74results about How to "Protect wild resources" patented technology

A method for reproduction of morel includes such steps as choosing proper field in forest or others, removing harmful plants, sowing, conventional management, and picking up. Its advantages are high yield and high quality.

The invention discloses an ancherythroculter nigrocauda artificial breeding method comprising the steps of (1) parent fish breeding, (2) artificial spawning induction, (3) artificial insemination, (4) artificial incubation and (5) fry culturing. The method is simple, and has high operability and good practicality. With the method, ancherythroculter nigrocauda fry scale expansion and industrialized production can be realized, ancherythroculter nigrocauda aquaculture development can be promoted, and ancherythroculter nigrocauda wildlife resource can be effectively protected. As a result of tests, with the method, an ancherythroculter nigrocauda induced spawning rate is 80-90%, an ancherythroculter nigrocauda fertilization rate is 60-90%, and an ancherythroculter nigrocauda hatching rate is 70-90%.

The invention discloses a method of propagation by cuttings for dracaena cochinchinensis SC chen, which consists of selecting branches of dracaena cochinchinensis SC chen trees with an age of over two years, cutting the upper end of the branches, cutting the lower end to 40-50 degree of inclined surface, sealing the upper extreme tangent plane of the grafting ear with paraffin wax, immersing the lower end tangent plane in aqueous solution of root-producing powder, carrying out cuttage by the conventional process and transferring into large field planting.

The invention relates to an artificial cuttage breeding method of wild Rosa odorata var. gigantea. The method comprises the following steps of: selecting a wild Rosa odorata var. gigantea tree which grows for 3-5 years and has favorable development of a tiller branch of the root and stem part as a female parent material; cutting the lignified or semi-lignified raw branch in the current year, which is the stem and root part and is not budded, as a fringe; selecting the tip part and middle section part of the fringe for manufacturing inserted fringe; carrying out right-angle cutting on an upper notch in the cutting position, wherein a lower notch is an inserted fringe base notch; carrying out slope cutting and cleaning disinfection on the node and soaking the node in a root growing powder accelerating agent aqueous solution, and then inserting into a seedling bed according to the plant row space of 30cm*10cm; and after growing and budding the inserted fringe, withdrawing a sunshade screen and entering a natural growth phase. The method disclosed by the invention is simple and convenient and has low cost; on one hand, the method provides sufficient raw material guarantee for the production of a pharmaceutical enterprise and realizes the scientific utilization of plant resources; on the other hand, the method protects wild resources and ecological environment and has favorable market application prospect.

The invention discloses a seedling method for paris polyphylla. A large number of complete plants can be obtained by breaking dormancy of latent buds at a low temperature and then carrying out shoot-inducing and root-inducing treatment, so that large-scale industrial breeding of the paris polyphylla is achieved. According to the seedling method, the inhibiting effect of various latent buds on a single rootstock of the paris polyphylla is completely avoided; the propagation coefficient is increased to the maximal extent; the hereditary stability of the paris polyphylla variety is fully ensured; and meanwhile, the phenomenon that variation of seeds and tissue culture seedlings is easily generated is avoided. Compared with other propagation methods employing the rootstocks, the seedling method has the advantages that completely separate plants can be formed; and seedlings with a plurality of buds can also be formed.

Disclosed is a method for breeding scorpions. Domesticated buthus martensii karsches serve as female parents and wild eastern Asian king scorpions serve as male parents to be hybridized, and litters reproduced by pregnant scorpions are first-generation eastern Asian king scorpions. The first-generation eastern Asian king scorpions serve as female parents, the wild eastern Asian king scorpions serve as male parents to be hybridized, and litters reproduced by pregnant scorpions are second-generation eastern Asian king scorpions. The second-generation eastern Asian king scorpions serve as female parents, the wild eastern Asian king scorpions serve as male parents to be hybridized, and litters reproduced by pregnant scorpions are third-generation eastern Asian king scorpions. The wild eastern Asian king scorpions serving as male parents or female parents before hybridization can be selected, and individuals which are strong and yellow in gourd, yellowish and bright in tails, bulging and round in forelimb pincers, quick in action and sexually mature are selected. The method for breeding scorpions has the advantages of little investment, rapid effect taking, small labor intensity and no pollution. Both wild resources are protected, and requirements of domestic and foreign markets can be met.

