4 results about "Galium" patented technology

The invention provides a quality standard of a rungia pectinata medicinal material and a detection method thereof, and relates to the technical field of traditional Chinese medicines. The invention provides a quality standard detection method for Rungia pectinata medicinal materials, which detects the quality of Rungia pectinata from the aspects of medicinal material sources, character identification, microscopic identification, thin-layer identification, moisture, total ash content, acid-insoluble ash content and extracts, and establishes a thin-layer chromatography identification method with strong specificity, stability and reliability. Therefore, the authenticity of the rungia pectinata medicinal material is identified, the quality of the rungia pectinata medicinal material is evaluated, and the medication safety is ensured. According to the quality standard of the Rungia pectinata medicinal material established by the method, the identification result is stable and reliable, and the accuracy is high.

The invention discloses two GST family genes linked with herbicide resistance and application of the two GST family genes, and belongs to the technical field of herbicide resistance genes. The invention discloses CDS sequences of GLGST4 and GSGST5 of Galium aparine for the first time, and the CDS sequences are respectively shown as SEQ ID NO.1 and SEQ ID NO.4; gLGST4 and GSGST5 genes are expressed in Escherichia coli for the first time, so that transgenic Escherichia coli can obtain drug resistance to florasulam, and two novel florasulam drug-resistant GST family genes derived from Galium aparine are provided; the provided GLGST4 and GSGST5 genes can be used for cultivation of ALS inhibitor herbicide-resistant crop varieties and construction, optimization and transformation of herbicide degradation engineering bacteria, and two marker genes are provided for research of an ALS inhibitor herbicide-resistant mechanism of cleavers, resistance detection of cleavers, and determination of medication time and dosage.

The application discloses a Talaromyces purpureogenus and application thereof in weeding, and belongs to the technical field of bioengineering and weeding, wherein the Talaromyces purpureogenus is named as CY-1, is preserved in the China General Microbiological Culture Collection Center, and has a preservation date of May 23, 2022 and a preservation number of CGMCC No.40203. Metabolic products of the Talaromyces purpureogenus CY-1 in the application can effectively inhibit broadleaf weeds such as amaranthus retroflexus, alternanthera philoxeroides, xanthium, chenopodium album, solanum nigrum, bidens pilosa, comphre, sonchus oleraceus, pteris cretica, medicago sativa, ipomoea hederifolia, rumex patientia, galium aparine, abutilon theophrasti, portulaca oleracea, leonurus japonicus, veronica anagallis, and aster spathulifolius, and the Talaromyces purpureogenus CY-1 is green and efficient in weeding, and has a wide application prospect in weeding.

This invention discloses two GST family genes linked to herbicide resistance and their applications, belonging to the field of herbicide resistance gene technology. This invention also discloses *Galium affine* for the first time. GLGST4 , GLGST5 The CDS sequences are shown in SEQ ID NO.1 and SEQ ID NO.4, respectively; this is the first time that expression has been found in Escherichia coli. GLGST4 , GLGST5 The gene enables transgenic E. coli to acquire resistance to diflubenzuron, providing two new diflubenzuron-resistant GST family genes derived from Galium cleavers; GLGST4 , GLGST5 The gene can be used for the breeding of crop varieties resistant to ALS inhibitor herbicides and the construction and optimization of herbicide-degrading engineered bacteria. It provides two marker genes for the study of the mechanism of resistance of cleavers to ALS inhibitor herbicides, the detection of cleaver resistance, and the determination of application time and dosage.