Application of compound Phyllanthusmin C as herbicide

Abstract

The invention discloses an application of a compound Phyllanthusmin C in serving as a herbicide. A large number of studies show that the compound Phyllanthusmin C separated and purified from the taiwania flousiana has broad-spectrum herbicidal activity; the weeding composition has an obvious inhibition effect on various weeds such as ageratum conyzoides, mikania micrantha, euonymus japonicus, horseweed herb, cleavers, xanthium sibiricum, mazus japonicus, purslane, dayflower, carp intestines, barnyard grass, eleusine indica, digitaria sanguinalis, green bristlegrass, cyperus iria, bidens pilosa, alternanthera philoxeroides, moleplant seed, alopecurus aequalis and cyperus rotundus, is very efficient, and is obviously superior to the existing most common herbicide glyphosate. Besides, the compound Phyllanthusmin C is derived from natural plants, is safe and environment-friendly, has the advantage of environmental friendliness, and has very good application value and prospect in the aspect of developing herbicides.

Description

Technical Field

[0001] The present invention belongs to the technical field of herbicides. More specifically, it relates to the application of the compound Phyllanthusmin C as a herbicide. Background Art

[0002] Weeds are one of the major factors affecting the economy of agriculture and forestry. Common weeds mainly include broad-leaved weeds, grassy weeds, and sedge weeds such as those in the Compositae, Gramineae, Cyperaceae, Labiatae, Leguminosae, Polygonaceae, Cruciferae, Chenopodiaceae, Scrophulariaceae, Caryophyllaceae, Rosaceae, and Umbelliferae families. Broad-leaved weeds, also known as dicotyledonous weeds, mainly include Mikania micrantha, Mikania micrantha, Eupatorium catarium, Galium aparine, Conyza canadensis, Xanthium sibiricum, Mazus japonicus, Portulaca oleracea, Bidens tripartita, etc.; monocotyledonous weeds include Echinochloa crusgalli, Eleusine indica, Digitaria sanguinalis, Setaria viridis, Commelina communis, Potamogeton distinctus, Sagittaria trifolia, Alisma plantago-aquatica, Monochoria korsakowii, Lemna minor, etc.; sedge weeds in the Cyperaceae family are also a type of weeds that seriously harm rice crops, such as Cyperus difformis, Cyperus iria, Kyllinga brevifolia, Fimbristylis miliacea, Cyperus serotinus, Pycreus sanguinolentus, etc.

[0003] For example, Mikania micrantha, listed by the International Union for Conservation of Nature (IUCN) as one of the 100 most harmful invasive species, due to its extremely strong reproductive ability and dispersal ability, rapidly spreads to various agricultural and natural vegetation ecosystems. In tropical and subtropical regions, Mikania micrantha widely occurs in habitats such as forests, orchards, wastelands, farmlands, roadsides, and riverbanks, seriously threatening the ecological safety of various crops such as rubber, fruits, and sugarcane. Due to the high risk, severe harm, and difficulty in prevention and control of Mikania micrantha invasion, it has long been listed in the list of major alien invasive species under key control in China.

[0004] Compared with physical control techniques relying on manual or mechanical means, chemical control techniques relying on herbicides have the advantages of high control efficacy, quick results, and low cost. However, the usage amount of commonly used herbicides such as glyphosate is increasing, resulting in prominent problems such as environmental pollution, phytotoxicity risk, and safety accidents.

[0005] Continuously exploring safe and efficient herbicides has important value. Summary of the Invention

[0006] The present invention aims to explore new safe and efficient herbicides.

[0007] The object of the present invention is to provide the application of the compound Phyllanthusmin C in weed control and the preparation of herbicides.

[0008] Another object of the present invention is to provide the application of plant extracts rich in the compound Phyllanthusmin C in weed control and the preparation of herbicides.

