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Cordyceps militaris fruit body culture medium and preparation method thereof

A technology of Cordyceps militaris seed and culture medium, applied in the culture medium of biological bacteria, culture medium of Cordyceps militaris sporocarp and its preparation field, can solve unfavorable industrial large-scale cultivation, influence on product yield and quality, and low yield of wild Cordyceps sinensis and other issues to achieve the effect of reducing production risks and management costs, enhancing competitiveness, and reducing costs

Inactive Publication Date: 2010-03-10
浙江天使生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solved the defects of low yield of wild Cordyceps sinensis, high production cost, and low biotransformation rate
But this method cultivation cost is relatively high, if need to use pure water, silkworm powder etc., so be unfavorable for industrialized large-scale cultivation; The operability of this formula is not strong, as this formula material water ratio is 90: 50 That is 1:0.6) is very different from the conventional optimal material-water ratio of 1:1.4, and the water is seriously insufficient, which will seriously affect the product yield and quality; in addition, the anti-staining ability is not strong, and there is no antibacterial component in the medium , unable to provide protection for the growth of Cordyceps fruiting bodies and other defects

Method used

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  • Cordyceps militaris fruit body culture medium and preparation method thereof
  • Cordyceps militaris fruit body culture medium and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0021] The preparation method of Cordyceps fungus powder A (pure fungus): take harvested Cordyceps militaris fruit body scraps or waste, dry, crush, and sieve 20-200 mesh; add 0.1%-5% to the culture Base.

[0022] The preparation method of Cordyceps fungus powder B (including bacteria and culture medium): take the pollution-free culture medium after harvesting the fruiting body, which contains a large amount of mycelium, dry it, crush it, and sieve it with 20-200 mesh It can be added to the culture medium by 1% to 20%.

[0023] Under aseptic conditions, get the Cordyceps militaris bacterial classification liquid 5ml, add the substratum (embodiment 1, embodiment 2, embodiment 3, embodiment 4) and common culture medium (comparative example 1) prepared by the present invention that have sterilized ) each in parallel with 5 groups, with more than 20 bottles in each group. Cultivate under the same temperature, humidity, and light conditions, harvest on the 50th day from the date ...

Embodiment 1

[0027] 1. Take the formula: 30g of wheat, 1g of Cordyceps powder B (bacteria powder made from culture medium after picking), 50ml of nutrient solution, bottle, seal, and sterilize by high-pressure steam at 121°C for 30 minutes. The culture medium for obtaining the fruiting body of Cordyceps militaris; the preparation method of the nutrient solution: 3 g of peptone, 2 g of potassium dihydrogen phosphate, 2 g of magnesium sulfate, 10 mg of vitamin B1, and 1000 g of drinking water.

[0028] 2. Under sterile conditions, inoculate the culture medium of Cordyceps militaris, seal the inoculated Cordyceps militaris cultivation bottle, and move it into a sterilized cultivation room, keep the temperature at 18-20°C, and shade with a cloth.

[0029] 3. After the hyphae cover the surface of the culture medium, puncture 1 to 4 small holes in the film of the culture bottle to increase the oxygen supply and start to see the light to stimulate the formation of the primordium of the fruit body ...

Embodiment 2

[0033]Take the formula: 35g of wheat, 2g of Cordyceps fungus powder B (bacteria powder made from the culture medium after picking), 50ml of nutrient solution, bottle, seal, and sterilize under high pressure at 121°C for 30 minutes to obtain the fruiting bodies of Cordyceps militaris Base, nutrient solution preparation method: peptone 3g, potassium dihydrogen phosphate 2g, magnesium sulfate 2g, vitamin B1 10mg, drinking water 1000g. Cultivate Cordyceps militaris according to the method of Example 1. After the fruiting body is harvested, the product output, cordycepin content and adenosine content are all greatly improved, the yield has increased by nearly 25%, and the cordycepin content has increased by nearly 27%. , the adenosine content increased by nearly 15%. See Table 1 for specific data.

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Abstract

The invention relates to a cordyceps militaris fruit body culture medium, mainly comprising a starch substrate and inorganic salt containing phosphorus, potassium and magnesium ions. The cordyceps militaris fruit body culture medium is characterized by further comprising cordyceps fungi powder which is powder containing cordyceps militaris or powder containing cordyceps militaris mycelium. The invention also provides a preparation method of the cordyceps militaris fruit body culture medium. The raw material of zhao cordyceps fungi powder as the important components of the culture medium is cultured waste material, namely culture medium after the cordycepin is cultured or waste material of the fruit body, and therefore, the production cost is reduced. The cordyceps militaris fruit body cultured by using the culture medium, the grown fruit body has excellent quality, and the content of adenosine and cordycepin as effective components is obviously improved compared with the common culturemedium.

Description

technical field [0001] The invention relates to a culture medium for cultivating biological bacteria, in particular to a culture medium for fruiting bodies of Cordyceps militaris and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] Cordyceps militaris, whose scientific name is Cordyceps militaris, also known as Cordyceps militaris, Cordyceps militaris, etc., is a model species of Ascomycota subphylum, Ergotaceae, Ergotaceae, and Cordyceps genus. Cordyceps militaris is a high-quality large fungus that can be used both as medicine and food in my country. The fruiting body contains active ingredients such as cordycepin, cordyceps polysaccharide, SOD enzyme, etc. It has various physiological functions such as enhancing immunity, anti-tumor, and anti-fatigue. Due to the limitation of natural growth conditions, the number of natural products is very limited, and artificial cultivation must be carried out in order to meet people's...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
Inventor 朱旭伟虞利东
Owner 浙江天使生物工程有限公司
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