327 results about "Cordyceps myrmecophila" patented technology

The invention discloses a culture medium to ferment and manufacture Cordyceps sinensis mycelium, which consists of bevel culture medium and fermentation culture medium, wherein different culture mediums are adopted in different stages to ensure plain growth of the Cordyceps sinensis and equal quality of fermented and natural Cordyceps sinensis, which modifies the product quality and improves the production with certain effect. The invention supports the character of nourishment with natural Cordyceps sinensis, which is fit for industrial manufacturing need.

The invention relates to a fermented product which is obtained by fermenting valuable medicinal edible fungi, especially aweto, and a preparation method thereof. Raw materials for preparing the active component of the product comprises 1-2 loops of aweto strains or aweto, sea buckthorn, wild peaches, mulberries, dark plums, oranges, etc. The preparation method comprises the following steps of: culture preparation; aweto ferment raw juice preparation; fruit ferment raw juice preparation; probiotics fermentation. The beneficial effect of the invention is that: functional components are accumulated by aweto fermentation so as to achieve the purposes of multi-efficacy of aweto of lung invigoration and kidney nourishment, immunoenhancement, antioxidation, radiation resistance, and the like.

The invention discloses a manufacturing method of Chinese caterpillar fungus, which is characterized by the following: establishing greenhouse booth moth culturing field according to culturing temperature and moisture; reducing the danger of natural enemy of moth to accelerate weak plant root of herb and bush; releasing certain quantity of moth fertilized ovum, larva, pupal or and imagine in the culturing field; elevating moth larva breeding density with 110-3500000 per hectareha; breeding the moth from one year to 6-8 years within 365-425d; forming sac fungus; collecting 530000-1570000 Chinese caterpillar fungus per hectareha.

The invention discloses a method for culturing cordyceps militaris bacterium. The method is as follows: finished bacterium powder is obtained after natural cordyceps militaris is subjected to separation and purification, culturing on a test tube slant, culturing of liquid first-grade seeds and second-grade seeds in oscillators, culturing of small-size seeds in a seed fermenter, concentration and spray-drying. The method for culturing cordyceps militaris bacterium disclosed by the invention has the beneficial effects that: the yield of cordyceps militaris can be increased and the price of cordyceps militaris can be reduced fundamentally; and the method plays a role in relieving pain of all peoples suffering from diseases and makes a wonder for traditional Chinese medicine treasure.

The invention relates to a cordyceps militaris fruit body culture medium, mainly comprising a starch substrate and inorganic salt containing phosphorus, potassium and magnesium ions. The cordyceps militaris fruit body culture medium is characterized by further comprising cordyceps fungi powder which is powder containing cordyceps militaris or powder containing cordyceps militaris mycelium. The invention also provides a preparation method of the cordyceps militaris fruit body culture medium. The raw material of zhao cordyceps fungi powder as the important components of the culture medium is cultured waste material, namely culture medium after the cordycepin is cultured or waste material of the fruit body, and therefore, the production cost is reduced. The cordyceps militaris fruit body cultured by using the culture medium, the grown fruit body has excellent quality, and the content of adenosine and cordycepin as effective components is obviously improved compared with the common culturemedium.

The invention provides a cordyceps sinensis mycelium fermentation production method. The method includes the steps of preparation of a special culture medium for cordyceps sinensis mycelium liquid state fermentation, optimization of a mycelium provenance, fermentation of a strain in the culture medium and the like, wherein optimization of the mycelium provenance includes the procedures that cordyceps sinensis strain mycelia obtained after more than ten times of artificial passage are inoculated to the artificial solid culture medium, superior fruit bodies are selected, and a preferred China hirsutella rhossiliensis strain is separated from the fruit bodies. The cordyceps sinensis mycelium fermentation production method has the advantages that through strain selection, the cordyceps sinensis strain with strain character advantages is obtained, the screening effect is good, the problem of strain character recession can be effectively solved, and meanwhile the method also can serve as a China hirsutella rhossiliensis strain passage preservation method.

