313results about How to "Mild extraction conditions" patented technology

The invention discloses a process for extraction of astaxanthin in Haematococcus pluvialis. According to the process, wall-broken Haematococcus pluvialis is obtained by using an acid method, and then an astaxanthin extract is obtained through ethanol dehydration and organic solvent extraction. The wall breaking and extraction process provided by the invention has the advantages of easiness, high efficiency, convenient operation, low energy consumption and controllable quality, and the wall breaking rate and the extraction ratio of Haematococcus pluvialis can reach more than 95%. The astaxanthin extract prepared by using the process is a dark red oily viscous substance and can meet requirements for raw materials needed in production of health food, cosmetics and medicines.

The invention relates to a method for preparing industrial grade and food grade phosphoric acid by decomposing low grade phosphate rocks with hydrochloric acid. The method comprises the following processing steps: (1) decomposing phosphate rock powder with hydrochloric acid; (2) extracting to remove iron; (3) preparing dilute phosphoric acid; (4) preparing industrial grade phosphoric acid; and (5) preparing food grade phosphoric acid. Food grade phosphoric acid can be prepared only by adding deep defluorination process on the basis of the preparation steps of industrial grade phosphoric acid. The deep defluorination process adopts vacuum steam stripping process and the processing conditions are as follows: the vacuum degree is 0.075-0.090MPa, and the temperature is 95-110 DEG C. Industrial grade phosphoric acid prepared by the method of the invention meets the super grade or first-class product standards of GB / T 2091-2008; and food grade phosphoric acid meets the standards of GB 3149-2004.

The invention discloses a method for extracting various active ingredients from dendrobium officinale. The method comprises the following steps of: carrying out flash-extracting; centrifuging the flash-extracted crude extracting solution; carrying out alcohol precipitation and tertiary dehydration to the centrifuged liquid supernatant to obtain free polysaccharide; combining the centrifuged precipitates with polysaccharide and alkaloid extract; and extracting the various active ingredients from the dendrobium officinale, so that the comprehensive utilization of the dendrobium officinale resources is realized. The method disclosed by the invention is high in yield, capable of greatly shortening the extraction time during the extraction process, capable of avoiding the influences of thermal destruction to the products, capable of reducing the production cost and applicable to large-scale production.

The invention provides a method and a device for extracting a fat-soluble natural pigment. The method comprises the following steps of: (1) performing pretreatment on a material, and then performing pressure reduction treatment on the treated material in an extraction container; (2) introducing a solvent into the extraction container to mix the solvent and the material, and extracting the mixture to obtain extract liquor; (3) putting the extract liquor into a vaporization separation device to separate out a crude extract of the fat-soluble natural pigment, and simultaneously separating the solvent, compressing and cooling the solvent, and then reclaiming the solvent for cyclic utilization; and (4) putting the crude extract of the fat-soluble natural pigment into a refining system to perform refining treatment to prepare a refined extract. The extraction device comprises an extraction separation system, the refining system, a solvent purification system, a vacuum system and a heat exchange system. The method and the device can improve the oxidation resistance and the stability of a product, and ensure the biological and chemical activities of the extracts; the product has pure and stable colors; the extraction rate is high; the solvent residue is low; the product has no peculiar smells; and the solvent is easy to reclaim for cyclic utilization.

The invention relates to a method for continuously extracting functional components of chlorella vulgaris, which comprises the steps of enriching the chlorella vulgaris through the membrane separation technology, obtaining concentrated slurry of the chlorella vulgaris, adding a proper enzyme in a liquid phase system for wall-breaking, and using a solvent for extracting chlorophyll from chlorella vulgaris solution after wall-breaking, thereby being capable of obtaining a functional pigment product and reducing the color value of follow-up products; and firstly carrying out enzymatic hydrolysis on a water phase of the chlorella vulgaris after decoloring, then using the solvent for extracting functional grease, extracting active polysaccharides from the obtained degreased chlorella vulgaris by using the acid method, and then drying the other parts for obtaining crude proteins of the chlorella vulgaris. The main products comprise the chlorophyll, the active polysaccharides of the chlorella vulgaris, the functional grease of the chlorella vulgaris, the proteins of the chlorella vulgaris and the like, and the method optimizes the process on the basis of ensuring the activity of the functional components of the chlorella vulgaris, thereby obtaining high yield and reducing production cost.

