65 results about "Diastase" patented technology

The invention relates to a preparation method and application of sea buckthorn pomace flavone extract freeze-dried powder, and belongs to the technical field of natural product extraction. The method comprises the following steps: pretreating (drying and powdering) a sea buckthorn pomace raw material, preparing a crude extract, purifying the extract, freeze-drying the extract, and finally obtaining the sea buckthorn pomace flavone extract freeze-dried powder. The by-product sea buckthorn pomace in the sea buckthorn processing process is subjected to resource utilization, the vibration type ultrafine pulverizer is adopted for pulverizing to increase the extraction surface area, the ultrasonic synergistic biological enzymolysis method is adopted for promoting release of active molecules, the efficiency is improved, energy and time are saved, no harmful solvent is used in the extraction process, and the method is environmentally friendly, simple in preparation process and high in operability; the large-scale production is easy to realize. According to the sea buckthorn pomace flavone extract freeze-dried powder prepared by the method, the total flavone extraction amount reaches 17.79-23.05 mg / g of the dry weight of sea buckthorn pomace, the purity can reach 50-65%, and the sea buckthorn pomace flavone extract freeze-dried powder has good oxidation resistance, alpha-glucosidase inhibitory activity and alpha-amylase inhibitory activity and can be used for developing products for reducing blood sugar.

The invention relates to the technical field of functional cosmetic raw materials, in particular to a cactus polysaccharide composition as well as a preparation method and application thereof. According to the cactus polysaccharide composition disclosed by the invention, the cactus polysaccharides with specific number-average molecular weight are combined according to a specific proportion, so that the cactus polysaccharide composition has a more comprehensive anti-irritation effect and has the characteristics of high stability and better skin feeling and solubility. The cactus polysaccharide can be prepared through a simple process, exogenous components do not need to be introduced, after the cactus aqueous extract is subjected to enzymolysis through amylase, the cactus polysaccharide with the specific number-average molecular weight is screened and combined according to the specific proportion, the anti-irritation effect requirement can be met, and safety is better. The cactus polysaccharide composition can be applied to cosmetics / pharmaceuticals as an anti-irritation raw material.

The method for preparing D-mannose through catalysis can effectively reduce the amount of by-products glucose and fructose, and greatly improves the conversion rate of D-mannose. Specifically, engineering bacteria for expressing isoamylase genes and engineering bacteria for expressing alpha-glucan phosphorylase genes, glucophosphate mutase genes, difunctional enzyme glucophosphate isomerase / mannose 6-phosphate isomerase genes and mannose 6-phosphate phosphatase genes are utilized, starch or starch derivatives are used as substrates, and the starch or starch derivatives are used as substrates. A phosphate buffer solution and Mg < 2 + > are added, a preliminary catalysis system is constructed, after the reaction is completed, an incompletely-reacted substrate and maltodisaccharide exist in the reaction system, the incompletely-reacted substrate and maltodisaccharide can be hydrolyzed into glucose by glucoamylase, and the polyphosphoglucokinase can be used for catalyzing the reaction of the polyphosphoglucokinase under the assistance of sodium hexametaphosphate. All glucose is converted into phosphorylated glucose, then D-mannose is generated, and by-products in a system are removed while the yield is increased.

The present invention provides a chemical synthesis method of EPS-G7, wherein β-cyclodextrin A is acetylated to obtain β-acetylcyclodextrin B, B is ring-opened in a mixed solution of acetic anhydride / concentrated sulfuric acid to obtain a chain acetylated heptose C, p-nitrophenol is condensed with intermediate C using boron trifluoride acetonitrile to obtain a key intermediate p-nitrophenyl (G1)-O-acetyl-D-maltoseptaglycoside D with α as the dominant configuration, D is removed from the acetyl group under the action of sodium methoxide to obtain pNP-G7 (p-nitrophenyl (G1)-D-maltoseptaglycoside), pNP-G7 is in the presence of p-toluenesulfonic acid and dimethoxyethane, and acetal protection is formed between the hydroxyl groups on C4 and C6 of the terminal monosaccharide to obtain EPS-G7. The reaction raw materials used in the method of the present invention are cheap and readily available, and the chemical synthesis of α-amylase detection reagent EPS-G7 is realized, which is a simple, economical and efficient synthesis method.

