148 results about "Lotus japonicus" patented technology

The invention discloses application of a hevea brasiliensis HbCPT8 gene in promoting plant growth and increasing gum yield, and belongs to the field of genetic engineering.The hevea brasiliensis HbCPT8 gene is transferred into kok-saghyz through a plant expression vector to obtain a transgenic kok-saghyz positive plant, and it is found through comparison of the phenotype of the transgenic kok-saghyz and the gum content of the root of the transgenic kok-saghyz that the hevea brasiliensis HbCPT8 gene can be used for promoting plant growth and increasing gum yield. Compared with wild type kok-saghyz, the biomass and the rubber yield of the transgenic kok-saghyz are remarkably improved, which shows that the HbCPT8 gene plays an important role in regulating and controlling rubber synthesis and has important significance in cultivating rubber plant varieties capable of increasing the rubber yield.

The invention discloses a panax notoginseng MYB transcription factor gene PnMYB8, the nucleotide sequence of the gene PnMYB8 is as shown in SEQ ID NO: 1, an MYB transcription factor is encoded, molecular biology and functional genomics related technologies prove that the PnMYB8 gene has the capability of improving fungal infection resistance of plants, the PnMYB8 gene is constructed on a plant expression vector and transferred into tobacco for overexpression, and the yield of the panax notoginseng MYB transcription factor gene PnMYB8 is improved. Experimental results show that the resistance of the transgenic tobacco overexpressed with the PnMYB8 to the tryptophan destructor (Cylindrocarpon destructor), the fusarium latericum (Fusarium latericum), the fusarium equiseti (Fusarium equiseti) and the phoma herbarum (Phoma herbarum) is obviously enhanced, and the resistance of the transgenic tobacco overexpressed with the PnMYB8 to the tryptophan destructor (Fusarium latericum) is obviously enhanced.

The invention provides a CsERECTA gene-based tea tree leaf shape regulation and control method and a tea tree light utilization rate improving method, which are characterized in that a sequence of a tea tree gene is cloned to obtain a complete sequence; then, the CsERECTA is subjected to bioinformatics analysis, expression of a GUS reporter gene is started by constructing a plant expression vector and connecting a target fragment, and a recombinant plant vector is used for genetic transformation of tobacco, so that the function of the CsERECTA is verified; the character of the CsERECTA transgenic line is analyzed, the leaf shape is determined, the method capable of improving the leaf shape of the tea tree is obtained, and a foundation is laid for cultivating a new variety and improving the light utilization rate of the tea tree by using the CsERECTA gene.

The invention discloses application of a scutellaria baicalensis SbMYB8 gene in promotion of anthocyanin synthesis, the application comprises the steps that the SbMYB8 gene is constructed to a plant expression vector and introduced into plant cells to promote synthesis of plant anthocyanin, and the nucleotide sequence of the SbMYB8 gene is shown as SEQ ID NO: 1, or the amino acid sequence coded by the SbMYB8 gene is shown as SEQ ID NO: 2. The MYB transcription factor SbMYB8 gene with the function of promoting anthocyanin synthesis is cloned and identified from the scutellaria baicalensis for the first time, and the blank of a transcription regulation mechanism for regulating anthocyanin metabolism in the scutellaria baicalensis is filled; the SbMYB8 gene can significantly promote accumulation of anthocyanin in various plants, shows good heterologous expression stability and adaptability, and has wide cross-species application potential.

The invention relates to a method for cultivating insect-resistant transgenic Xiaoblack poplar, in particular to a method for cultivating insect-resistant transgenic Xiaoblack poplar. The invention aims to solve the problem of low insect resistance of the populus similis. The cultivation method comprises the following steps: 1, synthesizing a Cry1Ac gene, wherein the nucleotide sequence of the gene is shown as Seq ID No: 1 in a sequence table; 2, a plant expression vector pBI121-Cry1Ac expressed by the Cry1Ac gene is constructed, and the plant expression vector pBI121-Cry1Ac is constructed; 3, preparing an infection solution; 4, cultivating the Cry1Ac-transgenic small black poplar by using an agrobacterium-mediated leaf disc transformation method, the Cry1Ac-transgenic poplar is created, the average concentration of leaf Cry1Ac protein is more than 200ng / g, the insect resistance of the transgenic poplar can be improved, all fall webworms die after being fed for 5 days, and the corrected death rate reaches 100%. The method is applied to the field of molecular breeding.

