434 results about "Fungus culture" patented technology

The invention provides an artificial inoculation production method, which relates to an artificial inoculation method of Jinhua fungus of tea and a processing method of camellia nitidissima. The invention is characterized in that the artificial inoculation production method comprises the following steps of preparation of Jinhua fungus culture solution, preparation of inculating tea, artificial inoculation of Jinhua fungus, culture of Jinhua fungus, drying of products and the like. The invention has high flowering rate of the tea, uniform flowering, coarse grain of eurotium cristatum and golden color, can bring the flowering stage forward by about 3 days, can obviously improve the quality and production efficiency of the camellia nitidissima, is applicable to flowering of all teas (compressed tea and loose tea) and provides reliable guarantee for improving flowering quality of black tea.

The invention discloses a pasania fungus culture substrate consisting of solid materials and water. The solid materials comprise the following components by weight percentage: 35 to 40 percent of summer cut mulberry twig chips, 35 to 40 percent of cotton straw chips, 18 to 20 percent of bran coat, 2.5 to 3 percent of corn flour, 1 to 1.2 percent of sugar, and 1.5 to 1.8 percent of gesso. The dosage ratio of the solid materials to the water is 1: (1.2-1.3). The substrate is packed by bags with the bag diameter of between 18 and 22 centimeters. The substrate is used for culturing pasania fungus, and can make full use of local discarded crop resources in accordance with local conditions without consuming wood. The substrate can reduce the cost and effectively improve the quality and yield of the pasania fungus. The economical benefit is improved by 15 to 20 percent than that of the prior pasania fungus culture substrate; therefore, the substrate has important popularization value.

Invention relates to biotechnology, food industry and concerns to microbial consortia and microbial and yeast strains, as well as to methods for producing by integrated technological cycle with the use of consortia and microbial and yeast strains from the fermented base, which is a semi-product of bread kvass, fermented kvass, nonalcoholic kvass, as well as to methods for producing tea fungus culture fluid, tea-fungus concentrates, kombucha beverages, and vegetable extracts in a single technology process.

The invention relates to Trichoderma harzianum Rifai WRF-2 and use thereof. The bacterium strain was preserved in China General Microbiological Culture Collection Center on January 21st, 2009, with a preservation number of CGMCC No.2870. The bacterium belongs to deuteromycetes and is a lower fungus that is short in culture period and quick in growing and propagating speed and produces a large amount of uniform culture liquid for lignin degrading enzyme-generating bacteria in a short time compared with a major part of basidiomycetes to which whiterot fungi belong. The invention also provides a method for culturing and producing lignin peroxydase (Lip) and manganese peroxidase (MnP) by the fermentation of the bacterial strain in a liquid enzyme-generating culture medium, which can produce high-acitivity LiP and MnP in a short time, and is simple in fermentation process, stable, low in cost, and high in yield.

The invention discloses paecilomyces lilacinus and a cultivating method thereof and applications thereof. The method comprises steps of: A. activation of paecilomyces lilacinus PL461, which comprises steps of: placing paecilomyces lilacinus PL461 bacterial strains on a PDA medium board, cultivating the paecilomyces lilacinus PL461 bacterial strains, and activating the paecilomyces lilacinus PL461 bacterial strains; B. liquid fermentation cultivation of the paecilomyces lilacinus PL461, which comprises steps of: putting the activated paecilomyces lilacinus PL461 bacterial strains into an Erlenmeyer flask containing a liquid medium of PDB and Czapek's, cultivating the above bacterial strains with shaking, and taking the above cultivated bacterial strains as standby; C. solid fermentation cultivation of the paecilomyces lilacinus PL461, which comprises steps of: mixing a solid fermentation cultivation medium uniformly according to a volume ratio, wherein the medium comprises dry compost rice bran, corn flour and water, putting the mixed medium into a domestic fungus culture bag, inoculating the paecilomyces lilacinus PL461 fermented bacteria solution into the solid fermentation cultivation medium employing culture dish fermentation after disinfection and cooling, and putting the above inoculated solid fermentation cultivation medium into a culture dish after stirring uniformly again to cultivate and take as standby; and D. preparation of a paecilomyces lilacinus bacteria agent from the solid phase culture according to a formula and the method. The paecilomyces lilacinus has an obvious lethal effect on nematode eggs, equipment is simple, production cost is reduced greatly, and the cultivating method is suitable for mass production of paecilomyces lilacinus.

