The invention discloses a domestic fungus medium, a preparation method thereof and a domestic fungus culture method. The domestic fungus medium comprises, in parts by weight, 75-86 parts of wood chips, 12-20 parts of wheat bran, 1-5 parts of a yeast fermentation product and 0.5-2 parts of gypsum, wherein in the yeast fermentation product, the weight content of organic matter is 50% or more, the weight content of nitrogen phosphorus potassium nutrients is 10% or more, and the weight content of amino acid is 6% or more. By adding the yeast fermentation product into the medium for replacing sugar and part of wheat bran in a conventional domestic fungus medium, the production cost of domestic fungus is substantially reduced when high-quality domestic fungus is guaranteed to be cultured. The domestic fungus medium is low in polluting rate, the yeast fermentation product is high in contents of organic matter, amino acid and nitrogen phosphorus potassium nutrients, beneficial microbes and granular organic fertilizer in assistant materials can satisfy strain growth of domestic fungus, bacteria fermentation speed is fast, bacteria stick quality is good, and mycelia grow densely, are strong in growing power, high in received luminosity and good in disease prevention performance.