130 results about "Diastase" patented technology

The invention relates to a method for jointly treating stalks by steam explosion and microorganism fermentation, comprising the following steps of: firstly, carrying out steam explosion pretreatment on the stalks to obtain exploded stalks; then preparing spore seed liquid: inoculating aspergillus oryzae or trichoderma Koningi to a PDA (Potato Dextrose Agar) culture medium, standing and culturing for 3-5 days at the temperature of 28-32 DEG C, and preparing the spore seed liquid with the concentration of 106-108 per mL; and finally carrying out microorganism fermentation: uniformly mixing 18g of exploded stalks, 2g of bran and 30mL of mineral element nutrient solution, adjusting pH to be 7.0 with Ca(OH)2, sterilizing for 15min at the temperature of 121 DEG C and the pressure of 0.15MPa to obtain a solid fermentation medium a, inoculating the spore seed liquid according to 2-4% of inoculum size, and culturing the spore seed liquid for 5-7 days at the temperature of 28-32 DEG C. The fermentation stalks obtained by utilizing the method have low content of lignose, cellulose and hemicellulose and high activity of filter paper carbohydrase, CMC (carboxymethyl cellulose) enzyme, amylase and protease.

The invention relates to a biological Increasing the quality agent of tobacco and its preparation method and application. the Increasing the quality agent comprises (by weight parts) Bacillus megaterium ZXB1 (CGMCC 1.941) 180-200, Bacillus subtilis subsp. Subtilis (CGMCC 1.354) 180-200, diastase 5-15, amylase 50-200, and protease 450-550. The invention has the advantages of improved product fermentation quality, shortened aging period by 10-12 months, improved chemical ingredients in tobacco, improved tobacco flavor, reduced cost by 3.5 yuan for each box, and improved tobacco grade by one third.

The invention discloses a method for preparing fructose syrup. The method includes the steps: preparing starch milk, adding alpha-diastase, performing spray liquefaction and hydrolysis reaction, flashing reaction liquid to obtain liquefied liquid and collecting waste heat of flash steam; adding glucose saccharifying enzyme into the liquefied liquid, sequentially filtering saccharified materials by organic membranes, desalting the materials by means of ion exchange and evaporating and concentrating the materials to obtain reaction liquid with the refractive index of 38-45%; and isomerizing the reaction liquid by glucose isomerase, desalting the reaction liquid by means of ion exchange, discoloring the reaction liquid and evaporating and concentrating the reaction liquid by means of MVR (mechanical vapor recompression) to obtain the fructose syrup. Heat for evaporation and concentration is provided by the waste heat of the flash steam. The organic membranes are used for deproteinization and filtration, one time of discoloration is decreased, consumption of activated carbon is reduced, and the service cost of the fructose syrup is saved. The heat needed for one-time evaporation and concentration is provided by the waste heat of the flash steam, live steam is not consumed, total energy consumption is reduced, and invested production cost is further reduced.

The invention relates to a health-care red koji wine and a production process thereof. The wine comprises the following raw materials in percentage by weight: 11.5-12.5% of nonglutinous rice, 13-17% of sticky rice, 4.5-5.5% of Chinese herbal medicine, 4.5*10<-2> to 5.5*10<-2>% of yellow wine herbal medicine starter and white rhizopus medical powder, 1-2% of red yeast rice, 4.5*10<-3> to 6.5*10<-3>% of diastase, 1*10<-3> to 1.5*10<-3>% of acid protease and 65-68% of water, wherein the using amount ratio of the yellow wine herbal medicine starter to the white rhizopus medical powder is (1-2):1.The production process provided by the invention comprises the following working procedures of preparing raw materials; preparing a Lin-fan rice wine starter; primarily pickling the sticky rice; secondarily pickling the Chinese herbal medicine and the sticky rice; carrying out post-fermentation at low temperature; squeezing and filtering; decocting the wine and sterilizing; storing and ageing; blending; freezing and flocculating; filtering and filling, and the like; and the prepared health-care red koji wine has low alcoholic strength, salubrious, savoury and mellow taste, and has the effectsof tonifying Yang and replenishing the vital essence, enforcing spleen and nourishing kidney as well as bodybuilding and healthcare.

