46 results about "Isaria cicadae" patented technology

The invention relates to the field of biotechnology and discloses a molecular identification method for Isaria cicadae (CGMCCNO.3453). The method includes the steps of extracting genomic DNA of the strain, performing amplification of the DNA in the transcription spacers of the genome using primers ITS5 and ITS4, and recovering and purifying the obtained DNA fragments; performing bidirectional sequencing of the purified DNA fragments; aligning the obtained DNA sequences with SEQIDNO:3; and identifying the strain as Isaria cicadae (CGMCCNO.3453) if the ITS sequence of the strain has the same content and order of arrangement as SEQIDNO:3. The inventive method can rapidly, reliably and accurately identify Isaria cicadae (CGMCCNO.3453), and the identification method is not influenced by environmental factors.

The invention belongs to the technical field of fungus culture, particularly to a method for culturing mycelia and sporocarps of aweto, which solves the problem that artificial aweto in the prior art can not be cultured in a large scale. The method comprises the following steps of: selecting cephalosporium acremonium and Isaria cicadae; preparing a cephalosporium acremonium liquid strain and an Isaria cicadae liquid strain; preparing a solid culture medium from corn grit, soybean flour, wheat grains, sorghum grains, silkworm chrysalis meal, whole chicken eggs, black bean coarse powder containing hulls, degreased linseed oil and a nutrient solution, wherein the nutrient solution comprises chicken blood, pine needles, carrots, black beans and cattle bone; evenly mixing, and inoculating; and culturing to obtain the sporocarps and the mycelia. The detection indicates that the sporocarps contain at least 0.02% of adenosine and at least 0.1% of cordycepin, and the mycelia contain at least 0.055% of adenosine; the finger-print test result indicates that the degree of similarity of the sporophores and the mycelia is greater than 97%; and acute toxicity experiments and long-term toxicity experiments indicate that the sporophores and the mycelia have no toxicity and can be used as substitutes of natural aweto.

The invention belongs to the technical field of foods and health-care products, particularly relates to an isaria cicadae miquel beverage, and further discloses a preparation method of the isaria cicadae miquel beverage. According to the isaria cicadae miquel beverage disclosed by the invention, isaria cicadae miquel is used as a main raw material, and medicine effects of rose flowers, mulberry leaves, mint, Chinese wolfberry fruits, licorice roots, mulberriees, Chinese jujubes, longans, haws, Chinese wolfberry fruits and/or rhizoma polygonati are used as auxiliary materials, so that the isaria cicadae miquel beverage can effectively enhance the immunity of human bodies, and has the efficacy of improving sleep, resisting weariness and the like.

The invention relates to a preparation method of beautiful Isaria cicadae wine. The method comprises the followings steps: a step 1: bacterial strains of Isaria cicadae are pressed on the surface of a medium with a porous cover plate in a solid culture process; a step 2: when coremium grows from small pores to a suitable height, coremium at the part where the cover plate and the medium are contacted and the medium are separated; a step 3: the cover plate and coremium which grows in the cover plate are placed in a container together; a step 4: wine is placed in the container; and a step 5: the wine is stored. The preparation method guarantees uniform growth vigor of coremium, eliminates suspension phenomenon of wine due to long time immersion of the medium in the wine, so that quality of the Isaria cicadae wine is guaranteed.

The invention provides a compound EM active beneficial bacteria mother liquor bio-organic liquid fertilizer, which comprises main raw materials of seaweed, kelp, Ganoderma applanatum, Coprinus comatus, pollen, shoestring fungus, Gynostemma pentaphylla, spirulina, Hericium erinaceus, cicada fungus, saffron, Isaria cicadae Miquel, Ganoderma lucidum, and seaweed dietary fiber. The present invention also provides a preparation method of the organic liquid fertilizer and application thereof. The liquid fertilizer can effectively improve the crop root growth environment, promote the root growth, facilitate the absorption of mineral nutrition in soil by the roots, prevent aging of crops, improve yield and quality of the crops, and enhance the disease resistance of crops. For the aspect of poultry feed, due to the role of photosynthetic bacteria, the liquid fertilizer can eliminate odor of feces, and purify the sludge, wastewater and waste residues in pigpen, cattle pen and sheep pen, so that the liquid fertilizer produced from farming can reach the use standards, improve the nutritional effect of liquid fertilizer on plant, and prevent influenza in poultry.

