45 results about "Gene targeting" patented technology

The present application relates to the field of plant genetic engineering technology, in particular to the application of ZmAAAP59 protein and its coding gene in regulating corn tolerance to high temperature stress, by knocking out ZmAAAP59 gene in corn, it is determined that the mutant has obvious high temperature tolerance phenotype, it is verified that corn ZmAAAP59 gene can negatively regulate plant heat resistance, by reducing the expression amount of ZmAAAP59 gene in plants, the heat resistance of plants can be effectively increased.The discovery of the heat resistance function of ZmAAAP59 gene provides a new gene target and resource for breeding heat-resistant plant varieties, has important significance for the study of the heat tolerance molecular mechanism of corn, and lays a certain theoretical foundation for the study of the mechanism of plant response to high temperature stress and the molecular mechanism of resisting adverse environment.

The application belongs to the technical field of biology, and discloses application of CmPHL1 transcription factor in inhibition of citrus leaf oil cell formation and regulation of volatile substances. In view of the problem of insufficient research on existing citrus oil cell formation and essential oil synthesis, a pGBI-CmPHL1 overexpression vector is constructed, and positive strains are obtained by means of Agrobacterium-mediated transformation of citrus. Experiments prove that overexpression of CmPHL1 can significantly reduce the number of oil cells and the content of monoterpenes, sesquiterpenes and other volatile substances. CmPHL1 targets the molecular regulation network of oil cell development, accurately regulates and is heritable, provides a new gene target for citrus quality improvement and breeding, is in line with the direction of green agriculture, and has important economic and ecological benefits.

This invention discloses a method for preparing a co-loaded dual-gene targeted delivery system and its application, involving the construction and preparation of a dual-gene (pHNF4α, pFOXA3) delivery system. This delivery system can target myofibroblasts (MFs) and activated hepatic stellate cells (aHSCs) and can be used for gene therapy of liver fibrosis. This invention synthesizes the targeting carrier material PEI. 25K -PEG 2K -pPB, through electrostatic adsorption, combines with pHNF4α and pFOXA3 to form a co-loaded dual-gene targeted delivery system, F4α / A3@PP-pPB. This delivery system, through the targeting group pPB, binds to MFs (metacellular matrix cells) that lead to excessive accumulation of extracellular matrix in fibrotic livers and to PDGFR (polydimethylformamide) highly expressed on the surface of aHSCs. On the one hand, it inhibits the activation of aHSCs; on the other hand, it transdifferentiates MFs into hepatocyte-like cells (iHep). These two modes, while combating liver fibrosis, can also achieve a certain degree of liver function reconstruction through transdifferentiated iHep cells, providing new ideas and methods for the effective treatment of liver fibrosis.

The present application relates to the technical field of plant breeding, in particular to StERF1A Application of a gene related biological agent in improving the resistance of potato to bacterial wilt, the biological agent StERF1A The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The present application discloses for the first time StERF1A As a negative regulatory factor involved in the mechanism of potato resistance to bacterial wilt: overexpression StERF1A Can increase the accumulation amount of bacterial wilt in leaves, inhibit the expression of disease resistance related genes Pto 、 PR1b1 And PR1a The content of active oxygen ROS and the activity of related enzymes SOD, POD and CAT are reduced; and the expression of down-regulation StERF1A The opposite effect is presented, which significantly enhances the resistance of potato to bacterial wilt. The present application provides an important gene target and theoretical basis for breeding new potato varieties with high resistance to bacterial wilt.

This invention belongs to the field of biological breeding technology, specifically involving SlCSLA2 Application of genes in improving the storage and transportability of tomato fruit. This invention addresses the industry problem of severe storage and transport losses in tomatoes due to excessive softening after harvest. For the first time, it utilizes CRISPR / Cas9 gene editing technology to knock out the cell wall hemicellulose (mannan) synthase gene. SlCSLA2 This invention has led to the creation of a tomato mutant with significantly enhanced fruit firmness. The firmness of the entire fruit during the red-ripe stage is 35%–42% higher than that of the wild type, and the firmness can be maintained for about a week after harvest, effectively delaying the softening process and significantly improving storage and transportability. This invention provides new gene targets and technical approaches for breeding tomato varieties with improved storage and transportability.

