313results about How to "Mild extraction conditions" patented technology

The invention discloses a process for extraction of astaxanthin in Haematococcus pluvialis. According to the process, wall-broken Haematococcus pluvialis is obtained by using an acid method, and then an astaxanthin extract is obtained through ethanol dehydration and organic solvent extraction. The wall breaking and extraction process provided by the invention has the advantages of easiness, high efficiency, convenient operation, low energy consumption and controllable quality, and the wall breaking rate and the extraction ratio of Haematococcus pluvialis can reach more than 95%. The astaxanthin extract prepared by using the process is a dark red oily viscous substance and can meet requirements for raw materials needed in production of health food, cosmetics and medicines.

The invention discloses a method for extracting various active ingredients from dendrobium officinale. The method comprises the following steps of: carrying out flash-extracting; centrifuging the flash-extracted crude extracting solution; carrying out alcohol precipitation and tertiary dehydration to the centrifuged liquid supernatant to obtain free polysaccharide; combining the centrifuged precipitates with polysaccharide and alkaloid extract; and extracting the various active ingredients from the dendrobium officinale, so that the comprehensive utilization of the dendrobium officinale resources is realized. The method disclosed by the invention is high in yield, capable of greatly shortening the extraction time during the extraction process, capable of avoiding the influences of thermal destruction to the products, capable of reducing the production cost and applicable to large-scale production.

The invention provides a method and a device for extracting a fat-soluble natural pigment. The method comprises the following steps of: (1) performing pretreatment on a material, and then performing pressure reduction treatment on the treated material in an extraction container; (2) introducing a solvent into the extraction container to mix the solvent and the material, and extracting the mixture to obtain extract liquor; (3) putting the extract liquor into a vaporization separation device to separate out a crude extract of the fat-soluble natural pigment, and simultaneously separating the solvent, compressing and cooling the solvent, and then reclaiming the solvent for cyclic utilization; and (4) putting the crude extract of the fat-soluble natural pigment into a refining system to perform refining treatment to prepare a refined extract. The extraction device comprises an extraction separation system, the refining system, a solvent purification system, a vacuum system and a heat exchange system. The method and the device can improve the oxidation resistance and the stability of a product, and ensure the biological and chemical activities of the extracts; the product has pure and stable colors; the extraction rate is high; the solvent residue is low; the product has no peculiar smells; and the solvent is easy to reclaim for cyclic utilization.

The invention relates to a method for continuously extracting functional components of chlorella vulgaris, which comprises the steps of enriching the chlorella vulgaris through the membrane separation technology, obtaining concentrated slurry of the chlorella vulgaris, adding a proper enzyme in a liquid phase system for wall-breaking, and using a solvent for extracting chlorophyll from chlorella vulgaris solution after wall-breaking, thereby being capable of obtaining a functional pigment product and reducing the color value of follow-up products; and firstly carrying out enzymatic hydrolysis on a water phase of the chlorella vulgaris after decoloring, then using the solvent for extracting functional grease, extracting active polysaccharides from the obtained degreased chlorella vulgaris by using the acid method, and then drying the other parts for obtaining crude proteins of the chlorella vulgaris. The main products comprise the chlorophyll, the active polysaccharides of the chlorella vulgaris, the functional grease of the chlorella vulgaris, the proteins of the chlorella vulgaris and the like, and the method optimizes the process on the basis of ensuring the activity of the functional components of the chlorella vulgaris, thereby obtaining high yield and reducing production cost.

The invention relates to the technical field of preparation of extracting agents and deep-eutectic solvents, and relates to application of an extracting agent and a deep-eutectic solvent in determining of effective ingredients in traditional Chinese medicine, in particular to application in determining of flavonoid ingredient in radix scutellariae, and application of an extracting agent and a deep-eutectic solvent in determining of effective ingredients of baicalin, baicalein, wogonoside, wogonin and scutellarin in radix scutellariae. The application is characterized in that the radix scutellariae is used as the raw material, the deep-eutectic solvent, especially choline deep-eutectic solvent, is used as the extracting agent, the water is used as a thinner for reducing the system viscosity, and the flavonoid compound is forcibly extracted under the ultrasonic condition. An extracting method has the advantages that the extracting efficiency of the flavonoid ingredient is higher, the extracting conditions are moderate, and the operation is simple and convenient. The extracting agent has the characteristics of no burning explosion and volatizing, small loss, low energy consumption andthe like. The extracting method is also suitable for extracting the flavonoid-containing traditional Chinese medicines, such as radix astragali seu hedysari, liquorice root, radix puerariae and radixsophorae flavescentis.