The invention provides a method for producing cordyceps sinensis strain powder through liquid fermentation, relating to strain powder. The method comprises the following steps of: 1) strain slant culture; 2) rice spore culture; 3) fermentation culture including primary seeding tank culture and secondary fermentation culture; and 4) strain powder preparation. By adopting the method, the seed enlargement frequency can be reduced, the fermentation period is about 120-160 hours, the fermentation time can be effectively shortened, the fermentation tank utilization rate can be improved, the productivity can be increased, the production cost can be saved, the final thalli yield reaches 4-6%, the thalli dry weight reaches 40-60g / L and the requirement of industrial production can be met.

The invention belongs to the technical field of fungus culture, particularly to a method for culturing mycelia and sporocarps of aweto, which solves the problem that artificial aweto in the prior art can not be cultured in a large scale. The method comprises the following steps of: selecting cephalosporium acremonium and Isaria cicadae; preparing a cephalosporium acremonium liquid strain and an Isaria cicadae liquid strain; preparing a solid culture medium from corn grit, soybean flour, wheat grains, sorghum grains, silkworm chrysalis meal, whole chicken eggs, black bean coarse powder containing hulls, degreased linseed oil and a nutrient solution, wherein the nutrient solution comprises chicken blood, pine needles, carrots, black beans and cattle bone; evenly mixing, and inoculating; and culturing to obtain the sporocarps and the mycelia. The detection indicates that the sporocarps contain at least 0.02% of adenosine and at least 0.1% of cordycepin, and the mycelia contain at least 0.055% of adenosine; the finger-print test result indicates that the degree of similarity of the sporophores and the mycelia is greater than 97%; and acute toxicity experiments and long-term toxicity experiments indicate that the sporophores and the mycelia have no toxicity and can be used as substitutes of natural aweto.

The invention provides a new technological method for preparing and industrially culturing aweto strains. The method comprises the following steps of: using young silkworms of 2 years as a host of an aweto culture medium, preparing strains by separating the tissue of wild awetos, dying the young silkworms, providing biological conditions suitable for aweto growth, and culturing aweto products with the shapes of wild awetos and the similar contents of various food mass. The invention has simple and convenient operation process, high yield rate and low culture cost.

The invention relates to a method for producing a microbial strain, in particular to a method for producing a new variety of Hirsutella sinensis. In the invention, Hirsutella sinensis screened according to cell dynamic information acquired by rat macrophage culture is subjected to multistage culture and fermentation, then hyphae is filtered by a membrane with a molecular weight between 300 and 4,000 by a solid-liquid separation technique, the bacterial solution is dried, and thus, health-care food and medicines, which can improve an immune function, are manufactured. When the method is used, the instability of industrial Cordyceps sinensis strain is realized, digital standard control is applied in a whole process from strain screening to culture and fermentation to solid-liquid membrane treatment, and the standard production process of industrial Cordyceps sinensis is realized actually; and with an accurate screening process, high production speed, pure production process and low production cost, the method can be widely accepted by people.

The invention provides a comprehensive method of extracting various effective components from cordyceps militaris. The dry and crushed cordyceps militaris is extracted and filtered with organic solvent to get filtrate and leftover; the filtrate is condensed to recycling the solvent, and the condensed liquor after being saponified, extracted and dried can present aweto sterols; the leftover is extracted and filtered, and the sediment and supernatant are obtained after condensing, alcohol sinking and centrifugating the filtrate; cordycepin polysaccharide is obtained through operations such as dissolving the sediment into water and removing the albumen; the supernatant is processed by methods such as ion-exchange resin column and crystallization to obtain cordycepin. The method is material saving, with high purity of the extracts, low cost and simple operation, thus realizing the comprehensive extraction of various effective components from cordyceps militaris.

The invention provides a strain material adopting cordyceps sinensis mycoderma instead of the hirsutella sinensis spore bacterium as the cordyceps sinensis host infection bacterium. The strain material used for infecting hosts during cordyceps sinensis host raising is the cordyceps sinensis mycoderma. The strain material has the following beneficial effects: a method has the advantage of lower preparation cost of the strain material; and more importantly, by using the cordyceps sinensis mycoderma as the strain material, the survival rates and infection rates of the cordyceps sinensis hosts are obviously improved compared with the survival rates and infection rates of the hirsutella sinensis spore bacterium inoculated liquid and the host larvae.

