2 results about "Digitaria sanguinalis" patented technology

This invention discloses a P450 gene of crabgrass from paddy fields and its application in conferring resistance to cyhalofop-butyl in plants. The expression level of this gene in the resistant crabgrass biotype is significantly higher than that in the sensitive biotype. By constructing it into a plant expression vector and transforming it into maize, the resulting transgenic maize plants were sprayed with 11.25 g ai ha at the 3-4 leaf stage. ‑1 After treatment with cyhalofop-butyl, the maize exhibited significantly higher herbicide resistance and survival rate than wild-type maize. This invention confirms that the crabgrass P450 gene is directly related to herbicide resistance, providing a foundation for using this gene to breed transgenic crops resistant to ACCase inhibitor herbicides, and has broad application prospects in the field of agricultural biotechnology.

This invention discloses a strain of *Boletus urinaria* PP013 and its applications, with the preservation number CGMCC No. 40661. This invention also provides a sustainable, cyclical understory cultivation method, including liquid spawn culture, spawn culture, soil covering for fruiting, and understory inoculation. This method selects shallow-rooted tree species such as *Delonix regia* and *Gardenia jasminoides*, and lays a layer of dry weeds consisting of sugarcane bagasse, miscanthus, and *Digitaria sanguinalis* under the trees. Artificially cultivated fruiting bodies about to open their gills are placed under the trees as a live spore source for spore production. After spore production, the understory is covered with dry weeds and watered. The following year, after fruiting, some fruiting bodies are retained for natural spore production, achieving a single inoculation and multi-year cyclical fruiting. This invention creatively utilizes fruiting body spores for natural inoculation, replacing the traditional direct mycelial inoculation method. It boasts a high success rate of establishment, low long-term production costs, and represents a highly efficient forest-fungus integrated management model.