The invention provides an application of application of plant alpha-diastase in pollen abortion, and belongs to the technical field of genetic engineering. Through introducing polynucleotide of the plant alpha-diastase, recombinant vector and recombinant vector are structured; a pollen specific promoter drives the plant alpha-diastase to degrade starch in advance during the pollen developmental phase; the pollen sprouting energy cannot be provided, and the pollen sprouting is contained, thus abortion is generated; the transgenetic seedling of transgene pollen abortion is obtained. The plant alpha-diastase can be used as the pollen abortion gene and can be originated from genes of rice endogenesis, millet, broomcorn, barley, wheat and other gramineous plants; the plant alpha-diastase is very good for transgenic plant of pollen abortion. The plant alpha-diastase gene has accurate expression level and can control the spreading of transgenosis; the plant alpha-diastase gene also can keep and breed a male sterility line while leave out the artificial castration step during the hybrid seed production process; the application prospect is wide.

The invention discloses a dyeing and finishing process of polyester and cotton blended fiber fabric. The process comprises pretreatment, presetting, modification treatment, dyeing, mordant removal, soaping, color fixing, after finishing, drying, sizing, calendaring and rolling. Snailase and alpha-amylase are adopted for pretreatment, modification treatment is adopted before dyeing, the polyester and cotton blended fabric is modified fundamentally, and the dye-uptake of the fabric is increased; in the dyeing procedure, fewer alkaline substances and inorganic salt are used, a dye leveler, substitution alkali, a migration inhibitor and a diffusant are added, the dye leveling performance is improved, natural dye is used, environmental pollution is avoided, and the environment-friendly requirement is met; self-cleaning treatment is added during aftertreatment, and the self-cleaning capacity, the crease-resistant performance, the wash durability and color fastness are improved; silk fibroin and sericin are adopted for afterfinish, and the anti-pilling performance and the antistatic performance of the fabric are provided; the whole process is environment-friendly and pollution-free.

Provided is a preparation method of a crosslinking-enzymolysis composite superfine modified starch adsorbent. The method comprises the steps that 1, corn, sweet potatoes and potatoes are superfinely ground into superfine starch with the granule size of 10-50 micrometers through a superfine grinder separately; 2, the three kinds of superfine starch are mixed to be uniform according to the mass ratio of 1:4:3, water is added into the materials, stirring is conducted, and starch emulsion is obtained; 3, the pH of the starch emulsion is adjusted to be 7.5-8.5, a certain amount of sodium tripolyphosphate solution is added, and crosslinking is conducted for 1-1.5 h at the temperature of 75 DEG C-80 DEG C; 4, centrifugal dewatering is conducted on crosslinked starch emulsion, the dewatered starch emulsion is washed with deionized water, and freeze drying is conducted; 5, the crosslinked starch is added with water and blended into starch emulsion again, the pH is adjusted to be 6.5-7.5, alpha-amylase and isoamylase are added, and an enzymolysis reaction is conducted for 12-15 h at the temperature of 50 DEG C; 6, the starch emulsion subjected to enzymolysis is centrifuged, washed and dried and then sieved through a 40-100-mesh sieve, and the crosslinking-enzymolysis composite superfine modified starch is obtained. The modified starch significantly increases the specific surface area of porous starch, improves the mechanical strength of the porous starch and improves the adsorption capacity of the porous starch on harmful ingredients, especially, heavy metal elements.

The present invention relates to a method for preparing Chinese herbal medicine by using enzyme preparation. It is extracted by utilizing biochemical action. It is characterized by that it utilizes catalystic reaction of enzyme and fermentation action of microorganism, according to different medicinal material composition said invention selects the multienzyme body containing pectic enzyme, alpha-diastase and proteinase as enzyme preparation and utilizes said enzyme preparation to make the Chinese herbal medicine undergo the sufficient enzymolysis and fermentation so as to obtain the invented Chinese herbal medicine product.

The invention discloses an environmental-friendly dyeing and finishing process of a real silk fabric. The environmental-friendly dyeing and finishing process of the real silk fabric comprises pretreatment, presetting, cryogenic freezing treatment, dyeing, mordant removal, soaping, color fixing, post-finishing, drying, setting, calendering and winding. By performing pretreatment with helicase and alpha-amylase and by adopting cryogenic freezing treatment before dyeing, the performance of the real silk fabric is fundamentally modified and the dye uptake rate of the fabric is improved; a great amount of alkali substances and inorganic salt are not used in the dyeing process and leveling agent and dispersing agent are added, the dyeing uniformity is improved, the environmental pollution is avoided by using natural dyes and the requirements on the environmental protection are satisfied; since self-cleaning treatment is added during post-treatment, the self-cleaning ability, the wrinkling resistance and the water washing fastness of the fabric are improved; the entire process is environmental-friendly and pollution-free.