[0009] The above object of the present invention is achieved by the following technical solutions:

[0010] Through a large number of studies, the compound Phyllanthusmin C isolated and purified from Taiwania cryptomerioides has high herbicidal activity against various weeds, showing broad-spectrum herbicidal activity, and is effective against Ageratum conyzoides, Mikania micrantha, Eupatorium catarium, Conyza canadensis, Galium aparine, Xanthium sibiricum, Mazus japonicus, Portulaca oleracea, Commelina communis, Eclipta prostrata, Echinochloa crusgalli, Eleusine indica, Digitaria sanguinalis, Setaria viridis, Cyperus iria, Bidens pilosa, Alternanthera philoxeroides, Leptochloa chinensis, Alopecurus aequalis, Cyperus rotundus, etc.

[0011] Therefore, the present invention claims the following solutions:

[0012] The application of the compound Phyllanthusmin C in weed control.

[0013] The application of the compound Phyllanthusmin C as a herbicide.

[0014] The application of the compound Phyllanthusmin C in the preparation of herbicides.

[0015] The application of a plant extract rich in the compound Phyllanthusmin C in weed control.

[0016] The application of a plant extract rich in the compound Phyllanthusmin C as a herbicide.

[0017] The application of a plant extract rich in the compound Phyllanthusmin C in the preparation of herbicides.

[0018] Specifically, the plant extract rich in the compound Phyllanthusmin C refers to a plant extract whose main active substance is the compound Phyllanthusmin C.

[0019] Optionally, the plant extract is a Taiwania cryptomerioides extract.

[0020] Based on the above applications, the present invention also provides a method for controlling weeds, that is, using the compound Phyllanthusmin C or a herbicide containing the compound Phyllanthusmin C to control weeds.

[0021] As an optional specific implementation, the method for controlling weeds can be applying a herbicide containing the compound Phyllanthusmin C to the target area.

[0022] In addition, in the specific embodiments, the herbicide of the present invention can be applied together with at least one other active ingredient selected from: herbicides, insecticides, fungicides, biological agents, plant growth activators, fertilizers or combinations thereof.

[0023] In the specific embodiments, the herbicide of the present invention may further comprise at least one agrichemically acceptable excipient, adjuvant, carrier, diluent, emulsifier, filler, defoamer, thickener, antifreeze, cryogen, etc.

[0024] As an alternative embodiment, when using compound Phyllanthusmin C, a medicament containing compound Phyllanthusmin C, or a plant extract rich in compound Phyllanthusmin C to control weeds, the reference dosage is that the concentration of compound Phyllanthusmin C is not less than 0.5 mg / L. Preferably, the concentration of compound Phyllanthusmin C is 0.5 - 2 mg / L, or the concentration of compound Phyllanthusmin C is not less than 2 mg / L.

[0025] Preferably, the plant extract rich in compound Phyllanthusmin C is a plant extract with a mass content of compound Phyllanthusmin C not less than 5%.

[0026] The present invention has the following beneficial effects:

[0027] The present invention provides a weeding application of compound Phyllanthusmin C isolated and purified from Taiwania cryptomerioides. This compound has broad-spectrum weeding activity and is very efficient, significantly superior to the most commonly used existing herbicide glyphosate.

[0028] Moreover, the compound Phyllanthusmin C is derived from natural plants, is safe and environmentally friendly, has the advantage of being environmentally friendly, and has very good application value and prospects in the development of herbicides. Specific Embodiments

[0029] The following specific examples are used to further illustrate the present invention, but the examples do not limit the present invention in any form.

[0030] Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field.

[0031] Unless otherwise specified, the reagents and materials used in the following examples are all commercially available.

[0032] The structural formula of compound Phyllanthusmin C is as follows:

[0033]

[0034] The compound Phyllanthusmin C was discovered in our study of the active plant Taiwania flousiana, which is endemic to the eastern Sichuan - western Hubei plant phenomenon center. Taiwania flousiana has various pesticidal activities. Through systematic isolation and purification, the compound Phyllanthusmin C was identified from it. The spectral determination data are consistent with those reported in the literature (Ren Y, Lantvit D D, Deng Y, et al. Potent cytotoxic arylnaphthalene lignan lactones from Phyllanthus poilanei[J]. Journal of Natural Products, 2014, 77(6):1494 - 1504.).

[0035] In the following examples, the compound Phyllanthusmin C used was isolated and purified from Taiwania flousiana by methods such as column chromatography and Sephadex, and its structure was confirmed by nuclear magnetic resonance detection.

