180results about How to "Optimize experimental conditions" patented technology

The invention discloses a method for extracting various active ingredients from dendrobium officinale. The method comprises the following steps of: carrying out flash-extracting; centrifuging the flash-extracted crude extracting solution; carrying out alcohol precipitation and tertiary dehydration to the centrifuged liquid supernatant to obtain free polysaccharide; combining the centrifuged precipitates with polysaccharide and alkaloid extract; and extracting the various active ingredients from the dendrobium officinale, so that the comprehensive utilization of the dendrobium officinale resources is realized. The method disclosed by the invention is high in yield, capable of greatly shortening the extraction time during the extraction process, capable of avoiding the influences of thermal destruction to the products, capable of reducing the production cost and applicable to large-scale production.

The invention discloses a kit and method for staphylococcus aureus CRISPR locus detection. The kit comprises three pairs of specific primers designed according to three CRISPR locus gene sequences of staphylococcus aureus. The invention further discloses the method for staphylococcus aureus CRISPR locus detection. The three pairs of specific primers are utilized to amplify corresponding CRISPR fragments from sample genome DNAs. The kit and the detection method are simple to operate, high in sensitivity and specificity and low in detection cost and have good popularization and application values.

The invention relates to the field of battery manufacturing and energy storage, in particular to a preparation method of a bismuth-base catalyst/carbon nanofiber combination electrode for an all-vanadium redox flow battery. Firstly, spinning solution required for an experiment is prepared and then bismuth salt and the spinning solution are uniformly mixed. According to an electrostatic spinning method, a required nanofiber film is prepared and then the nanofiber film is pre-oxidized in the air and is carbonized in an inert atmosphere tube furnace so as to obtain the required bismuth-base electrocatalyst/carbon nanofiber combination electrode. After the obtained electrode material is cleaned and dried, testing of related electrochemical performance representation and charge-discharge properties can be carried out on the electrode material. The carbon fiber diameter, which is prepared according to the preparation method, is in the nano class; compared with a specific surface area of a conventionally used electrode material, the specific surface area of the bismuth-base catalyst/carbon nanofiber combination electrode is greatly increased. Moreover, the carbon nanofibers are compounded with a high-activity bismuth-base electrocatalyst, and thus, electrochemical activity of the electrode can be greatly improved, so that energy efficiency of the all-vanadium redox flow battery is greatly improved.

The invention discloses a natural gas hydrate sediment triaxial test device, and belongs to the field of testing the mechanical properties of natural gas hydrate sediment. The device comprises a triaxial test device main machine, a temperature control system, a pore pressure control system, a confining pressure control system and a computer data acquisition and control system. The triaxial test device main machine, which is in a dual-pressure-chamber structure, can be used for accurately measuring volume change in the sediment in a natural gas hydrate decomposing process; a load sensor is arranged inside the pressure chamber and is capable of improving the precision of intensity data; a pre-cooling hollow cavity is formed inside a base to pre-cool pore gas and pore water which enter a sample, so as to relieve the decomposition of the natural gas hydrate in a saturated water process and to improve the precision of temperature control; in a sample installation process, a quick coupling is adopted for connection, so that the operation is convenient and rapid. The device can simulate the natural gas hydrate decomposing process under mining conditions, so as to achieve the high-precision temperature control, and accurate measurement of intensity and volume change; and the device has great significances for understanding the mechanical properties of the natural gas hydrate sediment and assessing the stability of a reservoir.

The invention provides a device for detecting, analyzing and processing reward operation condition reflection in real time. The device comprises a computer system and an experiment box, wherein the computer system comprises a computer host, a PCI (programmable communications interface)/CAN (controller area network) card, a graphic card, a display and an experiment control module; the graphic card is used for receiving video signals of a camera and transmitting the video signals to the computer host for processing; the display is connected with the computer host and is used for displaying experiment data; and the experiment control module is used for setting a reward experiment mode and parameters, receiving and processing the video signals and counting, recording and displaying the experiment data. With the adoption of the device for detecting, analyzing and processing the reward operation condition reflection in real time, various best experiment conditions are set according to experiment purposes; the experiment data and statistic analysis can be automatically given; the interference of external light sources on the experiment can be avoided; and the repeatability of the experiment is guaranteed.