The invention provides isaria cicadae miquel yoghourt. The yoghourt is prepared from fresh milk as the raw material, and through fermentation, a yoghourt base material is obtained, wherein the addition amount of the isaria cicadae miquel is 0.1-0.3% of that of the yoghourt base material. The yoghourt is prepared from the isaria cicadae miquel as the raw material, the isaria cicadae miquel does notneed to be extracted, and after being crushed, the isaria cicadae miquel can be directly mixed with the yoghourt base material, so that the isaria cicadae miquel yoghourt is rich in nutrition, thickin mouth feel, fine and smooth, moderate in sour and sweet degrees, and delicious. The isaria cicadae miquel is good in flavor.

The invention discloses a tonifying red bean cake. The tonifying red bean cake is characterized by comprising the following raw materials in part by weight: 20-25 parts of red bean, 3-4 parts of osmanthus, 1-2 parts of medlar, 1-2 parts of codonopsis pilosula, 1-2 parts of liquorice, 3-4 parts of honey, 5-6 parts of papaya juice, 2-3 parts of isaria cicadae miq, 20-35 parts of flour, 20-30 parts of water and 3-6 parts of soybean oil. In the formulation of the tonifying red bean cake, not only the red bean is included, but also the osmanthus, the medlar, the codonopsis pilosula, the liquorice, the honey, the papaya juice and the isaria cicadae miq with certain proportions are included, so that the tonifying red bean cake is good in mouth feel and fragrant in taste and tonifying components are added; the components in the tonifying red bean cak can generate a synergistic efficacy and can achieve an effect of calming the heart and the mind.

The invention relates to a detection method of isaria cicadae miquel. According to the detection method, high performance liquid chromatography is adopted. The detection method comprises following steps: a, determination of chromatographic conditions, wherein an acetonitrile-dihydric phosphate solvent system is taken as a mobile phase, and isocratic elution is adopted; b, a reference solution is prepared, wherein an adenosine reference substance is mixed with a solvent so as to obtain the reference solution; c, a sample solution to be detected is prepared, wherein an isaria cicadae miquel sample to be detected is mixed with a solvent so as to obtain the sample solution to be detected; and d, measuring is carried out, wherein the reference solution and the sample solution are injected into a liquid chromatograph respectively for measuring. The detection method can be used for detecting isaria cicadae miquel sporocarp, spore powder, mycoplasm, or extracts or preparations of isaria cicadae miquel sporocarp, spore powder, and mycoplasm; the detection method is simple; few influences are caused; and stability is high.

The invention discloses edible mushroom health-care wine and a preparation method thereof, which belong to the technical field of brewing of health-care wine. The invention adopts the technical scheme that the edible mushroom health-care wine comprises the following ingredients: sorghum, corn, wheat, rice, sticky rice, edible mushrooms, Isaria cicadae, cordyceps militaris, white chrysanthemums, radix astragali, fructus lycii, angelica sinensis, fructus schizandrae, lotus leaves, mulberry leaves, radix glycyrrhizae and honey. The preparation method comprises the following steps: fermenting the edible mushrooms alone, fermenting edible mushroom fermentation broth and part of Chinese herbal medicines and cereals, and soaking the Isaria cicadae, the white chrysanthemums and crude medicines of the lotus leaves, the mulberry leaves and the like with raw wine by stages subsequently, so as to form the health-care wine. All the crude traditional Chinese medicines are scientific and reasonable in matching and appropriate in dosage, the fermentation process and the soaking time are appropriate, and the effective ingredients of all the traditional Chinese medicines can be released into Baijiu; the health-care wine has the effects of regulating internal organs of a human body, tonifying Qi, dredging collaterals, balancing yin and yang, relaxing the muscles, stimulating the blood circulation and the like, so that the immunity of the human body is enhanced, and the health-care effect is obvious.

