466 results about "Amyris" patented technology

A method for the production of ethanol and a modified animal feed is provided. The method replaces the starch in known corn-based animal feed with biomass fiber treated to make it more digestible by animals. The process includes wherein the pericarp and germ are removed from the corn kernel and processed for by-products. The starch and protein are also removed and separated. The starch is then fermented and distilled to ethanol and stillage. The bioavailable modified animal feed comprises the pericarp and germ removed from corn kernels and optionally by-products of the pericarp and germ processing, and lignocellulosic materials. The modified animal feed may optionally include energy materials such as animal and vegetable fats, vegetable soapstocks, or glycerin, and combinations thereof.

The invention relates to a vinegar melody, biological vinegar and the preparing method, which dissolve the problem that glacial acetic acid or the like in the edible vinegar has side effect for the human. The vinegar melody is made by Avena sativa, wheat, black rice, peart barley, soya bean, black soya bean, green bean, red bean, broad bean, pea, tea, traditional Chinese medicine herbal pieces prepared for decoction and vinegar melody bacterial. The preparing method of the vinegar melody comprises the following steps: 1, boiling after disintegrating high starch raw material and infiltrating with the water; 2, adding the other raw material, water and vinegar melody bacterial and fermenting. The biological vinegar is made by vinegar melody, Daqu, Shenqu, bran melody, wine yeast, aspergillus oryzae, Chinese sorghum, corn, millet, glutinous millet, peart barley, glutinous rice, black rice, citrate bacterial, yeast, acetic acid bacteria and fruit normal juice. The preparing method of the biological vinegar comprises the following steps: 1, boiling after disintegrating high starch raw material and infiltrating with the water; 2, adding the other raw material and saccharifying and acetous fermenting zymogeneous bacteria; 3, adding the salt and afterripening; 4, drenching the vinegar; ageing; sterilizing and filtering. The local flavor of the biological vinegar comes from the raw material and the metabolite of the microorganism completely without the glacial acetic acid or the like, and the biological vinegar doesn't have the side effect for the human and has the delicious taste and more than 8. 13g / 100ml high vinegar yield.

Sweeteners on the basis of a simultaneously transglucosylated sweet glycoside mixture of Stevia rebaudiana Bertoni are prepared. The transglucosylation was developed in the presence of starch under the action of cyclodextrin glucanotransferase. The remaining maltodextrins are transformed to the fructose-terminated oligosaccharides by the addition of sucrose. The sweeteners are purified to not less than 98% content of sweet glycosides and derivatives. The preparations are almost non-caloric, non-cariogenic, non-bitter, non-lingering sweeteners, which may be advantageously applied in foods, beverages, cosmetics and milk products.

Cornstarch characteristics can be changed by expressing a non-corn plant starch branching enzyme in a corn plant. In a preferred embodiment, transgenic corn plants containing the barley starch branching enzyme IIa transgene was generated. Some transgenic corn plants produced seeds containing cornstarch with lowered gelatinization temperature and retrogradation rate while others produced seeds containing cornstarch with higher retrogradation rate when compared to non-transgenic corn plants.

In the invention, a bacillus psychrosaccharolyticus obtained by screening is used for preparing an efficient complex microorganism seaweed fertilizer. The bacillus strain in the invention is named as bacillus psychrosaccharolyticus and preserved in the Common Microorganism Center under China Microorganism Strain Preservation and Management Committee with a preservation number of CGMCC No.4519. The bacillus psychrosaccharolyticus not only has the capacity of carrying out forced degradation on cellulose and starch but also has a wide antimicrobial spectrum; the bacillus psychrosaccharolyticus strain can be compounded with the traditional common strain for bacterial manure; then mixed zymocyte liquids, plant growth factors, immunoregulation factors, active extractives from seaweed, amino acids, organic matters and trace elements are prepared into an efficient complex mircoorganism seaweed fertilizer with an active viable count of 0.2 billion / g to 1 billion / g and an organic matter content of 20-40% according to a certain proportion. The fertilizer has remarkable capacity in combating plant diseases and insect pests and promoting growth. With the fertilizer, the yield of crops can be increased by more than 30%.