The invention belongs to the field of plant biotechnology and genetic engineering, and particularly relates to a method for transient expression of genes in tea-oil tree leaves. The method comprises the following steps: 1) preparing an agrobacterium infection solution of a plant expression vector containing a target gene, wherein the optimum OD600 value of the agrobacterium infection solution is 0.6-0.9; 2) using a disposable sterile syringe needle to perform scribing and injury making on the back of a non-in-vitro mature camellia oleifera leaf with the leaf age of 6-12 months, sucking the infection liquid in the step 1) into a syringe and abutting against the wound for injection until the infection liquid diffuses from the wound to the whole leaf; (3) immediately wrapping the leaves with tin foil paper, and carrying out shading treatment for 24-36 hours; and (4) detecting the expression condition of the target gene of the transgenic leaves on the second to sixth days after treatment. According to the method disclosed by the invention, instantaneous overexpression of the target gene of the camellia oleifera is realized by injecting mature leaves of the camellia oleifera, and functional verification of the target gene in a camellia oleifera body can be quickly and efficiently realized.

The invention belongs to the field of biological gene engineering, and relates to application of a ginseng transcription factor PgERF2 in improvement of plant resistance to alternaria alternata, and the sequence of the ginseng transcription factor PgERF2 protein is as shown in SEQ ID NO.1; the sequence of the ginseng transcription factor PgERF2 gene is as shown in SEQ ID NO. 2. According to the invention, the PgERF2 gene is cloned, a corresponding plant expression vector is constructed, and the gene is successfully transferred into wild type arabidopsis thaliana. A transcriptomics analysis result shows that the response to biological stress in a transgenic plant is activated, the expression level of the plant defense protein related gene AtPDF1.2 A is remarkably improved, and it is proved that PgERF2 can be directly combined with a GCC-box element to activate the expression of the plant defense protein related gene AtPDF1.2 A, so that the resistance to alternaria alternata is remarkably enhanced.

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS1 A dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA3301 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS1 It is constructed from genes. SsNHX1 Genes and SsSOS1 The nucleotide sequences of the gene are shown in SEQ ID NO:1 and SEQ ID NO:2. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantages are: the synergistic effect of the two genes effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

The invention discloses a method for establishing a pea genetic transformation system. The method comprises the following steps: step 1, transforming agrobacterium competent cells by using a plant expression vector; step 2, preparing and storing agrobacterium liquid; step 3, preparation of aseptic pea seedlings; step 4, infection and co-culture of the explants; step 5, restoring culture; step 6, bud induction culture; step 7, carrying out bud elongation culture; and step 8, rooting culture. According to the method, the pea variety Zhongwan No.6 which is easily subjected to a genetic transformation test is screened, the test verifies that the optimal explant is the cotyledonary node, and the bud induction rate of the cotyledonary node is 80% or above. According to the method, the problem of rooting difficulty is solved, a complete regenerated plant is obtained, the pea plant with whitening phenotype is successfully obtained by transforming the CRISPR / Cas9 gene editing carrier of the targeted pea PDS gene, the genetic transformation efficiency is improved to 5% or above, and the genetic transformation efficiency of peas is greatly improved.

The invention discloses an oil-controlling and skin-astringing composition as well as a preparation method and application thereof, and particularly belongs to the field of preparations for dressing and makeup. The composition for controlling oil and astringing skin is prepared from the following components in parts by weight: 1.5 to 2.1 parts of tea leaf extract, 7.1 to 8.6 parts of hamamelis virginiana water, 0.003 to 0.01 part of moso bamboo leaf extract, 0.036 to 0.051 part of lotus corniculatus seed extract and 0.6 to 1.2 parts of alkanet extract. The oil-controlling and skin-astringing composition provided by the invention has excellent performance of inhibiting grease secretion of human sebaceous gland cells, and has an excellent deep oil-controlling effect on skin; the resident skin external preparation provided by the invention has excellent effects of short-time oil control, lasting oil control and skin astringing.

