Method for culturing mycelia and sporocarps of aweto

A technology of Cordyceps sinensis and its cultivation method, which is applied in the fields of botanical equipment and methods, horticulture, application, etc., and can solve the problems that artificial Cordyceps sinensis cannot be cultivated on a large scale.

Active Publication Date: 2010-09-29
杨毅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem that the existing artificial Cordyceps sinensis cannot be cultivated on a large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: the cultivation method of Cordyceps sinensis mycelium and fruiting body, the steps are as follows, and the ratio of each composition is calculated by specific gravity:

[0032] 1. The selection of strains, the test tube species of the following two asexual generations

[0033] Cephalosporium sinensis chen sp nov.

[0034] Isaria cretacea.【Isaria cretacea.】

[0035] 2. The strains need to be experienced and rejuvenated. The obtained strains should be transferred to the PDA test tube culture medium, cultivated at an appropriate temperature (17°C±1°C) for 7 days, then placed in a high temperature of 36°C, a low temperature of -20°C, Smoke fumigation, ultraviolet irradiation, and experience in the environment of mixed bacteria infection can strengthen the training of the bacteria, and the bacteria that die during the rejuvenation process are regarded as degenerated species and discarded; the vigorous growth of the non-degraded strain can be used. Place in ea...

Embodiment 2

[0052] The formula of the solid medium is as follows: 30 parts of corn dregs, 8 parts of soybean flour, 20 parts of wheat grains, 7 parts of sorghum grains, 3 parts of silkworm chrysalis powder, 8 parts of whole eggs, 24 parts of black soybean meal with skin, 5 parts of defatted flax seed dregs Part, all the other steps are with embodiment 1.

[0053] Product quality: the total efficiency of fruiting body and mycelium obtained is 40%, dry product 3.2g, (fruiting body 2g, mycelium 1.2g), after testing, fruiting body contains adenosine=0.021%, contains cordycepin=0.15 %, mycelium contains adenosine = 0.062%, the fingerprint test results show that its gene similarity is 97.5%, the acute toxicity test and long-term toxicity test show that it is non-toxic, and it can be a substitute for natural Cordyceps.

Embodiment 3

[0055] The formula of the solid medium is as follows: 35 parts of corn dregs, 2 parts of soybean flour, 18 parts of wheat grains, 10 parts of sorghum grains, 2.5 parts of silkworm chrysalis powder, 10 parts of whole eggs, 30 parts of black soybean meal with skin, 4 parts of defatted flaxseed oil Part, all the other steps are with embodiment 1.

[0056] Product quality: the total efficiency of the fruiting body and mycelium obtained is 40%, dry product 3.1g, (2.1g of fruiting body, 1g of mycelium), the fruiting body and mycelium of gained are tested, and fruiting body contains adenosine=0.022 %, containing cordycepin = 0.12%, mycelia containing adenosine = 0.056%, the fingerprint test results show that its gene similarity is 97.3%, the acute toxicity test and long-term toxicity test show that it is non-toxic, and can be a substitute for natural Cordyceps Taste.

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Abstract

The invention belongs to the technical field of fungus culture, particularly to a method for culturing mycelia and sporocarps of aweto, which solves the problem that artificial aweto in the prior art can not be cultured in a large scale. The method comprises the following steps of: selecting cephalosporium acremonium and Isaria cicadae; preparing a cephalosporium acremonium liquid strain and an Isaria cicadae liquid strain; preparing a solid culture medium from corn grit, soybean flour, wheat grains, sorghum grains, silkworm chrysalis meal, whole chicken eggs, black bean coarse powder containing hulls, degreased linseed oil and a nutrient solution, wherein the nutrient solution comprises chicken blood, pine needles, carrots, black beans and cattle bone; evenly mixing, and inoculating; and culturing to obtain the sporocarps and the mycelia. The detection indicates that the sporocarps contain at least 0.02% of adenosine and at least 0.1% of cordycepin, and the mycelia contain at least 0.055% of adenosine; the finger-print test result indicates that the degree of similarity of the sporophores and the mycelia is greater than 97%; and acute toxicity experiments and long-term toxicity experiments indicate that the sporophores and the mycelia have no toxicity and can be used as substitutes of natural aweto.

Description

technical field [0001] The invention belongs to the technical field of fungal cultivation, and in particular relates to a method for cultivating Cordyceps sinensis mycelium and fruiting bodies. Background technique [0002] Since the 1980s, fungal workers have isolated multiple species of Cordyceps fungi (such as cephalosporins, bunches, mori, cephalospora, etc.) from natural Cordyceps, and connected the strains to bat moth larvae or artificially cultivated them. Basically, Cordyceps can grow on the larvae and on the medium, and the active ingredients of its hyphae and sporosomes are comparable to those of natural Cordyceps, and some ingredients are higher than natural ones, which proves that artificial Cordyceps can replace Natural Cordyceps, however, after more than 20 years, the artificial compound Cordyceps still stays in the laboratory. Some enterprises have developed the production of Cordyceps sinensis to a large scale by means of large-tank fermentation, but the inv...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 周文贵周翔胡素华
Owner 杨毅
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