The invention relates to the technical field of edible and medicinal fungus culture, in particular to a wild imitating culture collection method for lucid ganoderma and lucid ganoderma conidial powder. The method comprises eight work procedure steps including (1) strain selection; (2) culture environment preparation; (3) culture material preparation; (4) bag sheathing and sterilization; (5) inoculation and fungus growing; (6) land preparation and soil covering; (7) lucid ganoderma growth and culture; and (8) powder collection through sleeve barrels. The wild imitating culture collection method for lucid ganoderma and lucid ganoderma conidial powder provided by the invention has the advantages that through the full improvement on the work procedure steps, the completely imitated wild lucid ganoderma survival environment and internal hereditary basis as well as external substance basis are provided for culturing and collecting high-yield and high-quality lucid ganoderma conidial powder, the cultured and collected lucid ganoderma conidial powder has high yield and high quality, the grains are full, and the purity is as high as more than 99.9 percent.

The invention relates to a manufacture method of carbonized particle nutrition soil for culturing arethusa plants. According to the method, clay powder and organic powder of crop straws, fungus culture waste materials, sawdust or grass scraps or sugarcane slag and the like are mixed into mixed materials; clean water and nutrition elements required by arethusa plants are added and are uniformly stirred; the materials are pressed into solid particles in the size required by the growth of the arethusa plants by a granulating machine; and the solid particles are subjected to dry distillation and carbonization in a sealed container to obtain carbonized particle nutrition soil; or the mixed materials are pressed into the particles without adding the nutrition elements, and the particles are subjected to dry distillation and carbonization and are then soaked by nutrition element solution to be made into the carbonized particle nutrition soil. The carbonized particle nutrition soil prepared by the method provided by the invention has the advantages that the culture substrate and the nutrition ingredients required by the arethusa plants are integrated, the special requirement of the arethusa plants on the culture substrates is met, the nutrition substances can be continuously provided for the arethusa plants, the selection and the ingredient matching of plant materials in the arethusa plant culture are simplified, and the culture method of the arethusa plants is standardized.

The invention belongs to the technical field of fungus culture, particularly to a method for culturing mycelia and sporocarps of aweto, which solves the problem that artificial aweto in the prior art can not be cultured in a large scale. The method comprises the following steps of: selecting cephalosporium acremonium and Isaria cicadae; preparing a cephalosporium acremonium liquid strain and an Isaria cicadae liquid strain; preparing a solid culture medium from corn grit, soybean flour, wheat grains, sorghum grains, silkworm chrysalis meal, whole chicken eggs, black bean coarse powder containing hulls, degreased linseed oil and a nutrient solution, wherein the nutrient solution comprises chicken blood, pine needles, carrots, black beans and cattle bone; evenly mixing, and inoculating; and culturing to obtain the sporocarps and the mycelia. The detection indicates that the sporocarps contain at least 0.02% of adenosine and at least 0.1% of cordycepin, and the mycelia contain at least 0.055% of adenosine; the finger-print test result indicates that the degree of similarity of the sporophores and the mycelia is greater than 97%; and acute toxicity experiments and long-term toxicity experiments indicate that the sporophores and the mycelia have no toxicity and can be used as substitutes of natural aweto.

The invention relates to a high efficiency environmental protection method for culturing tuckahoe, which belongs to the technical field of medicinal (edible) fungus culture. The method comprises the following steps: a, selecting a pine lateral branch with the diameter of between 3 and 10cm to prepare circular or polygonal slices; immersing the slices in water for 12 hours, taking out the slices and draining off the water, and controlling the water content to 70 percent; then packaging the slices into an edible fungus culture bag; carrying out the sterilization for 12 hours at the room temperature or for 2 hours at 120 DEG C, and carrying out the inoculation when the temperature inside the bag is reduced to 25 DEG C; carrying out the culture at 25 DEG C for 30 days for standby; b, selecting a culture wood billet, digging a pit, and carrying out the sterilization; c, placing the culture wood billet into the sterilized pit, taking out the tuckahoe culture seed prepared in the step a, and nailing the culture seed slices onto the sides and cross sections of the culture wood billet through stainless steel nails; d, closely covering the inoculated culture wood billet with a degradable plastic thin film, and covering 20cm soil; and e, carrying out the culture management till harvest according to the conventional tuckahoe. The method has the advantages of simple operation, quick fungus emergence, high inoculation survival rate, and reduced soil environmental pollution. The method can be widely applied to the large-scale tuckahoe production and culture.