The invention discloses a dendrobium officinale compound preparation and a preparation method thereof, and belongs to the field of pharmacy. The dendrobium officinale compound preparation comprises the following raw materials by weight: 10-35g of dendrobium officinale fruit, 5-25g of isaria cicadae miq, 30-50g of dangshen, 22-40g of astragalus and 8-32g royal jelly. The dendrobium officinale compound preparation has the body's immune regulating function, blood pressure lowering function, blood lipid lowering function and anti-aging function.

The invention discloses an optimized wild saria cicadae Miquel strain liquid artificially-culturing method. A liquid culturing medium in an optimized formula is adopted and comprises 20 g of solubility starch, 10 g of milk powder, 0.5 g of monopotassium phosphate, 1 g of magnesium sulfate, 10 pieces of vitamins B12 and 1,000 ml of water, wherein the pH of the liquid culturing medium ranges from 5.0 to 6.0. Wild saria cicadae Miquel strains are artificially cultured; on the basis of a traditional basic culturing medium, a systematic and effective optimized culturing mode for screening optimal carbon sources, optimal nitrogen sources and optimal micronutrients during liquid production of wild saria cicadae Miquel strains is provided, and excellent technical guidance is provided for large-scale cultivation of wild saria cicadae Miquel.

The invention relates to an isaria cicadae miquel spore powder artificial culture method. The isaria cicadae miquel spore powder artificial culture meth is characterized by comprising following steps of step 1, making a moisture-absorption carrier be fully contacted with strain seed liquid obtained by liquid culture; step 2, carrying out dark culture on the moisture-absorption carrier contacted with the seed liquid first, and carrying out light culture on the moisture-absorption carrier after the moisture-absorption carrier is full of mycelium; and step 3, harvesting spore powder. According to the invention, a moisture-absorption material is taken as the carrier to culture spore powder, the process is simple, the period is short, the sporulation quantity is high, the quality of spore powder is high, and compared with existing solid culture, the isaria cicadae miquel spore powder artificial culture method has obvious advantages.

The invention relates to a method for artificially culturing Isaria cicadae Miquel. The method comprises the following steps: 1. carrying out amplification culture on an Isaria cicadae Miquel strain; 2. carrying out solid culture on the strain obtained by amplification culture in the step 1; and 3. harvesting sporophores. The method is characterized in that deuterium-depleted water is used in the processes of the amplification culture in the step 1 and solid culture in the step 2, and the content of deuterium does not exceed 100ppm. Due to the adoption of the deuterium-depleted water, the yield and quality of the sporophores of the Isaria cicadae Miquel can be remarkably improved.

The invention discloses a preparation method of isaria cicadae miq vinegar, the isaria cicadae miq vinegar is prepared from isaria cicadae miq as a raw material by two step fermentation, in the isaria cicadae miq vinegar fermentation process, preliminary extraction and conversion of effective components of the isaria cicadae miq can be realized, in the isaria cicadae miq vinegar fermentation process, effective substances can be further extracted, the finally prepared isaria cicadae miq vinegar eliminates the isaria cicadae miq self meat flavor, has the inflammation diminishing and free radical scavenging effect, and can play the anti allergic and anti ageing role.

The invention provides an efficient cultivation method of isaria cicadae miq, which comprises the following steps of: 1) selecting an olive tree orchard with sandy soil, intercropping gynostemma pentaphyllum under olive trees, soaking the egg branches of the isaria cicadae miq in weak acid electrolyzed water and airing under the sun, then transferring the egg branches of the isaria cicadae miq toa heat preservation room, controlling the humidity of the heat preservation room to be 70-8% and the temperature to be 30-35 DEG C, spraying the egg branches once every day by using balsam pear juiceat 6.00 p. m. until golden cicada is hatched and put into the orchard for cultivation; 2) carrying out weeding and insect prevention and topdressing on the gynostemma pentaphylla during the cultivation period of the golden cicada; 3) putting the golden cicada into a cultivation chamber after killing the golden cicada, controlling the oxygen partial pressure, temperature and humidity in the cultivation chamber, and continuously cultivating to obtain the isaria cicadae miq. The method has the advantages of high hatching rate of the golden cicada eggs, and promotion of the transformation of paecilomyces cicadae from a nutrient stage to a sexual stage and the like.

