5 results about "Hypericum perforatum" patented technology

This invention relates to the field of natural product separation technology, specifically to coumarin compounds isolated from *Hypericum perforatum*, their preparation methods, and applications. The preparation method of these coumarin compounds includes alcohol extraction from *Hypericum perforatum*, followed by macroporous resin adsorption separation and column separation. The coumarin compounds of this invention can be used for anti-inflammatory purposes, exhibiting inhibitory activity against RAW264.7 cells and the expression of TNF-α, IL-1β, and IL-6 proteins.

This invention discloses an optimized extraction and purification process for total flavonoids from Hypericum perforatum and its application in photodamage protection. Belonging to the field of natural product extraction technology, the process includes the following steps: using Hypericum perforatum powder as raw material, ultrasonic-assisted extraction is performed, followed by centrifugation, collection of the supernatant, and repeated extraction of the residue twice. The supernatants are combined to obtain a total flavonoid extract from Hypericum perforatum. The extract is then purified by adsorption using a macroporous adsorption resin, and the eluent is collected, concentrated, and dried to obtain high-purity total flavonoids from Hypericum perforatum. This invention features a stable process, high extraction efficiency, and is environmentally friendly, making it suitable for industrial production. The prepared total flavonoids from Hypericum perforatum exhibit high purity and significant antioxidant and photoprotective effects, and can be widely used in antioxidant, skin protection, and sunscreen products.

This invention relates to the field of plant-derived extracellular vesicles, and discloses a method for preparing and applying Hypericum perforatum-derived exosome-like nanovesicles (HPDENs). This invention prepares HPDENs with natural vesicle structures through a specific process, utilizing the natural characteristics of plant-derived vesicles to encapsulate Hypericum perforatum (…). HP The active ingredient, combined with nasal drops, achieves targeted delivery to the brain. It exerts its antidepressant effect by regulating microglia polarization imbalance and reshaping the "neuroimmune-synaptic" axis. This solves the core problems of traditional antidepressants, such as slow onset of action, poor treatment response, and large side effects. It can also improve the core pathological features of depression (neuritis, synaptic plasticity damage) and improve depressive-like behaviors, providing a brand-new option for the clinical treatment of depression.

This invention relates to the field of traditional Chinese medicine extracts and drug preparation technology, and discloses a *Hypericum perforatum* extract composition, its preparation method, and its application, including the application of an ethanol extract of *Hypericum perforatum* or components isolated from its ethanol extract in the preparation of drugs for the prevention and / or treatment of psoriasis. In vitro and in vivo experiments have demonstrated that *Hypericum perforatum* extract can significantly improve imiquimod (IMQ)-induced psoriasis-like skin lesions (erythema, scaling, and skin thickening) in mice and reduce PASI scores. Its mechanism of action differs from single-target biological agents; instead, it simultaneously regulates the IL-23 / IL-17 inflammatory axis, corrects Th17 / Treg immune imbalance, and inhibits abnormal activation of the NF-κB signaling pathway, demonstrating the advantages of multi-component, multi-target synergistic effects of natural drugs. Furthermore, *Hypericum perforatum* is a unique plant resource in my country with a wide distribution. Compared to expensive biological agents, this invention has a significant cost advantage and is expected to be developed into a more affordable and accessible anti-psoriasis drug.

This invention relates to the field of biomedical technology, and more particularly to a polycyclic isopentenylcyclopentanone compound, its preparation method, and its application. The polycyclic isopentenylcyclopentanone compound provided by this invention has the structural formula shown in Formula 1. Using dried fruit of *Hypericum perforatum* as raw material, the extract is obtained through percolation extraction and concentration, and then suspended in water to obtain a suspension. This suspension is then extracted with petroleum ether and concentrated to obtain a crude extract. The crude extract is then separated by normal- and reverse-phase silica gel column chromatography, MCI medium-pressure column chromatography, Sephadex LH-20 gel column chromatography, high-performance liquid chromatography, and chiral column resolution using a Chiral PAK IG to obtain the polycyclic isopentenylcyclopentanone compound. Evaluation of the protective activity of these compounds against cold ischemia-induced AC16 cardiomyocyte injury demonstrates that these compounds can serve as lead compounds for the development of drugs to prevent and treat myocardial cold ischemia injury.