The invention provides a cordyceps sinensis health wine which is prepared from the fermentation stock liquid of cordyceps sinensis mycelia, the extraction liquid of traditional Chinese medicines and 58-62 DEG original basic white spirit prepared from pure grains, wherein the fermentation stock liquid of cordyceps sinensis mycelia is prepared by performing sterile body filtration on cordyceps sinensis strains through the deep liquid fermentation technology; and the extraction liquid of traditional Chinese medicines is prepared by extracting dwarf lilyturf tubers, radix polygonati officinalis, pulp of dogwood fruits, cistanche and epimedium herbs. The invention also provides a preparation method of the cordyceps sinensis health wine. The fermentation stock liquid of cordyceps sinensis mycelia adopted in the formula is a whole fermentation liquid mixed with the stock liquid of fermented thallis by using the specific fermentation technology, cells are broken, and the fermentation stock liquid of cordyceps sinensis mycelia is concentrated and compounded with a plurality of traditional Chinese medicines to prepare. The cordyceps sinensis health wine not only can reserve the effective ingredients of natural cordyceps sinensis and play the medical and health-care actions, but also can control production, the yield is increased, the cost is reduced, and the cordyceps sinensis health wine is applicable to popularization.

The invention discloses a Chinese caterpillar fungus combination containing plants and fungal polysaccharide and a preparation method thereof; edible-medicinal fungi and raw materials of Chinese herbal medicines are aqueously extracted by microwaves, and are concentrated for future use. The ultrasonic assistant enzymolysis, the filtration and the mixing are performed on filter residues of the edible-medicinal fungi to form a liquid culture medium; after being sterilized, the liquid culture medium is inoculated in Chinese caterpillar fungus strains for fermenting and collected to obtain Chinese caterpillar fungus mycelium; filter residues of the Chinese herbal medicines are mixed to a solid culture medium; after being sterilized, the solid culture medium is inoculated in cordyceps militaris strains for fermenting and cultivating; the ultrasonic alcohol extraction, the microwave aqueous extraction and the concentration are performed on the obtained cordyceps militaris; the mycelium and all concentrated solutions are merged, homogenized and dried to prepare the Chinese caterpillar fungus combination. The combination contains a plurality of components of cordycepin, adenosine, plants and fungal polysaccharide, and has the advantages of balanced nutrition, nature, mellowness and convenience for eating. Meanwhile, the resource reuse is realized in the liquid and solid cultivation manners to improve the added value of products; the preparation method is a production technology having the advantages of environmental protection, convenience for preparation and significant economic and social benefits.

The invention belongs to the microorganism field, particularly relates to a corayceps militaris polysaccharide two-stage fermentation synthesis process; the process comprises the following steps: corayceps militaris strains are vaccinated on a potato slant culture medium, are cultured at the constant temperature and then are put in a conical flash containing a seed culture medium for shaking culture; then the corayceps militaris strains are put in a fermentation tank containing a fermentation medium, the fermentation process is as follows: the corayceps militaris is cultured at constant temperature for four to five days in ventilating and stirring condition, which is mainly used for providing a culture condition suitable for growing of corayceps militaris thallus so that the corayceps militaris grows rapidly; then the corayceps militaris is cultured for two to three days at the constant temperature in the condition of ventilation and slightly stirring, a culture solution is centrifuged, and ethanol in two times of volume of is added into an obtained liquid supernatant which is mixed and put up for the night at the temperature of 4 DEG C; and precipitates obtained through secondary centrifugation is dried so as to obtain the corayceps militaris. The process is reasonable in design and simple in operation, is suitable for large-scale industrial production and can solve the situation of drug source shortage and has obvious benefits.

The invention discloses a Chinese caterpillar fungus cultivation method and its special-purpose culture medium, wherein the formulation comprises composite nitrogen source 10.0-50.0g, potato 200g, monosaccharide or disaccharide 0-20.0g, composite vitamin source 0-5.0g, vegetable oil 0-3.0g, peptone 0-5.0g, and balancing water. The culture process consists of, at a starting pH of 4.8-7.2, inoculating Cordyceps sinensis bacterial into single-purpose Cordyceps sinensis culture medium by the amount of 5-20%, then carrying out oscillation culturing at 12-21 deg C and 100-150rpm under natural daylight or 24h dark condition.

