia of the sclerotium of the sclerotium by one layer of appearance Cordyceps sinensis wrapped in a shell formed by a mixture of film and soil; collecting the shell formed by the mixture of film and soil to obtain a skin of Cordyceps sinensis.

[0015] Ultrasonic melting of the Cordyceps fungus skin with water to obtain a suspension of the Cordyceps fungus skin, filtering the soil in the suspension to obtain the first aqueous solution; washing the filtered soil with water, filtering to obtain the second aqueous solution, and combining the first And the second aq...

Embodiment 2

[0016] The investigation of the infection method of embodiment 2 Cordyceps sinensis host 1

[0017] The body fluid of the bat moth larvae was obtained from the living body of the bat moth larva infected with Mortierella sinensis, and the body fluid was stuck on the inoculation needle and smeared (that is, inoculated) on the plate medium under sterile conditions. Place the plate culture medium coated with the body fluid of live larvae carrying bacteria in an incubator at 8-18°C for cultivation. After 3 days, observe once a day to eliminate miscellaneous bacteria in time. After 10 to 16 days, dark gray and then brownish yellow or even black Mortierella sinensis colonies are formed at the inoculation site of the plate medium. Transfer the isolated Mortierella sinensis from a flat medium plate to a row medium, and culture it at 18°C. The center of the inoculum is yellowish brown, the colony is hard, and there are sparse and flat filamentous hyphae. Forms conidia of the fungus Cor...

Embodiment 3

[0023] The investigation of the infection method of embodiment 3 Cordyceps sinensis host 2

[0024] Collect the larvae of Hepialus xiaojinensisTu etMa, 2400 larvae in 8 pieces of 1m 2 In a clean sterilized enamel dish, 300 worms per group, put some sterilized dry leaves to facilitate the activity and concealment of the worms, and spray some distilled water to maintain humidity. Feed them with sterile Polygonum viburnum, carrots, sweet potatoes, and vegetable leaves for 7 days to restore the growth of the worms. Clean the surface of the worms in the above 6 groups, and disinfect the surface with 3% hydrogen peroxide; select any 4 groups, use a micro-injection gun, and inject the spore suspension prepared in Example 2 into the worms through the micropores on the surface of the worms under a microscope. In the blood cavity of the body, as the control group II; select any 4 groups, use a micro-injection gun, and inject the bacteria material used for the host infection of Cordycep...

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Abstract

The invention provides a strain material adopting cordyceps sinensis mycoderma instead of the hirsutella sinensis spore bacterium as the cordyceps sinensis host infection bacterium. The strain material used for infecting hosts during cordyceps sinensis host raising is the cordyceps sinensis mycoderma. The strain material has the following beneficial effects: a method has the advantage of lower preparation cost of the strain material; and more importantly, by using the cordyceps sinensis mycoderma as the strain material, the survival rates and infection rates of the cordyceps sinensis hosts are obviously improved compared with the survival rates and infection rates of the hirsutella sinensis spore bacterium inoculated liquid and the host larvae.

Description

technical field [0001] The invention relates to a method for cultivating Cordyceps sinensis, in particular to a strain material for host infection of Cordyceps sinensis and a method for host infection. technical background [0002] Cordyceps sinensis (Berk.) Sacc., also known as Cordyceps, is a complex of insects and fungi formed in the larvae of the genus Cordyceps in the family Ergotaceae, and is a rare and precious Chinese medicinal material unique to my country. It has the functions of invigorating the lungs and kidneys, relieving cough and reducing phlegm, and mainly treating diseases such as cough, hemoptysis, impotence and nocturnal emission, physical weakness after illness, and chronic cough in the elderly. Cordyceps sinensis is mainly produced in Tibet, Qinghai, Yunnan, Sichuan, Gansu and other provinces in my country, in local alpine meadow areas at an altitude of 3500-5200m. Natural resources are very limited. In recent years, excessive mining has caused the dete...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01K67/033
Inventor 刘飞张德利
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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