The application belongs to the technical field of biological medicine, and provides a CXCR4 high expression type iPSC-NK cell with enhanced bone marrow and tumor tissue homing ability, and a preparation method and application thereof. The cell takes pluripotent stem cells as starting cells, overexpresses a membrane-bound IL-15 and IL-15RA fusion protein gene and a CXCR4 receptor gene in the pluripotent stem cells; pluripotent stem cells stably expressing the target gene are obtained, and then iPSC-NK cells are obtained through induction differentiation. The membrane-bound IL-15 and IL-15RA fusion protein gene and the CXCR4 receptor gene are targetedly integrated into a safe harbor site of the induced pluripotent stem cell through a gene editing technology, so as to construct an iPSC cell strain stably expressing key proteins; the function-enhanced iNK cell is obtained through induction differentiation, and exhibits excellent bone marrow and various solid tumor tissue homing ability and persistent immune killing activity.

The application provides a bovine cell gene editing reagent and a construction method and application of a BCO2 gene mutant cell strain, and belongs to the technical field of gene editing. The application provides a bovine cell gene editing reagent, which comprises BE4max SpCas9 V922 Δ mRNA and mRNA containing a target gene target point sequence and gRNA. When the BE4max-SpCas9 V922 Δ mRNA is combined with the gBCO2-1 mRNA to transfect target cells in bovine mammary epithelial cells (Mac-T), the cytosine base editing function in the cells can be normally exerted, and the editing of the bovine BCO2 gene target point is successfully realized.

The application discloses CTL-S1 A gene and an application of miR-let-7 in regulation of reproductive development of drosophila bacterea CTL-S1 The gene is specifically highly expressed in ovaries of drosophila bacterea, and the expression of the gene is inhibited by RNA interference CTL-S1 The expression of the gene can reduce the hatching rate of offspring. Through analysis of an RNA immunoprecipitation double luciferase reporter system, it is found that miR-let-7 and CTL-S1 there is a target relationship. Overexpression of miR-let-7 can cause CTL-S1 the expression amount of the gene to be significantly reduced, and a phenotype similar to the expression of the gene being inhibited by RNA interference CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S1 CTL-S is generated, and the hatching rate of offspring is reduced. The application provides a potential gene target for developing a genetic control strategy for destroying the reproduction of drosophila bacterea, provides a new idea for subsequent biological control of insects by using the sterile technique, and has a wide application prospect.

This invention relates to the field of biotechnology and discloses a method for regulating the angle of fruiting branches in upland cotton. GhPDF1 Genes and their applications, the aforementioned GhPDF1 The coding region sequence of the gene is shown in SEQ ID NO:1; GhPDF1 The gene ID is GH_D04G0386.1 ;A sort of GhPDF1 The application of genes in regulating the fruiting branch angle of upland cotton, either for regulating the fruiting branch angle of upland cotton or for breeding upland cotton varieties with a compact plant type, is described. GhPDF1 Genes regulate the fruiting branch angle of upland cotton by modulating their expression levels in upland cotton cells. This invention provides a precise genetic modification pathway, demonstrating the ability to inhibit [the growth of] upland cotton fruiting branches using VIGS technology. GhPDF1 Gene expression can significantly increase the angle between cotton fruiting branches, making the plant type looser, providing a clear gene target and effective technical means for using genetic engineering technology to cultivate new cotton varieties with compact plant type.

This invention provides a microfluidic chip for the multiplex differentiation and diagnosis of different avian influenza virus subtypes and its application. Specifically, it is a microfluidic chip based on TaqMan probes that can simultaneously and rapidly differentiate and diagnose 25 avian influenza subtypes, enabling high-throughput simultaneous detection of multiple gene targets. This invention utilizes high-throughput microfluidic chip technology to simplify the differential diagnosis procedure for avian influenza virus subtypes, resulting in more accurate and time-saving results. It is more suitable for rapid clinical diagnosis of avian influenza, providing technical support for rapid clinical detection, diagnosis, and prevention of avian influenza, and is of great significance for epidemic prevention and control and safeguarding public health security.

Methods and compositions are provided for enhancing the accumulation and / or recovery of recombinant casein expressed in soy. Endogenous Casein-Processing Interfering Proteins (CPIPs), such as oxidases like lipoxygenases (LOX), are present in the recombinant protein fractions and interfere with yield and / or purity. RNA interference (RNAi) mechanisms are leveraged in plasmids that knockdown genes expressing CPIPs and / or genes in the Soybean Vesicular Trafficking Pathway (SVTP). Knocking down of two or more target genes is manifested using a dual gene targeting approach, often via a single expression cassette containing multiple distinct shRNA constructs for enhanced efficiency. The shRNA constructs invoke a RNAi mechanism that leads to increased casein accumulation either: (1) directly, by suppressing CPIPs (e.g., LOX) to enable more efficient purification; or (2) indirectly, by modifying SVTP genes to route casein away from lytic vacuoles, preventing degradation.