The invention belongs to the field of utilization of organic matters, and in particular relates to an edible straw rotting fungus culture medium and a preparation method thereof. The edible straw rotting fungus culture medium comprises the following components in percentage by weight: 40 to 50 percent of primary fungus chaff, 30 to 50 percent of xylitol residue, 5 to 20 percent of bran, 0.2 to 2 percent of calcium superphosphate, 0.2 to 1 percent of corn flour and 0.5 to 1 percent of gypsum. Lime milk and water are added into the medium, the pH is adjusted to be 8 to 10, and the water content is controlled to be 55 to 70 percent. The edible fungus culture medium is high in hypha running speed, hypha exuberantly grows, and the sundry fungus contamination rate can be greatly lowered; produced edible fungi have various nutritional characteristics of edible fungi produced by an ordinary culture material, and have a high edibleness and medicinal value.

The invention discloses a universal edible fungus liquid fungus culture medium.Its weight ratio of the formula is: malt dextrin 45-55 portions, dextrose 10-15 portions, cane sugar 10-15 portions, yeast extract paste 1-1.5 portions, peptone 1-1.5 portions, potassium di-hydrogen phosphate 2-3 portions, bitter salt 1-1.5 portions, and water 1000 portions. The invention also discloses the technology that includes following steps: heating water to 90-95 degree centigrade, adding malt dextrin and whisking until fully solving, adding dextrose, cane sugar, yeast extract paste, and peptone and whisking until fully solving, adding potassium di-hydrogen phosphate and bitter salt and fully mixing to make mixture liquid, which would take high temperature and high pressure sterilization. The invention has strong versatility and short fermentation period.

The invention relates to an edible fungus automatic production line and belongs to the technical field of edible mushroom production. The edible fungus automatic production line comprises an electric automatic blending and weighing system, a sterilization pot, an edible fungus automatic inoculation machine, a spiral automatic bagging machine and a bagging conveying mechanism. A discharge port of the electric automatic blending and weighing system is placed above a sterilization pot feed port assembly, the sterilization pot feed port assembly is connected with a culture medium feed port of the spiral automatic bagging machine, the edible fungus automatic inoculation machine is connected with a culture feed port of the spiral automatic bagging machine, and a mixed discharge port of the spiral automatic bagging machine is connected with the bagging conveying mechanism. The edible fungus automatic production line can achieve automatic blending, automatic inoculation, automatic bagging and automatic packaging, saves manual work, improves fungus culture speed and yield, reduces the building area of an edible fungus plant, and improves product quality, and edible fungus growth speed and size are uniform in subsequent handling, and management is benefited.

The invention discloses a method for culturing Cordyceps militaris by using highland barley as a main raw material, belonging to a fungus culture method in the biotechnology, in particular relates to a method for artificially culturing the fungus, and particularly relates to a method for artificially culturing Cordyceps militaris. The method comprises the following steps of preparing nutrient solution, preparing highland barley growing culture medium, preparing liquid strain, inoculating, culturing, harvesting and the like. The method has the beneficial effects that the highland barley is used as a medium raw material to culture Cordyceps militaris, and the nutrients of the highland barley are absorbed by Cordyceps militaris in the growth phase, so that the content of the effective ingredient cordycepin in the Cordyceps militaris is improved to 1% in the method from the original 0.5%, the content of effective ingredient cordycepic acid is improved to 9% of the product from the existing 6.1%, the content of effective ingredient cordyceps polysaccharide is improved to 10.5% of the product from the existing 7.4%, and the content of the effective ingredients of the Cordyceps militaris is totally improved by 200-250%; and the highland barley is used as the medium raw material to culture the Cordyceps militaris, and thus the obtained Cordyceps militaris has dual effects of the highland barley and the Cordyceps militaris.

The invention relates to a culturing method for edible mushrooms by taking honeysuckle as culture medium. The culture medium of the edible mushrooms is crushed rootstock and flower disc of honeysuckle added with wheat bran, wherein ratio between the honeysuckle components and the wheat bran in terms of weight percentage is 75-85%: 15-25%. The edible mushroom is black fungus, or dried mushroom, or plelmtus eqngiu, or Coprinus comatus, or siberian elm yellow (Jade Emperor) mushroom. The edible mushrooms cultured by honeysuckle as culture medium contain healthy component, chlorogenic acid compound which has functions of bacteriostasis, resisting viral, allaying a fever, resisting nflammatory, protecting liver, stopping bleeding, anti-oxidation and adjusting immune. But other edible mushrooms have no the functions. The mushroom such as black fungus cultured by honeysuckle as culture medium can be mixed in hot water. The mixed water can diminish inflammation, reduce phlegm and have a certain effect of treating Otorhinolaryngeal Diseases.