The invention relates to the field of food processing, in particular relates to a whole grain vegetable sausage and a preparation method thereof, and aims to solve the technical problems that whole grain food cannot be made into convenient food and cannot be popularized for eating because whole grains are rich in starch which is likely to retrograde. Raw materials of the whole grain vegetable sausage comprise soy protein, salad oil, plain white, starch, water, composite gel, sticky rice, sweet corn grit, sesame, red bean sugar natto, green bean sugar natto, sorghum, soybean, oatmeal and the like. The preparation method comprises the following steps of: (a), raw material treatment; (b), addition of diastase; (c), cutting and blending; (d), filling and tying; (e), sterilization; (f), cooling. The invention provides a new method for preparation of a vegetable product. The product tastes good, the starch is unlikely to retrograde, the safety of food shelf life is greatly improved, and the whole grain vegetable sausage is wide in application prospect.

The present invention discloses a production method of concentrated barley juice. Said production method uses barley grains as raw material and includes the following steps: selecting grains of barley, peeling, pulverizing and pulping, making prehydrolysis, jetting to make continuous liquification, two-stage saccharification, filtration, concentration and canning. It is characterized by that in the prehydrolysis procedure a composite hydrolase formed from high-temperature resistant alpha-diastase, proteinase and beta-glucanase is added, in the first-stage saccharification procedure a composite hydrolase composed of medium-temperature alpha-diastase, proteinase, beta-glucanase, xylanase and phytase is added and in the second-stage saccharification procedure a composite hydrolase formed from beta-diastase and phytase is added.

The invention provides a low sugar low alcohol health preserving yellow rice wine, which comprises raw materials by weight percentage: 12-15% of polished round-grained rice, 10-15% of white glutinous rice, 4-8% of black glutinous rice with peel, 4x10<-2>%-7x10<-2>% of yellow rice wine yeast, raw wine yeast 1.5-2.5%, 1-2% of red kojic rice, 2.5x10<-3>%-4x10<-3>% of diastase, 1x10<-3>%-2.5x10<-3>% of acidic protease, and 60-65% of water. Based on the weight of clear wine obtained after squeezing and filtering, the wine also comprises 5.7-6.9% of food and medicine plant materials. The invention has the advantages of full bodied wine, improved function, low alcohol, low sugar, coordinated mouthfeel with nutrition and health function.

This invention relates to a method for preparing lactic acid from food garbage by saccharification with Bacillus subtilus. The method solves the problem of high cost by adding amylase or cellulose into food garbage to prepare lactic acid. The method comprises: (1) filtering food garbage, drying, and pulverizing; (2) adding Bacillus subtilus; (3) saccharifying; (4) adding water; (5) adding lactic acid bacteria, adding calcium carbonate, and fermenting; (6) filtering liquid and solid, and purifying lactic acid in the filtrate to obtain the final product. The method does not need high-temperature sterilization. The yield of lactic acid is 50-55.7g lactic acid/L solid-liquid-mixed food garbage, and the content of lactic acid content in organic acids in the fermentation liquid is 94-97 wt.%. Besides, the cost is saved by 20-26% compared with that of adding diastase or cellulase.

The invention relates to a preparation method of a liquid Beta-diastase, which is characterized by taking wheat bran as raw material. The preparation method comprises the following technological steps: the extraction of the wheat bran, solid-liquid separation, the fine filtration of an extract, ultra-filtration concentration, prescription, secondary fine filtration and the adjustment of enzyme activity. The preparation method adopts a preservative with proper matching and proper temperature and Ph so as to effectively inhibit bacterial growth and avoid putrefaction and enzyme deactivation; the obtained products have low content of impurity, contains low concentrate of industrial salt compared with that of a solid enzyme preparation and meets the requirements of General Standard for Food Additives easily. The preparation method of the invention is a brand new preparation technology of the liquid Beta-diastase and has the advantages of simple technology, high production efficiency, low production cost and being suitable for industrialized production.

