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Method for extracting various active ingredients from dendrobium officinale

A technology of Dendrobium candidum and active ingredients, which is applied in the field of extraction and separation of active ingredients of Chinese herbal medicines, can solve the problems of effective utilization of unfavorable raw materials, low extraction efficiency, long extraction time, etc., so as to improve effective extraction efficiency, comprehensive utilization rate, and high extraction efficiency , the effect of short extraction time

Inactive Publication Date: 2013-05-22
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction techniques of Dendrobium officinale polysaccharides mainly include traditional hot water extraction, ultrasonic-assisted extraction and microwave-assisted extraction. The traditional extraction methods have disadvantages such as long extraction time and low extraction efficiency, which are not conducive to the effective utilization of raw materials.

Method used

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  • Method for extracting various active ingredients from dendrobium officinale

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A method for extracting active ingredient free polysaccharides from Dendrobium officinale, specifically comprising the following steps:

[0055] The first step: material pretreatment:

[0056] Weigh 80g of fresh Dendrobium candidum stored at -20℃, crush it, and add 1200ml of ultrapure water (solid-to-liquid ratio 1:15) for later use.

[0057] The second step: the crushed Dendrobium candidum is subjected to flash extraction:

[0058] According to the conditions of flash extraction time 2min (1min / time) and flash extraction rotation speed 4000rpm, the crude extract of Dendrobium candidum was obtained.

[0059] Step 3: Centrifuge the crude extract:

[0060] Centrifuge the crude extract after flash extraction, using a normal temperature centrifuge with a rotation speed of 5000rpm, and centrifuge for 15min. The water-soluble free polysaccharide exists in the centrifuged supernatant A, and the centrifuged precipitate A is retained. At this time, the polysaccharide exists i...

Embodiment 2

[0068] The difference between this example and Example 1 is that it includes the process of preparing the conjugated polysaccharide, specifically:

[0069] Add pure water (solid-to-liquid mass ratio: 1:3) to the precipitate A obtained by centrifugation in the third step, stir well to make it uniform, add cellulase (dry basis) according to the mass of precipitate A at 0.6%, and add cellulase according to the mass of precipitate A Add papain at 1.5%, adjust to PH = 5, place in a water bath at a temperature of 50°C, stir with a stirrer, and react for 1.5h. After completion, the reacted solution was heated to boiling and kept boiling for 3 minutes to remove excess protein. Then centrifuge at 5000 rpm for 15 minutes, the bound polysaccharide exists in the supernatant B after centrifugation, and the precipitate B after centrifugation is preserved. Take the supernatant B and add ethanol until the volume concentration of ethanol in the final solution reaches 80%, place it for 1.5h, u...

Embodiment 3

[0071] The difference between this embodiment and embodiment 2 is: it also includes the step of extracting alkaloids, specifically:

[0072] Add ammonia water to the precipitate B obtained in the step of Example 2 for alkalization. After alkalization, add ethanol with a purity of 90%, and centrifuge to get the supernatant C. After the supernatant C was concentrated, chloroform was added, and the chloroform volatilized to obtain a solidified alkaloid, and the mass of the alkaloid was weighed to be 0.15 g.

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Abstract

The invention discloses a method for extracting various active ingredients from dendrobium officinale. The method comprises the following steps of: carrying out flash-extracting; centrifuging the flash-extracted crude extracting solution; carrying out alcohol precipitation and tertiary dehydration to the centrifuged liquid supernatant to obtain free polysaccharide; combining the centrifuged precipitates with polysaccharide and alkaloid extract; and extracting the various active ingredients from the dendrobium officinale, so that the comprehensive utilization of the dendrobium officinale resources is realized. The method disclosed by the invention is high in yield, capable of greatly shortening the extraction time during the extraction process, capable of avoiding the influences of thermal destruction to the products, capable of reducing the production cost and applicable to large-scale production.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of active components of Chinese herbal medicines, and in particular relates to a method for extracting active components from Dendrobium officinale. Background technique [0002] Dendrobium officinale is the whole herb of the perennial epiphytic herb of Dendrobium orchidaceae. It is a precious Chinese herbal medicine, which has the functions of enhancing the body's immunity, anti-tumor, promoting the secretion of digestive juice, inhibiting platelet aggregation, lowering blood fat, lowering blood sugar, anti-oxidation, Anti-aging, antipyretic and analgesic and other pharmacological effects, its chemical composition is diverse, mainly including alkaloids, polysaccharides, amino acids, phenanthrene compounds, etc. [0003] Polysaccharides are a kind of bioactive components in Dendrobium officinale. In the pharmacological screening of the chemical components of Dendrobium officinale...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K36/8984A61P35/00A61P29/00A61P1/00A61P9/00A61P37/04
Inventor 荣龙李娇原琳荣永海
Owner BEIHANG UNIV
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