The invention discloses a method for extracting blueberry seed oil by adopting an ultrasonic-assisted aqueous enzymatic method. The method for extracting the blueberry seed oil by adopting the ultrasonic-assisted aqueous enzymatic method comprises the following steps: carrying out breaking, pulverization and then enzymolysis treatment on blueberry seed taken as raw material, applying certain ultrasonic for assisting extraction in different stages, then carrying out enzyme deactivation and centrifugation, and taking a supernate, so that the blueberry seed oil is obtained. The method for extracting the blueberry seed oil is simple in operation, mild in conditions, high in oil yield, pollution-free, easy for refining, low in production cost and applicable to large-scale production.

The present invention discloses technology of enzymolyzing waste bamboo shoot coat to prepared functional diet fiber. Waste bamboo shoot coat, after being washed, dried, crushed and defatted, is added into pH 4.5-5.5 phosphate buffering solution in the concentration of 15-20 ml/g with cellulolytic enzyme in enzyme activity of 5-10 U/ml and enzyme concentration of 0.5-1 % to enzymolyze at 100-120 rpm and 50-60 deg.c for 1-2 hr, before decolorizing and vacuum drying to obtain functional diet fiber. The simple technological process can utilize the fiber resource in waste bamboo shoot coat effectively, and the functional diet fiber product high soluble diet fiber content.

The invention discloses a sample pretreatment method for detection of organochlorine pesticide residue in food and is characterized by comprising the following steps: water is added to a food sample, sample solution is swirled, and then inorganic salt and hydrophilic organic solvent are added to the sample solution, the sample solution is swirled and shaken uniformly, the sample solution undergoes phase splitting after centrifugation at room temperature, the upper-phase liquid is purified through dispersion solid-phase extraction method, the extraction temperature is 20-30 DEG C, and finally the upper-phase liquid is detected by gas chromatography; and the mass percent of the inorganic salt accounts for 5%-35% of the total mass of the system, the mass percent of the hydrophilic organic solvent accounts for 10%-50% of the total mass of the system, and water is of the residual amount, and the total mass of the system is the mass sum of water contained in the sample, the added water, the inorganic salt and the hydrophilic organic solvent. According to the sample pretreatment method, a dual-water-phase system consisting of the hydrophilic organic solvent, the organic salt and water is utilized, the partition coefficient is increased by means of changing the types and concentrations of the organic solvent and the inorganic salt, and the organochlorine pesticide residue in the sample can be directly extracted at room temperature.

The industrial extracting process of high purity lecithin includes mixing the material and alcohol solvent in the weight ratio of 0.1-0.2 at 50-75 deg.c in a constant temperature container, stirring to extract for 15-60 min, evaporating at 25-50 deg.c to concentrate for 20-60 min, decompressing at 35-45 deg.c to eliminate solvent, separating and purifying with methanol as the eluent in the flow rate of 60-120 ml/min, concentrating the eluted liquid and vacuum freeze drying to obtain high purity lecithin. The present invention is suitable for use industrial production.

The invention relates to a method for extracting a silk protein. At present, a method for extracting the silk protein, in which the extraction condition is mild and which facilitates reducing the influence of the extraction condition on the structure and performance of the silk protein, is not provided. The method comprises the following steps of: placing the raw materials for preparing the silk protein in a CaCl2 aqueous solution at the temperature of between 95 and 100 DEG C, and dissolving until an undissolved part is jelly to obtain a dissolution treatment solution; filtering the dissolution treatment solution by using filter paper to obtain a silk protein/CaCl2 mixed aqueous solution and the undissolved jelly; washing the undissolved jelly to obtain silk protein colloid; and/or placing the silk protein/CaCl2 mixed aqueous solution in a dialysis bag and dialyzing and purifying to obtain a silk protein solution; and processing the silk protein colloid and/or the silk protein solution to obtain a finished product silk protein. According to the method, the extraction condition is mild, the extraction efficiency of the silk protein is improved conveniently, the waste of the silk protein resources is reduced, and the influence of the extraction condition on the structure and performance on the silk protein is reduced.