The invention provides a culture method of glycyrrhiza hairy roots and a method for preparing glycyrrhiza total flavonoids from glycyrrhiza hairy roots and culture solution thereof. The method comprises the following steps: culturing glycyrrhiza hairy roots in a fluid nutrient medium which does not contain Tween to the growth peak period; culturing the obtained glycyrrhiza hairy roots in the growth peak period in a fluid nutrient medium which contains Tween for more than 5 days; and eluting by ethanol with different concentration through a polyamide chromatography column, and separating the total flavonoids from the culture solution, wherein the transfer rate of the glycyrrhiza total flavonoids is 97.23%, the transfer rate of glycyrrhiza chalcone A is 98.14%, and the transfer rate of Glabridin is 97.54%. The invention also provides the glycyrrhiza total flavonoids prepared by the method and medical applications of the glycyrrhiza total flavonoids.

The invention discloses a method for promoting seeds of meconopsis integrifolia to germinate. The method comprises the steps of firstly drying the seeds of the meconopsis integrifolia for 10-15 hours at 30-40 DEG C; then putting the dried seeds in darkness for 24-48 hours at the conditions that the temperature is -15--10 DEG C and the humidity is 70-80%; putting the seeds into a polyethylene glycol solution with the mass concentration being 12-16%, keeping at 2-6 DEG C, and soaking for 15-24 hours in darkness; soaking for 2-4 hours in 10wt% ammonia water, and washing the surfaces of the seeds by distilled water; soaking the seeds in 50-400mg / L gibberellin solution for treating for 12-18 hours, and washing the surfaces of the seeds by the distilled water; finally, germinating in the darkness under the conditions that the temperature is 5-10 DEG C and the humidity is 80-90%. According to the method, the germination rate of the seeds reaches over 87% on the 20th day.

The invention relates to a method for propagating boletus edulis, belonging to the technical field of edible fungus. The technical scheme of the method comprises the following steps: selecting a propagating land, preparing a liquid strain, seeding the strain, maintaining and managing the forest land and collecting the boletus edulis. The method fills the gap that sporocarp is not cultivated successfully artificially; artificial strains of boletus are spread in a natural forest with corresponding technical measures under a proper habitat condition, and promoted to propagate in natural condition, so that the natural yield and quality of the boletus edulis can be improved, the natural yield of the boletus edulis can be remarkably improved, and preferable economical benefit can be provided while breakthrough is generated in technology.

The invention relates to a method for cultivating chrysanthemum potato, wherein it comprises inserting branches, shearing graft, and immerging, potato and managing seed bed. The invention can grow 100thousand chrysanthemum potato; after three year transplantation, each unit can grow 6000kg, the content of saponin can reach 3-4%.

The invention provides a method for domesticating the wild Lhasa schizophrenia. Including collection of broodstock, transportation of broodstock, pre-treatment of temporary breeding, temporary breeding of broodstock and domestication of broodstock, the domestication method is simple, highly operable and practical, and can realize the large-scale and standardized domestication of wild Lhasa scutellaria, Promoting the development of Lhasa's scutella aquaculture industry and effectively protecting the wild resources of Lhasa's scutellaria.

The invention discloses an exogenous additive culture medium for promoting differentiation of anoectochilus formosanus protocorm. The culture medium is composed of a basic culture medium body and an exogenous additive, and by means of the culture medium, differentiation of the anoectochilus formosanus protocorm into seedlings can be obviously promoted. By means of the exogenous additive culture medium, tissue culture seedlings can be provided for large-scale planting of anoectochilus formosanus, and it is beneficial to protect anoectochilus formosanus wild resources.

The invention discloses an induction method and application of dryopteris fragrans callus tissues, and belongs to the technical field of biology. The method provided by the invention comprises the following steps: preparing dryopteris fragrans spores into a sterile spore suspension, inoculating the sterile spore suspension to improved Knop' s culture mediums, performing dark treatment, and after germination, performing culturing so as to obtain sporophytes; using stem tips and young and tender leaf bases of the obtained dryopteris fragrans sporophytes as explants, inoculating the explants to culture mediums in which 6-BA and NAA are added, and performing culturing so as to obtain green spherules; inoculating stem tips of the green spherules or the stem tips of the sporophytes into culture mediums containing 6-BA and 2,4-D, and performing proliferation and differentiation so as to obtain the callus tissues. The method disclosed by the invention lays the theory and practice foundation for establishing a rapid propagation system of dryopteris fragrans, so that wild resources of the dryopteris fragrans are protected, and the blank of performing callus tissue induction through stem tips and young and tender leaf bases in the tissue culture research of the dryopteris fragrans is filled.

