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Arthrobacter sp. and application thereof as decomposition agent for decomposing vegetable straws

The technology of Arthrobacter and decomposing agent is applied in the application field of decomposing agent, which can solve the problems of no biological control, difficult storage and transportation, etc., and achieve the effects of reducing allelopathy, being beneficial to plant absorption, and having strong adsorption capacity.

Pending Publication Date: 2021-04-30
辽宁省微生物科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first liquid decomposing agent recorded in the above literature has the defect that it is not easy to store and transport. The strains constructed in the second decomposing agent are ordinary strains, which will be inactivated by high-temperature composting. Biocontrol function

Method used

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  • Arthrobacter sp. and application thereof as decomposition agent for decomposing vegetable straws
  • Arthrobacter sp. and application thereof as decomposition agent for decomposing vegetable straws
  • Arthrobacter sp. and application thereof as decomposition agent for decomposing vegetable straws

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation and Identification of Arthrobacter SP. CGMCC No.19486

[0031] 1. Isolation of strains

[0032] Weigh 1g of soil sample and add it into 99mL sterile water, shake and incubate on a shaker at 180r / min for 20min, and carry out 10-fold gradient dilution, each dilution is 10 -4 、10 -5 、10 -6 Use a pipette gun to draw 100 μL and evenly spread it on the beef extract solid plate, place it in a constant temperature incubator at 45°C, and pick a single colony after 24 hours.

[0033] The strains isolated from the soil were inoculated respectively on the sodium carboxymethylcellulose medium, cultured in a 45°C incubator for 24 hours, stained with 1% Congo red for 1 hour, decolorized with 5% NaCl for 1 hour, and the diameter of the hydrolysis circle was observed. The cellulase-producing strain X-1 was screened out.

[0034] The inoculated strain X-1 was cultured in 100mL inorganic salt culture solution containing 100mg / L glyphosate at 30°C and 180r / min in a ...

Embodiment 2

[0045] 1) The effect of Arthrobacter on glyphosate degradation: Inoculate the activated X-1 seeds in the fermentation medium, the volume of the fermentation bottle is 100mL / 500mL, the initial pH of the fermentation medium is 6.0, and the fermentation medium is heated at 45°C and 180r / min Cultivate under the conditions for 72 hours, measure the residual amount of glyphosate by HPLC, calculate the degradation rate, and the degradation rate reaches 89.7%.

[0046] The fermentation medium: add glyphosate at a final concentration of 100 mg / L to the sterilized inorganic salt basic culture solution. Wherein, the inorganic salt medium (g / L) is: MgSO 4 ·7H 2 O 0.2g, CaCl 2 2H 2 O 0.03g, FeSO 4 ·7H 2 O 0.01g, KH 2 PO 4 0.4g,Na 2 HPO 4 0.6 g, MnSO 4 0.02g,NH 4 NO 3 1.0g, distilled water 1L. Adjust the pH to 7.0. Described HPLC measures and adopts Kromasil-C18 column (150mm * 4.6mm, 5 μ m); Mobile phase V (methanol): V (water)=70: 30, wherein the phosphoric acid that con...

Embodiment 3

[0050] The preparation of embodiment 3 decomposing agent

[0051] 1) Strain activation to obtain seed liquid:

[0052] ①According to the conventional existing technology, pick a ring of Phanerochaete chrysosporium that has been activated and rejuvenated and inoculate it into (per 100mL water addition) glucose 1.8, yeast extract 1.5, potassium dihydrogen phosphate 0.03, magnesium sulfate 0.03, In a liquid medium composed of pH 6.0, culture at 42° C. with ventilation for 72 hours to obtain a seed solution for use.

[0053] ②Inoculate the activated and rejuvenated Bacillus subtilis or Arthrobacter respectively in the liquid culture medium according to the conventional existing technology, and culture in a shake flask at 45°C for 24 hours; among them, the liquid culture medium (addition amount per 100mL water): peptone 1, yeast extract 0.5, sodium chloride 0.5, PH7.0,.

[0054] 2) Solid culture

[0055] ① After microscopic examination of the obtained seed liquid without any bac...

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Abstract

The invention relates to a technology for decomposing vegetable straws, in particular to arthrobacter sp. and an application of the arthrobacter sp. as a decomposing agent for decomposing the vegetable straws. The arthrobacter sp. is preserved in the China General Microbiological Culture Collection Center on March 17, 2020, and the preservation number of the arthrobacter sp. is CGMCC No. 19486. The invention also discloses an application of the Arthrobacter sp. in the decomposition of the vegetable straw. The arthrobacter sp. disclosed by the invention is obtained by separating corn ground surface soil applied with herbicide glyphosate for a long time, and the degradation rate of glyphosate is 89.7% after the arthrobacter sp. is fermented on a shaking table for 3 days; the strain is mixed with other strains to serve as a high-temperature decomposition agent, the high-temperature decomposition agent is formed by mixing several special strains according to a specific proportion to achieve a synergistic effect, the product contains cellulase, lignin catalase, manganese peroxidase, laccase and the like, and glyphosate can be degraded to inhibit tomato bacterial wilt.

Description

technical field [0001] The invention relates to a technology for decomposing vegetable straws, in particular to an Arthrobacter and its application as a decomposing agent for decomposing vegetable straws. Background technique [0002] With the rapid development of my country's horticultural industry, the output of vegetables has increased year after year. The output of vegetable straw is second only to rice, corn and wheat straw, and has become the fourth largest crop straw. However, most straws are randomly piled up and discarded, spreading germs, polluting the environment, and causing a lot of waste of nutrients. About half of the photosynthetic products in vegetable straws have a cellulose content of about 30%, a protein content of about 5%, and a certain amount of minerals, such as calcium and phosphorus. Microbial fermentation can decompose these organic matters into components that plants can directly absorb and utilize, and realize the resource utilization of vegetab...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F11/08C05F17/20C12R1/06C12R1/125C12R1/645
CPCC12N1/20C12N1/14C05F11/08C05F17/20Y02W30/40
Inventor 刘晓辉池景良李杨陈顺高晓梅敖静孙玉禄桓明辉郭玲玲王红旗李鑫宗玉丽
Owner 辽宁省微生物科学研究院
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