Method for using compound biological enzyme in plant extraction process, and conditions thereof

Abstract

The invention relates to a method for using compound biological enzyme in a plant extraction process, and conditions thereof. The compound biological enzyme comprises 5-60% of cellulose, 5-30% of hemicellulase, 5-50% of diastase, 5-50% of pectinase and 1-25% of protease, wherein the sum of the weight percentages of the components is 100%, and activity unit of the compound biological enzyme is 200000 U / g-1200000 U / g. The method for using the compound biological enzyme comprises: completely crushing a substrate until the substrate can passes a sieve having 80-200 meshes; adding the substrate to a water bath solution according to a ratio of material to liquid of 1:5-1:8; adjusting a pH value of the water bath solution to 4.0-5.0 through a acid (for example, acetic acid, oxalic acid, citric acid and the like); adding the compound biological enzyme to the water bath solution according to a certain ratio based on different substrates, followed by stirring completely and uniformly. A water bath temperature is controlled between 15-60 DEG C. The extraction time is controlled to 1-72 hours. The compound biological enzyme addition comprises the following conditions that: when the substrates are flowers, leaves or fruits of land plants, the compound biological enzyme addition is 0.1-0.5% of the substrate mass; when the substrates are roots and stems of the land plants, the compound biological enzyme addition is 0.3-0.8% of the substrate mass; when the substrates are marine plants, the compound biological enzyme addition is 0.8-3% of the substrate mass.

Description

technical field [0001] The invention relates to a method and conditions for a compound biological enzyme used in a plant extraction process. Background technique [0002] Most of the active ingredients contained in plants are wrapped in the cell wall. The traditional hot water, acid, alkali, and organic solvent extraction methods are often hindered by the cellulose in the cell wall, and the extraction time is often long, the amount of solvent is large, and the extraction efficiency is low. , and the impurities contained in the cells (such as protein, pectin, tannins, ash and sticky substances, etc.) also interfere with the extraction of active ingredients. At the same time, after the plants are treated with alcohol, their components may be changed or lost to varying degrees, so as to directly affect the pharmacological effects and curative effects of traditional Chinese medicine preparations. Therefore, the traditional extraction method not only increases the production cos...

AI Technical Summary

Problems solved by technology

[0002] Most of the active ingredients contained in plants are wrapped in the cell wall. The traditional hot water, acid, alkali, and organic solvent extraction methods are often hindered by the cellulose in the cell wall, and the extraction time is often long, the amount of solvent is large, and the extraction efficiency is low. , and the impurities contained in the cells (such as protein, pectin, tannin, ash and sticky substances, etc.) also interfere with the extraction of active ingredients

At the same time, after the plants are treated with alcohol, their components may be changed or lost to varying degrees, so as to directly affect the pharmacological effects and curative effects of traditional Chinese medicine preparations.

Therefore, the traditional extraction method not only increases the production cost, but also wastes precious plant resources.