Active substance of cordyceps cicadae, preparation method of active substance, pharmaceutical composition containing active substance and application of active substance

An active substance, a technology of cicada flower, which is applied in the development field of cicada flower active substances, and can solve the problems of corneal damage or dry eye symptoms prevention use and effect without a clear preparation method.

Active Publication Date: 2017-01-04
GRAPE KING
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both of the above two cases are compound applications with the addition of cicadae fruiting bodies. The clinical observations of intraocular pressure and vernal conjunctivitis respectively do not cle

Method used

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  • Active substance of cordyceps cicadae, preparation method of active substance, pharmaceutical composition containing active substance and application of active substance
  • Active substance of cordyceps cicadae, preparation method of active substance, pharmaceutical composition containing active substance and application of active substance
  • Active substance of cordyceps cicadae, preparation method of active substance, pharmaceutical composition containing active substance and application of active substance

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0077] Preparation of Active Substances of Cicada Flower

[0078] Cicada mycelium source

[0079] The used cicadae (Cordyceps cicadae) mycelium in the embodiment of the present invention is collected from the wild cicadae fruiting bodies in Taiwan, China, and its mycelium is obtained through separation, and it is preserved on a plate culture medium for generations. The identification by the Industrial Development Research Institute confirmed that its gene sequence is Cordyceps cicadae, and this strain has been publicly deposited in the Bioresource Research Center (BCRC) of the Food Industry Development Research Institute of the Foundation, with the registration number MU30106. This strain was also deposited on May 4, 2015 at the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures. The address of the preservation unit is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sc...

Embodiment 1

[0096] Embodiment 1 Cicada flower liquid state fermentation culture and the preparation of active substance

[0097] Mycelium strains: plate culture: the Cicada mycelium was inoculated on a plate medium, and the medium was potato dextrin medium (Potato Dextrose Agar, PDA), and cultivated at 25° C. for about 5 days.

[0098] Flask culture:

[0099] Scrape the hyphae on the plate and inoculate into the flask, use the medium in Table 2 below, at about 25°C, pH 4.5, shake and culture on a shaker at a speed of 120rpm for 3 days;

[0100] Table 2. Culture medium formula

[0101]

[0102] Fermentation tank culture:

[0103] The culture medium is the same as in Table 2. Inoculate the flask culture into the culture medium of the fermenter, at 25°C, the tank pressure is 0.5-1.0kg / cm 2 , pH 4.5, 10-150rpm stirring speed or no stirring (air lift) situation, feed air with 0.5-1.0VVM aeration rate, cultivate for 3 days, get mycelium and clarified liquid, called cicadae mycelium fermen...

Embodiment 2

[0113] Example 2 Analysis of Dry Eye Animal Model and Related Indicators

[0114] 1. Establishment of dry eye mouse animal model

[0115] Experimental animals:

[0116] Strain ICR female mice were purchased from Lesco Company (Taiwan, China), aged 7-10 weeks, weighing 25-33 grams, and were raised in the Experimental Animal Center of Sun Yat-sen Medical University, provided with normal clean feed and drinking water, and kept under 12 hours of light And 12 hours of dark cycle light, the temperature is controlled at 20±2°C, and the humidity is controlled at 50±5%.

[0117] (1) Ultraviolet light UVB induced dry eye syndrome

[0118] The ultraviolet lamp was purchased from Vilber lourmat Company, the lamp tube model was VL-6MC, the wavelength was set in the range of 280nm-320nm, and the main peak was at 312nm.

[0119] Before the test, the mice were randomly divided into 4 groups, 6 in each group, which were respectively the control group (fed with saline, not fed with cicadae a...

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Abstract

The invention relates to an active substance of cordyceps cicadae, a preparation method of the active substance, pharmaceutical composition containing the active substance and application of the active substance. The active substance of the cordyceps cicadae is used for preventing and/or treating xerophthalmia and can be prepared with a method comprising steps as follows: cordyceps cicadae mycelia are taken and cultured on a plate medium for 5-14 days at the temperature of 15-35 DEG C; the cultured cordyceps cicadae mycelia are inoculated into a flask and cultured for a few days under the conditions that the temperature is 15-35 DEG C and the pH is 2-8; the cultured cordyceps cicadae mycelia are inoculated into a fermentation tank and cultured for 3-5 days under the conditions that the temperature is 15-35 DEG C, the pH is 2-8, the tank pressure is 0.5-1.0 kg/cm<2> and the ventilation rate is 0.01-1.5 VVM, and cordyceps cicadae mycelium fermentation broth is formed; the fermentation broth is subjected to freeze drying and ground into powder, and cordyceps cicadae mycelium freeze-dried powder is formed; the freeze-dried powder is extracted with a solvent, and a cordyceps cicadae mycelium extract solution is formed; the extract solution is dried, and the active substance of the cordyceps cicadae is obtained. The active substance of the cordyceps cicadae can be used for preparing the pharmaceutical composition for preventing and/or treating xerophthalmia.

Description

technical field [0001] The invention relates to the development of active substances of cicadae, in particular to the technical field of preventing dry eye-related lesions induced by physical or chemical damage. Background technique [0002] Xerophthalmia [0003] "Dry eye syndrome" is one of the diseases that are quite common in ophthalmology clinics (accounting for 10-15% of the adult population). It is mainly caused by the lack of tears on the surface of the eyeball or poor tear quality. Common symptoms include dry eyes and easy tiredness , drowsiness, itching, foreign body sensation, burning sensation, tight and heavy eyelids, sticky secretions, fear of wind, photophobia, sensitivity to external stimuli, and temporary blurred vision; sometimes the eyes are too dry and the basic tears are insufficient Stimulate reflex tear secretion and cause frequent tearing symptoms; more severe patients, eyes will be red, swollen, hyperemic, keratinized, corneal epithelium is broken a...

Claims

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Application Information

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IPC IPC(8): A61K36/066A61P27/02A61K35/64
Inventor 林培正张菡馨陈劲初叶淑幸李丽雅徐瑞霞
Owner GRAPE KING
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