The invention discloses a bone soup seasoning, which is characterized by comprising the following raw materials in parts by weight: 45-50 parts of fresh bones, 6-10 parts of pigskins, a right amount of white sugar, a right amount of salt, a right amount of powdered monosodium glutamate, 2-4 parts of disodium 5'-ribonucleotide, 2-3 parts of mashed garlic, 4-5 parts of pitaya flowers, 3-4 parts of peach leaves, 2-3 parts of ginger, 10-15 parts of starches, 1-2 parts of Rhodiola rosea, 3-5 parts of agaricus blazei murill, 1-2 parts of bengal waterdropwort herbs, 2-3 parts of hericium, 3-5 parts of rhizoma ligustici wallichii and 0.08-0.12 part of xanthan gum. Through adding traditional Chinese medicinal materials such as pitaya flowers, peach leaves and rhodiola rosea, the seasoning disclosed by the invention not only has the characteristics that the seasoning is fresh, fragrant, delicious and rich in tastes, but also enhances the health care value, and further enhances the health-care benefits of the product.

Disclosed is a processing method for avena sativa rice, comprising selecting stock husk avena sativa seeds, grading, decorticating, enzymes inactivating, screening, drying, and packaging. A decortication machine is used to decorticating avena sativa seeds, without seeds breakage. The decortication ratio maintains at 6% by adjusting space according to seed size. Enzymes inactivation is carried out by treating with steam, at 100 DEG C, for 20min, or treating with high pressure water steam for 10min; by using a hyperthermia instant enzyme inactivation method, lypase, proteinase and amylase can be inactivated in 20s. After that, screeing, drying and packaging are carried out. The method is provided with simplicity, avena sativa rice produced thereby has a long preservation time, and the precessing time is short.

The present invention provides a method to make a blue colorant as a liquid and as a powder, wherein the blue colorant is derived from unprocessed raw juice obtained from Genipa americana fruit pulp, and wherein said raw juice is mixed with glycine (liquid) or with glycine plus starch (powder). Except for an additional step of warming up the juice-glycine mix, and in the case of the powder further dehydration of the juice-glycine-starch remix, no further steps are required to make a temperature and PH stable blue colorant which may be applied in textile, pharmaceutical, food, cosmetics, and other industries.

The invention belongs to a preparation method of low-molecule brown seaweed glucan. Alkali carbonate solution is used for breaking up seaweed tissue and cells, acid is used for adjusting a pH value to be proper, alginic acid, brown seaweed polysaccharide and brown seaweed starch are extracted from the seaweed tissue and the cells, and the starch is degraded with hydroxide to turn into the brown seaweed glucan with the average molecular weight being 800-1000 Dalton. As reported, the brown seaweed glucan with the low molecular weight can enhance the immunity of a human body and the stress resistance of plants.

The invention provides a delicious edible fungus product, which is produced by pulverizing, mixing and granulating the following raw materials, in mass ratio, edible fungus aqueous extract drying powder 5-25 parts, monosodium glutamate 10-25 parts, kitchen salt 10-40 parts, I+G nucleotide 0.5-5 parts, maltodextrin or starch 8-40 parts and white sugar 10-20 parts. The resourceful edible fungus is selected as the raw material of the delicious product provided by the invention, and the product has the advantages of simple preparation process, easy operation, low cost, delicious taste, high nutrition value and some health-care effects.