[0036] Example 1 Herbicidal Activity Test of Compound Phyllanthusmin C (Ageratum conyzoides, Mikania micrantha, Eupatorium catarium)

[0037] This example tests the herbicidal activity of the compound Phyllanthusmin C against the weeds Ageratum conyzoides, Mikania micrantha, and Eupatorium catarium.

[0038] 1. Herbicidal Activity Determination Method

[0039] (1) Preparation of Medicament

[0040] Accurately weigh an appropriate amount of the compound with an electronic balance, dissolve it with DMSO to prepare a stock solution for use. During the experiment, use a 0.1% Tween - 80 aqueous solution to serially dilute the stock solution to the required concentration as the treatment group for the experiment, and ensure that the volume content of DMSO in the final test system is consistent, which is 1%.

[0041] At the same time, use the same content of DMSO and Tween aqueous solution as the blank control, and use glyphosate (purchased commercially) as the positive medicament control.

[0042] (2) Activity Determination

[0043] The herbicidal activity of the samples was determined by the small cup method. Weed seeds in consistent condition (collected from the teaching and research base of South China Agricultural University) were selected, soaked thoroughly with clean water, and then transferred to a 15% sodium hypochlorite solution and soaked for 10 - 15 min. After soaking, the seeds were rinsed 3 times with distilled water and then germinated for standby. Plastic cups of the same specification were selected, and clean glass beads with a diameter of about 0.4 cm were added. The cups were shaken to make the surface of the glass beads flat, ensuring that the thickness of the glass beads in the plastic cup was about 1.5 cm. Then, the liquid medicine was added until the liquid level was just flush with the glass beads. Next, 15 weed seeds that had germinated and had a consistent and healthy appearance were selected and placed on the glass beads in the plastic cup for cultivation. The cup was sealed with plastic wrap and punctured for ventilation. Each treatment was repeated 3 times.

[0044] The treated plastic cups were placed in a light incubator (photoperiod L∶D = 16 h∶8 h, temperature 25 ± 1 °C, relative humidity 70 ± 5%). A 0.1% aqueous solution of Tween - 80 was supplemented every day to ensure that the liquid level remained unchanged.

[0045] After 7 days of treatment, the root length and fresh weight were measured. The root length inhibition rate and fresh weight inhibition rate were calculated according to the following formula to compare the herbicidal activities of Phyllanthusmin C and glyphosate.

[0046]

[0047] 2. Determination results

[0048] After treatment with different concentrations, the root length inhibition rates and fresh weight inhibition rates of Phyllanthusmin C and the control agent glyphosate on Ageratum conyzoides, Mikania micrantha, and Eupatorium catarium are shown in Tables 1 to 3.

[0049] Table 1 Comparison of the inhibitory effects of Phyllanthusmin C and glyphosate on Ageratum conyzoides

[0050]

[0051] Table 2 Comparison of the inhibitory effects of Phyllanthusmin C and glyphosate on Mikania micrantha

[0052]

[0053] Table 3 Comparison of the inhibitory effects of Phyllanthusmin C and glyphosate on Eupatorium catarium

[0054]

[0055] As can be seen from Tables 1 - 3, at the same concentration, the inhibitory effects of Phyllanthusmin C on Ageratum conyzoides, Mikania micrantha, and Eupatorium catarium are significantly higher than those of glyphosate. Among them, the root length inhibition rate and fresh weight inhibition rate of Phyllanthusmin C on the invasive weed Eupatorium catarium are much higher than those of glyphosate, showing extremely excellent herbicidal effects.

[0056] Example 2 Herbicidal Activity Test of Compound Phyllanthusmin C (Conyza canadensis, Galium aparine, Xanthium sibiricum, Mazus japonicus, Portulaca oleracea, Commelina communis, Eclipta prostrata)

[0057] This example tests the herbicidal activity of compound Phyllanthusmin C against weeds Conyza canadensis, Galium aparine, Xanthium sibiricum, Mazus japonicus, Portulaca oleracea, Commelina communis, and Eclipta prostrata.

[0058] The test method is the same as that in Example 1.

[0059] The test results are shown in Tables 4 to 10.

