55 results about "Herbicide glyphosate" patented technology

The invention provides methods for weed control with dicamba and related herbicides. It was found that pre-emergent applications of dicamba at or near planting could be made without significant crop damage or yield loss. The techniques can be combined with the herbicide glyphosate to improve the degree of weed control and permit control of herbicide tolerant weeds.

Stable, concentrated herbicide preparation comprising at least one herbicide with at least one amino group, at least one carboxylic acid group and at least one phosphor containing acid group suspended in finegrained form in a liquid phase, and at least 5% a by weight of a dissolved electrolyte; a process for producing said herbicide preparation and an activating additive (adjuvant) for combination with said herbicide preparation. Preferred suspensions comprise the herbicides glyphosate and glufosinate and the electrolyte ammonium sulphate, acting synergistically. It has not hitherto been possible to incorporate high concentrations of synergistic electrolytes in liquid concentrates of said herbicides.

A high-concentration mixed composition (package-mixcomposition) is proposed, which contains water-soluble pesticides or plant growth regulators, such as the salt of the herbicide glyphosate, and water-insoluble solid pesticides or plant growth regulators, such as the herbicide C Fluflufenafil. The water-soluble pesticide or plant growth regulator is dissolved in the continuous water phase of the composition, while the solid particles of the water-insoluble pesticide or plant growth regulator are suspended in the water phase. These solid particles, individually or in clusters, are closely surrounded by a barrier layer containing one or two water-immiscible organic solvents or emulsifiers with a hydrophilic-lipophilic balance (HLB) of not greater than about 15. The composition may optionally additionally contain a viscosity modifier, such as colloidal hydrophilic silica, dispersed in the aqueous phase. The composition of the present invention exhibits improved inhibition of settling of solid particles and / or increased inhibition of chemical degradation of water-insoluble pesticides or plant growth regulators compared to other similar compositions that do not contain a barrier layer or organic solvent.

The present invention relates to glyphosate N-acetyltransferase (GAT) gene and its application. The present invention screens out one new kind of GAT gene, and introduces the gene into receptor bacterium E. coli BL21 and type plant tobacco to make the gene engineering bacterium strain and the transgenic tobacco obtain raised tolerance on herbicide glyphosate.

The present invention relates to preparation process of carboxylate with modified amorphous copper alloy catalyst and alkaline mixture containing primary alcohol. The modified amorphous copper alloy catalyst prepared through a rapid cooling process is modified with one or several metal elements and non-metallic elements, including Cr, Ti, Nb, Ta, Zr, V, Mo, Si, Mo, W, etc. Under the action of the catalyst and in alkaline condition, primary alcohol is dehydrogenated to prepare carboxylate in fixed yield and high stability. The carboxylate obtained through dehydrogenating diethanolamine may be prepared into herbicide glyphosate through chemical reactions in certain conditions.

The invention relates to a method for identifying the glyphosate resistance of cotton, and the method is used for identifying the glyphosate resistance / tolerance of cotton materials. The method comprises the following steps: seeding cotton seeds in a container or a field; after a certain leaf age of grown cotton, respectively spraying 61.5ai / mu-82g ai / mu or 123g ai / mu-246g ai / mu phytocide glyphosate; and recording the death rate of the cotton, and recording the yield properties while harvesting, wherein the cotton which is sprayed with the 61.5g ai / mu-82g ai / mu glyphosate and dies is sensitive to the glyphosate, the cotton which is sprayed with 123g ai / mu-246g ai / mu glyphosate, does not die and achieves no yield reduction is resistant to the glyphosate, and the cotton which is sprayed with 123g ai / mu-246g ai / mu glyphosate, does not die and achieves yield reduction is tolerant to the glyphosate. The method provided by the invention is suitable for identifying and evaluating the glyphosate resistance / tolerance of cottons of routine variety, hybrid variety and transgenic glyphosate-resistant variety, can be used for accurately measuring the glyphosate resistance / tolerance of the cotton and has strong practicability.