The invention discloses application of a tea tree CsGT-3b gene in improving the disease resistance of plants and a method of the tea tree CsGT-3b gene. The CsGT-3b gene is a Trihelx transcription factor family gene, the CsGT-3b gene is constructed to a plant expression vector and is transferred into tobacco, a transgenic tobacco plant is obtained through stable genetic transformation, and the CsGT-3b gene shows relatively strong fungal inhibitory activity on botrytis cinerea inoculation; according to the present invention, the CsGT-3b gene on the tea tree leaf is subjected to instantaneous silencing by using the antisense oligonucleotides (AsODNs) technology, such that the disease resistance of the plant on the lasiodiplodia theobroma is significantly weakened, and the CsGT-3b gene on the tea tree leaf is subjected to the enzyme-linked immunosorbent assay so as to significantly reduce the disease resistance of the plant on the lasiodiplodia theobroma; therefore, the CsGT-3b gene has the effect of improving the disease resistance of the plants, can be used as a disease-resistant gene, can improve the disease resistance of the plants by being introduced into the plants such as tobacco, tea trees or vegetables, and has a wide market application prospect.

The present invention concerns the use of at least one plant extract capable of activating the GDF11 (Growth Differentiation Factor 11) growth factor and a cosmetic or pharmaceutical composition comprising at least one extract as an active ingredient for treating hair growth. The at least one extract is chosen from the following: • an extract rich in peptides and sugars derived from Scabiosa arvensis cell cultures; • an extract rich in peptides and sugars derived from Lotus japonicus cell cultures enriched in somatic embryos; • a hydroethanolic extract from artichoke flower heads belonging to the species Cynara scolymus; and / or a water-soluble extract derived from prickly pear pads in combination with a hydroethanolic extract derived from coffee beans.

The present invention is applicable to the field of genetic engineering technology and provides a cloning and application of the Sophora alopecuroides (Solanum sophora) SaPEI7 gene. By screening differentially expressed genes from transcriptome sequencing of Sophora alopecuroides under simulated stress conditions, a Sophora alopecuroides gene related to salt tolerance was identified through bioinformatics analysis as the Sophora alopecuroides (Solanum sophora) pectin methylesterase inhibitor protein gene SaPEI7. Quantitative detection using RT-PCR technology revealed a significant increase in gene expression under salt stress conditions. The gene was functionally verified by constructing a plant expression vector and successfully transformed into Arabidopsis thaliana. The results showed that overexpression of the gene in Arabidopsis thaliana improved its salt tolerance, providing a new resource for improving crop stress resistance through genetic engineering technology.

The invention belongs to the technical field of plant genetic engineering and biology, and particularly relates to application of a muskmelon CmEXPB1 gene to improvement of fruit crack resistance. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. According to the invention, the gene CmEXPB1 is cloned from muskmelon cDNA, and a real-time fluorescence quantification technology is utilized to find that the expression quantity of the gene in anti-cracking sweet melon peel is obviously higher than that of easily-cracking peel. A plant expression vector is constructed and transformed into tomatoes, and it is found that peel epidermis hardness, cortical layer toughness and compactness of a transgenic line are remarkably higher than those of a wild type, and brittleness is remarkably reduced; cell wall component determination finds that the contents of cellulose, hemicellulose and protopectin of the transgenic tomato are increased, and the contents of soluble pectin and lignin are reduced, so that the CmEXPB1 is proved to enhance the crack resistance by adjusting cell wall components. The invention lays a theoretical foundation for researching the fruit crack resistance, and also provides a gene resource for culturing horticultural plants with high-crack-resistance fruits.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a spartina anglica ascorbic acid peroxidase SanAPX11 gene and application, the nucleotide sequence of the SanAPX11 gene is shown as SEQ ID NO.1, and the amino acid sequence of protein coded by the SanAPX11 gene is shown as SEQ ID NO.2. After the SanAPX11 gene is transferred into a cell in the form of a plant expression vector, the SanAPX11 gene is expressed in a plant cell, so that the stress resistance of the plant, particularly drought stress resistance, oxidative stress resistance, salt stress resistance and the like, can be improved.

