8 results about "Lotus japonicus" patented technology

This invention relates to the field of plant genetic engineering technology, and more particularly to... SsNHX1 and SsSOS1 A dual-gene plant expression vector and its application in improving salt tolerance in alfalfa. The plant expression vector uses pCAMBIA3301 as its backbone and inserts genes derived from Suaeda salsa. SsNHX1 Genes and SsSOS1 It is constructed from genes. SsNHX1 Genes and SsSOS1 The nucleotide sequences of the gene are shown in SEQ ID NO:1 and SEQ ID NO:2. Using Agrobacterium tumefaciens EHA105-mediated transformation, the vector was introduced into the leaves of sterile alfalfa seedlings. Positive transgenic plants were obtained through co-culture, callus induction differentiation, and PCR identification. The advantages are: the synergistic effect of the two genes effectively enhances the salt tolerance of alfalfa, enabling it to grow normally under 200–300 mM NaCl stress, providing a new solution for forage cultivation in saline soils.

This invention relates to a multi-gene tandem plant expression vector for improving soybean alkali tolerance, its construction method, and its application, belonging to the field of genetic engineering technology. To address the technical problem that simple superposition of multiple genes in soybean sodium salt tolerance improvement easily leads to pathway interference, increased metabolic burden, and poor growth, making it difficult to systematically improve alkali tolerance, this invention utilizes homologous recombination to express genes from the same metabolic pathway... GmNAC133 , GmC2H2 , GmPET6 By constructing a multi-gene tandem plant expression vector and utilizing a soybean hairy root transformation method based on the RUBY reporter gene, a systematic improvement in soybean alkali tolerance was successfully achieved. This invention is not only applicable to improving soybean alkali tolerance but also provides an efficient and reliable technical approach for breeding other crops that are difficult to genetically transform or require multi-gene synergistic regulation, and has broad prospects for widespread application.

This invention relates to the field of plant molecular biology, specifically to a loquat fatty acid desaturase-related gene, EjFAD8, its encoded protein, and its applications. The full-length cDNA sequence of this gene is shown in SEQ ID No. 1, and the amino acid sequence of its encoded protein is shown in SEQ ID No. 2. The EjFAD8 gene of this invention increases plant cold resistance by increasing the synthesis of SQDG (16:0 / 18:1) and promoting the expression of related genes in the ICE-CBF-COR low-temperature stress pathway. Using Agrobacterium tumefaciens Gv3101-mediated flower-dip method, the plant expression vector pCambia2300-35S::EjFAD8-eGFP containing the target gene was transformed into wild-type Arabidopsis thaliana. Overexpression of 35S::EjFAD8 in the transgenic Arabidopsis enhanced its cold resistance. This invention utilizes transgenic Arabidopsis thaliana plant materials obtained from the loquat EjFAD8 gene. Under low temperature stress, these plants can increase the content of unsaturated fatty acids, enhance the expression of low temperature-related genes, and reduce the content of H2O2 in the plant, thereby increasing the plant's cold resistance and showing great application potential.

This invention discloses a P450 gene of crabgrass from paddy fields and its application in conferring resistance to cyhalofop-butyl in plants. The expression level of this gene in the resistant crabgrass biotype is significantly higher than that in the sensitive biotype. By constructing it into a plant expression vector and transforming it into maize, the resulting transgenic maize plants were sprayed with 11.25 g ai ha at the 3-4 leaf stage. ‑1 After treatment with cyhalofop-butyl, the maize exhibited significantly higher herbicide resistance and survival rate than wild-type maize. This invention confirms that the crabgrass P450 gene is directly related to herbicide resistance, providing a foundation for using this gene to breed transgenic crops resistant to ACCase inhibitor herbicides, and has broad application prospects in the field of agricultural biotechnology.

This invention discloses a transcription factor gene of Minjiang lily. LrbHLH1 Its nucleotide sequence is as described in SEQ ID NO: 1, encoding a protein with the amino acid sequence shown in SEQ ID NO: 2. This invention has confirmed this through reverse genetics technology. LrbHLH1 The gene has the function of enhancing plant resistance to fungi, and the present invention will... LrbHLH1 Genes were constructed into plant expression vectors and overexpressed in tobacco. The transgenic tobacco plants exhibited strong resistance to fungal infection, demonstrating the effectiveness of overexpression. LrbHLH1 The genetically modified tobacco plants exhibit high levels of resistance to infection by *Alternaria alternata* and *Alternaria solani*.

The application provides application of OpCBR1 transcription factor in improving growth of short small snake grass hairy roots and increasing camptothecin yield, and the application comprises the following steps: constructing a plant expression vector containing an OpCBR1 gene, and genetically transforming short small snake grass stem section explants to obtain short small snake grass hairy roots over-expressing the OpCBR1 gene; using a weighing method and a high performance liquid chromatography method to analyze the biomass and the camptothecin yield of the transgenic hairy roots, and the results show that over-expression of the OpCBR1 gene can improve the biomass of the short small snake grass hairy roots, thereby improving the camptothecin yield; through dynamic observation, it is found that the biomass of the hairy roots of the transgenic strain of OpCBR1 is significantly improved on the 16th day, and reaches the maximum on the 32nd day, and the yield also reaches the maximum, which indicates that over-expression of the OpCBR1 gene can obviously shorten the culture period. The application provides a new high-quality drug source with broad-spectrum anticancer effect for producing camptothecin, and has important theoretical significance and potential application value.

This invention belongs to the field of fruit tree resistance gene application technology, and relates to an apple transcription factor MYB79 gene and its application in improving apple resistance to the woolly aphid. The amino acid sequence of the protein encoded by the MYB79 gene is shown in SEQ ID NO:1, and the nucleotide sequence is shown in SEQ ID NO:2. This invention constructs a plant expression vector carrying the MYB79 gene and recombinant Agrobacterium, and obtains MYB79-overexpressing apple plants through Agrobacterium-mediated transformation. Functional verification results show that MYB79 gene overexpression can significantly improve apple resistance to the woolly aphid, reduce the feeding efficiency and survival rate of the woolly aphid, and significantly shorten the feeding time of the woolly aphid in the phloem of the transgenic plants, resulting in a significant decrease in survival rate, while having no significant impact on the normal growth and development of the apple plants. This invention provides new gene resources and technical support for molecular breeding of apples resistant to the woolly aphid and innovation of insect-resistant germplasm.

This invention discloses the application of the Masson pine PmVNS2 gene, belonging to the field of plant genetic engineering technology. The invention obtains the Masson pine PmVNS2 gene through cloning, its nucleotide sequence is shown in SEQ ID NO.1, and its expressed protein sequence is shown in SEQ ID NO.2. The invention constructs a plant expression vector 35S::PmVNS2 and transforms it into poplar leaves, cultivating and screening to obtain poplar PmVNS2 transgenic plants. Compared with control plants, the number of roots, plant height, and ground diameter are significantly increased. Real-time quantitative PCR revealed that PmVNS2 significantly promotes the upregulation of expression levels of most key enzyme genes and transcription factor encoding genes in the secondary xylem synthesis of poplar; further scanning electron microscopy results show that the number of secondary xylem cell layers and the cell wall thickness in heterologously expressed transgenic poplar are significantly increased. This invention provides an effective molecular tool and method for improving poplar traits and has broad application prospects.