Preparation method of low-molecule brown seaweed glucan

A technology of glucan and fucoidan, which is applied in the field of preparation of low molecular weight fucoidan, and can solve the problems of no large-scale production and application of fucoidan

Active Publication Date: 2011-08-17
北京雷力联合海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

my country is a large brown algae breeding country in the world, with an estimated annual output of more than 80,000 tons. At present, the production scale of alginate in my country is very large, and it has been widely u

Method used

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Examples

Experimental program
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Effect test

example 1

[0014] (1) Preparation of algal starch

[0015] Take 100g and crush it to 1~5cm 2 Put the kelp into 2000mL solution containing 30g of sodium carbonate, heat and stir at 80°C for 0.5 hours to disintegrate the tissues and cells of the kelp, slowly add hydrochloric acid to neutralize to pH 4, and then extract for 2 hours; centrifuge or filter, Discard the centrifuged residue or filter residue (can be used to produce seaweed fertilizer), the supernatant liquid passes through ultrafiltration membrane or hollow fiber ultrafiltration column with a molecular weight cut-off of 3000 Daltons, concentrates to 220mL, and adds 100mL absolute ethanol to precipitate alginic acid Then add 250mL of absolute ethanol to the 300mL centrifugation liquid to precipitate the alginate and fucoidan, centrifuge the precipitate, and dehydrate and dry it to obtain the alginate and fucoidan; then continue to add absolute ethanol to the 530mL centrifugation liquid, Make the ethanol concentration reach 85%, ...

example 2

[0019] (1) Preparation of algal starch

[0020] Take 200g of wakame crushed to 20 mesh, put it into 5000mL solution containing 50g of potassium carbonate, stir and extract at 80°C for 0.5 hours, neutralize with sulfuric acid to pH 2, continue stirring and extracting for 4 hours, centrifuge or filter, discard the centrifuge residue or filter residue (can be used to produce seaweed fertilizer), the supernatant liquid is concentrated to 600mL through an ultrafiltration membrane or a hollow fiber ultrafiltration column with a molecular weight cut-off of 3000 Daltons, and 250mL of absolute ethanol is added to precipitate the alginic acid; Add 620mL of absolute ethanol to 810mL of centrifugation liquid to precipitate algin and fucoidan, centrifuge to separate the precipitate, and dehydrate and dry to obtain algin and fucoidan; then continue to add absolute ethanol to 1400mL of centrifugation liquid to make the ethanol reached 80%, the precipitate was centrifuged, dehydrated and drie...

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Abstract

The invention belongs to a preparation method of low-molecule brown seaweed glucan. Alkali carbonate solution is used for breaking up seaweed tissue and cells, acid is used for adjusting a pH value to be proper, alginic acid, brown seaweed polysaccharide and brown seaweed starch are extracted from the seaweed tissue and the cells, and the starch is degraded with hydroxide to turn into the brown seaweed glucan with the average molecular weight being 800-1000 Dalton. As reported, the brown seaweed glucan with the low molecular weight can enhance the immunity of a human body and the stress resistance of plants.

Description

technical field [0001] A preparation method of seaweed extract. technical background [0002] Seaweed is one of the most abundant resources in the world, especially large brown algae such as kelp and wakame, which have been successfully cultivated artificially, and the output has greatly increased. It is estimated that the output of kelp in the world exceeds 200,000 tons. my country is a large brown algae breeding country in the world, with an estimated annual output of more than 80,000 tons. At present, the production scale of alginate in my country is very large, and it has been widely used in food processing. The production of fucoidan started relatively late, but it has developed rapidly and has been applied For health food or medicine, alginate has no large-scale production and application. According to reports, both kelp and wakame contain fucoidan, the content is about 1%, and it is composed of glucose with β-1, 3 structure, and also contains a small amount of β-1, 6 ...

Claims

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Application Information

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IPC IPC(8): C08B37/02A01P21/00
Inventor 汤洁
Owner 北京雷力联合海洋生物科技有限公司
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