The invention belongs to the technical field of ceramic powder preparation, and particularly relates to a preparation method of high-dispersion nano-zirconia powder. According to the method, doped yttrium metal modification, preparation of a precursor by a co-precipitation method and hydrothermal crystallization are combined to obtain the high-purity and high-dispersion nano-zirconia powder, impurity ions in the precursor are removed by an ultra-filtration composite membrane technique, crystal form control and particle dispersion functions of a mineralizer are more effectively achieved in hydrothermal reaction, the concentration of the nano-zirconia powder in the precursor is improved, and high-purity and high-yield nano-powder is prepared. The nano-zirconia powder with stable yttrium prepared by the method has a spherical particle shape, is narrow in particle size distribution, good in dispersibility, high in purity, free from aggregation and the like and can be widely applied to high-end biological ceramic materials, and the method solves the problem of brittleness of a current zirconia material in the transition process of crystal forms, so that zirconia ceramics are flexibly and widely applied.

The invention discloses a fluorescent probe for dopamine determination. The fluorescent probe for the dopamine determination is characterized in that with L-arginine as a carbon source, one-step synthesis of carbon quantum dots with strong fluorescence emission is achieved through a hydrothermal method; the carbon quantum dots are characterized by such things as ultraviolet absorption spectrum (UV), fluorescence spectrum (PL), fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), and fluorescent properties of the synthetic carbon quantum dots and the fact that the surface of the same contains a large number of functional groups such as hydroxyl groups and carbonyl groups are confirmed; according to the fluorescence quenching effect of dopamine on C-dots(carbon dots), the C-dots are used as a fluorescent probe for detecting the dopamine, and experimental conditions such as pH (hydrogen ion concentration), carbon point usage, reaction temperature andtime are optimized; a quantitative detection method for the dopamine is established under the optimal detection conditions, and the detection limit is as low as 93 nanometers, which proves that the method can be used for sensitive detection of dopamine in actual samples.

The invention discloses a universal and efficient material surface modification method which comprises the following steps: 1) preparing a dopamine solution; 2) treating a substrate surface, washing, and preserving for later use; drying a substrate material in a blowing manner before being used; putting a substrate into the dopamine solution, and drying in the blowing manner for later use; 3) grafting a PEI (Polyethyleneimine) layer on the basis of a dopamine coating, preparing a Tris*HCl solution, and sealing for preservation; preparing a polyethyleneimine (PEI) solution for later use; putting the substrate coated by a PDA layer into the polyethyleneimine (PEI) solution, sealing the opening with a preservative film, stirring at room temperature for reaction, grafting the polyethyleneimine (PEI) layer, performing reaction, taking out by using tweezers, and blowing for later use; 4) inoculating an activated initiator; 5) performing surface initiation polymerization, immobilizing the initiator on surfaces of different substrates, and preparing different types of polymer brushes on the surfaces of different substrates by using a surface initiation polymerization method. The method has the advantages of being gentle and universal.

The invention discloses an experiment device for simulating natural firing in a goaf and a test method. The experiment device is characterized in that an experiment furnace body is provided with a coal outlet, an air intake pipe and an air return pipe; a coal bed, a similar material bottom plate, a preset water bag, a to-be-settled similar material bottom plate, a first similar material top plateand a second similar material top plate are arranged in the experiment furnace body; multiple-point thermocouple testing rods and an air intake bundle pipe are arranged above the second similar material top plate; a covering layer is arranged between the multiple-point thermocouple testing rods; a plurality of thermocouples are arranged on the multiple-point thermocouple testing rods; the multiple-point thermocouple testing rods and the air intake bundle pipe are in tight contact with a similar material; both ends of a coal mining work face on the coal bed are provided with an air intake tunnel and an air return tunnel, an arch-shaped hollow protecting pipe is paved among the air intake tunnel, the coal mining work face and the air return tunnel, and a certain passage space is formed on asupport coal body, so as to build an air intake and return system for the goaf and the work face. The experiment device has the beneficial effect that the whole process of coal natural firing in the goaf under the air leakage condition can be accurately and effectively simulated in the laboratory.