[0060] Table 4 Comparison of the Inhibitory Effects of Phyllanthusmin C and Glyphosate on Conyza canadensis

[0061]

[0062] Table 5 Comparison of the Inhibitory Effects of Phyllanthusmin C and Glyphosate on Galium aparine

[0063]

[0064] Table 6 Comparison of the Inhibitory Effects of Phyllanthusmin C and Glyphosate on Xanthium sibiricum

[0065]

[0066] Table 7 Comparison of the Inhibitory Effects of Phyllanthusmin C and Glyphosate on Mazus japonicus

[0067]

[0068] Table 8 Comparison of the Inhibitory Effects of Phyllanthusmin C and Glyphosate on Portulaca oleracea

[0069]

[0070] Table 9 Comparison of the Inhibitory Effects of Phyllanthusmin C and Glyphosate on Commelina communis

[0071]

[0072] Table 10 Comparison of the Inhibitory Effects of Phyllanthusmin C and Glyphosate on Eclipta prostrata

[0073]

[0074] The results from Table 4 to Table 10 show that Phyllanthusmin C has good inhibitory effects on the root length and fresh weight of common broad-leaved weeds in farmland such as Conyza canadensis, Galium aparine, Xanthium sibiricum, Mazus japonicus, Portulaca oleracea, Commelina communis, and Eclipta prostrata. At the same concentration, its inhibition rate is equivalent to or even better than that of the control agent glyphosate, showing excellent control effects on broad-leaved weeds.

[0075] Example 3 Herbicidal Activity Test of Compound Phyllanthusmin C (Barnyard Grass, Goosegrass, Crabgrass, Green Foxtail, Nutgrass Galingale)

[0076] This example tests the herbicidal activity of compound Phyllanthusmin C against weeds such as barnyard grass, goosegrass, crabgrass, green foxtail, and nutgrass galingale.

[0077] The test method is the same as that in Example 1.

[0078] The test results are shown in Table 11 to Table 15.

[0079] Table 11 Comparison of the Inhibitory Effects of Phyllanthusmin C and Glyphosate on Barnyard Grass

[0080]

[0081] Table 12 Comparison of the Inhibitory Effects of Phyllanthusmin C and Glyphosate on Goosegrass

[0082]

[0083] Table 13 Comparison of the Inhibitory Effects of Phyllanthusmin C and Glyphosate on Crabgrass

[0084]

[0085] Table 14 Comparison of the Inhibitory Effects of Phyllanthusmin C and Glyphosate on Green Foxtail

[0086]

[0087] Table 15 Comparison of the Inhibitory Effects of Phyllanthusmin C and Glyphosate on Nutgrass Galingale

[0088]

[0089] As can be seen from Table 11 to Table 15, Phyllanthusmin C has good inhibitory effects on the root length and fresh weight of common gramineous weeds and cyperaceae weeds in farmland, such as Echinochloa crusgalli, Eleusine indica, Digitaria sanguinalis, Setaria viridis, Cyperus iria, etc. At the same concentration, its inhibition rate is equivalent to or even better than that of the control agent glyphosate, showing excellent inhibitory activity against gramineous weeds and cyperaceae weeds.

[0090] The above embodiments are the preferred embodiments of the present invention, but the embodiments of the present invention are not limited by the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. Application of compound Phyllanthusmin C in weed control.

2. Use of the compound Phyllanthusmin C as a herbicide.

3. Application of the compound Phyllanthusmin C in the preparation of herbicides.

4. Application of plant extracts rich in Phyllanthusmin C in weed control.

5. Use of plant extracts rich in the compound Phyllanthusmin C as herbicides.

6. Application of plant extracts rich in the compound Phyllanthusmin C in the preparation of herbicides.

7. The use according to any one of claims 4 to 6, characterized in that: The plant extract rich in the compound Phyllanthusmin C refers to a plant extract whose main active substance is the compound Phyllanthusmin C.

8. Application of a Taiwania truncatula extract rich in Phyllanthusmin C in weed control.

9. Use of a Taiwania truncatula extract rich in the compound Phyllanthusmin C as or in the preparation of a herbicide.

10. A method for controlling weeds, characterized in that: The compound Phyllanthusmin C or a herbicide containing the compound Phyllanthusmin C is used to control weeds.

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