The invention discloses a method for extracting antarctic krill grease and separating other biological active substances, and belongs to the technical field of deep processing of marine food products. Aiming at the characteristics that the water content of antarctic krill is high and the antarctic krill is easy to autolyze, the grease can be directly extracted from the frozen antarctic krill at low temperature by using hydrophobic and hydrophilic mixed solvents; a salt solution and a common organic solvent are added into residues and an organic solvent / salt multi-phase salting-out extracting system is formed by using salting-out extracting effect; the distributions of biological active substances with different polarities in the antarctic krill in each phase are different according to a solubility difference; and furthermore, the residual grease is extracted to obtain astaxanthin; and shrimp meat proteins are separated and the solvents in the residues are recycled. The method disclosed by the invention is simple in operation and easy to realize, and has low cost, short period and moderate extracting conditions, and the organic solvents are cheap and easy to recycle, so that the method is easy to industrialize.

The invention relates to the technical field of preparation of extracting agents and deep-eutectic solvents, and relates to application of an extracting agent and a deep-eutectic solvent in determining of effective ingredients in traditional Chinese medicine, in particular to application in determining of flavonoid ingredient in radix scutellariae, and application of an extracting agent and a deep-eutectic solvent in determining of effective ingredients of baicalin, baicalein, wogonoside, wogonin and scutellarin in radix scutellariae. The application is characterized in that the radix scutellariae is used as the raw material, the deep-eutectic solvent, especially choline deep-eutectic solvent, is used as the extracting agent, the water is used as a thinner for reducing the system viscosity, and the flavonoid compound is forcibly extracted under the ultrasonic condition. An extracting method has the advantages that the extracting efficiency of the flavonoid ingredient is higher, the extracting conditions are moderate, and the operation is simple and convenient. The extracting agent has the characteristics of no burning explosion and volatizing, small loss, low energy consumption andthe like. The extracting method is also suitable for extracting the flavonoid-containing traditional Chinese medicines, such as radix astragali seu hedysari, liquorice root, radix puerariae and radixsophorae flavescentis.

The invention discloses a method for extracting blueberry seed oil by adopting an ultrasonic-assisted aqueous enzymatic method. The method for extracting the blueberry seed oil by adopting the ultrasonic-assisted aqueous enzymatic method comprises the following steps: carrying out breaking, pulverization and then enzymolysis treatment on blueberry seed taken as raw material, applying certain ultrasonic for assisting extraction in different stages, then carrying out enzyme deactivation and centrifugation, and taking a supernate, so that the blueberry seed oil is obtained. The method for extracting the blueberry seed oil is simple in operation, mild in conditions, high in oil yield, pollution-free, easy for refining, low in production cost and applicable to large-scale production.

The present invention discloses technology of enzymolyzing waste bamboo shoot coat to prepared functional diet fiber. Waste bamboo shoot coat, after being washed, dried, crushed and defatted, is added into pH 4.5-5.5 phosphate buffering solution in the concentration of 15-20 ml / g with cellulolytic enzyme in enzyme activity of 5-10 U / ml and enzyme concentration of 0.5-1 % to enzymolyze at 100-120 rpm and 50-60 deg.c for 1-2 hr, before decolorizing and vacuum drying to obtain functional diet fiber. The simple technological process can utilize the fiber resource in waste bamboo shoot coat effectively, and the functional diet fiber product high soluble diet fiber content.

The invention discloses a sample pretreatment method for detection of organochlorine pesticide residue in food and is characterized by comprising the following steps: water is added to a food sample, sample solution is swirled, and then inorganic salt and hydrophilic organic solvent are added to the sample solution, the sample solution is swirled and shaken uniformly, the sample solution undergoes phase splitting after centrifugation at room temperature, the upper-phase liquid is purified through dispersion solid-phase extraction method, the extraction temperature is 20-30 DEG C, and finally the upper-phase liquid is detected by gas chromatography; and the mass percent of the inorganic salt accounts for 5%-35% of the total mass of the system, the mass percent of the hydrophilic organic solvent accounts for 10%-50% of the total mass of the system, and water is of the residual amount, and the total mass of the system is the mass sum of water contained in the sample, the added water, the inorganic salt and the hydrophilic organic solvent. According to the sample pretreatment method, a dual-water-phase system consisting of the hydrophilic organic solvent, the organic salt and water is utilized, the partition coefficient is increased by means of changing the types and concentrations of the organic solvent and the inorganic salt, and the organochlorine pesticide residue in the sample can be directly extracted at room temperature.