The invention discloses a sebastodes fuscescens parent fish warming cultivation and childbearing method which comprises the following steps of: 1) selection of parent fish; 2) selection of farming facilities; 3) warming cultivation of the parent fish; and 4) childbearing. The method disclosed by the invention makes full use of the water quality conditions of the natural water body, the farming operation is simple and convenient, and the energy consumption is reduced. By adjusting the farming water temperature, the childbearing period of the sebastodes fuscescens parent fish is reduced by at least 20 days than the traditional method. Moreover, the parent fish obtained by the method has high quality, the parent fish can be recycled, and the normal childbearing rate can exceed 95%, thus the wild resources are protected while laying a foundation for obtaining high-quality child fish. The child fish obtained by the method is healthy with high quality and high survival rate, and large-specification fingerlings can be supplied in batches in advance; and moreover, by artificially controlling the environmental factor of indoor farming, the indoor artificial propagation method of sebastodes fuscescens is innovated, so that the parent fish gives birth in advance and an effective method is provided for obtaining large-scale fingerlings in advance.

The invention discloses an artificial domestication cultivation method for wild chanterelle, and relates to the technical field of edible mushroom cultivation. The wild chanterelle serving as a strain is obtained by strain separation and artificial domestication cultivation of stock cultures and cultivated species, so that wild resources of the species are protected, the supply of the market species of rare chanterelle is enriched, and higher economical and social benefits are achieved; meanwhile, the artificial domestication cultivation method is simple, low in pollution, pure in strain and easy to operate; by the adoption of a unique bagged material cultivation method, the cultivation period is shortened; the obtained stain is inoculated to cultivation mycelium, so that the germination rate is 82.5 percent, which is higher than that of the conventional method (56.5 percent) by 26 percent; therefore, the artificial domestication cultivation method is worth to be popularized and applied industrially.

The invention belongs to the technical field of artificial cultivation of rare medical fungi and relates to a method for culturing cordyceps militaris by taking living tenebrio molitor larvae as hosts. According to the method, the living tenebrio molitor larvae are taken as the hosts, inoculation of cordyceps militaris liquid is performed by artificial injection, and the contaminated living tenebrio molitor larvae are ossified slowly along with growth of in-vivo hyphae to grow out cordyceps militaris fruiting bodies through culturing. By adoption of the method, the contamination rate of the tenebrio molitor larvae reaches 85% or above, the ossification rate reaches 80% or above, and the cordyceps militaris is rich in content of effective components including cordyceps polysaccharide, cordycepin and cordycepic acid. The method is simple in operating process, high in yield and low in cost, cultured products are rich in nutrition and high in medicinal value, and suitableness for large-scale cultivation and a promising application prospect are realized.

The invention discloses a method increasing Paris polyphylla var yunnanensis maturing rate. The method comprises the following steps of (1) selecting strong and free of insect diseases paris polyphylla var yunnanensis plants, collecting pollen from most plants with cracked pollen sacs after the plants blossoms, (2) utilizing a portable dust collector to collect thoroughly-grown and rich pollen groups to a polypropylene filtration film with the diameter of 10mm and an aperture of 450nm, (3) conducting hand-pollinating three days after most pollen sacs crack, dipping the collected pollen via a writing brush or a soft brush and then evenly coating the pollen on the Paris polyphylla var yunnanensis necking head. Each necking head is dipped for two times; hand-pollination can be conducted from 4pm to 8pm and finished on the day; the next day, the same operations are conducted; and hand-pollination is conducted once more. Paris polyphylla var yunnanensis maturing rate can be improved by more than 40%, so seed source is enlarged for agricultural production and higher promotion value can be achieved.