The invention aims to provide a method for improving nutritional quality of germinated brown rice through ultrasonic-assisted salt induction. Brown rice is a whole-grain product and is rich in nutritional ingredients such as starch, protein, dietary fibers, vitamins and mineral substances and active functional factors. The nutritional ingredients of the germinated brown rice change drastically, and the nutritional value of the brown rice is greatly improved. According to the method, brown rice is taken as a raw material, the brown rice is soaked in a CaCl2 salt solution under the assistance of ultrasound, the germination temperature and time are optimized, the concentration of the CaCl2 salt solution is taken as an influence factor, and the change rule of nutritional ingredients and functional factors of the brown rice in the germination process is researched. Results show that after ultrasonic-assisted salt induction treatment, the contents of vitamin B2 and vitamin B6 in the germinated brown rice are increased to 58.2 mu g / 100g and 0.438 mg / 100g, and the content of gamma-aminobutyric acid is increased to 42.6 mg / 100g (dry basis). The germination of the brown rice promotes the activation and release of hydrolases such as amylase, protease and phytase, and nutritional ingredients in the brown rice are migrated, degraded, accumulated and the like, so that the nutritional quality of the brown rice is remarkably improved.

The invention provides a preparation method of spleen-tonifying and stomach-nourishing granules, which comprises the following steps: step 1, crushing fructus amomi into fine powder, and sieving for later use; step 2, adding water into the dried orange peel, extracting volatile oil by adopting a steam distillation method, separating the dried orange peel volatile oil from dried orange peel residues, drying the dried orange peel residues, adding ethanol which submerges the dried orange peel residues, standing, percolating in a percolation device, adding ethanol into the percolation device for soaking, collecting percolate, and concentrating the collected percolate under reduced pressure to obtain the dried orange peel extract. Adding pericarpium citri reticulatae volatile oil, and uniformly mixing to obtain pericarpium citri reticulatae clear paste; step 3, adding water into codonopsis pilosula, polyporus umbellatus, poria cocos and liquorice, decocting twice, filtering, combining decoction, and concentrating into first clear paste; step 4, performing reflux extraction on the officinal magnolia bark, the green tangerine peel and the largehead atractylodes rhizome through ethanol, recovering the ethanol until no ethanol smell exists, and performing vacuum concentration to obtain second clear paste; step five, adding starch into the dried orange peel clear paste, the first clear paste and the second clear paste, uniformly stirring to prepare large particles, and drying; and 6, adding yeast powder, amylase, fructus amomi powder and a sweetening agent into the dried product obtained in the step 5, crushing into fine powder, uniformly mixing, preparing into granules by using ethanol as a wetting agent, drying, and subpackaging into 1.5 g / bag. Effective components of the components in the prescription can be extracted to the greatest extent, so that the medicine effect is improved, and the problem that the dosage is difficult to accurately grasp when children take the medicine is solved.

The invention discloses an alpha-1, 4 glucosidic bond hydrolytic ability enhanced oligomerized 1, 6-glucosidase mutant and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. The oligomerized 1, 6-glucosidase mutant V219A / P227A disclosed by the invention also has the alpha-1, 4 glucosidic bond hydrolytic activity which is not possessed by a wild type enzyme on the basis of retaining the original alpha-1, 6 glucosidic bond hydrolytic activity, and the alpha-1, 4 glucosidic bond hydrolytic activity is improved by 267% compared with that of a single mutant V219A. The mutant V219A / P227A is applied to an enzymatic production process of glucose, maltodextrin is taken as a substrate, glucamylase and the mutant V219A / P227A are added for enzymatic conversion, and the final glucose yield reaches 98.0%; the mutant V219A / P227A is applied to regeneration of glucose from crystalline glucose mother liquor, and the final glucose yield reaches 95.9%, which is 9.35% and 5.15% higher than those of a wild type V219A and a single mutant V219A respectively. Therefore, the mutant is helpful for improving the substrate conversion rate and the product purity in the glucose production process, can be used for regenerating glucose from crystalline glucose mother liquor, and has higher industrial application value.