The invention discloses application and a method of an iaaM gene in creating a new chrysanthemum flower type, and belongs to the field of plant genetic engineering and ornamental plant molecular breeding. The complete CDS sequence of the iaaM gene derived from agrobacterium tumefaciens is constructed into a plant expression vector and is introduced into a chrysanthemum plant, so that stable overexpression of iaaM in a chrysanthemum body is realized. By improving the level of endogenous auxin of plants, the corolla tube fusion of the ligulate flowers is obviously promoted, the flat petal flower is changed into the key petal flower, and the directional improvement of the flower is realized. Compared with traditional cross breeding, the method has the advantages of being short in breeding period, low in genetic background complexity, controllable in target character and the like, and an effective molecular breeding strategy is provided for precise regulation and control of chrysanthemum flower types.

This invention discloses a gene, AcMYB22, that regulates the biosynthesis of flavonoids in kiwifruit and its applications, belonging to the field of genetic engineering technology. This invention clones two alleles of the AcMYB22 transcription factor, AcMYB22-1 and AcMYB22-2, from the 'Hongyang' kiwifruit and its bud mutations. Plant expression vectors of AcMYB22-1 and AcMYB22-2 were constructed, and overexpressed kiwifruit fruits and stably overexpressed kiwifruit plants were obtained using Agrobacterium-mediated transformation. The overexpressed materials showed a promotion of flavonoid and anthocyanin accumulation. This research enriches the mechanism by which MYB transcription factors regulate the biosynthesis of flavonoids in kiwifruit, provides technical support for improving plant flavonoid synthesis, and is of great significance for the quality improvement and new variety breeding of kiwifruit.

Disclosed are use of the Camellia sinensis (L.) O. Kuntz CsMYB1 gene in improving disease resistance of a plant and a method for improving disease resistance of a plant. The CsMYB1 gene is a transcription factor gene of the MYB family. CsMYB1 is constructed into a plant expression vector, and the vector is transferred into Nicotiana benthamiana. A transgenic tobacco plant is obtained by means of stable genetic transformation, and the plant exhibits relatively strong fungal inhibitory activity against inoculated Botrytis cinerea hyphae. Transient silencing of the CsMYB1 gene of Camellia sinensis (L.) O. Kuntz leaves using a technology of antisense oligonucleotides (AsODNs) significantly reduces the disease resistance of the plant. Therefore, the CsMYB1 gene has the effect of improving the disease resistance of a plant and can be used as a disease-resistant gene. Introducing the gene into plants such as tobacco, Camellia sinensis (L.) O. Kuntz, or vegetables can improve the disease resistance of these plants. The present invention has broad market application prospects.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an expression promoter of a PubHLH47 gene to improvement of salt stress resistance of populus japonica, and the nucleotide sequence of the PubHLH47 gene is as shown in SEQ ID NO.1. The PubHLH47 gene provided by the invention is utilized to construct a plant expression vector, so that the PubHLH47 gene is over-expressed in the populus japonica, and the salt stress resistance of the populus japonica is improved. A PubHLH47 gene overexpressed plant has the characteristic of effectively improving the tolerance of the plant under the stress of high salt. The PubHLH47 gene capable of improving the salt stress resistance is found in forest for the first time, an excellent gene resource is provided for promoting forest genetic engineering breeding, and a scientific basis is provided for later improvement of populus nigra.

The invention discloses a nucleotide sequence of a larch endogenous strong promoter Pro-LarGRP1A and an application of the larch endogenous strong promoter Pro-LarGRP1A. The invention relates to a method for creating and cultivating woody gymnosperm plants such as larch with precisely improved target agronomic characters such as biomass, disease resistance, insect resistance, stress resistance and the like through genetic transformation of a carrier expressed by a target gene driven by a Pro-LarGRP1A promoter sequence and a method for creating and cultivating the woody gymnosperm plants such as the larch with precisely improved target agronomic characters such as biomass, disease resistance, insect resistance, stress resistance and the like. The implementation effect shows that compared with the known conventional plant expression vector, the expression vector constructed by taking the nucleotide sequence of the Pro-LarGRP1A promoter region disclosed by the invention as the promoter element is used for transforming a larch receptor material through an agrobacterium-mediated genetic transformation method, a target gene in a positive transformed strain can be efficiently expressed, and the expression vector has the advantages that the expression vector is simple and convenient to construct, and the application prospect is wide. And the gene expression level is more than 8 times that of the conventional expression vector. The nucleotide sequence of the larch endogenous strong promoter Pro-LarGRP1A and the application of the larch endogenous strong promoter Pro-LarGRP1A disclosed by the invention have an important application value in efficient and low-cost cultivation of new germplasm, new materials and new varieties of woody gymnosperm plants such as high-biomass, high-volume, high-stress-resistance and high-quality larch.