The invention provides a preparation method and culture medium of a saussurea medusa high yielding flavone cell line. The method comprises the following steps: inducing callus tissue through a saussurea medusa seed; performing solid successive transfer culture on obtained callus tissue; directly selecting a cell line with high total flavone content in the callus tissue subjected to successive transfer culture through a visual method; performing liquid culture, performing solid culture from liquid culture, and continuously performing repeated screening and subculture, so as to obtain a high yielding cell line which is rapid in development and high in total flavone content. The cell line and the culture medium obtained through the method have the advantages that the cell growth rate can reach 45g to 50g dry weight / liter / month when the lighting suspension culture temperature is about 25 DEG C, and the content of flavonoid can reach 12 percent of the dry weight of culture.

The invention provides a ginseng dormancy-free facility cultivation method. The method mainly includes following steps: soaking rhizome of ginseng in a gibberellin solution for 2-6h; cultivating a ginseng plant in a matrix; supplementing light for 7-12h each day, controlling room temperature to higher than 18 DEG C, and harvesting after maturing. The cultivation method is simple and convenient to operate, can be realized without professional personnel and is comparatively mild in operation condition; facility cultivation technology is adopted for dormancy-free cultivation of ginseng, so that growing period of ginseng is shortened greatly; ginseng of two years old can be harvested after going through facility cultivation for 2-3h, and yield of ginseng of two years old is equivalent to that of ginseng of 4-6 years old after going through common facility cultivation; an operation route which is more novel is provided for the ginseng cultivation method, and the cultivation method is worthy of large-scale popularization and application and fills related technical vacancy.

The invention discloses a method for manually cultivating silkworm chrysalis cordyceps cicadae by using silkworm chrysalis of domestic silkworms as hosts. The method comprises the following steps of separating and screening out cordyceps cicadae bacterial strains from wild cordyceps cicadae, inoculating the screened cordyceps cicadae bacterial strains to culture mediums, performing culturing until mycelium grows out, then performing light-shade alternate culturing, after spores overgrow, picking the spores in a washing manner, and making spore suspension; using in-vivo silkworm chrysalis of domestic silkworms as hosts, and enabling the in-vivo silkworm chrysalis of the domestic silkworms to be infected through cordyceps cicadae spore suspension by a method of injection, soaking or body spraying; and culturing the infected silkworm chrysalis of the domestic silkworms until the silkworm chrysalis is hardened, and then performing light-shade alternate culturing. According to the method disclosed by the invention, the in-vivo silkworm chrysalis is used as the hosts for cultivating the silkworm chrysalis cordyceps cicadae which is high in content of nutrient components and free from toxins, and the silkworm chrysalis cordyceps cicadae can be developed into various foods and health-care products, which are safe and reliable to eat. According to the method disclosed by the invention, the silkworm chrysalis of the domestic silkworms is used for cultivating the cordyceps cicadae, the culturing cycle is short, the technical technology is simple and convenient, and the method is suitable for large-scale production and has wide application prospects.

The poplar and phoenix cutting and seedling growing method includes collecting the same year twig as cutting shoot in the time from May to September, nourishing treatment of the cutting shoot, planting to wind sheltering and sunny seedling bed of slightly acid soil, covering with film to maintain humidity, and covering with shed for further growth of the seedling. The present invention obtains poplar and phoenix seedling with the excellent characteristic of the mother plant maintained for afforestation by means of asexual propagation.

The invention discloses a cultivation medium capable of conducting scale cultivation on cantharellus cibarius Fr and a method. The cultivation medium comprises a mother strain cultivation medium, a breeder strain, a cultivated strain cultivation medium, and cantharellus cibarius Fr compost, wherein the mother strain cultivation medium comprises water, dried cedar bark, dried oak wood bits, bran, potatoes, glucose, manganese sulfate and agar. The breeder strain and cultivated strain cultivation medium comprises weedtree wood bits, small-particle corn cobs, bran, cedar wood bits, land plaster and magnesium sulfate. The cantharellus cibarius Fr compost comprises weedtree wood bits, oak leaves, small-particle corn cobs, bran, cedar wood bits, land plaster, humus and magnesium sulfate. According to the cultivation medium capable of conducting scale cultivation of cantharellus cibarius Fr and the method, wild cantharellus cibarius Fr is subjected to tissue isolation, a mother strain is cultivated, and then is inoculated into the breeder strain, the breeder strain is inoculated into the cultivated strain cultivation medium to be cultivated into cultivated strain, and then the cultivated strain is sowed to the cantharellus cibarius Fr compost so that cantharellus cibarius Fr can be produced. The cultivation medium capable of conducting scale cultivation on cantharellus cibarius Fr is simple in technology, low in contamination rate, pure in strain, simple in operation, short in cultivation cycle, and the biological efficiency reaches 70%.