The industrial extracting process of high purity lecithin includes mixing the material and alcohol solvent in the weight ratio of 0.1-0.2 at 50-75 deg.c in a constant temperature container, stirring to extract for 15-60 min, evaporating at 25-50 deg.c to concentrate for 20-60 min, decompressing at 35-45 deg.c to eliminate solvent, separating and purifying with methanol as the eluent in the flow rate of 60-120 ml / min, concentrating the eluted liquid and vacuum freeze drying to obtain high purity lecithin. The present invention is suitable for use industrial production.

The invention relates to a method for extracting a silk protein. At present, a method for extracting the silk protein, in which the extraction condition is mild and which facilitates reducing the influence of the extraction condition on the structure and performance of the silk protein, is not provided. The method comprises the following steps of: placing the raw materials for preparing the silk protein in a CaCl2 aqueous solution at the temperature of between 95 and 100 DEG C, and dissolving until an undissolved part is jelly to obtain a dissolution treatment solution; filtering the dissolution treatment solution by using filter paper to obtain a silk protein / CaCl2 mixed aqueous solution and the undissolved jelly; washing the undissolved jelly to obtain silk protein colloid; and / or placing the silk protein / CaCl2 mixed aqueous solution in a dialysis bag and dialyzing and purifying to obtain a silk protein solution; and processing the silk protein colloid and / or the silk protein solution to obtain a finished product silk protein. According to the method, the extraction condition is mild, the extraction efficiency of the silk protein is improved conveniently, the waste of the silk protein resources is reduced, and the influence of the extraction condition on the structure and performance on the silk protein is reduced.

The invention discloses a mixture of momordica saponins and polysaccharides, which is obtained by the following preparation methods: the balsam pear is used as a raw material and is added with an organic solvent to undergo ultrasound-microwave assisted extraction; a momordica saponins extract is obtained after filtration; momordica filter residues are extracted in alkaline solution; the obtained extract is namely the momordica polysaccharides extract; the momordica saponins extract and the momordica polysaccharides extract are respectively concentrated, dried and mixed to obtain a mixture of momordica polysaccharides and saponins; the invention also discloses an extraction process and application of the mixture of the momordica saponins and polysaccharides; the mixture has various physiological functions such as blood sugar level reduction, cancer resistance, virus resistance, blood lipids regulation, immunity increasing and the like; the mixture can be used for preparing health food with the function of reducing blood sugar level; the extraction process adopts step-by-step extraction technology and has the characteristics of high raw materials utilization rate, mild extraction conditions, high speed, high efficiency and the like; compared with the single extraction technology, for the extraction process of the invention, the extraction time is shortened by more than 20% and the purity of the product is improved by more than 15%.

The present invention discloses the technological process of enzymolyzing bayberry fruit dregs to prepare functional diet fiber. The technological process includes the following steps: defatting bayberry fruit dregs, adding pH 4.5-5.5 phosphate buffering solution in 15-20 ml / g and 0.1-0.5 % enzyme concentration and 5-10 U / ml enzyme activity cellulolytic enzyme solution, enzymolysis at 100-120 rpm and 55-60 deg.c for 1-2 hr, alcohol separating, and vacuum drying to obtain functional diet fiber. The simple technological process changes waste into valuable functional diet fiber and is environment friendly.