The invention relates to a method for preparing a food for reducing blood sugar by utilizing isaria cicadae miquel for solid-state transformation of Chinese yam and soybean meal. The method comprisesthe following steps of adopting rice, the soybean meal and the Chinese yam as main raw materials, adopting urea as an auxiliary material, sterilizing, then mixing with the isaria cicadae miquel, spreading on a solid-state fermentation bed to carry out solid-state fermentation, and obtaining a fermented substance, i.e., the food for reducing the blood sugar. The method has the beneficial effects that every gram of food for reducing the blood sugar contains 2-30 mg of polysaccharide and 2-10 mg of ergosterol; the food for reducing the blood sugar can reduce the blood sugar obviously, and is a pure-natural preparation; compared with the existing medicines for reducing the blood, the food for reducing the blood sugar not only is fast and obvious in effect, but also cannot cause hypoglycemia after being taken.

The invention provides an active isaria cicadae miquel substance and an application thereof in prevention, delaying or treatment of cataract, and belongs to the technical field of active substances. A preparation method of the active isaria cicadae miquel substance comprises steps as follows: isaria cicadae miquel strains activated in a PDA (potato dextrose agar) slant medium are inoculated into a seed solution medium for culture, and a seed solution is obtained; the seed solution is inoculated into a PDA liquid medium containing traditional Chinese medicine extract and Cajanonic acid A for culture, then mycelia and fermentation liquor are dried and smashed, and a fermented product containing the active substance is obtained; the fermented product is extracted in distilled water, an extracting solution is obtained and concentrated into extractum, the extractum is dried, and the active isaria cicadae miquel substance is obtained. Active ingredients in the active isaria cicadae miquel substance obtained with the preparation method can play the gain role, and the active isaria cicadae miquel substance has a protecting effect, delays the pathological process of posterior capsule opacification, delays development of cataract and is expected to be applied to prevention and treatment of age-related cataract and after cataract.

The invention relates to the application of Isaria cicadae Miquel to preparation of drugs or health products with hpyerglycemic effect. Usage of Isaria cicadae Miquel alone or together with both edible and medicinal traditional Chinese medicines can reduce the contents of total cholesterols, triglycerides and low-density lipoprotein in blood obviously and produce obvious hpyerglycemic effect.

The invention discloses isaria cicadae Miq extractives and application thereof for preparing an antioxidant drug, and belongs to the technical field of drugs. By means of the characteristic that different polysaccharide ingredients of Isaria cicadae Miq have different ethyl alcohol precipitation concentrations, polysaccharide grading separation purification is performed, the method for effectivelyextracting Isaria cicadae Miq polysaccharide ingredients is put forward, and a source is supplied for searching the Isaria cicadae Miq active ingredients with accurate antioxidant activity. Micromolecule polysaccharide with the efficient antioxidant activity is achieved through separation from Isaria cicadae Miq extractives, some protein and other impurities are removed through repeated alcohol precipitation, Isaria cicadae Miq polysaccharide of different molecule structures is effectively separated, the target polysaccharide purity is relatively high, Isaria cicadae Miq polysaccharide with the antioxidant activity is researched, and the polysaccharide utilization rate is increased.

The invention provides a method for drying Isaria cicadae Miquel fruiting body, wherein a belt type drying method is used, the drying temperature is below 70 DEG C, and the drying is performed until the water content in the fruiting body is less than 5%. According to the present invention, with the drying method, the drying cycle can be shortened, the fungus carrying amount of the non-target fungus can be reduced, the energy consumption can be reduced, and the appearance and the intrinsic quality of Isaria cicadae Miquel fruiting body can be improved.

The invention discloses a traditional Chinese medicine composition with the function of reducing blood fat. The traditional Chinese medicine composition is prepared from, by weight, 30-34 parts of rhizoma alismatis, 24-28 parts of isaria cicadae miq, 16-20 parts of lotus leaves, 10-14 parts of hippophae rhamnosides and 10-14 parts of malt. The invention further discloses a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition sufficiently adopts traditional Chinese medicines with the slow medicine property and quite high safety for reasonable combination, the traditional Chinese medicine composition is scientific in compatibility, synergistic interaction is generated, the blood fat is reduced, the better curative effect is achievedcompared with traditional preparations, the quality is more controllable, and the traditional Chinese medicine composition is suitable for industrial production.