The invention relates to the technical field of aquaculture feed, and provides a compound feed additive for improving fish sugar utilization rate and promoting growth, the compound feed additive comprises the following raw materials by weight: 0.1-0.14 part of chromium picolinate, 1.5-2.5 parts of vitamin C, 20-40 parts of amylase, 20-40 parts of protease, 10-20 parts of dimethyl-beta-propiothetin, 2-4 parts of bile acid, and 18-22 parts of a carrier. The compound feed additive provided by the invention has the beneficial effects that the blood sugar is regulated, and the sugar metabolism capability of fish bodies is improved; due to the use of the compound feed additive, energy supply caused by protein decomposition is reduced, so that the growth performance of the fishes and the utilization rate of the feed are improved; the low-temperature stress resistance of the fishes is improved, and the breeding negative influence caused by temperature change is reduced; the protein loss is reduced; the feed cost is reduced; and the culture benefit is improved.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The invention discloses a stenotrophomonas sp. A322 strain and an application of the stenotrophomonas sp. A322 strain in synthesis of acarbose. The stenotrophomonas sp. A322 is preserved in the China Center for Type Culture Collection, and the preservation number of the stenotrophomonas sp. A322 is CCTCC (China Center for Type Culture Collection) NO: M 2025917. The stenotrophomonas sp. A322 can be used for synthesizing acarbose by using maltose and glucose as carbon sources, and the acarbose can be synthesized by using the stenotrophomonas sp. The stenotrophomonas sp. A322 is subjected to fermentation culture in a 100 mL shake flask fermentation system for 1 to 7 days, and the acarbose yield is 0.4 to 4 g / L. The high performance liquid chromatography retention time and the nuclear magnetic resonance carbon spectrum chemical shift of the acarbose obtained by fermentation, separation and purification of the Stenotrophomonas sp. A322 are consistent with those of a standard substance, and the high performance liquid chromatography retention time and the nuclear magnetic resonance carbon spectrum chemical shift of the acarbose obtained by fermentation, separation and purification of the Stenotrophomonas sp. A322 are consistent with those of the standard substance. Activity detection shows that the separated and purified acarbose sample has alpha-amylase inhibitory activity.

The invention discloses a denitrification and dephosphorization agent and a preparation method thereof. First, corn stalks and a metal nitrate aqueous solution are used as raw materials to prepare metal-based biochar, and then acrylamide is polymerized in situ with the metal-based biochar as a matrix to prepare modified metal-based biochar; then acrylic acid is used to graft starch to obtain acrylic acid grafted starch, and then the acrylic acid grafted starch is reacted with ethylenediamine solution to obtain amino chelated starch, and enzymatic hydrolysis is performed to obtain porous amino chelated starch; finally, the modified metal-based biochar and the porous amino chelated starch are compounded to obtain the agent. The agent can simultaneously achieve denitrification and dephosphorization of sewage, and has very good application prospects.

The application belongs to the technical field of medicine, and relates to a compound composition, a preparation method and application, in particular to a compound sodium salicylate composition for treating acute pancreatitis and a preparation method. The compound sodium salicylate composition comprises quercetin, diammonium glycyrrhizinate and sodium salicylate, wherein the mass ratio of quercetin, diammonium glycyrrhizinate and sodium salicylate is 1-5:5-20:50-200. When the compound sodium salicylate composition is an injection, it comprises quercetin, diammonium glycyrrhizinate, sodium salicylate, 1,2-propanediol, sodium bisulfite, dimethyl sulfoxide and 0.9% sodium chloride injection. The volume ratio of 1,2-propanediol and dimethyl sulfoxide in the injection is 14-20% and 0.25-1% respectively; and the mass / volume ratio of sodium bisulfite in the injection is 0.1-0.3%. The compound sodium salicylate composition can significantly reduce amylase, lipase, IL-6 and IL-1beta, and can be used for preparing a medicine for treating pancreatitis.