The invention relates to a method for preparing low-digestible energy (DE) value maltodextrin. The method comprises the following steps of: (1) adding cereal starch serving as a raw material into water to prepare starch, regulating the concentration of a dried substance of the starch to be 20 to 30 percent, regulating the ph value to be 5.8 to 6.2, and adding high temperature-resistant diastase into the starch; and (2) steaming the starch by using a digester at the temperature of between 108 and 110 DEG C for 5 to 6 minutes to obtain starch paste, liquefying and hydrolyzing the starch paste for 30 to 150 minutes, heating and killing enzyme to obtain a maltodextrin aqueous solution. By the method, the low-DE value maltodextrin can be obtained stably, is in non-blue color by iodine tests and is not needed to be filtered; and by the method, the good economic and reliable condition for producing the low-DE value maltodextrin is created, and the method has a good application prospect.

The invention discloses maize germ instant powder containing xylooligosaccharide. The maize germ instant powder is prepared by the following method: uniformly mixing 60-80% of maize germ meal with 2-4% of xylooligosaccharide, 6-12% of xylitol and 12-25% of skim milk powder, and sterilizing to obtain the maize germ instant powder containing xylooligosaccharide. According to the maize germ instant powder containing xylooligosaccharide, which is disclosed by the invention, the maize germ meal is compounded with the xylooligosaccharide to greatly increase the absorption rate of protein in the maize germ and remedy the defect of low absorption rate of plant protein, and meanwhile, the xylooligosaccharide also has the functions of promoting gastrointestinal peristalsis, proliferating beneficial intestinal bacteria and relaxing bowels. The maize germ instant powder is hydrolyzed by papain to increase the content of soluble protein and enable the xylooligosaccharide to well play the function of prompting protein absorption; the maize germ instant powder is hydrolyzed by alpha-amylase and cellulose to improve the absorption of soluble sugar, improve the solubility of the corn germ meal, reduce the agglomeration rate of the corn germ meal and perfect the cooking taste of the maize germ meal.

The invention discloses a preparation method of jasmine flower and white tea turbid craft beer, and belongs to the technical field of preparation methods of craft beer. The method comprises the following steps of crushing, saccharifying and filtering malt to obtain saccharified wort; boiling the wort, adding hops, white tea and jasmine flowers, and performing solid-liquid separation on the wort containing the hops, the white tea and the jasmine flowers to obtain jasmine flower and white tea wort; carrying out primary fermentation, adding the white tea for the second time, and continuing to perform fermentation to obtain a fermented mixture; and after discharging of yeast, sequentially carrying out filling, secondary fermentation in a bottle and low-temperature wine storage. According to the craft beer prepared by the method, polyphenol, amino acid components and tea fragrance of the white tea are reserved to a great extent; the beer is endowed with special flower fragrance by adding the jasmine flowers, so that the taste of the craft beer is enriched; the prepared tea beer is stable in turbidity and color and lasting in foam; and the tea polyphenol can enable the tea beer to have the effect of reducing blood sugar by inhibiting the activity of alpha-amylase and alpha-glucosidase.

The invention discloses a preparation method of an astaxanthin biological feed, which comprises the following steps of: dissolving manioc waste and preparing manioc waste liquid, adding cellulase and diastase after high-temperature sterilization for enzymolysis, and then adding ammonium sulphate, monopotassium phosphate, yeast extract and tomato juice to the manioc waste liquid; inoculating third class seed liquid of Phaffiarhodozyma after high-temperature sterilization for fermentation, biosynthesizing astaxanthin to obtain astaxanthin fermentation liquor, then utilizing ultrasonic wave to break Phaffiarhodozyma cell, drying at the temperature of 45 to 60 DEG C, vacuumizing and sealing, storing at low temperature, and obtaining the astaxanthin biological feed. The preparation method has simple technique; and the obtained astaxanthin biological feed has high astaxanthin content and protein content, is easy to be digested and absorbed by animals, does not contain alcohol substance, has good palatability, and does not contain toxic substance, thus improving the immunity of animals for aquatic product and coloring strength of meat, and being a high-quality feed with the function of health protection.

