The invention discloses a cultivation medium capable of conducting scale cultivation on cantharellus cibarius Fr and a method. The cultivation medium comprises a mother strain cultivation medium, a breeder strain, a cultivated strain cultivation medium, and cantharellus cibarius Fr compost, wherein the mother strain cultivation medium comprises water, dried cedar bark, dried oak wood bits, bran, potatoes, glucose, manganese sulfate and agar. The breeder strain and cultivated strain cultivation medium comprises weedtree wood bits, small-particle corn cobs, bran, cedar wood bits, land plaster and magnesium sulfate. The cantharellus cibarius Fr compost comprises weedtree wood bits, oak leaves, small-particle corn cobs, bran, cedar wood bits, land plaster, humus and magnesium sulfate. According to the cultivation medium capable of conducting scale cultivation of cantharellus cibarius Fr and the method, wild cantharellus cibarius Fr is subjected to tissue isolation, a mother strain is cultivated, and then is inoculated into the breeder strain, the breeder strain is inoculated into the cultivated strain cultivation medium to be cultivated into cultivated strain, and then the cultivated strain is sowed to the cantharellus cibarius Fr compost so that cantharellus cibarius Fr can be produced. The cultivation medium capable of conducting scale cultivation on cantharellus cibarius Fr is simple in technology, low in contamination rate, pure in strain, simple in operation, short in cultivation cycle, and the biological efficiency reaches 70%.