The method for catalytically preparing D-mannose provided in the application can effectively reduce the amount of by-products glucose and fructose, and greatly improve the conversion rate of D-mannose. Specifically, the engineering bacteria expressing isoamylase gene and the engineering bacteria expressing alpha-glucan phosphorylase gene, glucose phosphomutase gene, bifunctional enzyme glucose phosphate isomerase / mannose 6-phosphate isomerase gene and mannose 6-phosphate phosphatase gene are used, starch or starch derivatives are used as substrates, phosphate buffer solution and Mg 2+ A preliminary catalytic system is constructed, and after the reaction is completed, there are unreacted substrates and maltodextrin in the reaction system, the saccharifying enzyme can hydrolyze the unreacted substrates and maltodextrin into glucose, the polyphosphate glucose kinase can convert all the glucose into phosphorylated glucose with the assistance of sodium hexametaphosphate, and then D-mannose is generated, thereby improving the yield and removing the by-products in the system.

The invention relates to the technical field of deep processing of starch, in particular to a method for preparing dietary fiber, which comprises the following steps: (1) mixing starch with acid, and baking to obtain pyrodextrin; (2) performing enzymolysis modification on the pyrodextrin obtained in the step (1) in the presence of starch branching enzyme, and then performing saccharification treatment in the presence of glucamylase to obtain crude resistant dextrin liquid; and (3) mixing the resistant dextrin crude liquid obtained in the step (2) with inulin. According to the preparation method provided by the invention, the content of resistant components in the resistant dextrin crude liquid and the yield of the resistant dextrin can be improved, the preparation time of the resistant dextrin is greatly shortened, and the cost is saved. The prepared dietary fiber can be widely applied to food preparation, such as preparation of health-care products, special dietary foods, beverages, seasonings, candies or milk powder.

The invention relates to the technical field of food-grade materials, in particular to a food-grade porous material and a preparation method thereof. The preparation method comprises the following steps: gradually carrying out enzymolysis on corn flour by alkaline protease and amylase under the conditions of proper temperature and pH, continuously stirring, adding food-grade vegetable gum, and forming porous glycoprotein under the assistance of ultrasonic waves; the porous glycoprotein is uniform in pore size and is 0.5-3 microns, and the surface of the porous glycoprotein is rich in functional groups, so that the porous glycoprotein has good adsorption performance and has very high adsorbability on hydrophobic compounds such as metal ions, pesticide residues, kitchen oil stains and the like. The preparation process is simple, chemical reagents do not need to be introduced, and safe and non-toxic substances are completely adopted as materials for preparing the porous material, so that the product is food-grade, small in damage to human bodies and the environment and wide in application in the food field.

The invention relates to a tea seed oil emulsion loaded with grape seed polyphenol-phloretin and a preparation method thereof, and the preparation method comprises the following steps: taking a sucrose-xanthan gum aqueous solution as a water phase, adding grape seed polyphenol into the water phase, taking a polyglycerol polyricinoleate-tea seed oil solution as an oil phase, and uniformly mixing to obtain the tea seed oil emulsion loaded with grape seed polyphenol-phloretin. Grape seed polyphenol can be effectively loaded in an oil phase through a high-speed shearing emulsification method. After the grape seed polyphenol emulsion is prepared, phloretin is added, and the grape seed polyphenol-phloretin loaded tea seed oil emulsion is prepared. According to the method designed by the invention, grape seed polyphenol and phloretin can be stably loaded in an oil solution, so that the problem that water-soluble polyphenol and alcohol-soluble polyphenol are difficult to dissolve in grease is effectively solved; meanwhile, the grape seed polyphenol and the phloretin are compounded for use, so that on one hand, the shelf life of the product can be effectively prolonged, and the oxidation resistance is improved; on the other hand, hydrolysis of alpha-amylase and glucolase on starch can be inhibited, digestion and absorption of carbohydrates are interfered, and powerful technical support is provided for development of novel healthy food.