The present invention relates to pharmaceutical compositions comprising ester(s) of capsaicin and at least one other agent selected from salicylates, menthol, boswellic acids, DMSO, methyl sulfonylmethane, NSAIDs, corticosteroids, emu oil, opioid agonists and antagonists, NMDA antagonists, tramadol, hyaluronic acid, α2δ ligands, santalol, santalyl acetate, amyris alcohol, amyris acetate, aloe vera gel and aloe vera juice, for improved therapeutic properties. Further, the present invention relates to pharmaceutical compositions comprising high concentrations of ester(s) of capsaicin. Further, the present invention relates to a method of relieving pain due to various diseases in subjects by administering the pharmaceutical compositions of the invention. Further, the present invention relates to methods of relieving fever blisters due to cold sores in subjects by administering the pharmaceutical compositions comprising an ester of capsaicin and one other agent selected from santalol, santalyl acetate, amyris alcohol and amyris acetate.

The invention discloses a shampoo containing natural saponin and natural thickener, which contains natural saponin, natural thickener and water, wherein the natural saponin at least comprises one or mixture of Chinese soapberry saponin tea saponin; and the natural thickener at least comprises one or more of any of natural plant starch, xanthan gum, arabic gum, guar gum, carrageenin and seaweed gel. The natural saponin and natural thickener are matched, so that the shampoo has the viscosity suitable for daily life and can mildly clean the hair without irritating or damaging the scalp. The natural raw materials are adopted to avoid the risk of introducing harmful chemical synthetic substances into the formula.

The invention discloses a bacillus amyloliquefaciens D2WM as well as a preparation method and application of bacillus amyloliquefaciens D2WM. The bacillus amyloliquefaciens D2WM comprises the steps of A, bacillus separation and purification, namely collecting after inoculating a slope; B, preliminary screening of bacillus, namely sequentially selecting strains having antagonistic effects on Erwinia and camellia oleifera diseases by using a point connection method and a plate confrontation method; and C, secondary screening of bacillus, namely culturing the preliminarily-screened strains with the antagonistic effects in an LB liquid culture medium by using a cup-plate method, and screening the strains simultaneously having the antagonistic effects on main diseases of Erwinia and camellia oleifera through an antagonistic bacterium fermentation liquid and sterile filtrate. When being applied to preparation of drugs for treating or preventing soft rot of vegetables and camellia oleifera diseases, the bacillus amyloliquefaciens has a broad-spectrum antagonistic effect, high in growth speed, capable of efficiently antagonizing anthracnose of camellia oleifera, leaf spot diseases of camellia oleifera, black spot of camellia oleifera, canker of camellia oleifera and various diseases and remarkable in antibacterial effect, the culture time for general bacteria and fungi is greatly shortened, and the production cost is reduced.

The present invention relates to bacillus amyloliquefaciens and a preparation method of a microbial inoculum of the bacillus amyloliquefaciens, and belongs to the field of bio-pesticides and plant protection. The bacillus amyloliquefaciens HN-11 is isolated from azadirachta indica tree rhizosphere soil, azadirachta indica residue is used as a medium raw material and a microbial inoculum adsorption carrier for secondary solid state fermentation composting to prepare the microbial inoculums, the microbial inoculums comprises an arbitrary content of the bacillus amyloliquefaciens, 0 to 30% of the azadirachta indica residue, 4 to 12% of the soybean powder, 5 to 25% of wheat bran, 30-50% of peat, 1-5% of KCl, 0.5 to 2% of urea and 1 to 8% of a surfactant. Tests show that the microbial inoculum can overcome the problem of seedling injury caused by direct use of the azadirachta indica residue, can expand the control spectrum, has obvious integral control effect on banana wilt and Radopholus similes Thorne, can also promote plant growth, and the field control effect on the banana wilt can reach 96.2%. The microbial inoculum has great significance to the promotion of integral pest control and agricultural and sideline product recycling, and has broad application prospects in the production practice.