A buccal lozenge for nourishing lung and kidney, improving immunity, detoxicating and relieving fatigue is prepared from cordyceps powder, American ginseng and flavouring filler through proportional mixing and tabletting. Its a advantage is high effect.

A method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers is used for inoculating bacterial suspension prepared by using wild natural cordyceps sinensis bacterial strains native to the Tibet plateau on live silkworm pupas and artificially culturing the live silkworm pupas into silkworm pupa cordyceps sinensis. The method comprises the following steps of (1) screening of cordyceps sinensis bacterial strains, (2) preparation of the silkworm pupas, (3) preparation of bacterial suspension, (4) preparation of silkworm pupas before inoculation, (5) inoculation, (6) culturing of seed seats, and (7) collection and storage of silkworm pupa cordyceps sinensis. According to the method, silkworm pupas of domesticated silkworms serve as hosts, the cordyceps sinensis bacterial strain suspension is inoculated in the silkworm pupas artificially, and then the silkworm pupas are cultured into the silkworm pupa cordyceps sinensis. Inoculation rate and petrification rate after inoculation are above 96%, and the yield of seed entities is above 92%. The period from inoculation to mature of the seed entities takes 36-44 days. The chemical composition of the silkworm pupa cordyceps sinensis is the same with that of cordyceps sinensis basically, and the active ingredients such as cordycepin and cordycepic acids of the silkworm pupa cordyceps sinensis are 3.2-3.9 times the active ingredients of the cordyceps sinensis.

The invention provides a method for isolating the asexual stage bacterial species of Mortierella sinensis from the body fluid of the living bat moth larvae infected with Mortierella sinensis; Mortierella sinensis, a live bat moth larva infected with Mortierella sinensis, can be used to isolate and cultivate the asexual stage strains of Cordyceps sinensis many times. Compared with the spore separation method and tissue separation method, the operation is simpler, the contamination rate of miscellaneous bacteria is low, and biological materials are saved. The bat moth larvae that have been extracted from the body fluid can also grow Cordyceps sinensis through continuous feeding. The recycling of biological propagation materials in the cultivation process of Cordyceps sinensis is realized.

The invention provides a brewage method of a Chinese caterpillar fungus health-care yellow rice wine. The yellow rice wine is brewed with a saccharification agent of Chinese caterpillar fungus rice produced with Chinese caterpillar fungus as a raw material. The brewage method comprises the steps such as rice soaking, elution and rinsing, steaming, rice paving, distiller's yeast culturing, jar fermentation, pressing, blending, pottery jar aging, and the like. Compared with other health-care yellow rice wine, cordycepin, adenosine, and cordyceps polysaccharide in the yellow rice wine obtained in the invention are metabolites of the saccharification and fermentation agent Chinese caterpillar fungus. Compared with existing Chinese caterpillar fungus health-care yellow rice wine, Chinese caterpillar fungus is adopted as the saccharification and fermentation agent in the invention. Therefore, in the respect of cost, the method provided by the invention is better than yellow rice wine fermentation methods in which Chinese caterpillar fungus fruiting body and Chinese caterpillar fungus mycelium are adopted as additives. According to existing patents, Chinese caterpillar fungus mycelium is commonly adopted as an additive. The Chinese caterpillar fungus mycelium has a main content of protein whose odor can easily deteriorate the taste of wine. With the method provided by the invention, the obtained yellow rice wine has good taste.