The invention discloses a method for extracting and separating ganoderma lucidum polysaccharide from ganoderma lucidum sporocarp. The method comprises the steps of carrying out enzymolysis and extraction with complex enzyme on the ganoderma lucidum sporocarp to obtain enzymolysis solution, concentrating and separating the enzymolysis solution through a tangential flow filtering membrane to obtainultrafiltration retention solution, carrying out elution, separation and purification on the ultrafiltration retention solution with purified water through radial flow chromatograph to obtain polysaccharide eluent, and conducting reduced pressure concentration and drying on the polysaccharide eluent to obtain ganoderma lucidum polysaccharide, wherein the papain and and cellulose are mixed in a weight ratio of 1:2-4:1 to form complex enzyme. The invention builds a set of polysaccharide production process of ganoderma lucidum sporocarp from raw materials to products, the process is environment-friendly, fast and efficient and suitable for large-scale production and the process cost for refining ganoderma lucidum polysaccharide can be greatly reduced.

The invention discloses a sesame oil extraction method. The method comprises the following steps that 1, sesame seeds are cleaned, husked, smashed, subjected to water regulation and then subjected to freezing treatment; 2, microwave defrosting is conducted on the sesame powder subjected to freezing treatment; 3, the defrosted sesame powder is subjected to step-by-step enzymolysis, centrifugal separation is conducted after enzymolysis is completed, and sesame oil, emulsion, hydrolysate and residues are obtained; 4, the emulsion is subjected to ultrasonic treatment, ethyl alcohol is added, centrifugation is conducted, and the sesame oil is obtained. According to the sesame oil extraction method, the high-quality green sesame oil can be obtained, needed equipment is simple, operation is safe, the obtained sesame oil is free of residual solvent, and the final total oil extraction rate can reach 97.91%.

The invention relates to the technical field of traditional Chinese medicine extraction, and relates to a method for extracting flavonoid glycoside compounds in herba epimedii, in particular to a method for extracting flavonoid glycoside compounds in epimedium leaves with a deep eutectic solvent. According to the preparation method, the flavonoid glycoside compounds are extracted from the raw material of herba epimedii with the deep eutectic solvent, particularly a choline deep eutectic solvent, as an extracting agent and water as a diluent for reducing the system viscosity under ultrasonic enhancement. The extraction method has higher extraction efficiency for flavonoid glycoside components, is more thorough in extraction and has the extraction efficiency close to 100%. The method is mildin extraction condition and convenient to operate, has the characteristics of no blast or volatilization of the extracting agent, low loss, low energy consumption and the like and can be applied in efficient and green large-scale production of the flavonoid glycoside compounds and comprehensive utilization of the herba epimedii resource.

The invention discloses a method for extracting cellulose from manioc waste. The method comprises the following steps: washing manioc waste, drying and smashing; adding water to the grinded manioc waste, boiling till the water is in a boiling state, so that starch in the manioc waste becomes pasty, then adding with enzyme, carrying out enzymolysis under ultrasound, deactivating enzyme, centrifuging, and removing the supernate; adding a sodium hydroxide solution into the residue for alkaline hydrolysis, washing, filtering, and drying, and thus obtaining manioc waste cellulose. The invention further discloses a method for preparing a heavy metal adsorption material by adopting the manioc waste cellulose as the raw material, and an adsorption method of heavy metal ions. The manioc waste is taken as a starting material, the cellulose is extracted from the manioc waste through a method combining bio-enzyme and alkaline hydrolysis, the manioc waste cellulose with high content can be rapidly and efficiently obtained, the waste is turned into valuable, the obtained heavy metal adsorption material has great adsorption capacity for the heavy metal ions in water, especially has an excellent adsorption effect for cadmium and lead in the water, and therefore, the manioc waste cellulose has good economy and environment protection value.

The invention discloses a method for extracting grease and protein simultaneously from cereal germs, and pertains to the technical field of comprehensive utilization of agricultural byproducts. The method comprises the comprehensive utilization of technologies of reversed micellar extraction, ultrasonic-associated extraction, protein refining, grease refining, drying and so on. Cereal germs are sieved after crushing, added into reversed micellar solution, stirred or ultrasonic forward extracted and centrifugated to obtain forward extraction solution, and the forward extraction solution is processed by ultrasonic backward extraction and centrifugation, the upper layer is extracted by ethanol to remove a residual surfactant, a solvent is removed to obtain grease and after water, salt and the surfactant are removed from the lower layer, cereal germ protein product is obtained; or the solvent in the forward extraction solution is directly removed, protein in a residual solid substance is precipitated by a mixed solvent, and then washing and drying are carried out to obtain the cereal germ protein product. The method has simple technology and low cost, solves the problem that the traditional grease production causes damage to protein, improves reutilization value of cereal germ protein, and is an environmental friendly production method. The product has the advantages of high yield rate, high purity, low chemical modification and the like.