The invention provides a method for extracting cornel polysaccharide through enzymolysis. The method comprises the following steps that cornel dry flesh is crushed to make cornel dry powder; distilled water is added with the mass being 15 to 60 times than that of the cornel dry powder, and an enzyme are added so as to carry out enzymolysis at a temperature of between 35 and 60 DEG C and a pH value of between 3.5 and 7.0; after the enzymolysis is finished, enzyme inactivation is carried out; an extraction solution is separated and purified so as to obtain a cornel polysaccharide extract; the enzyme is a mixture comprising one sort or more than two sorts of (1) cellulase, (2) papain, (3) pectinase and (4) amylolytic enzyme; and the dose by mass of the enzymes accounts for 0.1 to 24 percent of the mass of the cornel dry powder. The method has the advantages that compared with the prior hot water extraction method, the method can increase the extraction rate of cornel polysaccharide by 10 to 50 percent and the content of cornel polysaccharide by 15 to 80 percent; and due to preventing the influence of the prior hot water extraction, acid extraction or alkaline extraction on the structure of polysaccharide, and adopting enzymolysis treatment to extract cornel polysaccharide, the method has the advantages of moderate conditions, easy removal of impurities, furthest maintained activity of polysaccharide, and the like.

The invention provides an application of application of plant alpha-diastase in pollen abortion, and belongs to the technical field of genetic engineering. Through introducing polynucleotide of the plant alpha-diastase, recombinant vector and recombinant vector are structured; a pollen specific promoter drives the plant alpha-diastase to degrade starch in advance during the pollen developmental phase; the pollen sprouting energy cannot be provided, and the pollen sprouting is contained, thus abortion is generated; the transgenetic seedling of transgene pollen abortion is obtained. The plant alpha-diastase can be used as the pollen abortion gene and can be originated from genes of rice endogenesis, millet, broomcorn, barley, wheat and other gramineous plants; the plant alpha-diastase is very good for transgenic plant of pollen abortion. The plant alpha-diastase gene has accurate expression level and can control the spreading of transgenosis; the plant alpha-diastase gene also can keep and breed a male sterility line while leave out the artificial castration step during the hybrid seed production process; the application prospect is wide.

The invention discloses tomato sauce-containing quick-eating chicken fillets which comprise the following raw materials in parts by weight: 200-220 parts of chicken breast meat, 130-150 parts of tomato sauce, 30-40 parts of eggs, 100-120 parts of starch, 20-30 parts of almonds, 20-30 parts of coix seeds, 10-20 parts of yellow beans, 5-6 parts of colla corii asini, 4-5 parts of aloes, 4-5 parts of wheatgrass, 5-6 parts of cinnamon, 5-6 parts of mulberries, 5-6 parts of zornia gibbosa, 3-4 parts of gardenia, 3-4 parts of lavenders, 25-35 parts of salt, 20-30 parts of chicken essence, 230-260 parts of olive oil, 100-120 parts of milk and a proper amount of water. The tomato sauce-containing quick-eating chicken fillets provided by the invention are delicious, sour and sweet; food materials such as the eggs and the almonds with rich nutrients are added, so that nutrients necessary for a human body can be supplemented; extracts of Chinese herbal medicines such as the colla corii asini and the aloes with effects of nourishing blood and removing toxins are also specially added, so that the tomato sauce-containing quick-eating chicken fillets have very good effects of maintaining beauty and removing toxins and are a healthy and delicious meat food suitable for broad masses of people with the love of beauty.

The invention belongs to the field of Chinese medicinal herb development. The method aims at improving the utilization ratio of natural plant Chinese medicinal herbs, utilizing on the basis of the traditional method and integrally extracting the active components contained in elsholtzia haichowensis sun. The method comprises the following steps: crushing a commercially available elsholtzia haichowensis sun medicinal material dried in the shade into powder capable of being screened through sieve with the mesh size of 40-80 meshes, soaking, and extracting volatile oil through a steam distillation method; filtering a water solution, retaining filter liquor and filter residues, adding a certain amount of starch into the filter liquor, and carrying out spray drying to obtain elsholtzia haichowensis sun polysaccharide; airing the filter residues, adding ethanol for organic reflux, and extracting total flavone. The method disclosed by the invention can be used for realizing the integrated utilization of elsholtzia haichowensis sun resources and has the advantages of simple process, less production process and low cost.

