The invention relates to a plant genomes deoxyribonucleic acid (DNA) quick extraction device and a testing method thereof. The plant genomes DNA quick extraction device is composed of a plurality of injectors with sponges on the bottoms, extraction pillars, and a plurality of centrifugal tubes, wherein the upper end portion of each of the extraction pillars can be connected with an injection port of each of the injectors in a sealing mode, the lower end portion of each of the extraction pillars can be connected with each of the centrifugal tubes, a loading seal cover is connected on the end portion of each of the extraction pillars, the loading seal cover is loaded with a silica film and is embedded in the inner side of the lower end portion of each of the extraction pillars in a clamping mode, and a through hole is formed in the center of the loading seal cover. According to the plant genomes DNA quick extraction device and the testing method thereof, compared with the prior art, large instruments of a centrifugal machine and the like are replaced by an air pressure method, the silica film is used for combining nucleic acid molecules, the plant genomes DNA quick extraction device is simple and effective, an extraction process is simple and convenient without any dependency of valuable instruments, testing time is short, the testing method is high in sensitivity, and the problem of quick testing of transgenetic products on site is resolved.