The invention relates to the field of battery manufacturing and energy source storage, in particular to a preparation method of a nano graphite powder/carbon nanofiber composite electrode for an all-vanadium redox flow battery. Firstly, a spinning solution required by an experiment is prepared, and then nano graphite powder with different particle sizes and the spinning solution are uniformly mixed. By an electrospinning technology, a required nanofiber membrane is prepared, and then is pre-oxidized in the air and is carbonized in an inert atmosphere tubular atmosphere furnace to obtain a required nano graphite powder/carbon nanofiber composite electrode. According to the all-vanadium redox flow battery composite electrode prepared by the method provided by the invention, the carbon fiber diameter is at the nanoscale, and the carbon fiber is compounded with nano graphite powder with high electrical conductivity, so that the roughness of the fiber is substantially increased, and thus the specific surface area of the electrode is two orders of magnitude larger than that of the conventionally used electrode material. Meanwhile, the high-activity nano graphite powder enables the electrochemical activity of the electrode to be improved, so that the energy efficiency of the all-vanadium redox flow battery is greatly improved.

The present invention is a detection method and detecting system for the rodent sports ability, which is characterized in that the detecting system comprises an experimental box made by a metal grid bottom plate and an organic glass plate, a video camera, a computer, a computer system and detecting software. Through the video camera arranged outside the experimental box, the rodent is placed in the experimental box and the rodent is able to walk on the metal grid bottom plate. Number that the rodent fall from the metal grid bottom plate of the experimental box is counted. Therefore damage degree of the rodent sports ability is reflected. Image data of the rodent walking process is input into the computer through the video camera. The detecting software of the computer can perform the automatic identification, detection, analysis and record. The present invention improves experimental condition and provides an urgently required detection method and detecting system in the field for a long period. The present invention possesses advantages of high degree in automatic control and detection, accurate record, reliable detecting data.

The invention discloses an optical anti-counterfeiting device based on nanocrystal cellulose. The optical anti-counterfeiting device based on the nanocrystal cellulose comprises a nanocrystal cellulose film adhering to a double-sided adhesive film. A preparation method of the optical anti-counterfeiting device comprises the steps that first, a bleached fibrous material and sulfuric acid with the concentration of 64wt% are stirred at 45 DEG C for 90 min to 120 min for hydrolytic acidification, and ten-fold deionized water is added to terminate the reaction; second, centrifugal separation is carried out, concentrated suspension at the bottom is collected, the suspension is dialyzed through a dialysis membrane with the molecular weight ranging from 12000 dalton to 14000 dalton till the solution pH is equal to seven, the dialyzed suspension is made into a colloidal solution with the weight concentration ranging from 0.5wt% to 8wt% through ultrasonic dispersion; third, a fluorescent brightener is added in the colloidal solution, the mixture is evenly mixed, and the concentration of the fluorescent brightener ranges from 50 ppm to 150 ppm; fourth, the obtained colloidal solution is placed on a polystyrene petri dish and aired at room temperature so that the nanocrystal cellulose film can be obtained, and the nanocrystal cellulose film is bonded to a double-sided adhesive tape base material. The optical anti-counterfeiting device has multiple complicated dominance and recessiveness anti-counterfeiting characteristics, on-site recognition can be carried out by a simple means, and the optical anti-counterfeiting device cannot be easily counterfeited by other people.