The invention belongs to the technical field of domestic fungus production, relates to a domestic fungus culture material and especially relates to an edible fungus culture medium and a preparation method thereof. The edible fungus culture medium is prepared from tung tree sawdust, tung tree seed hull, bagasse, lime, gypsum, sugar cane bagasse and a nutrition additive. Each 1000g of the nutrition additive is prepared from 0.2-0.5 parts by weight of manganese sulfate, 0.2-0.5 parts by weight of ammonium molybdate, 0.5-1.5 parts by weight of calcium superphosphate, 0.3-0.8 parts by weight of copper sulphate, 0.2-0.6 parts by weight of zinc chloride, 0.1-0.5 parts by weight of selenium potassium phosphate, 4-10 parts by weight of diatomite, 1-3 parts by weight of honey and 2-5 parts by weight of cane sugar juice. The preparation method of the edible fungus culture medium has simple processes. The product of tung tree is used for preparation of the edible fungus culture medium, can be fermented fast, contains lasting-action nutrients, does not pollute the environment, has no pesticide residue and no heavy metal residue and is conducive to domestic fungus production.

The invention discloses a domestic fungus medium, a preparation method thereof and a domestic fungus culture method. The domestic fungus medium comprises, in parts by weight, 75-86 parts of wood chips, 12-20 parts of wheat bran, 1-5 parts of a yeast fermentation product and 0.5-2 parts of gypsum, wherein in the yeast fermentation product, the weight content of organic matter is 50% or more, the weight content of nitrogen phosphorus potassium nutrients is 10% or more, and the weight content of amino acid is 6% or more. By adding the yeast fermentation product into the medium for replacing sugar and part of wheat bran in a conventional domestic fungus medium, the production cost of domestic fungus is substantially reduced when high-quality domestic fungus is guaranteed to be cultured. The domestic fungus medium is low in polluting rate, the yeast fermentation product is high in contents of organic matter, amino acid and nitrogen phosphorus potassium nutrients, beneficial microbes and granular organic fertilizer in assistant materials can satisfy strain growth of domestic fungus, bacteria fermentation speed is fast, bacteria stick quality is good, and mycelia grow densely, are strong in growing power, high in received luminosity and good in disease prevention performance.

The invention discloses a ganoderma lucidum culture medium, a method for cultivating ganoderma lucidum, ganoderma lucidum tea and a preparation method of the ganoderma lucidum tea, and belongs to edible or medicinal fungus culture media. The culture medium is formed by mixing hawthorns, wheat germs, salviae miltiorrhizae, astragalus membranaceus, licorice roots, sugar and bean pulp. The raw materials of the culture medium are free of pollution and cannot generate the toxic action on the human body, and the polysaccharide content, the triterpene content and the content of flavonoid materials of the ganoderma lucidum cultivated through the culture medium are more than those of ganoderma lucidum cultivated through an existing culture medium.

The present invention presents an alternative to the direct implementation of an industrial scale second generation bioethanol process with the integration of the second generation into the existing first generation bioethanol processes, which aims to reduce the current barriers to process change / investments. In particular, the present invention relates to an integrated second generation process for producing bioethanol comprising at least one fungal cultivation stage for producing ethanol and fungal biomass. The present invention also relates to a novel fungal biomass, rich in protein and essential amino acids, which is produced with said integrated second generation process for producing bioethanol for use as a nutritious substitute for human and domestic animal use.

The invention relates to an establishing method of symbiont of cyclobalanopsis glaucoides and bolete, belonging to the field of biotechnology (plant tissues and fungal culture). In the research of mycorrhizal fungi, a stable pure symbiont of plants and fungus is difficult to establish. At present, bolete can not be cultivated artificially easily, belongs to mycorrhizal fungi capable of realizing mutualistic symbiosis with the plant, and has symbiotic relationship with pinaceae plants, fagaceae plants and the like under natural condition. According to the establishing method, cyclobalanopsis glaucoides seed and generalized delicious beef liver mushroom entity collected in the Wuding lion rock are used as materials, so that callus and mycelium are respectively induced successfully and are cultured together in an innovation manner, and a simple and stable pure-symbiont system is established under the sterile state. Important basis is provided for the symbiotic relationship and the symbiotic mechanism of bolete, cyclobalanopsis glaucoides and the other plants and the molecular mechanism of the beef liver mushroom entity and the like, and the method use for reference is provided for research on other macro fungi and symbiotic plants.