The present invention relates to a novel microorganism Bacillus amyloliquefaciens KTGB0202 and a method for controlling plant pathogens using the same. More particularly, the present invention relates to a novel microorganism Bacillus amyloliquefaciens KTGB0202 (accession number: KCTC 10564BP) bacterium which has an excellent control effect against crop powdery mildew and a broad spectrum of antifungal activity against plant pathogenic fungi and inhibits tobacco mosaic virus infection, as well as an eco-friendly bacterial culture broth for controlling powdery mildew, which contains the same. Also, the present invention relates to antifungal substance KTGB0202 AFO1 obtained by extraction and purification from the Bacillus amyloliquefaciens KTGB0202 bacteria. The inventive Bacillus amyloliquefaciens KTGB0202 bacteria have a broad spectrum of antifungal activity, and are excellent in activity continuance and used for controlling various plant pathogens, including powdery mildew.

The invention provides organic basic fertilizer with traditional Chinese medicines for winter wheat. The organic basic fertilizer is prepared from the following raw materials in parts by weight: 80-100 parts of alga, 50-80 parts of soybean cake meal, 50-80 parts of plant ash, 15-20 parts of dalmatian chrysanthemums, 15-20 parts of kusnezoff monkshood roots, 15-20 parts of chili leaves, 5-10 parts of mint leaves, 15-20 parts of plant sterol, 20-30 parts of fulvic acids, 20-30 parts of ascorbic acids, 50-80 parts of potassium dihydrogen phosphate, 50-80 parts of coated urea , 50-80 parts of sodium borate, 50-80 parts of calcium superphosphate, and 20-50 parts of starch. The preparation method of the organic basic fertilizer with the traditional Chinese medicines for the winter wheat, disclosed by the invention, is simple. The traditional Chinese medicine raw materials are added, so that the organic basic fertilizer has the functions of killing bacteria and pests, preventing various physiological diseases which are easily produced in the winter wheat and enhancing the resistance of crops, multiple functions of fertilizer effects, killing the bacteria, killing the pests and rooting are integrated, the fertilizer effects are adequate, the utilization rate of the fertilizer is high, and the production increase effect is obvious.

The invention provides health-care vinegar, a vinegar drink preparation method and anti-alcohol liver-protection application thereof. The method comprises the following steps: (1) grinding rice and kudzuvine root into thick liquid with water, regulating the concentration of starch to be over 10 percent with sterile water or traditional Chinese medicinal extract, and heating the mixture at the temperature of between 125 and 130 DEG C; (2) adding rhizopus, activated dried yeast, aroma-producing yeast and lactic acid bacteria into the sterilized mixture to ferment distilled wort, and stopping alcoholic fermentation when the alcohol content is between 10 and 15 percent; (3) regulating the alcohol concentration to be between 6 and 8 percent with the sterile water or the traditional Chinese medicinal extract, adding acetic acid bacteria solution which is 10 percent of fermentation broth by volume into the mixture at the temperature of between 26 and 34 percent, and stopping fermentation until the acidity of the fermentation broth is between 4 and 6 percent; and (4) ageing the fermented acid solution for over 15 days at the temperature of between 12 and 20 DEG C, and filtering the solution to obtain the health-care vinegar. The invention extracts effective ingredients of traditional Chinese medicament in a flash way to perform alcohol fermentation and acetic fermentation with the kudzuvine root and the rice, not only can effectively maintain the effective ingredients of the traditional Chinese medicament, but also can better play the functions of relieving alcohol, dispelling the effect of alcohol and protecting liver.

