Tobacco biological quality-enhancer, preparation method and application thereof
A biological and tobacco technology, applied in the fields of tobacco, application, and treatment of tobacco, can solve the problems of high irritation, masking of smoke aroma, rough smoke, etc., and achieve the effect of improving aroma and taste and improving quality
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[0084] (1) Preparation of bacterial agent:
[0085] Include the following steps:
[0086] ① Strains: ZXB 1 , Bacillus megaterium (Bacillus megaterium), China General Microorganism Culture Collection Management Center CGMCC, preservation number 1.941; ZXB 2 , Bacillus subtilis subsp.subtilis, China General Microorganism Culture Collection Center CGMCC, preservation number 1.354;
[0087] ② Slant culture: inoculate a ring of bacterial lawn on a fresh slope, and cultivate at 30°C for 2 days;
[0088] ③Expanded cultivation of strain bottle: The formula of culture medium is 70% bran, 28.5% bean cake powder, 1.5% urea, material: water=1:1.0, pH is 9.0, weigh the raw materials according to the formula and mix well, then add appropriate amount Water (material: water = 1: 1.0). Continue to mix until it forms a ball when held in the hand and disperses when touched, then adjust the pH value to 9.0 with 2% NaOH solution, and put them in the strain bottle respectively, filling to The s...
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