The invention relates to the field of traditional Chinese medicine, in particular to a traditional Chinese medicine composition for treating pain in the neck or the shoulders or the waist or the legs.The traditional Chinese medicine composition for treating pain in the neck or the shoulders or the waist or the legs is prepared from, by weight, 60-120 parts of scorpions, 110-160 parts of pig's nails, 10-35 parts of centipedes, 150-210 parts of rhizoma Chuanxiong, 40-90 parts of szechwan Chinaberry fruits, 40-90 parts of peach kernels, 30-80 parts of flos carthami and 45-80 parts of liquorice roots.The invention further relates to a preparation method of the traditional Chinese medicine composition.The traditional Chinese medicine composition is in scientific compatibility according to the traditional Chinese medicine theory, has the effects of dispelling wind and relieving pain, and activating blood and dredging collaterals, and has a good curative effect on patients suffering from pain in the neck or the shoulders or the waist or the legs.

The invention provides a mutagenic strain of cordyceps militaris and a breeding method. A new high-yield strain of cordyceps militaris is obtained by chemical mutagenesis, the preservation number thereof is CGMCC NO. 2909, and compared with original starting strain, the dry weight of mycelium is increased by 103%, the content of adenosine is increased by 536%, the content of polysaccharide is increased by 22.2%, the content of protein is increased by 55.5%, and the content of mannitol is increased by 37.5 %. The new strain of cordyceps militaris does not degenerate after more than 20 times of subculturing, has genetic stability, and utilizes the research of random amplified polymorphic DNA (RAPD) to verify the difference on DNA level between the strain and the original starting strain. The invention also discloses the optimum process condition for extracting polysaccharide and adenosine of cordyceps militaris.

The invention discloses an artificial cordyceps sobolifera cultivation method achieved by taking tussah silkworm chrysalises as hosts. The method comprises the following steps that cordyceps sobolifera strains are separated and screened from wild cordyceps sobolifera, a culture medium is inoculated with the cordyceps sobolifera strains, culturing is conducted until mycelia grow out, then light and shade alternate culturing is conducted, and after the culture medium is full of spores, the spores are washed down to be prepared into spore suspension; the living tussah silkworm chrysalises are taken as the hosts to be infected by the cordyceps sobolifera spore suspension through an injecting or soaking or body spraying method; the infected tussah silkworm chrysalises are cultured until the silkworm chrysalises become hard, and then light and shade alternate culturing is conducted. According to the method, the silkworm chrysalis cordyceps sobolifera is cultivated by adopting the living silkworm chrysalises as the hosts, rich in nutrition ingredient and free of toxicity, can be developed into various food and healthcare products and can achieve edible safety and reliability. The method for cultivating the cordyceps sobolifera through the tussah silkworm chrysalises has the advantages that the culture cycle is short, the technology is simple and convenient, the method is suitable for large-scale production, and the wide application prospect is achieved.

The invention discloses a hypericum perforatum seedling method which is implemented by the following steps: (A) selecting soil and a seeding manner, namely selecting a sandy soil land block rich in humus, wherein the broadcast sowing and row sowing depths are not more than 1 cm; (B) controlling the soil water content at different stages of seedling, namely controlling the soil water content from the sowing stage to the germination stage to be 50-60%, and the soil water content from the germination stage to the seedling stage to be 60-70%; (C) controlling tunnel temperature during seedling, namely controlling the average tunnel temperature between the sowing stage and the germination stage to be 32 DEG C; and (D) controlling weeds during seedling, namely not performing weed eradication from the sowing stage to the germination stage, and performing weed eradication when a seedling has 8-10 leaves and the root system is stable. The hypericum perforatum seedling method disclosed by the invention is short in seedling cycle and good in seedling growth vigor, root systems of cultured seedlings can reach 10 cm after 40 days, and the cultured seedlings have relatively more fibrous roots and are beneficial for field transplantation. The hypericum perforatum seedling method disclosed by the invention not only is beneficial for large-area planting culture and popularization of hypericum perforatum but also has great significance for protecting wild resources of the hypericum perforatum.