The invention belongs to a pesticide glyphosate synergist and a preparation method thereof, and particularly relates to a herbicide glyphosate isopropamide salt water agent environment-friendly synergist and a preparation method thereof. The synergist disclosed by the invention comprises the following components in percentage by weight: 20-40% of alkyl glycoside, 40-20% of cationic alkyl glycoside, 12-18% of alkyl glycine betaine, 0.5-1.5% of organic silicon, 2.0-5.0% of ammonium sulfate and the balance of water. The preparation method comprises the steps of uniformly stirring the components in equipment with a stirring function at the temperature of 20-80 DEG C. The glyphosate isopropamide salt water agent environment-friendly synergist is high in quality, efficient and environment-friendly.

The invention belongs to the field of genetic engineering, relates to separation, determinate evolution in vitro and functional identification of a resistance gene capable of degrading herbicide glyphosate efficiently, and further relates to the resistance gene capable of degrading herbicide glyphosate in plants. The nucleotide sequence of the gene B3S1 is as shown in the figure SEQ ID NO: 1; the amino acid sequence of encoded protein of the resistance gene is as shown in the figure SEQ ID NO: 2. According to the invention, the glycine oxidase (thiO) from marine bacteria bacillus cereus (Bacillus cereus) is amplified, and two rounds of random mutagenesis and a round of DNA shuffling test are performed continuously on the glycine oxidase (thiO), and combined with an activity screening technology on the basis of T7 phage pyrolysis-horseradish peroxidase / dianisidine enzyme coupling, the final glyphosate oxidase mutant B3S1 is obtained. The function of the gene and the application approach of the gene in degrading the resistance of herbicide glyphosate are proved in the invention.

A process for synthesizing the glyphosate from the alkali metal salt of glycine includes such steps as acidifying with inorganic acid to obtain glycine and inorganic salt, concentrating, drying to obtain the mixture of glycine and salt, adding formaldehyde, alkyl phosphite, tetraamine as catalyst and unitary alcohol as solvent, and reaction to synthesize glyphosate as herbicide while separate out inorganic salt insoluble in the system.

The invention provides a hybridoma cell strain with the preservation serial number of CGMCCNO.10497, and further provides a monoclonal antibody secreted by hybridoma cells and application of the monoclonal antibody for detecting herbicide-resistant glyphosate protein. The monoclonal antibody secreted by the hybridoma cell strain can be used for detecting immunological hybridization of G2-EPSPS protein, and can detect recombined G2-EPSPS protein and G2-EPSPS transgenic plant seeds; no cross reaction happens to the CP4-EPSPS protein, and no cross reaction happens to four types of G2-EPSPS non-transgenic plants of different sources either.

The Bicao mixture is herbicide composition comprising glyphosate herbicide and pyrazosulfuron-ethyl herbicide in the ratio of 10-150, the glyphosate herbicide may be glyphosate, sodium salt of glyphosate, potassium salt of glyphosate, etc., and the pyrazosulfuron-ethyl herbicide may be pyrazosulfuron-ethyl, isopropylamide salt of pyrazosulfuron-ethyl, ammonium salt of pyrazosulfuron-ethyl, sodium salt of pyrazosulfuron-ethyl, potassium salt of pyrazosulfuron-ethyl, etc. The herbicide composition may be compounded into water preparation, water emulsion, wettable powder, soluble powder, oily suspension, dry suspending, water dispersed granular preparation, granular preparation or tablet. It is used in rice field to kill and prevent weed, and has the features of low labor strength, low dosage, low cost, less environmental pollution, etc.

The invention relates to a glycine and an application of an acid salt thereof in preparation of herbicide-glyphosate, and in particular relates to an application of glycine hydrochloride and glycine methyl ester hydrochloride in preparation of the herbicide-glyphosate.

The invention relates to a novel process for recycling waste liquid produced by preparation of weedicide glyphosate (N-phosphono-methyl glycine) by taking alkyl phosphate as a raw material. The process overcomes the disadvantages of mass energy consumption of desalting by hot condensation, large equipment investment, low content of the glyphosate after being heated, low content of the glyphosate in a condensate, large product loss and the like, can well reclaim byproduct salt and can farthest obtain the solid glyphosate. The novel process for recycling mother solution recycles the waste liquid, meets the clean production requirement of energy conservation and emission-reduction and has good economic and social benefits.

