101 results about "Cordyceps cicadae" patented technology

The invention discloses cordyceps cicadae wine and a preparation method thereof. The cordyceps cicadae wine is obtained by dipping coremium of paecilomyces cicadae and/or mycoplasma, as raw materials, in base wine, or obtained by mixing an extract of the coremium of paecilomyces cicadae and/or an extract of mycoplasma, as raw materials, with drinking wine. In the cordyceps cicadae wine, the raw materials are fully utilized, activated secondary metabolism components in the mycoplasma are effectively kept, the integrity of active components in the cordyceps cicadae wine is ensured, the product quality is stable and the production cost is low.

The present invention provides a traditional Chinese medicine health care product, its preparation method and its application. The traditional Chinese medicine health care product is prepared by taking Cordyceps cicadae from artificial cultivation as a main raw material. The traditional Chinese medicine health care product has the functions of improving anemia and sleep disorders. Animal experiments show that the traditional Chinese medicine health care product has the advantages of safety, effectiveness and no obvious side effect.

The invention relates to an active substance of cordyceps cicadae, a preparation method of the active substance, pharmaceutical composition containing the active substance and application of the active substance. The active substance of the cordyceps cicadae is used for preventing and/or treating xerophthalmia and can be prepared with a method comprising steps as follows: cordyceps cicadae mycelia are taken and cultured on a plate medium for 5-14 days at the temperature of 15-35 DEG C; the cultured cordyceps cicadae mycelia are inoculated into a flask and cultured for a few days under the conditions that the temperature is 15-35 DEG C and the pH is 2-8; the cultured cordyceps cicadae mycelia are inoculated into a fermentation tank and cultured for 3-5 days under the conditions that the temperature is 15-35 DEG C, the pH is 2-8, the tank pressure is 0.5-1.0 kg/cm<2> and the ventilation rate is 0.01-1.5 VVM, and cordyceps cicadae mycelium fermentation broth is formed; the fermentation broth is subjected to freeze drying and ground into powder, and cordyceps cicadae mycelium freeze-dried powder is formed; the freeze-dried powder is extracted with a solvent, and a cordyceps cicadae mycelium extract solution is formed; the extract solution is dried, and the active substance of the cordyceps cicadae is obtained. The active substance of the cordyceps cicadae can be used for preparing the pharmaceutical composition for preventing and/or treating xerophthalmia.

The invention relates to a cordyceps cicadae extract improving immunity, resisting tumor, improving renal function and promoting sleep, which effectively solves the problems that the cordyceps cicadae extract is low in content of effective components, low in extraction yield of the effective components, low in product yield and low in product activity. The extract is prepared by the following steps: crushing a cordyceps cicadae medicinal material; adding pure water; carrying out circulating ultrasonic treatment; carrying out centrifugal filtration and removing residues to obtain an extraction liquid; micro-filtering the extraction liquid by using an ultrafiltration membrane to obtain a clean liquid; concentrating the clean liquid by a membrane to a concentrated liquid with the weight ratio of 1.15-1.20; freezing; and drying. According to the extract provided by the invention, the effective components such as cordycepin, cordycepic acid (mannitol), adenosine and polysaccharide are extracted at a relatively low temperature, the extraction yield is high and the product yield is high; the whole production line at a low temperature not only prevents the active components in the product from being degraded, but also reduces the loss of flavor of cordyceps cicadae; the reconstitution property of the product is further improved by virtue of a freezing and drying technique, and the instant solubility of the product is jointly guaranteed by the freezing and drying technique and the microfiltration technique.

The invention discloses a health-care medicine composition. The composition is prepared from, by mass, 60-600 parts of glutathione, 50-300 parts of silymarin, 1-100 parts of auricled hedyotis herb extracts, 1-100 parts of potentilla disclor bunge extracts, 1-100 parts of cordyceps cicadae extracts and auxiliary materials. The composition comprises, by mass, one or more of 50-200 parts of vitamin C, 50-200 parts of vitamin E, 100-300 parts of astaxanthin, 100-300 parts of lycopene, 100-300 parts of xanthophylls, 100-300 parts of beta-carotene, 100-300 parts of grape seed extracts, 100-300 parts of anthocyanin, 100-300 parts of ganoderma spore powder, 200-1000 of coenzyme Q10 and 200-1500 parts of garlic essence oil. The composition has the functions of clearing away toxin, treating radiation diseases, carrying out radiation protection, protecting the liver, enhancing immunity, improving the disease course and symptoms of some diseases, resisting allergy, beautifying faces, nourishing skin, improving eyesight, treating eye diseases, resisting aging and the like.