The invention discloses a mixture of momordica saponins and polysaccharides, which is obtained by the following preparation methods: the balsam pear is used as a raw material and is added with an organic solvent to undergo ultrasound-microwave assisted extraction; a momordica saponins extract is obtained after filtration; momordica filter residues are extracted in alkaline solution; the obtained extract is namely the momordica polysaccharides extract; the momordica saponins extract and the momordica polysaccharides extract are respectively concentrated, dried and mixed to obtain a mixture of momordica polysaccharides and saponins; the invention also discloses an extraction process and application of the mixture of the momordica saponins and polysaccharides; the mixture has various physiological functions such as blood sugar level reduction, cancer resistance, virus resistance, blood lipids regulation, immunity increasing and the like; the mixture can be used for preparing health food with the function of reducing blood sugar level; the extraction process adopts step-by-step extraction technology and has the characteristics of high raw materials utilization rate, mild extraction conditions, high speed, high efficiency and the like; compared with the single extraction technology, for the extraction process of the invention, the extraction time is shortened by more than 20% and the purity of the product is improved by more than 15%.

The present invention discloses the technological process of enzymolyzing bayberry fruit dregs to prepare functional diet fiber. The technological process includes the following steps: defatting bayberry fruit dregs, adding pH 4.5-5.5 phosphate buffering solution in 15-20 ml/g and 0.1-0.5 % enzyme concentration and 5-10 U/ml enzyme activity cellulolytic enzyme solution, enzymolysis at 100-120 rpm and 55-60 deg.c for 1-2 hr, alcohol separating, and vacuum drying to obtain functional diet fiber. The simple technological process changes waste into valuable functional diet fiber and is environment friendly.

The invention discloses a method for extracting and separating ganoderma lucidum polysaccharide from ganoderma lucidum sporocarp. The method comprises the steps of carrying out enzymolysis and extraction with complex enzyme on the ganoderma lucidum sporocarp to obtain enzymolysis solution, concentrating and separating the enzymolysis solution through a tangential flow filtering membrane to obtainultrafiltration retention solution, carrying out elution, separation and purification on the ultrafiltration retention solution with purified water through radial flow chromatograph to obtain polysaccharide eluent, and conducting reduced pressure concentration and drying on the polysaccharide eluent to obtain ganoderma lucidum polysaccharide, wherein the papain and and cellulose are mixed in a weight ratio of 1:2-4:1 to form complex enzyme. The invention builds a set of polysaccharide production process of ganoderma lucidum sporocarp from raw materials to products, the process is environment-friendly, fast and efficient and suitable for large-scale production and the process cost for refining ganoderma lucidum polysaccharide can be greatly reduced.

The invention discloses a sesame oil extraction method. The method comprises the following steps that 1, sesame seeds are cleaned, husked, smashed, subjected to water regulation and then subjected to freezing treatment; 2, microwave defrosting is conducted on the sesame powder subjected to freezing treatment; 3, the defrosted sesame powder is subjected to step-by-step enzymolysis, centrifugal separation is conducted after enzymolysis is completed, and sesame oil, emulsion, hydrolysate and residues are obtained; 4, the emulsion is subjected to ultrasonic treatment, ethyl alcohol is added, centrifugation is conducted, and the sesame oil is obtained. According to the sesame oil extraction method, the high-quality green sesame oil can be obtained, needed equipment is simple, operation is safe, the obtained sesame oil is free of residual solvent, and the final total oil extraction rate can reach 97.91%.