The invention discloses a citrus greening disease extinguishing agent. The extinguishing agent is a mixture obtained by using sterile chemical herbicide glyphosate as a raw material and adding certain amount of efficient and low-toxic organic pesticide (such as imidacloprid) into the raw material. The extinguishing agent can be widely used for eradicating citrus trees which infect the citrus greening disease, and kills citrus greening disease medium insect citrus psyllid eating on the sick trees at the same time.

The invention provides a new method for increasing the seed reproduction yield of a rice intelligent sterile line. The new method comprises the following steps: (1) constructing the dominant mutant gene of EPSPS gene, the fertility restorer gene and the pollen inactivation gene of the rice recessive male nuclear sterile gene and a gene for enabling the rice to be sensitive to another type of herbicides in the same T-DNA zone of a vector to form linked genes, and then importing the linked genes into a corresponding sterile line by use of the agrobacterium-mediated transformation technology to form the intelligent sterile line; and (2) carrying out selfing on the intelligent sterile line, and in the process of breeding the intelligent sterile line by use of the group, spraying herbicide glyphosate to remove the sterile plants so as to obtain a consistent fertile plant group. Therefore, the seed reproduction yield of the intelligent sterile line is increased by 1 time in contrast with the seed reproduction yield when the herbicide glyphosate is not applied; besides, the new method has the advantages that sterile and fertile seeds can be screened more accurately and reliably compared with a color sorting method, and is convenient to use.

The invention relates to an expression vector of glyphosate resistance genes GR79 and GAT and application of the expression vector. The invention provides a novel DNA sequence synthesized by optimizing codons of a gene GR79and a gene GATwith high glyphosate tolerance and a plant expression vector pCGG constructed by using double genes, and research results prove that the gene GR79 and the gene GAT in different tissues of the transgenic corn transferred into the plant expression vector can be normally transcribed and translated, the expression quantity of the GR79 protein in the leaves in the seedling stage is 733.15-2203.99 ng / g, the expression quantity of the GAT protein is 101.56-345.84 ng / g, and the two proteins have high expression quantity in corn. Results show that the GR79 and GAT transgenic corn has high resistance to the target herbicide glyphosate, and the expression vector has important significance for synergistically expressing and cultivating the high-glyphosate-resistance plant by using the two genes to transform the plant and applying the high-glyphosate-resistance plant.

A concentrate package-mix composition comprising a water-soluble pesticide or plant growth regulator, for example a salt of the herbicide glyphosate, and a solid water-insoluble pesticide or plant growth regulator, for example the herbicide flumioxazin, is provided. The water-soluble pesticide or plant growth regulator is dissolved in a continuous aqueous phase of the composition and solid particles of the water-insoluble pesticide or plant growth regulator are suspended in the aqueous phase. These solid particles, individually or plurally, are intimately surrounded by a barrier layer, which comprises either one or both of a water-immiscible organic solvent or an emulsifying agent that has a hydrophile-lipophile balance (HLB) not greater than about 15. Optionally the composition further comprises a viscosity modifying agent, such as colloidal hydrophilic silica, dispersed in the aqueous phase. Compositions of the invention exhibit enhanced resistance to settling of the solid particles and / or enhanced resistance to chemical degradation of the water-insoluble pesticide or plant growth regulator by comparison with otherwise similar compositions lacking the barrier layer or the organic solvent.

The invention discloses application of bacillus cereus in degrading pesticide glyphosate. By adopting a bacillus cereus strain to degrade herbicide glyphosate, sewage in a glyphosate production process can be purified, thus the problem of environmental pollution in the glyphosate production process is fundamentally solved.