The invention provides health care soup and a making method thereof. The health care soup is prepared from the following raw materials and auxiliary materials in parts by weight, wherein the raw materials comprise 400-600 parts of loaches, 8-15 parts of an isaria cicadae miq extract, 4-8 parts of a haw extract, 8-12 parts of a glossy ganoderma extract, 7-13 parts of a radix codonopsis extract, 4-10 parts of an astragalus membranaceus extract, 6-15 parts of an eucommia ulmoides extract and 2-7 parts of a corn stigma extract; the auxiliary materials comprise 5-15 parts of barbary wolfberry fruits, 8-15 parts of red jujubes, 2-5 parts of ginger, an appropriate amount of oil, an appropriate amount of salt and an appropriate amount of cooking wine; the health care soup is prepared through the following steps: mixing the raw materials and the auxiliary materials so as to obtain a mixture, adding water, and decocting the mixture in the water so as to obtain the health care soup. The invention further provides a making method of the health care soup. The health care soup is made through the steps: using the loaches as main food materials, using substances which not only are foods but also traditional Chinese medicine materials as raw materials, and decocting the main food materials and the raw materials. The health care soup disclosed by the invention adopts a traditional Chinese medicine theory, is mutually beneficial in yin and yang, relieves both primary and secondary symptoms, is scientific in concerted application, is reasonable in dosage, has the effects of nourishing yin, promoting salivation, nourishing kidneys, strengthening waists, strengthening tendons and bones, strengthening spleens, replenishing qi, dispelling wind, eliminating dampness and the like, and has the characteristics of reducing body weight and prolonging life after being eaten for a long term.

The invention provides a method for artificial cultivation of Isaria cicadae and a cordyceps cicadae traditional Chinese medicinal material. The Isaria cicadae has a preservation number of CGMCC No.13684, strains of Isaria cicadae are subjected to steps of slant activation culture, seed culture and solid medium cultivation, and a large number of coremia of the cordyceps cicadae traditional Chinesemedicinal material are obtained. The sporulation ability of the Isaria cicadae is extremely strong, the artificial cultivation method is simple and easy, and the coremia as the traditional Chinese medicinal material cordyceps cicadae which is collected in natural forests can be obtained, so that the problem of shortage of a wild cordyceps cicadae medicinal material is solved.

The present invention provides a drying method of isaria cicadae sporocarps. The method is as follows: before drying, firstly the ambient humidity is reduced to 60% or less. The ambient humidity is reduced to 60% or less and then the drying is conducted. The technology can significantly improve the appearance, and color and luster of the products, the various active ingredients are easier to preserve, and the drying method prevents the conversion and loss of the ingredients and enhances the market competitiveness of the products.

The invention relates to the technical field of health-care drinking tea, in particular to an isaria cicadae health-care tea and a preparation method thereof. The tea is prepared from the following raw materials in parts by weight: 100-200 parts of fresh tea leaves, 40-60 parts of isaria cicadae, 10-14 parts of Chinese angelica, 5-9 parts of ginseng, 1-3 parts of dried orange peel, 2-4 parts of magnolia flowers and 4-6 parts of roses. The isaria cicadae health-care tea is simple in preparation method and simple in preparation process, and the functions of the isaria cicadae such as improving immunity, improving kidney function, resisting fatigue, reducing blood fat, reducing blood sugar, lowering blood pressure, resisting tumors, improving eyesight, improving sleep, resisting radiation, relieving pain and tranquilizing mind are melted into the tea, so that the tea has good health-preserving effect and good health-care effect.

The objective of the invention is to provide a method for preparing beer from a residual solid medium of Isaria cicadae Miquel. The method comprises the following steps: with the residual solid mediumof Isaria cicadae Miquel as a raw material, separately extracting alcohol-soluble and water-soluble functional factors; then mixing the functional factors with malt; and carrying out saccharification, cooking of lupulus, fermentation and the like so as to prepare the functional beer with the functional factors of Isaria cicadae Miquel.