The invention relates to an interplanting method of anoectochilus roxburghii and polygonatum sibiricum under forests. The interplanting method comprises the following planting steps of selecting forests, performing land parcel treatment, performing land parcel soil improvement, selecting seedlings, performing planting, performing management after planting, and performing harvesting, wherein the step of selecting the seedlings comprises the steps of (1) soaking rhizome segment cutouts of the polygonatum sibiricum in a carbendazol bactericide solution prepared from diluting carbendazol bactericides with water of which the weight is 800-1500 times of that of the carbendazol bactericide, performing sealing with plant ash, and airing the treated polygonatum sibiricum rhizome segments as seed stems; (2) thoroughly cleaning roots of the anoectochilus roxburghii seedlings, performing disinfection with a 40-60% iprodione wettable powder solution prepared by diluting iprodione wettable powder with water of which the weight is 800-1500 times of that of the iprodione wettable powder, and airing the treated anoectochilus roxburghii seedlings as seedlings. According to the interplanting method of the anoectochilus roxburghii and the polygonatum sibiricum provided by the invention, the polygonatum sibiricum can just provide shady environment for the anoectochilus roxburghii, so that the landutilization rate can be increased, and soil, light and water resources can be fully utilized; and soft rot and stem rot of the anoectochilus roxburghii at the early stage can be alleviated, the planting survival rate of the anoectochilus roxburghii and the polygonatum sibiricum can be increased, and the quality of medicinal materials can be guaranteed.

This invention relates to a cultivation method for cells of Xuelian lilac aglycons including: applying Xuelian explant or seeds to bourgeon young plants to induce calli on the 1 / 2 cultivation medium, said callus cells are used to produce Xuelian lilac aglycons, MS cultivation medium is the solid for generating the Xuelian lilac aglycon cells or for suspending cultivation, 70% alcohol or n-butyl alcohol is used in separating, extracting and preparing the aglycons and HPLC is used for testing its content. This invented content of gross lilac compound is 3-4% of the dry weight of cells in this invented cell cultivating material and its productivity is 400-900mg / L.

The invention discloses a processing method of radix scrophulariae, and belongs to the technical field of processing of medicinal materials. The method comprises the following steps: firstly, cleaningcollected fresh roots of radix scrophulariae; simultaneously heating the fresh roots, and carrying out moisturizing treatment on the fresh roots; keeping the humidity of the fresh roots at 60%-100%,maintaining the state for 1-3 days, and stopping moisturizing of the fresh roots. According to the processing method, the quality of the medicinal materials is improved; the primary metabolism level of the radix scrophulariae is improved; the high-yield potential of the medicinal materials is fully excavated; the components are purely natural; the processing technique is designed according to theinfluence on the quality of the medicinal materials caused by adverse natural environment; the biosynthesis is promoted by the natural process of radix scrophulariae biosynthesis, and is different from a biotransformation technology, no reagent is needed to promote high expression of the secondary metabolism pathway, the components contained by the medicinal material of the radix scrophulariae arepurely natural; and no ingredient with toxic and side effects is generated.

The invention relates to the technical field of forest tree propagation, and in particular relates to a himalayan cypress germination accelerating composite and a preparation thereof and a seed sowing and seedling growing method of himalayan cypress. The himalayan cypress germination accelerating composite comprises IAA (Indole Acetic Acid), 6-BA (benayl aminopurine) and sodium nitroprusside. With the adoption of the himalayan cypress germination accelerating composite, the germination rate of himalayan cypress seeds can be obviously increased. According to the seed sowing and seedling growing method, the seed sowing and seedling growing are artificially performed through a greenhouse in a fine manner, so that a series of problems in a traditional seedling growing method can be overcome; the seedling rate is more than 85%; the uniformity is high; in addition, the seedlings grow well; the method has the characteristics that the seedling can grow quickly, the growing period is short, and the operation is simple; the problems in the himalayan cypress seed sowing and seedling growing process, such as low germination percentage of seeds, low seedling rate, weak seedlings, and seed sowing and seedling growing in spring, can be solved.