Soybean plant and seed comprising soybean transgenic event SHZD32-01 and DNA molecules unique to the event. Also provided are use of the plant parts, seeds; the soybean transgenic event SHZD32-01 comprises at least one of the nucleic acid molecules of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 9, and their complete complementary sequences. The method of use include the method for producing soybean with tolerance to herbicide glyphosate, producing a soybean-based commercial product, and controlling weeds in a field comprising soybean plants. Soybean strains comprising the soybean event SHZD32-01 exhibits strong tolerance to glyphosate and is helpful for weeds control. DNA detection of the soybean event SHZD32-01 is useful for identifying the soybean event SHZD32-01 in a sample and may be applied to methods for breeding soybean plants comprising the DNA.

The invention discloses a eleusine indica PFK protein polyclonal antibody as well as a preparation method and application thereof. The invention provides an application of the polyclonal antibody in identification of drug resistance to herbicide glyphosate of eleusine indica. The application has the advantages of short detection period, high sensitivity and strong specificity, and has important application prospects in detection and research of glyphosate target resistance of eleusine indica. The relation between the eleusine indica PFK protein and the herbicide glyphosate resistance is disclosed for the first time, and whether the eleusine indica generates target amplification resistance to the herbicide glyphosate or not can be identified by detecting the content of the PFK protein. Themethod can be used for rapidly identifying the resistance and has guiding significance for scientifically selecting medicines to prevent and eliminate eleusine indica and relieving the generation andspreading of the resistance to glyphosate of eleusine indica.

The invention relates to a planting method for increasing radix polygonati officinalis yield, and belongs to the technical field of agricultural planting. The method comprises the following steps: (1) site selecting, namely, selecting sandy soil which is well-drained and is slightly acidic; (2) planting, namely, ditching after the soil is scarified in October to November, bedding a base fertilizer, covering the upper surface of the base fertilizer with bundled straws, then transversely arranging seeds on the two sides of the straws along the trend of ditches at intervals in a staggered manner, covering the upper surfaces of the straws and the seeds with the soil, and flatly placing pine branches or miscellaneous leaves on the upper surface of the soil; (3) performing field management, namely, spraying 1 percent glyphosate serving as a herbicide, and applying an additional fertilizer twice a year; (4) preventing and controlling plant diseases and insect pests, namely, preventing and controlling grey speck disease, rust disease and insect pests. The method disclosed by the invention has very good sterilizing, water retaining and moisture reserving effects, and has good disease resistance and drought and water-logging resistance; the planted radix polygonati officinalis grows well and is high in yield; the average yield per mu is 6,500 kg when the radix polygonati officinalis is harvested in the third year, and is more than one time of the yield of 3,000 kg per mu achieved by conventional planting; therefore, the planting method is suitable for large-area popularization.

A method of eliciting biological activity of an anionic exogenous chemical substance, for example the herbicide glyphosate, is provided. This method comprises preparing a plant treatment composition having the anionic exogenous chemical substance dissolved or dispersed in water, together with (a) an alkylether surfactant component consisting of one or more surfactants each having the formula: R<12>-O-(CH2CH2O)n((CHR)2O)m-R<13> wherein R<12> is an aliphatic saturated or unsaturated hydrocarbyl group having about 16 to about 22 carbon atoms, n is an average number of about 5 to about 100, m is an average number of 0 to about 5, one R in each-((CHR)2O)-group is hydrogen and the other R is methyl, and R<13> is a hydrogen, C1-4 alkyl or C2-4 acyl group; and (b) an amine surfactant component. The weight ratio of the alkylether surfactant component to the amine surfactant component is about 1:10 to about 10:1; and the alkylether and amine surfactant components are present in total in an adjuvant amount of about 0.05 to about 0.5 parts by weight per part by weight of the anionic exogenous chemical substance, expressed as acid equivalent. The method further comprises applying the plant treatment composition to foliage of the plant within a short period prior to rain or activation of overhead irrigation on the plant.

