This invention discloses two GST family genes linked to
herbicide resistance and their applications, belonging to the field of
herbicide resistance gene technology. This invention also discloses *
Galium affine* for the first time. GLGST4 , GLGST5 The CDS sequences are shown in SEQ ID NO.1 and SEQ ID NO.4, respectively; this is the first time that expression has been found in
Escherichia coli. GLGST4 , GLGST5 The
gene enables transgenic E. coli to acquire resistance to
diflubenzuron, providing two new
diflubenzuron-resistant GST family genes derived from
Galium cleavers; GLGST4 , GLGST5 The
gene can be used for the breeding of
crop varieties resistant to ALS inhibitor herbicides and the construction and optimization of herbicide-degrading engineered
bacteria. It provides two marker genes for the study of the mechanism of resistance of cleavers to ALS inhibitor herbicides, the detection of cleaver resistance, and the determination of
application time and dosage.