The invention belongs to the technical field of microbial prevention and control of plant diseases and insect pests, and particularly discloses a plant disease biocontrol bacillus cereus strain LCBC246 which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the strain is CGMCC No.33323. The invention further discloses a preparation method of the plant disease biocontrol bacillus cereus strain LCBC246. The LCBC246 strain provided by the invention is a gram-positive bacterium, has a very strong bacteriostatic effect on colletotrichum fucicola, the bacteriostatic rate is 92.1%, the disease control rate on the colletotrichum fucicola is 90.8%, and the LCBC246 strain has a very good inhibitory effect on other important plateau characteristic crop pathogenic bacteria and also has a remarkable crop growth promoting property. The bacterial inhibition rates of fermentation liquor and volatile substances of the strain LCBC246 on colletotrichum gloeosporioides of macadamia nuts are 62.70% and 76.58% respectively; the bacillus amyloliquefaciens can generate sterilization metabolites such as cellulase, amylase and siderophore, and has a very strong broad-spectrum bacteriostatic effect on various plant pathogenic fungi. Therefore, the strain LCBC246 and the metabolite thereof have the application value of developing bacterial biopesticides.

This invention discloses a method for preparing a mixture of mussel oligosaccharides and the application of the product in inflammatory bowel disease. This invention uses mussel polysaccharide MP-1 as a raw material, processed by α-amylase or Bacillus subtilis (… Bacillus subtilis BNCC185269) obtained mussel oligosaccharide mixtures MOS-A and MOS-B composed of different oligosaccharides. Animal experiments have shown that the two oligosaccharide mixtures prepared by this invention can effectively alleviate intestinal inflammation. Therefore, the mussel oligosaccharide mixtures prepared by this invention are natural and safe drugs or foods for improving inflammatory bowel disease. They can effectively improve inflammatory bowel disease, are safe, non-toxic, and have no side effects. The raw materials are abundant, the steps are simple, the cost is low, and the effects are significant.

The invention discloses a full-biodegradable starch foaming formula and a preparation method, and relates to the technical field of foaming materials.The full-biodegradable starch foaming formula is prepared through collaborative design of composite modified starch, enzymolysis fibers, a nano reinforcing agent and a composite foaming agent in combination with a microwave-assisted gradient hot press molding process. And complete biodegradability and function integration of the material in a normal-temperature natural environment are realized. The product has high mechanical strength, antibacterial property, high transparency and low water absorption buffer performance, the cost is greatly reduced compared with that of a traditional PLA material, industrial composting facilities are not needed, the restriction of performance-degradation-cost in the whole process from raw materials to the process is broken through, and industrial replacement of green materials is achieved.

The invention relates to the technical field of biology, in particular to application of lactobacillus rhamnosus FMBL L23004CNN in fermentation of plums, and the preservation number of the lactobacillus rhamnosus FMBL L23004CNN is CCTCC (China Center for Type Culture Collection) NO: M 20231099. In the process of fermenting the prunus armeniaca, the viable count is increased to 5.10 * 10 < 9 > CFU / mL, and the prunus armeniaca has the advantage of stable growth; the total acid content is generally in a rising trend, the pH is generally in a decreasing trend, and the total phenol content is in a trend of first rising and then decreasing; after fermentation is performed for 24 hours, the total phenol content in the apricot and plum juice is increased to 563.436 mg / L and is remarkably increased by 68.45%; aBTS and DPPH free radical scavenging rates of the fermented apricot and plum juice are remarkably improved; the inhibition rate on alpha-amylase and alpha-glucosidase is obviously improved; the method can be widely applied to fermentation of apricot and plum juice and prolongs the storage life.