This invention relates to the field of biotechnology, specifically disclosing a Decacovirus nucleic acid detection composition based on RT-RPA and CRISPR / Cas13a technologies and its applications. This invention successfully obtains a novel method for Decacovirus nucleic acid detection based on RT-RPA and CRISPR / Cas13a technologies. The nucleic acid detection composition includes: an RT-RPA primer pair and crRNA; the RT-RPA primer pair consists of two RT-RPA primers, with sequences shown in SEQ ID NO.1 and SEQ ID NO.2; the crRNA sequence consists of an anchoring sequence for binding to the Cas13a protein and a guide sequence targeting the Decacovirus gene target sequence, with the guide sequence shown in positions 23-50 of SEQ ID NO.3. Using this composition for Decacovirus detection offers advantages such as high specificity, high sensitivity, rapid and convenient operation, solving the problem of inconvenience in current field monitoring of the virus's prevalence.

The application discloses a kind of similar bundle-like arabino-galactoside protein coding gene GhFLA2 In the application of regulating cotton seed vigor, the application obtains several overexpression materials, RNA interference materials and gene knockout materials by transgenic technology GhFLA2 It is found that overexpression GhFLA2 Can promote radicle elongation during seed germination and cotyledon unfolding after germination, GhFLA2 After the expression amount of the application is reduced or knocked out, radicle elongation and cotyledon development are inhibited.The application provides a new gene target for cotton seed vigor improvement.

The application of OsFabF gene in resistance to rice stripe virus, the application relates to the technical field of rice disease resistance breeding. The application uses a CRISPR / Cas9 gene editing system to design sgRNA targeting a specific functional region of an endogenous OsFabF gene in rice, to precisely edit the region, thereby obtaining a mutant with a functionally deficient OsFabF. The editing preferably results in a frame shift mutation or a premature stop codon, to obtain a truncated OsFabF protein mutant. Experiments show that the rice mutant plant obtained in this way exhibits significantly enhanced resistance to RSV infection, reduced virus accumulation and reduced disease symptoms. The application provides an effective gene target and breeding material for cultivating new rice varieties resistant to rice stripe virus.

Provided are an INHBE gene-targeted double-stranded oligonucleotide, a conjugate, and a composition, which relate to the technical field of nucleic acid drugs. The double-stranded oligonucleotide comprises a sense strand and an antisense strand. The antisense strand is complementary or substantially complementary to the sense strand; the sense strand comprises a nucleotide sequence identical or substantially identical to at least 15 contiguous nucleotides in a sequence of SEQ ID NO: 309, and said substantially identical means that there is no more than three nucleotide differences between the sense strand and the at least 15 contiguous nucleotides in the sequence of SEQ ID NO: 309. The double-stranded oligonucleotide can effectively inhibit the INHBE gene and treat patients suffering from metabolic diseases or metabolic syndrome and related diseases such as diabetes, hypertension and cardiovascular diseases.

The application discloses a specific primer, a probe combination and a method for rapidly detecting toxoplasma gondii based on RAA fluorescence method, and relates to the technical field of molecular biology detection. The specific primer sequence is shown as SEQ ID No: 2 and SEQ ID No: 10; the probe sequence is shown as SEQ ID No: 12, and is modified with a FAM-dT, THF and BHQ1-dT ternary fluorescent quenching structure and a 3' end C3-Spacer at specific positions. The primer and the probe combination can be used to complete amplification and fluorescence detection of the toxoplasma gondii WX2 gene target in 20 min under a constant temperature condition of 39 DEG C, and the sensitivity reaches 10 copies / muL. The method is suitable for rapid screening of toxoplasma gondii for non-diagnostic purposes.

This invention discloses a PRX gene and its application, as well as a peroxidase protein, relating to type III peroxidases and their applications. The peroxidase protein encoded by the PRX gene, screened through a dry season database transcriptome, can participate in the synthesis of lignin in citrus peel. The PRX genes CgPRX24 / CgPRX41 / CgPRX65 of this invention can participate in the synthesis of lignin in citrus peel, promoting the firmness of the citrus peel. Simultaneously, they can serve as gene targets for genetic engineering and biopreservation of citrus, aiming to improve the peel texture during postharvest storage.