A method for reducing the toxicity of strychnos nux-vomica seeds by solid fermentation with medicinal fungi comprises the following steps of: activating and culturing schizophyllum commune, shiraia bambusicola, coriolus unicolor, cantharellus cibarius, beauveria bassiana and cordyceps cicadae for fermentation; oven-drying strychnos nux-vomica seeds, pulverizing, soaking in water, and autoclaving; respectively inoculating the above fungi on the treated strychnos nux-vomica seeds, performing solid fermentation under certain conditions, collecting respective fermentation product, and drying at low temperature to obtain medicinal fungal substances. The inventive method is simple and reasonable, and can obtain six medicinal fungal substances with reduced toxicity of strychnos nux-vomica seeds suitable for treatment purpose. The medicinal fungal substances have toxicity remarkably lower than that of strychnos nux-vomica seeds, have definite analgesic and antiphlogistic effects, and can keep the original main drug effect of Strychnos nux-vomica seed.

A method of preparing Cordyceps cicadae mycelium active substances for preventing and/or treating xerophthalmia is provided. The method comprises: (a)culturing a Cordyceps cicadae mycelium in a plate media at 15 to 35° C. for 5 to 14 days; (b)inoculating the mycelium of step(a) to a flask containing liquid media and culturing it at 15 to 35° C. with a pH of 2 to 8 for 3 days; (c)inoculating the mycelium of step(b) to a fermenter tank and culturing it at 15 to 35° C. with a pH of 2 to 8 for 3 days, so as to obtain a Cordyceps cicadae mycelium fermentation liquid; (d)freeze-drying and grating the fermentation liquid, so as to obtain a Cordyceps cicadae mycelium powder; (e)extracting the powder with solvent, so as to obtain a Cordyceps cicadae mycelium extract; and (f)drying the extract, so as to obtained the Cordyceps cicadae mycelium active substances.

The invention discloses a medium inoculation technology and its special equipment in the Cordyceps cicadae solid fermentation industry. Specifically, the technology includes the steps of: 1. putting wheat in a wheat cleaning machine for cleaning; 2. conveying the cleaned wheat to an integrated tank top through a hoisting instrument, and making the wheat enter the integrated tank through a wheat inlet at the integrated tank top, and under a temperature of 121DEG C, conducting stewing and sterilization for 45 minutes; and 3. cooling the wheat to 30DEG C through sterile air and a cooling device of the integrated tank, introducing a bacterial solution into the integrated tank, which rotates around a shaft to make the wheat and the bacterial solution fully and uniformly mixed. The invention has the beneficial effects that: 1) automation of wheat cleaning, conveying and inoculation is realized, and heavy physical work is reduced; and 2) cooling is performed through a jacket and an in-tank mode, the sterile air is input into the tank to contact a material so as to bring heat out of the tank body, a cooling solution is input into the jacket so as to make the material at 121DEG C cooled to 30DEG C within a short period of time, and the material is ensured good fluidity.

The invention discloses cordyceps cicadae/cordyceps sobolifera mycelium active substance and a composition for protecting nerve cells. Under the noncytotoxic concentration of 40 mu/ml, the cordyceps cicadae/cordyceps sobolifera mycelium active substance can be used for protecting the neurotoxicity of MPP+ to the dopaminergic neuron, and also can be used for inhibiting the cell apoptosis caused by the pressure of the endoplasmic reticulum caused by tunicamycin, and therefore, the cordyceps cicadae/cordyceps sobolifera mycelium active substance has the potential of preventing neurodegenerative diseases.

The invention relates to a Cordyceps cicadae extract and application thereof in preparing nerve protecting and anti-aging medicaments. The extract is a Cordyceps cicadae ethanol extraction water soluble part and is prepared by the steps of pulverizing Cordyceps cicadae, sieving, performing reflux extraction with ethanol, dispersing ethanol extract in water, performing gradient extraction with petroleum ether, ethyl acetate and n-butanol in sequence, concentrating under reduced pressure to recover solvent, freeze-drying to obtain a petroleum ether part, an ethyl acetate parts, a n-butanol part and a water part, and concentrating the water part; separating with AB-8 macroporous adsorption resin, washing with water to nearly colorless, and eluting with 80% ethanol; collecting ethanol eluate, concentrating, and freeze-drying to obtain water soluble extract (JCHH2O). The extract provided by the invention can remarkably inhibit glutamic acid-induced aging injury of PC12 cells, and reduce intracellular oxygen radical level, and has in vitro antioxidant activity in experiments of scavenging DPPH. and superoxide anion (O<2.->) free radicals.