The invention discloses high-activity hypoglycemic strigose hydrangea juvenile leaf extract microcapsule powder and a preparation method thereof.The microcapsule powder is prepared through the method comprising the following steps that firstly, a high-voltage pulsed electric field (1-3 kV / cm) is adopted to assist alkalescent electrolyzed water to extract strigose hydrangea juvenile leaf, and a strigose hydrangea juvenile leaf extract (STE) is obtained; and mixing the extract with a wall material composed of at least two of maltodextrin, Arabic gum, inulin and arabinoxylan according to a mass ratio of 1: 0.25-1: 1.25, homogenizing, and spray-drying at an air inlet temperature of 140 + / -5 DEG C and an air outlet temperature of 80 + / -5 DEG C. The obtained microcapsule powder contains various high-content active ingredients such as 0.3 wt%-1.0 wt% of trilobatin and 0.3 wt%-1.0 wt% of phlorizin, and the embedding rate is high (for example, the embedding rate of total flavonoids is 67.53%-88.84%). According to the product disclosed by the invention, the bitter taste of the strigose hydrangea juvenile leaf is remarkably improved, the active ingredients can be effectively protected, the high inhibition activity on alpha-glucosidase and alpha-amylase can be maintained through simulated gastrointestinal tract digestion, the blood sugar reducing function is outstanding, and the product is suitable for preparing food, health care products or medicines with the blood sugar reducing function.

The invention belongs to the technical field of noodles, and discloses Chinese herbal medicine composite noodles with effects of reducing hypertension, hyperglycemia and hyperlipidemia and a preparation method of the Chinese herbal medicine composite noodles. The noodles are prepared by taking wheat flour, buckwheat flour, tartary buckwheat flour and oat flour as bases, matching with functional raw materials such as mulberry leaf powder, semen cassiae powder, hawthorn powder, rumex hanus powder, radix puerariae powder, gynostemma pentaphylla powder and ganoderma lucidum spore powder, and adding a plant extracting solution prepared from apocynum venetum, rhizoma polygonati, mulberries, pericarpium citri reticulatae and poria cocos. The noodles are prepared through vacuum low-temperature dough kneading, twin-screw low-temperature extrusion and sectional hot air drying processes. According to the formula, the synergistic effect of multiple herbal components is achieved, and the obtained noodles are high in content of active components such as total flavonoids and polysaccharides and are stable in the cooking process. An in-vitro experiment shows that the noodles have a remarkable inhibition effect on alpha-amylase and pancreatic lipase and have the effects of assisting in reducing blood sugar, blood fat and blood pressure. The product has good taste, and is suitable for industrial production and daily eating and conditioning of people with hypertension, hyperlipidemia and hyperglycemia.

The invention discloses a ganoderma lucidum spore oil component emulsion, a preparation method thereof and application of the ganoderma lucidum spore oil component emulsion in anti-tumor and anti-oxidation products, and belongs to the field of medicines.The preparation method comprises the steps that 1, ganoderma lucidum spores are softened, emulsified and subjected to wall breaking, and emulsion and spore wall shells are obtained; (2) concentrating the emulsion to obtain a concentrated solution A; (3) performing primary enzymolysis on the spore wall shells by using a compound enzyme A, adding a compound enzyme B for secondary enzymolysis, drying filter residues, and extracting to obtain ganoderma lucidum spore oil; (4) crushing the ganoderma lucidum sporocarp, adding a solvent for soaking and extracting, and concentrating the filtrate to obtain a concentrated solution B; (5) mixing the concentrated solution A, the ganoderma lucidum spore oil and the concentrated solution B to obtain a ganoderma lucidum spore oil component emulsion; the compound enzyme A comprises cellulase and chitinase, and the compound enzyme B comprises papain, bromelain and alpha-amylase. The ganoderma lucidum spore oil component emulsion is prepared by the preparation method. Compared with the prior art, the ganoderma lucidum spore oil component emulsion disclosed by the invention has better anti-tumor and anti-oxidation effects.