The invention discloses a method for improving vitamin D content in a cordyceps sinensis calcium strengthening agent by ultraviolet irradiation, which comprises the following steps of: a, preparing a culture medium: preparing a liquid culture medium from a carbon source, a nitrogen source, mineral elements and the like, and sterilizing the prepared culture medium; b, inoculating cordyceps sinensis fungus into the culture medium, and performing shake culture to prepare liquid cordyceps sinensis fungus; c, inoculating the cordyceps sinensis fungus into the culture medium, performing the shake culture under certain conditions, and taking fermentation liquor out; and d, selecting a UV-B ultraviolet light source with the power of 14 to 60 W and the wavelength of 280 to 325 nm, pouring the cordyceps sinensis fungus fermentation liquor into a large culture dish, placing the ultraviolet light source over the culture dish, irradiating the prepared fermentation liquor, concentrating and drying the fermentation liquor, and breaking wall and grinding the obtained product into a powdery product. The method has the advantages of simple process, convenient operation and wide application to prevention and treatment of rickets of children, acalcerosis of women and osteoporosis of old peoples.

The invention discloses a method for extracting melanin from cordyceps militeris fungus fermentation broth. The method includes the following steps: mycelium is separated from the cordyceps militeris fungus fermentation broth, and the fermentation broth is acidized to obtain melanin crude extract after the sediment is collected. The melanin crude extract is hydrolyzed by hydrochloric acid to eliminate protein impurities, and is repeatedly scrubbed by organic solvent and dissolved by alkaline solution, and is acidized to form sediment for purification, and then the chloride ions are eliminatedby deionized water through repeated scrubbing, finally the cordyceps militeris melanin end product is obtained after drying. The melanin prepared by the invention has similar properties with melanin CAS: 8049-97-6 sold by Sigma Corporation. Both two types of melanin can dissolve in alkaline solution, cannot dissolve in organic solvents, such as water, ethanol, acetone, chloroform and hexane, completely become sediment when pH value is less than 3 and can fade under the effect of strong oxidizer; the ultraviolet visible light area scan map includes a curvature with reducing absorbance along with the increasing of wavelength, and strong absorbing bands are respectively near 340cm-1 and 1602cm-1 in the infrared spectrum.

The invention relates to a bionic culture method for winter worm summer herb mycelium, which comprises: separating and pure culturing monospore, preparing liquid medium, inoculating the pure culture bacterium of mycelium, static culturing the superficial-layer liquid for six months; harvesting the mycelium; wherein, breaking the mycelium into monocellular or multicellular mycelium every month during the culture process. This invention needs no fermentor or other dear devices, and reduces cost more than 50% for 1g dry mycelium.

The invention provides a cordyceps militaris strain cultivation method. Cordyceps militaris can be used as medicine instead of cordyceps sinensis and can take the place of a natural cordyceps sinensis medicinal herb resource. According to the technical scheme, the method includes the following steps of (1) maternal strain preparation, (2) conversion from maternal strains to mother seeds and cultivated species, (3) preparation of suspension strains or liquid strains, and (4) artificial cultivation of cordyceps militaris strains.

The invention discloses a cordyceps sinensis mycelium beverage and a preparation method thereof. The beverage comprises the components in percentage by weight: 1 to 30 percent of cordyceps sinensis mycelium stock solution, 0.1 to 0.5 percent of edible acid, 0.02 to 0.06 percent of edible sweetener, 2 to 9 percent of sugar, 1 to 5 percent of honey and the balance of water. The preparation method comprises the steps of: inoculating cordyceps sinensis strains into culture solution in a fermenter for culture, and stopping culturing until the strains achieve the index of cordyceps sinensis contents to prepare the cordyceps sinensis mycelium stock solution; pulverizing the stock solution through a colloid mill or pulverizing mycelia by using a viscolizer; mixing the stock solution with the edible acid, the edible sweetener or the sugar or the honey and the balance of water; performing sterilization; and preparing a canned, bottled or cartoned beverage. The cordyceps sinensis mycelium beverage has the advantages of good activity, no need of decocting extraction, convenient drinking and low cost, is favorable for the absorption of human bodies, and has the effects of immunity improvement, fatigue resistance, aging resistance and oxygen-poor resistance.

The invention discloses a large-scale cultivation method for cordyceps militaris. The large-scale cultivation method comprises the following steps: performing separation and purification cultivation on wild cordyceps militaris serving as an original strain; inoculating a liquid cordyceps militaris strain to a healthy and undeveloped oak silkworm pupae body, and performing box-type cultivation or bottle-type cultivation to obtain the cordyceps militaris. According to the large-scale cultivation method for the cordyceps militaris, the technical problems of low rigidifying rate, low germination rate, unevenness and the like of the conventional artificial cultivation method for the cordyceps militaris are solved.

