The present invention relates to a method for excising a
nucleic acid sequence from the
genome of a
plant or a
plant cell. This method is based on the steps of transforming a
plant cell with a construct encoding
a DNA double strand break inducingenzyme (DSBI), generating a transgenic plant line, performing a transient
assay to analyze the functionality of the transgenic
enzyme, crossing the plant line with a line containing a
nucleic acid sequence to be excised and performing an immature
embryo conversion or a
tissue culture regeneration through
callus formation. The method can also be reversed, which means that a
plant cell is transformed with a construct encoding a
nucleic acid sequence to be excised, and the crossing is performed witha plant line containing a DSBI. As an alternative to the crossing step, a re-transformation of a transgenic plant line with a second construct can also be performed. The invention is also directed to a plant obtained by this method, or progeny, propagation material, part, tissue,
cell or
cell culture, derived from such a plant. Finally, the invention relates to the use of a plant or progeny, propagation material, part, tissue, cell or
cell culture, derived from this method, as aliment,
fodder or seeds or for the production of pharmaceuticals or chemicals.