The invention relates to a preparation method for wheat bran dietary fiber. The preparation method comprises the following steps: (1) crushing the wheat bran, soaking the crushed wheat bran and defibrinating; (2) adjusting the pH value of a material to be 7.5-9.0 and adding alkaline protease into the material and reacting for 2.0-3.0 hours; (3) heating the material to 65-75 DEG C, adding mesophilic alpha-diastase and reacting for 30-80 minutes; (4) cooling the material to 45-55 DEG C, adding phytase and reacting for 2.0-3.0 hours; and (5) carrying out suction filtration in vacuum on the material and drying, puffing, roasting and crushing the residues after suction filtration to obtain the wheat bran dietary fiber. The obtained wheat bran dietary fiber product not only is low in content of phytic acid, but also has relatively strong water absorption capability. Other components in the bran are further fully utilized. The used reagents are free of toxicity and harm, so that the problem of environmental pollution is avoided, and moreover, the economical benefit of the product is improved.

The invention discloses a preparation method of a toona sinensis extract. The preparation method comprises the steps that 1, a toona sinensis material is taken to be subjected to ultrasonic-assisted extraction in ethyl alcohol; 2, filter liquid is taken to evaporate ethyl alcohol, so that filter residue is obtained; 3, the filter residue is centrifuged, so that a toona sinensis crude extract is obtained, and distilled water is added to dilute the toona sinensis crude extract into toona sinensis extract diluted liquid for stand-by application; and 4, the toona sinensis extract diluted liquid ispurified. The toona sinensis extract prepared by adopting the preparation method, particularly a toona sinensis flower extract, can effectively reduce the activity of alpha-diastase and alpha-glucosidase in the body, reduces the blood sugar content in the body, and accordingly is the optical selection for preparing drugs or health care products for treating or relieving diabetes.

The invention discloses a method for improving output of tetraene macrolide antibiotics and belongs to the technical field of microorganisms. According to the method, 100mu g/mL paromomycin is added in a solid medium which is used for culturing Streptomyces diastatochromogenes D to obtain a Streptomyces diastatochromogenes mutant strain which has relatively good resistance to the paromomycin; the capacity of synthesizing the tetraene macrolide antibiotics of the mutant strain is stronger than that of the Streptomyces diastatochromogenes D.

The invention discloses a method of extracting Alpha amylase inhibitor from kidney beans. The method is as follows: the crushed kidney beans are first dispersed in a buffer solution with a definite pH value, and then shifted into an ultrasonic device for ultrasonic extraction, and then conducted enzyme elimination, centrifugalization, dialysis and ethanol sediment, dried and finally the Alpha amylase inhibitor is obtained. The Alpha amylase inhibitor extracted from kidney beans in the invention is a white solid powder with a protein content of 80 to 95 percent and an inhibitory activity of 5,000 to 50,000 U/g. Compared with the prior method of lixiviation and extraction, the method of extracting Alpha amylase inhibitor from kidney beans has the characteristics of high speed, high efficiency, energy conservation and small pollution. The one-time extracted Alpha amylase inhibitor has high content and strong activity, thereby avoiding the fact that circulative extractions consume a large quantity of water and energy. The method of extracting Alpha amylase inhibitor from kidney beans is a novel green environment-friendly technology with a wide range of application prospects.

The invention discloses an S.diastatochromogenes electroporation transformation method, which comprises: carrying out a mild wall breaking treatment on cells, and carrying out electroporation transformation, wherein the mild wall breaking treatment adopts an electroporation buffer solution containing 5 mug/ml of lysozyme, or adopts an electroporation buffer solution containing 1% glycine, or adopts an electroporation buffer solution containing 30 mug/ml of penicillinG, or adopts an electroporation buffer solution containing 3 mug/ml of lysozyme and 20 mug/ml of penicillinG, and the electroporation transformation parameters comprise an electric field intensity of 12 kV/cm, a cell concentration of 1*10<9>/ml, and a plasmid DNA concentration of 2 mug; and adopting a YEME culture medium to incubate after electroporation transformation, wherein an incubation time is 2-3 h. According to the present invention, a convenient and reliable method is provided for efficient transformation of S.diastatochromogenes, and a path is paved for S.diastatochromogenes gene engineering.

The invention relates to a method for extracting giantarum polysaccharides, which comprises the following steps of: cleaning giantarum used as raw material, and then freezing; slicing the frozen giantarum, adding a right amount of water for pulping, centrifuging, and concentrating the supernate; adding diastase to remove starch, filter-pressing, and adding absolute alcohol till the alcohol concentration reaches 80-85%; dissolving the precipitate with water, precipitating with alcohol 1-3 times, and freezing and drying the precipitate to obtain the giantarum polysaccharides. The extraction method is simple, rapid, safe and free of pollution and has high yield and low cost. The content of giantarum polysaccharides in the product is not less than 70%.