The invention relates to a high-yield and fast cultivation technology of pleurotus edible fungus. The technique thereof comprises the steps of molding, treatment of culture materials, preparation of a fungus pier, fungus culture and picking. The prepared fungus pier reasonably utilizes the space, adopts three-dimensional cultivation, and has small occupied area. In the process of preparing the fungus pier, a round hole is reserved at the middle for filling soil and injecting water; the prepared fungus pier is covered by a plastic film with larger diameter than that thereof; when in early-period fungus culture, hypha germinates rapidly and absorbs the material fast since oxygen penetrates through the middle and the periphery of the whole culture fungus pier and can block the material surface in short time so as to effectively prevent competitors from invading; and when in final-period management, muddy soil and clean water are filled in the round hole at the middle of the fungus pier, and enough and balanced water guarantees the yield. In the high-yield and fast cultivation technology, a mold device has simple manufacture, low cost and convenient operation and use; the fungus pier has novel, reasonable and unique structure, therefore, the high-yield and fast cultivation technology greatly simplifies the existing production technique, improves production efficiency, and has the advantages of low pollution rate, fast fungus running speed and the like.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

The invention discloses a method to screen selecting fungi Trichoriella ornithopoda Oorschot et de Hoog culture water and calcia chloridum mixed freezing natural rubber latex from waste disposal basin of producing natural rubber; which is characterized by the following parts: shortening time of biological freezing; the time of biological freezing shorten from 6 hours to 3min- 2h and rationalize property of product is better than acid freezing. The invention is fit for rubber-clear freezing and freezing of low ammonia latex with ammonia quantity less than 0.04%.

The invention discloses a biologically fermented fungus bran protein feed additive. The invention adopts the technical scheme that the biologically fermented fungus bran protein feed additive is prepared by the following steps: drying and crushing edible and medicinal fungus bran, heating, carrying out reflux extraction, concentrating to prepare a concentrated solution, and then utilizing 88-92% of the fungus bran extraction concentrated solution and 8-12% of a compound bacteria leavening agent, carrying out culture and fermentation for 48-100 hours according to the conditions that the temperature is 25-35 DEG C, the stirring rate is 45-120 r / min, and the ventilator capacity is 1: (0.5-1.5) v / v per minute, and at last carrying out spray drying to obtain the product. The biologically fermented fungus bran protein feed additive obtained by the method disclosed by the invention has the crude protein content of 42-48%, so that the nutrition of the additive can be greatly promoted, the plant protein grain resources can be reduced, the waste fungus bran is turned to be useful, and the economic benefit of the industrial chain from edible and medicinal fungus culture to biologic protein feed processing to livestock healthy breeding can be realized; the method is simple in process, low in production cost, obvious in economic benefit, and good in application prospect.

A nutritive health-care powder for improving immunity and preventing diseases is prepared from the tartarian buckwheat as culture medium and the cordyceps fungus cultured in said culture medium through culturing, fermenting and processing. Its advantages are rich nutrients including rutin, quercetin, 3-deoxynucleotide, Se, Ca, etc and simple preparing process.

The present invention relates to the cultivation method of high temperature pleuotus nebrodensis Quel Tianshan 619 as one new variety of mushroom, and belongs to the field of fungus culture technology. The high temperature pleuotus nebrodensis Quel Tianshan 619 is cultured with wild Tianshan mountain pleuotus nebrodensis Quel and through purification and high temperature domestication. It has large and white sporophore of 6-16 cm diameter and 3-7 cm thickness and colorless smooth spore. The culture process includes compounding culture medium, inoculation and field culture, and has the advantages of high yield, high mushroom quality, low cost and other advantages.

The invention is suitable for the field of biopharmaceutics, and provides a fungus culture extract and a preparation method and application thereof. The extract is a cyclic dipeptide compound which can be separated from a deep sea fungus fermentation culture of which the number is CCTCC M 2015628. The cyclic dipeptide compound has the high activity of inhibiting generation of nitric oxide (NO), has no toxicity on cells and can be applied to preparation of medicine for treating an Alzheimer disease.