The invention relates to the technical field of traditional Chinese medicine extraction, and relates to a method for extracting flavonoid glycoside compounds in herba epimedii, in particular to a method for extracting flavonoid glycoside compounds in epimedium leaves with a deep eutectic solvent. According to the preparation method, the flavonoid glycoside compounds are extracted from the raw material of herba epimedii with the deep eutectic solvent, particularly a choline deep eutectic solvent, as an extracting agent and water as a diluent for reducing the system viscosity under ultrasonic enhancement. The extraction method has higher extraction efficiency for flavonoid glycoside components, is more thorough in extraction and has the extraction efficiency close to 100%. The method is mildin extraction condition and convenient to operate, has the characteristics of no blast or volatilization of the extracting agent, low loss, low energy consumption and the like and can be applied in efficient and green large-scale production of the flavonoid glycoside compounds and comprehensive utilization of the herba epimedii resource.

The invention discloses a method for extracting cellulose from manioc waste. The method comprises the following steps: washing manioc waste, drying and smashing; adding water to the grinded manioc waste, boiling till the water is in a boiling state, so that starch in the manioc waste becomes pasty, then adding with enzyme, carrying out enzymolysis under ultrasound, deactivating enzyme, centrifuging, and removing the supernate; adding a sodium hydroxide solution into the residue for alkaline hydrolysis, washing, filtering, and drying, and thus obtaining manioc waste cellulose. The invention further discloses a method for preparing a heavy metal adsorption material by adopting the manioc waste cellulose as the raw material, and an adsorption method of heavy metal ions. The manioc waste is taken as a starting material, the cellulose is extracted from the manioc waste through a method combining bio-enzyme and alkaline hydrolysis, the manioc waste cellulose with high content can be rapidly and efficiently obtained, the waste is turned into valuable, the obtained heavy metal adsorption material has great adsorption capacity for the heavy metal ions in water, especially has an excellent adsorption effect for cadmium and lead in the water, and therefore, the manioc waste cellulose has good economy and environment protection value.

The invention discloses a method for extracting effective components in holothurian by means of salting out, belonging to the technical field of the further processing of marine products. The method comprises the following steps of: directly adding some electrolyte and common organic solvent into holothurian extracting liquid to form into an organic solvent/salt multiphase salting out and extracting system with the hydration of salt ion; separating and concentrating the effective components according to the difference of solubility and the different distribution of the different effective components with different polarities in the holothurian in each phase; adding salt and hydrophilic low-molecular organic matter into holothurian extract liquid according to a certain proportion, evenly stirring, stewing or decentralizing to form into double water phase; and adding the salt, the hydrophilic low-molecular organic matter and the hydrophobic organic matter into the holothurian extracting liquid, evenly stirring, stewing or decentralizing to form into a three-liquid phase salting out extracting system. The method develops a novel salting out extracting method which can simultaneously distill and separate various effective components in the holothurian, is simple in operation, moderate in condition and low in cost, and effectively reduces the toxic action of heavy metal.

The invention discloses an extraction method of wolfberry polysaccharide, which comprises the following steps of: (1) arranging a wolfberry powder in an extractor, adding petroleum ether into the extractor to carry out extraction, and drying the extract to obtain a degreasing powder; (2) preparing a cellulase solution by using cellulase; (3) dissolving the degreasing powder in the cellulase solution, and carrying out ultrasound extraction; (4) heating the extract to kill enzyme, removing solids by carrying out air pump filtration on the extract after cooling the extract until room temperature to obtain an extracting solution; (5) carrying out evaporation on the extracting solution, and carrying out concentration to obtain a concentrated solution; (6) carrying out alcohol precipitation; and (7) carrying out centrifugation on an alcohol precipitation fluid, taking out a precipitate, washing the precipitate by absolute ethyl alcohol, and carrying out freeze-drying on the precipitate to obtain the wolfberry polysaccharide. With the adoption of the extraction method, the yield of the wolfberry polysaccharide is high, can reach 6.32 percent and is improved by 62.6 percent compared with the traditional hot water extraction method; the extraction time is greatly shortened and is one ninth of time needed by the hot water extraction method, and therefore, the extraction efficiency is improved; and the extraction temperature is lowered, the extraction condition is mild, and the stereochemical structure and the biological activity of the wolfberry polysaccharide can be effectively protected.