The invention discloses a liquid fermentation culture medium formula capable of improving the biomass of cordyceps cicadae fungi and the content of an active ingredient. Water is used as a solvent and a culture medium is prepared from: 30g/L to 40g/L of sucrose, 15g/L to 25g/L of peptone, 0.5g/L to 1.5g/L of KH2PO4 and 0.5g/L to 1g/L of MgSO4 7H2O. A fermentation culture medium is subjected to design optimization, and an optimal carbon source, nitrogen source and inorganic salt and corresponding concentrations are obtained; the biomass of the cordyceps cicadae fungi and the content of the active ingredient, namely cordycepic acid, can be greatly improved.

The invention discloses a cordyceps cicadae whitening and moisturizing cosmeceutical.The cosmeceutical is prepared from, by mass, 8% of cordyceps cicadae extracting solution, 6% of natural plant oil, 0.05-0.1% of chelating agent, 3% of moisturizing agent, 5% of co-emulsifier, 0.5% of thickener, 0-1% of fragrance modifier, 5.0% of glycerinum and the balance deionized water.The invention further discloses a preparing method of the cordyceps cicadae whitening and moisturizing cosmeceutical.By the adoption of the method, effective constituents of cordyceps cicadae can be reserved as much as possible, and the value of cordyceps cicadae can be fully realized.

The invention belongs to the technical field of extraction and separation, in particular discloses a method for extracting peroxy ergosterol from cordyceps cicadae. The method comprises the steps of preparation of crude extract of peroxy ergosterol by an ultrasonic method, enrichment with a lipophilic solvent, separation and purification with a macroporous resin, crystallization and purification and the like. The method adopts ultrasonic extraction, has the advantages of low extraction temperature, short extraction time, high efficiency and the like, the extraction efficiency of peroxy ergosterol is improved, the method also adopts the the macroporous resin which has a good adsorption effect on peroxy ergosterol, and the yield of peroxy ergosterol is increased. The method is simple to operate, the prepared peroxy ergosterol has high purity and high yield, the process stability is good, the production cost is low, the method is suitable for scale production, and has a better applicationprospect.

The invention provides an active substance of cordyceps cicadae/sobolifera and the use thereof for reducing intraocular pressure. The active substance of cordyceps cicadae/sobolifera is prepared by the steps of (a) culturing the cordyceps cicadae/sobolifera mycelium on a flat plate culture medium at 15 to 35 DEG C for 5 to 14 days; (b) inoculating the cordyceps cicadae/sobolifera mycelium culturedin the step (a) into a flask to be cultured at 15 to 35 DEG C and pH 2 to 8 for 3 to 10 days; (c) inoculating the cordyceps cicadae/sobolifera mycelium cultured in the step (b) into a fermentation tank to be cultured at the conditions of 15 to 35 DEG C and pH 2 to 8 for 3 to 10 days to form a cordyceps cicadae/sobolifera mycelium fermentation liquid; (d) freeze-drying the cordyceps cicadae/sobolifera mycelium fermentation liquid, then grinding into powder to form a cordyceps cicadae/sobolifera mycelium freeze-dried powder; and (e) performing solvent extraction on the cordyceps cicadae/sobolifera mycelium freeze-dried powder to form a cordyceps cicadae/sobolifera mycelium extract containing the active substance of cordyceps cicadae/sobolifera.

The invention discloses a Taisui fermented Chinese herbal medicine preparation for treating blood glucose and a preparation method, and belongs to the technical field of bioengineering and fermentation. The preparation takes cyclocarya paliurus, green Bonnie flower, radix salviae miltiorrhizae, fiveleaf gynostemma herb, eucommia bark, radix astragali, dendrobii officinalis caulis and cordyceps cicadae powder as main raw materials, and is supplemented by a sugar source and water; and scientifically cultured Taisui stock solution compound strains are used, inoculation amount is 20-30%, culture is performed in a fermentation tank for 10-15 days, pH is 5-7.5, and temperature is 26 DEG C. Through complete fermentation, the medicinal ingredients in Chinese herbal medicines can be maximally released, and enzymes and lots of active materials can be generated; and in addition, Taisui can release active materials such as PQQ, nucleotide, chitin, flavone, polysaccharide, glutathione and saponin, which have obvious treatment effects on metabolic diseases of diabetes and hyperglycemia.

