The invention relates to an experimental animal sign information acquisition and analysis system and a collection and analysis method thereof, which are characterized by comprising a hardware system, a software system and an implementation method. The hardware system comprises a light source, a shading component, a digital camera, a stepper motor for controlling the digital camera to move, a connecting device and a computer. The software system comprises a digital camera moving and shooting control program, a database program, and an image analysis program. The implementation method is that: experimenters operate the system to complete the collection and analysis of data according to instructions. The image analysis program also comprises a tongue image automatic collection and analysis module, a whole hair color collection and analysis module, an ear collection and analysis module, a claw collection and analysis module, and a tail collection and analysis module. The analysis method comprises the following steps of automatic image storage, smart-cut of analysis target images, and automatic extraction and storage of sign data. The system has a scientific design and high degree of automation, is convenient to use, and can be used for a collection and analysis research of various experimental animal sign images, and the obtained experimental data is objective, scientific, and reliable.