Zymolysis method for producing lactic acid by bacillus subtilis to saccharify garbage from restaurant and cookroom

A technology of food waste and Bacillus subtilis, which is applied in the field of food waste fermentation to produce lactic acid, can solve the problem of high cost of lactic acid

Inactive Publication Date: 2007-07-25
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of high cost of producing lactic acid by adding amylase or cellulase to food waste, and provide a method for producing lactic acid by saccharifying food waste and fermenting with Bacillus subtilis

Method used

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  • Zymolysis method for producing lactic acid by bacillus subtilis to saccharify garbage from restaurant and cookroom
  • Zymolysis method for producing lactic acid by bacillus subtilis to saccharify garbage from restaurant and cookroom

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specific Embodiment approach 1

[0006] Specific Embodiment 1: In this embodiment, Bacillus subtilis saccharifies and ferments food waste to produce lactic acid through the following steps: (1) filtering and pulverizing the food waste; (2) adding subtilis accounting for 0.8% to 1.2% of the food waste mass (3) adding Bacillus subtilis to the food waste and saccharifying it for 25-35 hours at 35-45°C; (4) adding water 0.5-1.5 times the mass of solid food waste; (5) adding a solid-liquid mixed meal Lactic acid bacteria with 3% to 5% of the quality of kitchen waste, and adding calcium carbonate to maintain the pH value of the saccharified food waste at 6.2 to 6.4, and fermenting the saccharified food waste at 40 to 50°C for 26 to 30 hours; ( Six) solid-liquid separation, and then purifying the lactic acid in the filtered fermentation broth to obtain lactic acid.

[0007] Bacillus subtilis can directly saccharify and ferment food waste or other organic waste.

specific Embodiment approach 2

[0008] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: step (4) adding water with 0.7 to 1.3 times the mass of solid kitchen waste. Other steps are the same as those in Embodiment 1.

specific Embodiment approach 3

[0009] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the water that adds in the step (four) is tap water, kitchen waste filtrate or dining room, canteen waste water. Other steps are the same as those in Embodiment 1 or 2.

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Abstract

This invention relates to a method for preparing lactic acid from food garbage by saccharification with Bacillus subtilus. The method solves the problem of high cost by adding amylase or cellulose into food garbage to prepare lactic acid. The method comprises: (1) filtering food garbage, drying, and pulverizing; (2) adding Bacillus subtilus; (3) saccharifying; (4) adding water; (5) adding lactic acid bacteria, adding calcium carbonate, and fermenting; (6) filtering liquid and solid, and purifying lactic acid in the filtrate to obtain the final product. The method does not need high-temperature sterilization. The yield of lactic acid is 50-55.7g lactic acid/L solid-liquid-mixed food garbage, and the content of lactic acid content in organic acids in the fermentation liquid is 94-97 wt.%. Besides, the cost is saved by 20-26% compared with that of adding diastase or cellulase.

Description

technical field [0001] The invention relates to a method for producing lactic acid by fermentation of kitchen waste. Background technique [0002] Food waste is a general term for food waste discarded by households and catering industries, and it is a solid waste formed in the process of people's daily consumption. Kitchen waste is biomass waste with high organic content, mainly including starch, cellulose, protein, etc. It can be reused as biomass energy, and it occupies a large proportion of municipal solid waste. Using kitchen waste to produce lactic acid, and then purifying and synthesizing polylactic acid is one of the effective ways to realize its resource utilization and reduction. [0003] Lactic acid bacteria can only produce lactic acid with simple sugars such as glucose as a substrate. If lactic acid bacteria are used to directly ferment kitchen waste, the saccharification rate of complex organic matter (such as starch, cellulose, etc.) in the garbage is low, so ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12R1/125
Inventor 汪群慧陈丽玮王孝强尹玮
Owner HARBIN INST OF TECH
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