The invention provides a solid culture method of cordyceps cicadae. The culture method is characterized in that according to the method, cordyceps cicada strains subjected to expanded culture are inoculated onto a solid culture medium, dark culture and light culture are carried out in sequence, and fruiting bodies are harvested timely, so that the cordyceps cicadae is obtained, wherein the illumination of 16-20 hours per day is adopted in the light culture stage.

The invention relates to use of cordyceps cicadae in preparation of drugs for preventing or treating gout. According to the use, due to cordyceps cicada preparation products, a novel gout preventing or treating method is provided and is obvious in effect. The cordyceps cicadae have the functions of reducing blood and urine creatinine, increasing endogenous creatinine clearance rate, increasing the content of serum protein, reducing the discharge of urinary protein, and the like. Therefore, the cordyceps cicadae have definite treatment effect on early- and middle-stage chronic renal insufficiency patients. Confirmed by further researches: the cordyceps cicadae have relatively good treatment effect on renal tubulointerstitial lesions and can be used for protecting Na<+>-K<+>-ATPase of renal tubule cells, alleviating cytolysosome and cell lipid peroxidation injury, improving renal hemodynamics and alleviating endothelial cell injury and blood coagulation property.

The invention relates to the field of foods, in particular to a healthcare food for enhancing immunity and lowering blood sugar. The healthcare food is characterized in that active ingredients of the healthcare food are composed of the following raw materials in parts by weight: 20-40 parts of cordyceps cicadae fruiting body, 10-30 parts of radix ginseng tablets, 10-30 parts of radix salviae miltiorrhizae tablets, 10-30 parts of lentinula edodes and 5-15 parts of green tea. The healthcare food provided by the invention has effects of enhancing immunity and lowering blood sugar. Another purpose of the invention is to provide a preparation method of the healthcare food for enhancing immunity and lowering blood sugar, wherein the preparation process is simple.

The invention provides ointment for treating insomnia as well as a preparation method and an application method thereof and belongs to the pharmaceutical industry. The ointment provided by the invention is prepared from the following raw materials: herba cordyceps cicadae powder, poria with hostwood, radix angelicae sinensis, white paeony root, radix bupleuri, fried bighead atractylodes rhizome, cortex albiziae, semen ziziphi spinosae, semen platycladi, radix polygalae, longan pulp, semen nelumbinis, lily bulbs, fructus schisandrae chinensis, radix ophiopogonis, plum blossom, rhizoma acori tatarinowii, radix curcumae, radix acanthopanacis senticosi, rhizoma polygonati, herba hyperici perforati, rhizoma nardostachyos, ganoderma lucidum, radix et rhizoma salviae miltiorrhizae, fructus tritici levis, fructus citrus sarcodactylis, tangerine peel, fructus crataegi, fructus jujubae, prepared liquorice root, (decocted firstly) mother-of-pearl and (decocted firstly) raw dragon bone. The ointment provided by the invention has the characteristics of good curative effect, high healing rate, safety to use, no toxic and side effects, convenience for carrying and easiness in preservation.

The invention discloses a cordyceps cicadae polysaccharide, a preparation method and application thereof in preparation of a medicament for preventing renal interstitial fibrosis. The method comprisesthe following steps: pulverizing cordyceps cicadae fruiting bodies, and performing sieving to obtain cordyceps cicadae fruiting body powder; ultrasonically extracting the fruiting body powder with distilled water, and then carrying out ultrahigh pressure treatment; preparing crude polysaccharide of the cordyceps cicadae fruiting body after the treatment; and then performing separation by using DEAE-cellulose anion column chromatography, and performing freeze-drying to obtain the purified polysaccharide. The method successfully prepares the cicada cordyceps polysaccharide treated and modifiedby ultrahigh pressure, and has the advantages of simple steps and higher efficiency. The intervention on a rat glomerular epithelial cell model shows that the polysaccharide can effectively inhibit the proliferation of rat glomerular epithelial cells (HMC), and has wide application prospect in the aspect of preventing renal interstitial fibrosis. The oxidation resistance of the cordyceps cicadae polysaccharide is obviously improved, and the cordyceps cicadae polysaccharide induces the apoptosis of high-glucose rat glomerular mesangial cells, has no toxicity to normal cells, and has good research value and commercial value.