The invention belongs to the technical field of agricultural microbiology, and relates to the fields of biochemistry and molecular biology simultaneously. Through separation, a bacillus amyloliquefaciens strain CH1 for producing a growth-promoting fungus apoptosis lipopeptide is obtained, which is preserved in the China Center for Type Culture Collection (CCTCC) with the preserving number of CCTCC: M208127. A bioactive substance is obtained from a culture of the bacillus amyloliquefaciens strain CH1 and is characterized as the growth-promoting fungus apoptosis lipopeptide. The molecular weight of the lipopeptide is 1,065 or a homologue thereof. The lipopeptide has an infrared spectrum as shown in Figure 6 in the instruction and consists of 9 carbon alkyls and 8 amino acids, wherein the seventh alkyl carbon and the last amino acid form a cyclic peptide through an oxygen atom. The bacillus amyloliquefaciens strain and the lipopeptide prepared from the same are tested on the biological application prospect in apoptosis.

The invention discloses a preparation method of highland barley broad bean fine dried noodles. The method comprises the following steps of: (1) mixing of raw materials; (2) dough kneading and curing; (3) tabletting; (4) slitting; (5) drying; (6) cutting off; and (8) packaging. The fine dried noodles are manufactured by using highland barleys and broad beans as raw materials, and effective components in the highland barleys and broad beans can be fully utilized; because the content of straight-chain starch in the starch of broad bean powder is high, the characteristics of low content of the straight-chain starch in the highland barleys and high content of branch-chain starch can be supplemented, and the stickiness of the dough is reduced; the proteins in the broad beans can be used for compensating the deficiency of the proteins and amino acid compositions in the highland barleys and intensifying the nutrition of the highland barley flour; and the broad bean powder is added into the highland barley flour to replace the addition of other external source starch and proteins, so that the process is simplified, and the cost is reduced.

The invention relates to a culture medium for tall gastrodia tuber germination strains. The culture medium is characterized by being prepared from the following raw materials in percentage by weight: 60 to 80 percent of dried aucuba japonica fruits, 10 to 20 percent of wheat bran, 5 to 20 percent of saw dust, 0.8 to 3 percent of sugar and 0.8 to 3 percent of starch. The aucuba japonica fruits have abundant nutrition, are large and plump and store more nutriments, and the effective support time can reach over 130 days, so tall gastrodia tuber protocorm on granular strains has vigorous vitalityand is thick and strong and strives for more time for halimasch inoculation of a large number of tall gastrodia tuber seeds; therefore, the yield of the generative propagation of tall gastrodia tuberis improved substantially. In addition, the aucuba japonica fruits are consistent in size basically and are elastic, have more porous structures and multilayer tissues and are favorable for the ingestion of ingredients and nutrition. The inoculation rate of the granular germination strains of the aucuba japonica fruits can reach 12 to 13.5 percent.

A cool and refreshing tea beverage for reducing internal heat is prepared from the following raw materials, by weight, 40-50 parts of green tea leaf, 4-6 parts of mint leaf, 5-7 parts of isatis root, 1-2 parts of tomato seed oil, 12-15 parts of balsam pear, 10-14 parts of pumpkin seed, 8-10 parts of oat, 8-10 parts of camomile, 5-6 parts of honeysuckle flower, 4-5 parts of salt, 2-4 parts of ephedra stem, 13-15 parts of mung bean, 2-3 parts of beer, 18-22 parts of rock sugar, 2-4 parts of an auxiliary agent and 800-1000 parts of water. The tea beverage provided by the invention is cool and refreshing and has a characteristic of reducing internal heat. The tea beverage contains cool traditional Chinese medicines and food such as chamomile, mint leaf, ephedra stem, isatis root, honeysuckle flower and the like, wherein camomile and isatis root even can be independently used as main materials of herbal tea. According to the invention, pumpkin seed, mung bean and oat are fried to burnt, starch-based food materials give out special burnt fragrance at high temperature, and the effect is better by soaking; honeysuckle flower, isatis root, mint leaf, ephedra stem, camomile and the like are firstly boiled with big fire and then concentrated with soft fire; through fine filtration, residues do not enter a finished production so as to guarantee quality and shelf-life of the finished product; and especially, the addition of balsam pear is the icing on the cake, making the taste much better.