The invention discloses an industrial control network experimental platform based on filed buses and a control method thereof, and the experimental platform comprises an experimental terminal, a remote control terminal and a communication module. The experimental terminal comprises a programmable controller which controls the field buses to transmit variable information; and the remote control terminal is used for remotely controlling the experimental terminal. The communication module comprises field bus communication units of at least two types and a field bus protocol conversion module; and the field bus protocol conversion module is arranged between the field bus communication units which are connected in series and is used for converting a data packet which can be transmitted by one bus communication unit to a data packet which can be transmitted by other bus communication units. Protocol conversion of various field buses can be realized by the field bus protocol conversion module, thus realizing data communication between two types of field buses; and information transmission among different field bus devices can be constructed in a laboratory, and students can get to know more kinds of field buses.

The invention relates to a scheme and a device, which realize the acquisition of rotation speed signals of a tested motor through a sensor, set the rotation speed signals to be in an identification range of a signal conditioning circuit, after conditioning the signals, transmit the signals into a computer through a data acquisition card, finish processing data through virtual instrument software in the computer, display digital values of a rotation speed, torque and output power obtained through analysis processing on a friendly human-computer interface of the computer and simultaneously record the digital values in corresponding files. The scheme and the device have the following advantages that: experimental conditions for direct current machine testing in the prior art are improved; the whole scheme has good repeatability and avoids personal errors; the device applying the scheme realizes the integration of experimental instruments, so that the integration cost of experimental and testing equipment is remarkably reduced and steps are remarkably reduced; and through proper extension, the scheme and the device can be widely applied to the experiment teaching of other subjects, and can realize the experiment teaching and scientific research of a network and greatly improve the practical efficiency of experimental environments.

The invention discloses a weak blast-induced strain-type rock-burst site simulation test method. The method comprises the steps of digging a test branch cave and a monitoring branch cave in the maximum burial depth section of a deep-buried tunnel, determining a stress concentration region in surrounding rock of the monitoring branch cave by virtue of numerical simulation, drilling blast holes in the stress concentration region of the surrounding rock, filling the blast holes with explosives at the bottoms, simultaneously setting vibration and dynamic strain test holes, generating a weak blast to induce strain energy accumulated by rock masses of the stress concentration region to release to form a simulated rock blast, and carrying out dynamic strain and vibration testing on a dynamic effect of the rock burst during the test. According to the method, by analyzing actually measured vibration and dynamic strain data of the rock masses, the internal relation among the energy release speed, the magnitude of induced vibration and the rock burst intensity during the rock bursting is revealed, to facilitate the prediction, prevention and control of the rock bursting; and the method can be widely applied to the fields of hydraulic and hydropower engineering, traffic, mines, national strategic defense and deep basic physical experiments and has important significances in the prediction, prevention and control of the rock bursting and the guarantee of construction safety of underground cavities.

A physicochemical experiment system based on virtual instrument control technique is composed of a temp controller on the basis of semiconductor refrigerating technique, a signal processing unit connected via 485 bus to said temp controller, computer and software control system.

The invention discloses a preparation method and application of a nitrogen-doped porous carbon fiber and belongs to the technical field of preparation of porous carbon fiber materials. The preparationmethod comprises the following steps of firstly, preparing a polyacrylonitrile/polyvinylpyrrolidone spinning precursor solution, then obtaining a porous polyacrylonitrile fiber through a wet spinningmethod by utilizing different solubilities (phase separation) of the polyacrylonitrile and the polyvinylpyrrolidone in an aqueous solution, soaking and activating the obtained porous polyacrylonitrile fiber in a potassium hydroxide solution, taking the polyacrylonitrile as a carbon source and a nitrogen source, and carrying out one-step high-temperature carbonization activation treatment in an inert atmosphere to obtain the nitrogen-doped porous carbon fiber. The porous carbon fiber material has a hierarchical porous carbon skeleton structure with high specific surface area, high nitrogen content and mutual crosslinking, a fast channel is provided for electron transmission, the ion diffusion distance is shortened, the wettability and conductivity of the material are increased, and good electrochemical performance is shown. The preparation process is simple, the equipment dependence is low, and the method is suitable for large-scale industrial production.