A white kidney bean extract extracted by microwave is disclosed, wherein the bioactivity of an alpha-amylase inhibitor contained in the extract is equal to or higher than 4100 U/mg, and the HPLC (high-performance liquid chromatography) content is equal to or higher than 20% w/w. Preparation method for the extract is characterized by comprising the following steps of: crushing white kidney beans and passing through a 60-mesh sieve; preparing a mixture which is uniformly mixed by the crushed and sieved white kidney bean powder and purified water in a weight ratio of 1:5, extracting for 1-10 minutes at a temperature below 40 DEG C in microwave extraction equipment, then filtering to obtain a filtrate, and removing the filter residue; heating the filtrate to 70 DEG C in a water bath with stirring, keeping for 20 minutes, and collecting an effluent through a silica gel column; and drying the effluent to prepare the white kidney bean extract. The extract can be used for preparing an appetite inhibitor. The preparation method disclosed by the invention shortens extraction time and effectively increases extraction rate. The purified water is used as an extraction solvent, as well as is safe and environment-friendly.

The invention discloses a method for extracting and separating curcumin and curcuma oil from carcuma longa, which comprises the following steps of: grinding the carcuma longa into carcuma longa powder with particle size of 80-100 meshes; taking ethanol water solution as extractant for dynamic countercurrent extraction of the carcuma longa powder to obtain extraction solution, and concentrating to obtain concentrated solution; and adding the concentrated solution with plant oil extraction solvent for liquid-liquid extraction to obtain light phase containing curcuma oil and heavy phase containing curcumin. Compared with the prior art, the invention takes the ethanol water solution as the extractant and extracts the curcumin and the curcuma oil from the carcuma longa by employing the dynamic countercurrent extraction method, and the method has the advantages of simple operation, moderate extraction condition, low usage amount of solvent, short extraction time, few impurities in extraction solution and high extraction efficiency.

The invention provides a process for extracting chondroitin sulfate from pigs, which comprises: raw material treatment, enzymolysis, absorption decolorizing, salting of protein, alcohol precipitation and crystallization, vacuum drying and the like. In method disclosed by the invention, the extraction rate of chondroitin sulfate is taken as an index, a static optimizing method combining single factor experiment, factorial experiment and response overall analysis experiment is adopted to optimize the extraction process, the process conditions are reasonable, and the effect is very stable. The method has the advantages of extracting the chondroitin sulfate from pig gristles by one step of enzymolysis reaction, ensuring white product color and high product purity, avoiding pollution caused by alkaline extraction, contributing to environment protection, making industrial production easy, along with mild extraction conditions, short production period, and high extraction rate.

The invention relates to the field of jerusalem artichoke processing and utilization and in particular relates to a method for extracting high-purity inulin from jerusalem artichoke by an ultrasonic-complex enzyme method, solving the problem that existing processes for extracting inulin from jerusalem artichoke and processes for purification respectively have various defects. The method comprises an extraction procedure and a purification procedure, wherein the extraction procedure comprises the steps of slicing, drying and grinding tubers of jerusalem artichoke, adding water to dissolve the powder, then adding a complex enzyme and putting the mixture into an ultrasonic washing unit to undergo ultrasonic extraction; carrying out enzyme deactivation and filtration, thus obtaining coarse inulin extract; the purification procedure comprises the steps of forming a dynamic cascade resin chromatographic column with resins D280 and D151; firstly subjecting the coarse inulin extract to the resin D280, then subjecting the coarse inulin extract to the resin D151 and collecting the effluent; subjecting the collected fluid to an ultrafiltration membrane with molecular weight cutoff of 10KDa and a nanofiltration membrane with molecular weight cutoff of 500Da respectively and drying and grinding the cutoff fluid into powder, thus obtaining inulin. The method has the beneficial effects that the inulin yield is greatly increased, the extraction time and temperature are obviously reduced, the inulin yield is high, the product properties and quality are stable and the aims of large-scale production, high yield and good quality are reached.