The invention discloses a method for brewing multi-distiller yeast multi-cereal three-fragrance type white wine, belongs to the technical field of white wine brewing, and mainly provides a method for brewing white wine with faint fragrance, thick fragrance and maotai fragrance by using a hybrid process. The method is mainly characterized by comprising the following steps: adding high-temperature yeast for making hard liquor in a faint maotai fragrance brewing process, so as to generate faint maotai distiller yeast containing 40-45% of starch, adding the faint maotai distiller yeast in a thick maotai fragrance brewing process, feeding middle-layer distiller yeast, fermenting the thick fragrance by using middle-high temperature yeast for making hard liquor, feeding lower-layer distiller yeast, performing double-bottom fermentation by using high temperature yeast for making hard liquor, and performing hybrid distillation, separated aging and refined blending in a hybrid brewing process. The method integrates the advantages of processes of three basic fragrances of whit wine in China and has the process characteristics of coexistence and harmony of three fragrances, and the brewed three-fragrance type white wine is colorless, transparent, fragrant, graceful, mellow, soft, full, sweet, clear, harmonious in taste and fragrance, and has the unique characteristics that the thick fragrance, the faint fragrance and the maotai fragrance are integrated. The method is mainly applied to brewing of the multi-distiller yeast multi-cereal three-fragrance type white wine with the faint fragrance, the thick fragrance and the maotai fragrance.

The invention discloses an extraction and purification method for purple sweet potato anthocyanin. According to the extraction and purification method, an alpha-amylase method is adopted to carry out enzymolysis processing on purple sweet potato entire powder so as to achieve the purpose of maximally extracting purple sweet potato anthocyanin, and meanwhile, AB-8 adsorbent resin is adopted to carry out purification, so that the yield and the quality of the purple sweet potato anthocyanin are greatly improved, the extraction rate reaches up to 72.223 mg / g, and the color value is that E<1%>[1cm]m(530nm)=92. The extraction and purification method comprises the steps of: adding the alpha-amylase with the enzyme activity of 200 U in each mL of purple sweet potato emulsion to carry out the enzymolysis processing, carrying out adsorption and purification by utilizing AB-8 macroporous adsorption resin after centrifuging, and carrying out freeze drying to obtain purple sweet potato anthocyanin samples after concentrating. The extraction and purification method is applied to the field of pigment extraction and particularly applied to extraction of the purple sweet potato anthocyanin, the yield and the quality of the purple sweet potato anthocyanin are improved, and a novel concept is provided for the industrial production of the purple sweet potato anthocyanin is provided; and the extraction and purification method can be widely applied to deep processing of the purple sweet potato and other pigment extraction fields.

The invention discloses bacillus amyloliquefaciens DPPG-74. The bacillus amyloliquefaciens DPPG-74 has an accession number of CGMCC No.9396. The bacillus amyloliquefaciens DPPG-74 is screened from rhizosphere soil in a processing tomato severe root rot zone and roots suffering from severe root rot, is gram-positive bacterium, has wide spectrum bacteriostatic activity and very strong stress resistance, can multiply and colonize on surfaces of fungal pathogenic mycelium, effectively inhibits pathogen growth, controls diseases, has high toxicity to pathogens and can effectively prevent and control Xinjiang processing tomato root rot in a seedling stage. Plants treated by the fermentation bacteria liquid has height, fresh weight and dry weight respectively improved by 50.39%, 91.98% and 40.41%, prevention and control rate is 67.74% and nutrient biomass of the plants is effectively improved. The bacillus amyloliquefaciens DPPG-74 allows simple culture conditions, can be preserved easily, is environmentally friendly and has good development and popularization application potential.