The invention provides a method for asexual propagation of wild aweto fungi and belongs to the technical field of biology. In the method, living cells of wild aweto are used as strains, and the aweto fungi suitable for artificial culture are obtained in a special potato dextrose agar (PDA) culture medium by a cloning technology; and the PDA culture medium comprises the following components by mass: 950 to 1,050 grams of water, 280 to 320 grams of potatoes, 4 to 8 grams of peptone, 3 to 5 grams of potassium dihydrogen phosphate, 1 to 2 grams of magnesium sulfate, 15 to 20 grams of agar and 18 to 23 grams of glucose, and the pH value is between 6.0 and 7.0. The method comprises the following steps: 1, selection of living cells; 2, domestication of strains; 3, purification; and 4, rejuvenation and obtaining of excellent mother seeds of aweto. The method has the advantages of reasonable nutrients of the PDA strain culture medium, high strain activity, no pollution by molds and bacteria, stable quality of fungi and no mutation of fungi. In addition, the content of effective nutrients of the aweto is high, and the good-quality and high-yield fruit body is obtained.

The invention relates to edible fungi food and a manufacture method thereof, in particular to an edible fungi food and the preparation method thereof. The raw material used by the invention is preciously edible and medicinal fungi ganoderma spore powder, aweto mycelia powder and fine cordyceps militaris powder, and wild termite mushroom, wild matsutake and wild golden mushroom, which are compounded with salvia miltiorrhiza, astragalus and Chinese medicinal material used for preparing polygonum with the function of health-caring and the action of cooperating. The preparation method can be seen in the specifications for detail. The edible fungi food of the invention has reasonable compounding by the edible fungi and the Chinese medicinal material which has health-caring function and can completely regulate the physiological function of body after being eaten; especially when people in sub-health state eat the edible fungi food, the non-normal physiological activation and the low physiological state can be recovered to a normal state, thus improving the health-recovery capability of the body and gradually recovering the health state. The preparation method of the invention is simple and reliable.

The invention discloses a method for improving vitamin D content in a lucid ganoderma calcium fortifier by ultraviolet irradiation. The method comprises the following steps of: a, preparing a culture medium, namely preparing a liquid culture medium from a carbon source, a nitrogen source, mineral elements and the like, and sterilizing and cooling the prepared culture medium; b, inoculating lucid ganoderma strains into the culture medium, and performing shaking culture under certain conditions so as to obtain liquid cordyceps sinensis strains; c, adding a certain amount of liquid lucid ganoderma strains into the culture medium, introducing gas, stirring for culture, and taking the fermentation liquor out; and d, selecting a UV-B ultraviolet light source with the power of 14 to 60W and the wavelength of 280 to 325nm, pouring the fermentation liquor of fungus lucid ganoderma into a large culture dish, putting the ultraviolet light source over the culture dish to irradiate the obtained fermentation liquor, concentrating and drying the irradiated fermentation liquor, and breaking walls and grinding into powdery products. The method for improving the vitamin D content has the advantages of simple process and convenient operation, and can be widely applied to the prevention and treatment of rickets in children, calcium deficiency in women and osteoporosis in old people.

The invention discloses a cordycepin extract and notoginseng extract and application in prevention and cure pulmonary fibrosis medicine preparation, which is characterized by the following: extracting and condensing from China clothing hairs pore zymocyte powder with water and alcohol; getting cordycepin extract; setting Cordyceps sinensis adenosine, cordycepic acid, and Cordyceps sinensis polysaccharide more than raw bacteria powder about 3-8 double; extracting cordycepin extract from cordycepin crude drug with alcohol; separating large aperture absorption resin; purifying; getting the cordycepin extract; setting the content of cordycepin saponin more than raw cordycepin crude drug for 7-10 double; allocating the cordycepin and cordycepin saponin with mass percent at 90-10% and 10-90%; preparing medicinal compound. This invention possesses very big utility value in clinic.