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Preparation method and application of isaria cicadae extracting solution

A technology of Cordyceps cicadae and extract solution, which is applied in the field of preparation of Cordyceps cicadae extract, can solve the rare problems of scavenging free radicals by Cordyceps cicadae, improve absorption utilization rate, increase fermentation efficiency, and optimize fermentation conditions Effect

Active Publication Date: 2019-10-08
镁川(广州)绿色食品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, most of the related technologies of Cordyceps cicadae that have been disclosed are some simple and direct treatment methods, such as directly extracting its main active ingredients from raw materials, directly using them as an added component in a traditional Chinese medicine composition, etc., which are rare. It is difficult to maximize the self-efficacy of Cordyceps cicadae with the technology aimed at the ability of Cordyceps to scavenge free radicals

Method used

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  • Preparation method and application of isaria cicadae extracting solution
  • Preparation method and application of isaria cicadae extracting solution
  • Preparation method and application of isaria cicadae extracting solution

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Weigh a certain amount of Cordyceps cicadae, crush it, and pass through a 60-mesh sieve to obtain Cordyceps cicadae powder, then add pure water according to the solid-to-liquid ratio of 1:5, then put it in an ultrasonic cleaner, heat it to 60°C, and the ultrasonic frequency is 30kHz, Ultrasonic extraction for 60 minutes, centrifugation at 4000 rpm / min for 10 minutes, and suction filtration with slow filter paper to obtain the liquid. Repeat the extraction with water for 3 times, and combine the extracts for use; add 5‰ of yeast extract and 1‰ of glucose, sterilize by high-pressure steam at 121°C for 20 minutes, and turn on the ozone machine for 20 minutes in advance in the aseptic operation room, take it to the aseptic operation table, and cool it down to room temperature, add 1‰ compound probiotics, ferment at 30°C for 20 hours, and finally obtain the extract.

Embodiment 2

[0034] Weigh a certain amount of Cordyceps cicadae, crush it, and pass through a 120-mesh sieve to obtain Cordyceps cicadae powder, then add pure water according to the solid-to-liquid ratio of 1:20, then put it in an ultrasonic cleaner, heat it to 100°C, and the ultrasonic frequency is 40kHz, Ultrasonic extraction for 60 minutes, centrifugation at 4000 rpm / min for 10 minutes, and suction filtration with slow filter paper to obtain the liquid. Repeat the extraction with water for 3 times, and combine the extracts for use; add 10‰ yeast extract and 5‰ glucose, sterilize with high-pressure steam at 121°C for 20 minutes, turn on the ozone machine for 20 minutes in advance in the aseptic operation room, take it to the aseptic operation table, and cool it down to room temperature, add 4‰ compound probiotics, ferment at 42°C for 40 hours, and finally obtain the extract.

Embodiment 3

[0036] Weigh a certain amount of Cordyceps cicadae, crush it, and pass through a 80-mesh sieve to obtain Cordyceps cicadae powder, then add pure water according to the solid-to-liquid ratio of 1:10, then put it in an ultrasonic cleaner, heat it to 80°C, and use an ultrasonic frequency of 40kHz. Ultrasonic extraction for 60 minutes, centrifugation at 4000 rpm / min for 10 minutes, and suction filtration with slow filter paper to obtain the liquid. Repeat the extraction with water for 3 times, and combine the extracts for use; add 10‰ yeast extract and 4‰ glucose, sterilize with high pressure steam at 121°C for 20 minutes, turn on the ozone machine for 20 minutes in advance in the aseptic operation room, take it to the aseptic operation table, and cool it down to room temperature, add 3‰ compound probiotics, ferment at 40°C for 30 hours, and finally obtain the extract.

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Abstract

The invention discloses a preparation method and application of an isaria cicadae extracting solution. The preparation method comprises the steps of extracting isaria cicadae powder liquid by an ultrasonic method and a heating method, and further fermenting the obtained isaria cicadae powder liquid through composite probiotics. An additional carbon source and a nitrogen source are added in the fermentation process so that better living environment can be provided for probiotics; and through determining the growth curve of the composite bacteria and monitoring the growth condition, the fermentation condition is further optimized, and the capacity of the obtained isaria cicadae extracting solution for eliminating DPPH free radicals can be effectively improved. Through the adoption of the preparation method disclosed by the invention, the utilization rate of isaria cicadae can be increased, and the obtained isaria cicadae extracting solution has better oxidation resisting effects than anoriginal extracting solution, and can be used for preparing oxidation-resistant health-care products and drugs.

Description

technical field [0001] The invention relates to a preparation method of an extract of Cordyceps cicadae, in particular to a method for improving the antioxidant activity of the extract of Cordyceps cicadae. Background technique [0002] Cordyceps cicadae, also known as chrysanthemum, golden cicada, big cicada, and Cordyceps sinensis and Cordyceps militaris belong to the kingdom of fungi, fungi, ascomycetes, ergot, ergot family, and genus Cordyceps. The nymph of the nymph is parasitized to death by the conidia of the Ergotaceae fungus Cicada grandis, and the subseat formed by long hyphae in its head is shaped like a flower bud, so it is called cicadae. Cordyceps cicadae is cold in nature and sweet in taste. It has the effects of dispersing wind and clearing heat, calming convulsions, clearing rashes, improving eyesight and reducing cloudiness. It is produced in Zhejiang, Sichuan, Yunnan, Jiangsu and other places. One of the traditional precious Chinese medicinal materials. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/068A61P39/06A23L33/00
CPCA61K36/068A61P39/06A23L33/00A61K2236/331A61K2236/53A61K2236/19A23V2400/137A23V2400/113A23V2400/249A23V2400/517A23V2400/519A23V2400/529A23V2400/531A23V2400/533A23V2400/169
Inventor 潘亚宏佘志刚梅秀丽李琳李闯彪
Owner 镁川(广州)绿色食品科技有限公司
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