The invention relates to a technology for decomposing vegetable straws, in particular to arthrobacter sp. and an application of the arthrobacter sp. as a decomposing agent for decomposing the vegetable straws. The arthrobacter sp. is preserved in the China General Microbiological Culture Collection Center on March 17, 2020, and the preservation number of the arthrobacter sp. is CGMCC No. 19486. The invention also discloses an application of the Arthrobacter sp. in the decomposition of the vegetable straw. The arthrobacter sp. disclosed by the invention is obtained by separating corn ground surface soil applied with herbicide glyphosate for a long time, and the degradation rate of glyphosate is 89.7% after the arthrobacter sp. is fermented on a shaking table for 3 days; the strain is mixed with other strains to serve as a high-temperature decomposition agent, the high-temperature decomposition agent is formed by mixing several special strains according to a specific proportion to achieve a synergistic effect, the product contains cellulase, lignin catalase, manganese peroxidase, laccase and the like, and glyphosate can be degraded to inhibit tomato bacterial wilt.

The invention relates to the technical field of biological genetic engineering, in particular to a haematococcus pluvialis chloroplast expression system and application thereof. A sequence as shown inSEQ ID NO:1 and a sequence as shown in SEQ ID NO:2 on a haematococcus pluvialis chloroplast genome are used as homologous arms, a sequence as shown in SEQ ID NO:3 and sequence as shown in SEQ ID NO:4are used as promoters, a sequence as shown in SEQ ID NO:5 and a sequence as shown in SEQ ID NO:6 are used as a terminator, a bar gene is used as a selective marker gene to build a homologous recombination carrier, a particle bombardment is used for guiding the carrier to haematococcus pluvialis cells, and transgenic algal plants are obtained through screening of herbicide glyphosate. By adoptingthe stable haematococcus pluvialis chloroplast expression system, stable expression of exogenous genes in a chloroplast can be realized.

The invention relates to a herbicide, wherein the weight ratio of bensulfuron methyl to isoproturon is (0-15):(45-100). The herbicide can be prepared to wettable powder, suspending agent, water dispersing granule and so on. When the herbicide is applied to control weed in the crop land ridge, the herbicide does not cause crop hurting or killing phenomenon which always happens when the frequently used herbicides glyphosate and paraquat are sprayed, and at the same time, the herbicide reduces the hurt to lungs after a user sprays paraquat herbicides.

The invention relates to the field of plant growth regulation and in particular discloses a method for inducing yield increase of sweet potatoes with glyphosate. The method comprises the following steps: (1) liquid medicine preparation, namely mixing glyhosate ammonium and water, and preparing needed liquid medicine; (2) foliage spray, namely uniformly spraying the liquid medicine prepared in thestep (1) onto sweet potato stems and leaves when the sweet potato stems and leaves grow vigorously, wherein the glyhosate ammonium is glyphosate isopropylamine, the mass concentration of the glyphosate isopropylamine is 41%, and a volume ratio of the glyhosate ammonium to the water is 1:(700-800). According to the method disclosed by the invention, the common herbicide glyphosate firstly serves asan excessive growth control agent so as to perform foliage spray on sweet potatoes. Tests show that the glyphosate isopropylamine has an effect of controlling excessive growth of the sweet potatoes,the yield of the treated sweet potatoes is obviously improved, and the content of soluble sugar and starch is obviously improved. Therefore, the glyphosate is capable of controlling excessive growth of the sweet potato stems and leaves and promoting nutrients of photosynthesis to be conducted to sweet potato roots.

The invention discloses pesticide auxiliary agents for improving the action effect of glyphosate. The pesticide auxiliary agents comprise tributyl phosphate (TBP), dibutyl sebacate (DBS) or diethyl sebacate (DES), wherein the concentration of tributyl phosphate is 200-400 [mu] g / ml, the concentration of dibutyl sebacate is 10-30 [mu] g / ml, and the concentration of diethyl sebacate is 40-60 [mu] g / ml. The pesticide auxiliary agents are simple in dosage form (the three pesticide auxiliary agents are all single dosage forms), can play a significant role in synergism of herbicide glyphosate in agricultural production, reduce the usage amount and production cost of glyphosate, and provide a new thought and lay a working foundation for research and development of efficient and environment-friendly pesticide assistants in the future.