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Method for preparing marine actinomyces and metabolite thereof and application

A technology for marine actinomycetes and metabolites, applied in the directions of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., to achieve the effects of easy operation, good emulsifying type and stability, and obvious effects

Inactive Publication Date: 2011-05-25
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no effective pesticide available. The most economical and effective way to prevent and control the disease is to cultivate and plant peanut varieties resistant to bacterial wilt and adopt new methods such as biological control. Therefore, it is important to find new active biological metabolites for the prevention and treatment of bacterial wilt. one of the ways

Method used

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  • Method for preparing marine actinomyces and metabolite thereof and application
  • Method for preparing marine actinomyces and metabolite thereof and application
  • Method for preparing marine actinomyces and metabolite thereof and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] From more than 2000 kinds of actinomycetes isolated from offshore sea mud, the strain Streptomyces yangjiang was screened by the zone of inhibition method to have obvious inhibitory effect on R. solanacearum. The strain was classified and identified as follows:

[0042] (1) Morphological observation

[0043] The strain Streptomyces yangjiang was cultured as inserts, and the hyphae in the substrate, aerial hyphae and whether sporocyx were formed; the shape of the sporocyx; whether there were spores formed; Samples were taken at 7d, 14d, 21d and 28d, respectively, and observed with an optical microscope and a scanning electron microscope.

[0044] It grows well on Gao's No. 1 medium, air silk II 61' gray, base silk blue, spore silk gray in color, producing light yellow pigment. Observed under an optical microscope, the basal filaments are not broken, the aerial hyphae form spore filaments, and the spore filaments are straight and flexible. Observed under a scanning elec...

Embodiment 2

[0083] Preparation of metabolites of Streptomyces yangjiang that have significant inhibitory effects on bacterial wilt, such as image 3 and 4 Shown:

[0084] (1) Cultivation of Streptomyces yangjiang

[0085] Cultivate Streptomyces yangjiang at 28°C using slant culture until the mycelia and spores mature;

[0086] The culture medium is the improved Gaoshi No. 1 medium, and its composition is as follows: 20 g of soluble starch, KNO 3 1g, seawater essence 3g, K 2 HPO 4 ·3H 2 O 0.66g, FeSO 4 ·7H 2 O 0.02g, MgSO 4 ·7H 2 O 1.03g, agar 16g, pH value 7.2~7.4, water 1000ml, sterilize at 115~121℃ for 30min;

[0087] (2) Fermentation of Streptomyces yangjiang

[0088] A, preparation of Streptomyces Yangjiang seed liquid: insert the mycelia and spores on the slope into the fermentation medium, and cultivate them at 160 rpm for 48 hours at 30°C to obtain the Streptomyces Yangjiang seed liquid;

[0089] B. Fermentation of Streptomyces yangjiang: Inoculate the seed solution of ...

Embodiment 3

[0100] Preparation of metabolites of Streptomyces yangjiang that have a significant inhibitory effect on bacterial wilt:

[0101] (1) Cultivation of Streptomyces yangjiang

[0102] The slant culture prepared by using the modified Gaoshi No. 1 medium is based on cultivating Streptomyces yangjiang at 30°C until the mycelia and spores mature;

[0103] (2) Fermentation of Streptomyces yangjiang

[0104] A, preparation of Streptomyces Yangjiang seed liquid: insert the mycelia and spores on the inclined surface into the fermentation medium, and cultivate them at 180rpm for 60h at 28°C to obtain the Streptomyces Yangjiang seed liquid;

[0105] B, the fermentation of Streptomyces Yangjiang: Streptomyces Yangjiang seed liquid is connected in the fermentation medium (same as Example 2) with the inoculum size of 10% by volume, at 28 ℃ with the rotating speed of 400rpm, the air of 10~15L / min Aeration was carried out for 90 hours of fermentation;

[0106] (3) Preparation of metabolites ...

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Abstract

The invention discloses a method for preparing marine actinomyces and metabolite thereof and application. A strain of marine actinomyces is obtained by separating from near sea mud through an inhibition zone method, and the metabolite of the marine actinomyces has remarkable inhibiting effect on pseudomonas solanacearum. The marine actinomyces is named as Yangjiang streptomyces and is preserved in China Center for Type Culture Collection on July, 31st, 2009, and the preservation number is CCTCC NO: M209167. The invention also discloses a preparation method for the metabolite with inhibiting effect on pseudomonas solanacearum in the marine actinomyces. The preparation method prepares the metabolite by controlling the fermentation conditions of the Yangjiang streptomyces, extracting mycelium and separating the fermented supernate and the mycelium extract through silica-gel column chromatography. The preparation method is simple and feasible and has low cost. A preparation prepared by using the metabolite for preventing and controlling the pseudomonas solanacearum has good effect and good stability, and belongs to low-toxicity and low-residue pesticide.

Description

technical field [0001] The invention belongs to the field of biopesticide research, in particular to a preparation method and application of marine actinomycetes and their metabolites. Background technique [0002] Peanut is an important economic crop and oil crop in my country, and bacterial wilt (Pseudomonassolanacearum) is a devastating disease on peanut. The incidence area of ​​peanut bacterial wilt in my country is about 300khm 2 , South China is the key incidence area of ​​bacterial wilt, the incidence rate is usually 10 to 20%, especially the most serious at the seedling stage, and the serious loss of the disease reaches more than 70%. At present, there is no effective pesticide available. The most economical and effective way to prevent and control the disease is to cultivate and plant peanut varieties resistant to bacterial wilt and adopt new methods such as biological control. Therefore, it is important to find new active biological metabolites for the prevention ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04A01N63/02A01P1/00C12R1/465
Inventor 林壁润郑奕雄蒲小明沈会芳周光雄高向阳唐晓翠
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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