The present invention belongs to the field of biological genetic engineering and relates to the use of ginseng transcription factor PgERF2 in improving plant resistance to Alternaria alternata. The sequence of the ginseng transcription factor PgERF2 protein is shown in SEQ ID NO.1; the sequence of the ginseng transcription factor PgERF2 gene is shown in SEQ ID NO.2. The present invention clones the PgERF2 gene and constructs a corresponding plant expression vector, successfully transferring the gene into wild-type Arabidopsis thaliana. Transcriptomic analysis results show that the response to biotic stress is activated in the transgenic plants, and the expression level of the plant defense protein-related gene AtPDF1.2A is significantly increased. It is also demonstrated that PgERF2 can directly bind to the GCC-box element to activate the expression of the plant defense protein-related gene AtPDF1.2A, thereby significantly enhancing resistance to Alternaria alternata.

This invention relates to a multi-gene tandem plant expression vector for improving soybean alkali tolerance, its construction method, and its application, belonging to the field of genetic engineering technology. To address the technical problem that simple superposition of multiple genes in soybean sodium salt tolerance improvement easily leads to pathway interference, increased metabolic burden, and poor growth, making it difficult to systematically improve alkali tolerance, this invention utilizes homologous recombination to express genes from the same metabolic pathway... GmNAC133 , GmC2H2 , GmPET6 By constructing a multi-gene tandem plant expression vector and utilizing a soybean hairy root transformation method based on the RUBY reporter gene, a systematic improvement in soybean alkali tolerance was successfully achieved. This invention is not only applicable to improving soybean alkali tolerance but also provides an efficient and reliable technical approach for breeding other crops that are difficult to genetically transform or require multi-gene synergistic regulation, and has broad prospects for widespread application.

This invention relates to the field of plant molecular biology, specifically to a loquat fatty acid desaturase-related gene, EjFAD8, its encoded protein, and its applications. The full-length cDNA sequence of this gene is shown in SEQ ID No. 1, and the amino acid sequence of its encoded protein is shown in SEQ ID No. 2. The EjFAD8 gene of this invention increases plant cold resistance by increasing the synthesis of SQDG (16:0 / 18:1) and promoting the expression of related genes in the ICE-CBF-COR low-temperature stress pathway. Using Agrobacterium tumefaciens Gv3101-mediated flower-dip method, the plant expression vector pCambia2300-35S::EjFAD8-eGFP containing the target gene was transformed into wild-type Arabidopsis thaliana. Overexpression of 35S::EjFAD8 in the transgenic Arabidopsis enhanced its cold resistance. This invention utilizes transgenic Arabidopsis thaliana plant materials obtained from the loquat EjFAD8 gene. Under low temperature stress, these plants can increase the content of unsaturated fatty acids, enhance the expression of low temperature-related genes, and reduce the content of H2O2 in the plant, thereby increasing the plant's cold resistance and showing great application potential.

The application discloses application of a calcium-dependent protein kinase gene GhCDPK29 in plant resistance to verticillium wilt, and belongs to the technical field of genetic engineering. The gene GhCDPK29 is amplified by using sequence information of the gene, a VIGS (Virus Induced Gene Silencing) plant expression vector is constructed, the VIGS plant expression vector is transformed into Gossypium hirsutum TM-1, and transgenic cotton is obtained; after inoculation of cotton verticillium V991, the transgenic cotton shows resistance to verticillium wilt, which indicates that the gene GhCDPK29 is highly related to the resistance of cotton to verticillium wilt. The application lays an application foundation for research on a molecular mechanism of verticillium wilt resistance, cultivation of new plant varieties with verticillium wilt resistance, and provides a new reference for screening of new disease-resistant genes and cultivation of disease-resistant plants.