The application discloses a SiLARGE3 gene related to millet yield and an encoded protein and application thereof. The genomic DNA sequence of the SiLARGE3 gene is shown as SEQ ID NO. 1, the promoter sequence is shown as SEQ ID NO. 2, the coding sequence is shown as SEQ ID NO. 3, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 4. The application discloses that the SiLARGE3 gene is a negative regulation factor of the millet yield. By knocking out the SiLARGE3 gene through a gene editing technology, loss-of-function mutants (silarge3-1 and silarge3-2) are obtained. Compared with a wild type, the kernel size (kernel length, kernel width and 1000-grain weight) is significantly increased, the number of secondary branch and the number of grains per spike are significantly increased, and finally the millet yield is increased by 25.0%-30.7%. The application further provides a method for increasing the millet yield by using the SiLARGE3 gene and the mutants thereof. The application provides a new gene target and operation method for high-yield breeding of the millet, and has important theoretical and application values.

This application presents a gRNA probe library, its preparation and design methods, a method for targeting genes, and a nucleic acid imaging method. This application combines gRNA probes containing modified groups with nucleases, enabling dynamic observation of DNA and RNA by binding to target genomic sequences or RNA transcripts in living cells. By utilizing fluorescently labeled crRNAs, tracrRNAs, and sgRNAs, this application achieves real-time imaging of repetitive and non-repetitive sites, establishing a powerful gene imaging toolkit. Furthermore, the gRNA probe library prepared in this application will be applied to CRISPR systems for gene editing, transcriptional regulation, gene targeting, and delivery.

The application relates to the technical field of gene targeting regulation, and provides application of siRNA targeting RNF24 in preparation of a liver cancer treatment drug. The sense strand and the antisense strand of the siRNA targeting RNF24 have nucleotide sequences shown in SEQ ID NO. 1-SEQ ID NO. 10, the siRNA can treat liver cancer by inhibiting the expression of an RNF24 gene and reducing the synthesis of a protein coded by the RNF24 gene, and can reduce the proliferation and migration abilities of liver cancer cells. The application solves the problems that existing liver cancer molecular targeted treatment drugs are limited, sequence silencing efficiency and specificity are difficult to be considered in siRNA targeted treatment, stability is insufficient, and delivery efficiency is low.

The application belongs to the field of biotechnology and particularly relates to ABC110 gene and application thereof in improving natural rubber yield of guayule. A nucleic acid molecule is provided, and the nucleotide sequence of the nucleic acid molecule is shown in SEQ ID NO:1 or SEQ ID NO:2. The application also provides application of the nucleic acid molecule in cultivating guayule plants with improved root biomass and natural rubber content. Overexpression of the nucleic acid molecule in guayule can significantly increase the taproot diameter of guayule, increase the dry root weight of each guayule plant and improve the natural rubber content of the roots of guayule. The application provides a new gene target and method for genetic improvement of guayule and has a wide application prospect in cultivation of high-yield guayule varieties.

The invention provides herein epigenomic changes identified in old mice compared to young mice or old mice treated with Oct4, Sox2, Klf4 and c-Myc (OSKM). The invention further provides methods of identifying a compound by analyzing one or more PRC2-associated epigenomic change in a sample before and after contacting the sample with one or more compounds. Also provided herein are methods of using compounds identified using the methods described herein for improving proliferative capacity, cell and tissue regeneration, and / or reducing fibrosis.

Disclosed herein are modifications of polynucleic acid molecules such as inhibitory polynucleic acid molecules and pharmaceutical compositions comprising modified polynucleic acid molecules. Further, provided herein are methods for utilization of modified polynucleic acid molecules as well as methods for suppressing an expression of gene targeted by the modified polynucleic acid molecules.

The present application relates to a DNA drug, a gene targeting delivery system and its application in the treatment of lower extremity peripheral arterial disease. Specifically, the present application relates to a microcrystalline nanocarbon targeted drug delivery system and a plasmid containing human interleukin-4 gene loaded thereon. The delivery system comprises a matrix microcrystalline nanocarbon, and folate, polyethyleneimine and polyethylene glycol modified on the surface thereof. The matrix microcrystalline nanocarbon has a size of 5-20 nanometers, a crystallinity of >80%, a rich mesoporous structure, a high specific surface area and good biocompatibility. The present application also provides a preparation method of the system, which comprises the steps of synthesizing a matrix by pulse modulation radio frequency plasma enhanced chemical vapor deposition, surface functionalization modification and plasmid loading. After injection into the lower extremity muscle, the system can actively target the macrophages in the ischemic site, realize long-term expression of the IL-4 gene at the lesion site. It can effectively promote stable, mature functional angiogenesis by inducing macrophages to polarize to the repair M2 phenotype and regulating the OSM / GSNOR / ENG signal axis, thereby improving limb ischemia. The present application has important application value in the field of gene therapy for ischemic diseases, especially peripheral arterial disease and severe limb ischemia.

