Method for using compound biological enzyme in plant extraction process, and conditions thereof

A compound biological enzyme and plant extraction technology, applied in the field of plant extraction technology, can solve problems such as affecting the pharmacological action and curative effect of traditional Chinese medicine preparations, interfering with the extraction of active ingredients, wasting plant resources, etc., so as to maintain the original properties, reduce production costs, and improve The effect of extraction efficiency

Inactive Publication Date: 2011-11-16
WEIHAI KANGBOER BIOLOGICAL PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Most of the active ingredients contained in plants are wrapped in the cell wall. The traditional hot water, acid, alkali, and organic solvent extraction methods are often hindered by the cellulose in the cell wall, and the extraction time is often long, the amount of solvent is large, and the extraction efficiency is low. , and the impurities contained in the cells (such as protein, pectin, tannin, ash and sticky substances, etc.) also interfere with the extraction of active ingredients
At the same time, after the plants are treated with alcohol, their components may be changed or lost to varying degrees, so as to directly affect the pharmacological effects and curative effects of traditional Chinese medicine preparations.
Therefore, the traditional extraction method not only increases the production cost, but also wastes precious plant resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Extraction of Flos Lonicerae by using complex biological enzymes.

[0021] (1) Take 2000g of dried honeysuckle, and ultrafinely pulverize it so that it can pass through a 100-mesh sieve.

[0022] (2) Get 4.5g of cellulase, 2.0g of hemicellulase, 1.5g of amylase, 1.5g of pectinase, and 0.5g of protease to mix into a 10g heavy composite biological enzyme, and the activity unit of the enzyme is 500,000 U / g .

[0023] (3) Add 2000g of crushed honeysuckle fine powder to 6 times the amount of water, then raise the temperature to 48°C, add 10g of pre-configured compound biological enzyme solution, adjust the pH value to 4.5 with oxalic acid, stir well and keep at 48°C Enzyme hydrolysis for 60 minutes, then heated up to 100°C for rapid inactivation, then kept at the temperature for 1 hour of leaching, and then cooled down naturally.

[0024] (4) Filtration, deslagging, concentration, and drying to obtain the extract.

[0025] (5) Simultaneously as a comparison, ta...

Embodiment 2

[0027] Example 2: Extracting Coptidis rhizome with biological complex enzyme.

[0028] (1) Take 2000g of Coptidis Rhizoma, and ultrafinely pulverize it so that it can pass through a 200-mesh sieve.

[0029] (2) Take 7.0g of cellulase, 3.0g of hemicellulase, 2.0g of amylase, 1.0g of pectinase, and 1.0g of protease and mix them into a 14g heavy compound biological enzyme. The activity unit of the enzyme is 400,000 U / g .

[0030] (3) Add 2000g of pulverized Coptis chinensis fine powder to 3 times the amount of water, add 14g of pre-configured compound biological enzyme solution, adjust the pH value to 5 with 0.3% sulfuric acid, soak it, and keep it in a constant temperature water bath for 90 minutes at 40°C. Coptis chinensis and Put 0.3% sulfuric acid as a solvent in the percolation cylinder, soak and percolate, collect the percolation liquid, adjust the pH value to 10-12 with lime milk, precipitate, filter with suction, adjust the pH value of the filtrate to 1-2 with concentrat...

Embodiment 3

[0033] Example 3: Extraction of seaweed by biological compound enzyme.

[0034] (1) Take 1000 g of dried seaweed, and ultrafinely pulverize it so that it can pass through a 200-mesh sieve.

[0035] (2) Get 10.5g of cellulase, 2.5g of hemicellulase, 2.5g of amylase, 3.0g of pectinase, and 1.5g of protease to mix into a 20g heavy compound biological enzyme, and the activity unit of the enzyme is 700,000 U / g .

[0036] (3) Add 8 times the amount of water to 1000g of crushed seaweed fine powder, then raise the temperature to 50°C, add 20g of pre-configured compound biological enzyme solution, adjust the pH value to 4.5, stir well and keep 50°C for constant temperature enzymolysis 2h. Then rapidly raise the temperature to 90°C for rapid inactivation, inactivate for 1 minute, then cool down to 80°C for 1 hour of extraction.

[0037] (4) Filtration, deslagging, concentration, and drying to obtain the extract.

[0038] (5) Simultaneously as a control, take 1000 g of dried seaweed,...

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PUM

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Abstract

The invention relates to a method for using compound biological enzyme in a plant extraction process, and conditions thereof. The compound biological enzyme comprises 5-60% of cellulose, 5-30% of hemicellulase, 5-50% of diastase, 5-50% of pectinase and 1-25% of protease, wherein the sum of the weight percentages of the components is 100%, and activity unit of the compound biological enzyme is 200000 U/g-1200000 U/g. The method for using the compound biological enzyme comprises: completely crushing a substrate until the substrate can passes a sieve having 80-200 meshes; adding the substrate to a water bath solution according to a ratio of material to liquid of 1:5-1:8; adjusting a pH value of the water bath solution to 4.0-5.0 through a acid (for example, acetic acid, oxalic acid, citric acid and the like); adding the compound biological enzyme to the water bath solution according to a certain ratio based on different substrates, followed by stirring completely and uniformly. A water bath temperature is controlled between 15-60 DEG C. The extraction time is controlled to 1-72 hours. The compound biological enzyme addition comprises the following conditions that: when the substrates are flowers, leaves or fruits of land plants, the compound biological enzyme addition is 0.1-0.5% of the substrate mass; when the substrates are roots and stems of the land plants, the compound biological enzyme addition is 0.3-0.8% of the substrate mass; when the substrates are marine plants, the compound biological enzyme addition is 0.8-3% of the substrate mass.

Description

technical field [0001] The invention relates to a method and conditions for a compound biological enzyme used in a plant extraction process. Background technique [0002] Most of the active ingredients contained in plants are wrapped in the cell wall. The traditional hot water, acid, alkali, and organic solvent extraction methods are often hindered by the cellulose in the cell wall, and the extraction time is often long, the amount of solvent is large, and the extraction efficiency is low. , and the impurities contained in the cells (such as protein, pectin, tannins, ash and sticky substances, etc.) also interfere with the extraction of active ingredients. At the same time, after the plants are treated with alcohol, their components may be changed or lost to varying degrees, so as to directly affect the pharmacological effects and curative effects of traditional Chinese medicine preparations. Therefore, the traditional extraction method not only increases the production cos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12N9/42C12N9/26C07C69/732C07C67/58C07D455/03
Inventor 于国友王佃亮殷龙民
Owner WEIHAI KANGBOER BIOLOGICAL PHARMA
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