The invention discloses application of a sweet potato IbbHLH149 gene in improvement of plant stress resistance, and belongs to the technical field of molecular biology. The nucleotide sequence of the sweet potato IbbHLH149 gene is as shown in SEQ ID NO.1, and the coded amino acid sequence of the sweet potato IbbHLH149 gene is as shown in SEQ ID NO.2. The method comprises the following steps: constructing a plant expression vector of the IbbHLH149 gene, and transforming arabidopsis thaliana; the result shows that the morphological development of the arabidopsis thaliana plant into which the IbbHLH149 gene is transferred is normal, and the arabidopsis thaliana seedling into which the IbbHLH149 gene is transferred can at least resist the stress of 125mM NaCl high salt or the stress of 400mM mannitol; the expression of the IbbHLH149 gene in arabidopsis thaliana can significantly improve the salt tolerance and drought resistance of the arabidopsis thaliana. Therefore, the IbbHLH149 gene has an important application value in the cultivation of stress-resistant plants.

The invention belongs to the field of plant genetic engineering, and particularly relates to application of a WIND4 gene to improvement of genetic transformation efficiency of taxus chinensis. The WIND4 gene is constructed to a plant expression vector containing a Ruby report system, taxus chinensis cells are transformed through an agrobacterium-mediated method, on the premise that cell growth and development are not affected, rapid screening of positive cells can be achieved, division of transformed cells is promoted at the same time, and therefore transformation efficiency is improved. According to the invention, the synergistic interaction effect of WIND4 and Ruby is utilized, so that the defect of weak regeneration capacity of taxus chinensis cells is overcome. Compared with an empty vector control group, the WIND4 and Ruby co-expression can improve the transformation efficiency of taxus chinensis by about 1.4 times; compared with the traditional method, the conversion system disclosed by the invention is shorter in conversion period and simpler and more convenient to operate.

Glucuronosyltransferase 1 gene which catalyzes glucuronic acid transfer to the hydroxyl group at the 3-position in an oleanane-type triterpenoid is identified. Glucuronosyltransferase 1 gene having a desired activity, derived from a Fabaceae plant (soybean, Glycyrrhiza, and Lotus japonicus), and containing nucleotide sequences represented by SEQ ID Nos: 2, 4, and 6, respectively, is provided.

This invention relates to a hybrid tulip tree LhSAMDC gene and its applications, belonging to the field of genetic engineering technology. The CDS nucleotide sequence of the LhSAMDC gene described in this invention is shown in SEQ ID NO.1, and the encoded adenosine methionine decarboxylase plays a key role in plant polyamine metabolism. This invention also provides primer pairs (SEQ ID NO.2 and SEQ ID NO.3) for amplifying this gene and a plant expression vector containing this gene. Experiments using transgenic Arabidopsis thaliana show that overexpression of the LhSAMDC gene significantly increases plant biomass, plant height, leaf SPAD value, and stress resistance. This invention provides a new technical means for plant breeding, which can be used to cultivate new plant varieties with stronger growth vigor, higher biomass, and better stress resistance, and has broad application prospects.

The present invention discloses the sweet potato IbNFYA3 gene and its application in improving plant stress resistance, belonging to the technical field of molecular biology. The amino acid sequence encoded by the sweet potato IbNFYA3 gene of the present invention is shown in SEQ ID NO.2, and its nucleic acid sequence is shown in SEQ ID NO.1. A plant expression vector of the IbNFYA3 gene was constructed and transformed into Arabidopsis thaliana. The results showed that Arabidopsis plants transformed with the IbNFYA3 gene had normal morphological development, and that the transgenic Arabidopsis seedlings could withstand at least 125mM NaCl stress or 300mM mannitol stress. Expression of the IbNFYA3 gene in Arabidopsis thaliana significantly improved its high salt tolerance and drought resistance. Therefore, the IbNFYA3 gene has important application value in the cultivation of new drought-resistant and salt-tolerant germplasms.

The application discloses a method for creating a fragrant glutinous millet by using a CRISPR / Cas12i.3 system and application thereof, and belongs to the technical field of genetic engineering. The application first applies a miniaturized CRISPR / Cas12i.3 gene editing system with independent intellectual property rights of China to the genetic improvement of millet, and establishes an efficient and precise gene editing technology system suitable for the crop. Compared with commonly used Cas9 and other systems, the Cas12i.3 protein has a smaller volume, is easier to construct a plant expression vector, significantly improves the transformation efficiency, and avoids foreign patent restrictions, thereby providing a key tool for functional gene research and molecular design breeding of millet.