The present invention relates to the technical field of biomedicine, and in particular to a recombinant oncolytic virus expressing a bispecific or trispecific single-chain antibody, and the use thereof. The recombinant oncolytic virus comprises an antibody gene integrated into a viral backbone thereof. The antibody gene comprises a target antibody gene targeting tumor cells and at least one antibody gene targeting immune cells. The immune cells are selected from any one or two of T cells, NK cells and macrophages. When the antibody gene comprises one target antibody gene targeting tumor cells and one antibody gene targeting immune cells, the antibody gene is a bispecific antibody. When the antibody gene comprises one target antibody gene targeting tumor cells and two antibody genes targeting immune cells, the antibody gene is a trispecific antibody. By means of integrating a gene encoding a bispecific single-chain antibody or a trispecific single-chain antibody into a modified oncolytic virus, the gene for the bispecific single-chain antibody or the trispecific single-chain antibody can be expressed in vivo, thereby further improving the therapeutic effect on tumor cells.

This invention discloses a PSR primer, kit, and detection method for identifying Pediococcus lactis based on a specific gene target. Based on the conserved sequence of the target gene, this invention designs and optimizes PSR-specific primers, establishes a PSR amplification system, and further provides a kit containing the primers and its application method. The PSR detection method established by this invention features high sensitivity, strong specificity, rapid reaction, simple operation, and low cost. It is suitable for the rapid and accurate identification of Pediococcus lactis and has promising application prospects in food fermentation, probiotic detection, and microbial quality control.

A system for producing a therapeutic oligomer includes a computing device configured to design a proposed therapeutic oligomer sequence, wherein designing further comprises generating a genomic library for an organism from a gene target, initiating a sequence identification function, identifying a genomic locus that the proposed therapeutic oligomer sequence is predicted to bond to as a function of an off-target sequence function, selecting the proposed therapeutic oligomer sequence as a function of the sequence identification function, the genomic locus, and a criterion element, and synthesize a therapeutic oligomer as a function of the proposed therapeutic oligomer sequence.

This invention provides a glyceraldehyde-3-phosphate dehydrogenase mutant and its application, the mutant being based on Streptococcus mutans Source gapN The original sequence was used to obtain information about rational mutation design. gapN The effective site mutation of the amino acid coding sequence was obtained, and through gene editing, the coding genes of the wild-type sequence, the mutant coding gene, and the mutant combination were integrated into the genome of *Corynebacterium glutamicum* to obtain a new strain. The lysine production of the new strain was significantly increased, further demonstrating that... gapN Mutations at three sites in the gene have a significant effect on lysine production, providing important gene targets and engineering strategies for constructing industrial strains that produce L-lysine efficiently.

The application discloses application of an OsCATA gene in controlling rice heading stage and belongs to the technical field of genetic engineering. The application finds for the first time that the OsCATA gene (cDNA sequence as shown in SEQ ID NO:1) derived from rice Nipponbare is involved in the regulation of the rice heading stage, and knocking out the gene can significantly delay the heading of rice under short-day and long-day conditions; under long-day, the OsCATA gene regulates the heading stage in a manner dependent on OsELF3-1, down-regulates Ghd7 to inhibit the expression of Ehd1, Hd3a and RFT1, and finally delays the heading. The application also provides a knocking-out vector and method of the OsCATA gene and obtained mutant plants, the oscata single mutant delays the heading by 12.4 days under the long-day in Hangzhou and by 7.2 days under the short-day in Hainan, and the oself3-1 oscata double mutant has a more significant delay effect. The application provides a new gene target for the regulation of the rice heading stage, perfects the photoperiodic flowering regulation network of rice, and the provided method and mutant can be used for the directional breeding of new rice varieties suitable for different photoperiod regions, and has important breeding application value.

Provided in the present application are a gRNA probe library and a preparation method and design method therefor, and a method for targeting a target gene and a nucleic acid imaging method. In the present application, a gRNA probe containing a modifying group is bound to a nuclease, which binds to a target genomic sequence or RNA transcript in a living cell, thereby realizing dynamic observation of DNA and RNA. By using fluorescently labeled crRNAs, tracrRNAs and sgRNAs, the present application realizes real-time imaging of repetitive and non-repetitive loci and establishes a robust gene imaging toolkit. In addition, the gRNA probe library prepared in the present application will be applied to a CRISPR system for gene editing, transcriptional regulation, and gene targeting and delivery.