The invention relates to the technical field of preparation of sea-buckthorn peel residue degraded polysaccharide, in particular to sea-buckthorn peel residue degraded polysaccharide as well as a preparation method and application thereof. The sea buckthorn peel residue degraded polysaccharide comprises the following monosaccharides: mannose, glucosamine hydrochloride, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose. The method comprises the following steps: extracting and collecting hippophae rhamnoides fruit peel residue crude polysaccharide by taking hippophae rhamnoides fruit peel residue as a raw material through an ultrasonic extraction method, and then degrading the hippophae rhamnoides fruit peel residue crude polysaccharide by adopting a free radical degradation method based on an ascorbic acid-hydrogen peroxide system to obtain the degraded hippophae The prepared sea-buckthorn pericarp residue degraded polysaccharide has the effects of scavenging free radicals, inhibiting the activity of alpha-amylase and inhibiting the activity of alpha-glucosidase, and all the effects after degradation are remarkably improved.

A method is described for increasing the amount of fermentable sugars in a fermentation substrate by treating with a combination of an enzyme having transglucosidase activity and an enzyme having glucoamylase activity to hydrolyze oligosaccharides and / or polysaccharides that are typically unhydrolyzable by glucoamylases alone during fermentation. The method is most effective for fermentation substrates containing small amounts of maltose and maltotriose.

The invention discloses a preparation method of a mussel oligosaccharide mixture and application of a product of the mussel oligosaccharide mixture in inflammatory bowel diseases. Mussel polysaccharide MP-1 is used as a raw material, a mussel oligosaccharide mixture MOS-A and a mussel oligosaccharide mixture MOS-B composed of different oligosaccharides are obtained through alpha-amylase or bacillus subtilis BNCC185269, and animal experiments prove that the prepared mixture of the two oligosaccharides can effectively relieve intestinal inflammation; therefore, the mussel oligosaccharide mixture prepared by the invention is a natural and safe medicine or food for improving the inflammatory bowel disease, can effectively improve and verify the inflammatory bowel disease, and is safe, non-toxic, free of side effects, rich in raw materials, simple in steps, low in cost and remarkable in effect.

The invention discloses a method for constructing a high-yield epsilon-polylysine engineering strain streptomyces diastatochromogenes asnO-argD through overexpression of an asparagine synthetase gene asnO and an N-succinyl diaminopimelate aminotransferase gene argD, and the high-yield engineering strain is applied to fermentation production of epsilon-polylysine. The invention further discloses a preparation method of the high-yield epsilon-polylysine engineering strain and a preparation method of the high-yield epsilon-polylysine engineering strain. The yield of the epsilon-poly-lysine can reach 55.51 g / L through fed-batch fermentation of a fermentation tank of the high-yield strain S. distaatom romogenes asnO-argD 5L, and the yield of the epsilon-poly-lysine is increased by 96.42% compared with the yield of a chassis strain S. distaatom romogenes 6 #-7; compared with a chassis strain S. diastatochromogenes 6 #-7, the production intensity of the strain disclosed by the invention is improved by 66.40%; the epsilon-polylysine / glucose consumption conversion rate (g / g) is improved by 15.76% compared with that of the chassis strain, and the strain has high industrial application value.

Previously, methods have been described for increasing the amount of fermentable sugars in a fermentation substrate by treating it with a combination of an enzyme having transglucosidase activity and an enzyme having glucoamylase activity to hydrolyze oligosaccharides and / or polysaccharides that are not hydrolyzed by glucoamylase alone during fermentation. This method is most effective using a fermentation substrate containing small amounts of maltose and maltotriose.

The invention relates to the field of starch type textile impregnating compounds, and discloses a film-forming agent as well as a preparation method and application thereof. The method comprises the following steps: performing acidolysis and gelatinization on starch slurry to obtain starch paste, mixing the starch paste with pullulanase and isoamylase, and performing enzymolysis. By adopting the method provided by the invention, the film-forming agent suitable for application can be prepared by utilizing the common starch which is easy to obtain, and the produced product is relatively stable in performance, is more friendly to downstream application and has a higher practical production value.