The invention relates to a low-carbon and high-yield poria culture method and belongs to the technical field of edible (medicinal) fungus culture. According to the method, poria is cultured naturally by utilizing underwood waste pine root and stem, and a specific poria strain, which is Wolfiporia extents Chuanjie-1-A5 with preservation number of CGMCC No.6660, suitable to be cultured by using pine root and stem is selected and is cultured in combination with a taproot inoculation method. The culture method comprises the following steps of: producing a strain; pre-treating the pine root and stem; inoculating; and conventionally managing and harvesting. According to the method, waste pine root and stem of forestry is effectively utilized to naturally culture poria in a low-carbon manner, 'conflict between fungus and forest' in poria production can be effectively solved, and harm of termite and other insect pests caused by forest waste discharge can be reduced, so that the production cost can be reduced, labor intensity of poria planting can be greatly reduced, time and labor can be saved, and management is facilitated. The culture method is beneficial to sustainable development of the poria industry and has remarkable economical benefit.

The invention relates to the field of edible mushrooms and particularly relates to an artificially-bred bamboo fungus culture medium. The bamboo fungus culture medium comprises the following components in parts by weight: 20-25 parts of bamboo bits, preferably bamboo rhizome bits with the age of over two years, 10-20 parts of brown wood bits, 10-15 parts of bamboo leaves, 10-15 parts of bamboo forest humus soils, 2-5 parts of potatoes, 5-10 parts of bagasse, 10-15 parts of wheat straws, 2-5 parts of soybean meal, 0.3-0.5 part of glucose, 0.5-1 part of calcium superphosphate, 0.5-1 part of magnesium sulfate, 0.5-1 part of ammonium nitrate and 0.5-1 part of hydrated lime, wherein the bamboo leaves and the wheat straws are soaked and bubbled for over 30 days by use of water in a fermenting tank, properly dried in the air after being bubbled until the moisture content is 25%-35%, wherein the integral moisture content of the culture medium is 70%-80%; and the culture medium is pressed by beating and compacted, so that the porosity is smaller than 10%. The culture medium is complete in nutritional ingredient, moderate in acid and alkaline condition, and capable of meeting the growth needs of the bamboo fungus and greatly increasing the yield of the artificially-bred bamboo fungus.

The invention provides a field cultivating black fungus culture medium in Tibet region and a cultivation method for the field cultivating black fungus in Tibet region, and relates to black fungus culture medium and the cultivation method for black fungus. The invention solves the problems that the material for artificially cultivating black fungus in interior areas is not applicable to culturing and producing agaric in Qinghai-Tibet Plateau, and such material is hardily obtained in the Qinghai-Tibet Plateau, and meanwhile, the cultivation technology and experience in the interior areas cannot be carried out in Tibet region properly. According to the cultivation method, the field cultivating black fungus culture medium in Tibet region is prepared by 32% to 38% of solid raw material and 62% to 68% of water in percentage by weight. The cultivation method comprises the steps of: 1, preparing the culture medium; 2, sub-packaging, sterilizing, culturing and storing; 3, manufacturing a bed, and culturing with bacteria trapped; 5, culturing a bacteria bed; and 5, individually managing and culturing. The cultivation method provided by the invention is applied to culturing the black fungus in Tibet region.

An annual cultivation method for coprinus comatus sequentially comprises the following production methods: the technological processes of culture production, fermentation treatment of culture medium, sterilizing inoculation, hairy fungus culture, soil sealing, mushroom production, and the like, are carried out; the culture is produced by a liquid seed production method; reed residues and needle mushroom leftovers are used as culture medium for fermentation treatment; cultural bags adopts open type inoculation, and a two-stage type cultural method is used; the inoculation and the hairy fungus culture are carried out in ordinary steel tube greenhouse; the soil sealing and mushroom production are carried out in the environment of civil air-defense construction. The invention utilizes idle civil air-defense facilities, does not need refrigeration plants without energy consumption and can always keep the environmental temperature of mushroom production at 18-22 DEG C, thereby being capable of not only ensuring coprinus comatus to grow normally but also controlling the generation of important disease palmatum bacteria of coprinus comatus so as to efficiently realize annual production of coprinus comatus; in addition, the mushroom production time can be freely controlled by controlling the soil sealing time so that the biological conversion rate is more than 80% and the yield can be improved more than 25% compared with ordinary cultivation.