The invention provides a method for extracting dietary fibers from dendrobium officinale. The method is used for extracting dietary fibers components of parts of stems, leaves and the like of the dendrobium officinale by a flash extraction device. Compared with the prior art, the method is moderate in extraction conditions and short in extraction time; the extraction time is only 1-3 minutes; the dosage of a solvent is small and the extraction efficiency is high; the extraction efficiency is up to 4.16%-5.68%; the separated extraction of water-soluble dietary fibers and water-dissoluble dietary fibers of the dendrobium officinale is realized; the effective extraction efficiency and the comprehensive utilization rate of components of the dendrobium officinale are improved; the wastes of resources are avoided.

The invention discloses a method for extracting effective components in holothurian by means of salting out, belonging to the technical field of the further processing of marine products. The method comprises the following steps of: directly adding some electrolyte and common organic solvent into holothurian extracting liquid to form into an organic solvent / salt multiphase salting out and extracting system with the hydration of salt ion; separating and concentrating the effective components according to the difference of solubility and the different distribution of the different effective components with different polarities in the holothurian in each phase; adding salt and hydrophilic low-molecular organic matter into holothurian extract liquid according to a certain proportion, evenly stirring, stewing or decentralizing to form into double water phase; and adding the salt, the hydrophilic low-molecular organic matter and the hydrophobic organic matter into the holothurian extracting liquid, evenly stirring, stewing or decentralizing to form into a three-liquid phase salting out extracting system. The method develops a novel salting out extracting method which can simultaneously distill and separate various effective components in the holothurian, is simple in operation, moderate in condition and low in cost, and effectively reduces the toxic action of heavy metal.

The invention discloses a mulberry active phenolic compound and a pollution-free extraction and purification method thereof. The method comprises the steps that microwave and vapor high temperature instantaneous enzyme deactivation is carried out on fresh mulberries in sequence, then mechanical crushing, centrifugal layering, food grade acidified alcohol extraction, vacuum concentration and ultrafiltration membrane purification are carried out, and therefore a mulberry phenolic compound extracting solution with the biological activity is obtained. An organic reagent with the low concentration is adopted, mulberry crushing and phenolic compound extraction and purification are achieved under the mild condition, and the natural character and biological activity of the mulberry phenolic compound can be maintained. Compared with a conventional extraction method, the mulberry extracting solution has the higher-concentration total phenol and total anthocyanins, the extraction solution has the good color and luster and high oxidation resistance, alpha-glucosidase enzymatic inhibitory activity and food safety, and the mulberry active phenolic compound can be used as a safety raw material of antioxidant and hypoglycemic drug and health care product development. The conditions are mild, operation is convenient, and industrial production of high-activity natural products is facilitated.

The invention discloses an extraction method of wolfberry polysaccharide, which comprises the following steps of: (1) arranging a wolfberry powder in an extractor, adding petroleum ether into the extractor to carry out extraction, and drying the extract to obtain a degreasing powder; (2) preparing a cellulase solution by using cellulase; (3) dissolving the degreasing powder in the cellulase solution, and carrying out ultrasound extraction; (4) heating the extract to kill enzyme, removing solids by carrying out air pump filtration on the extract after cooling the extract until room temperature to obtain an extracting solution; (5) carrying out evaporation on the extracting solution, and carrying out concentration to obtain a concentrated solution; (6) carrying out alcohol precipitation; and (7) carrying out centrifugation on an alcohol precipitation fluid, taking out a precipitate, washing the precipitate by absolute ethyl alcohol, and carrying out freeze-drying on the precipitate to obtain the wolfberry polysaccharide. With the adoption of the extraction method, the yield of the wolfberry polysaccharide is high, can reach 6.32 percent and is improved by 62.6 percent compared with the traditional hot water extraction method; the extraction time is greatly shortened and is one ninth of time needed by the hot water extraction method, and therefore, the extraction efficiency is improved; and the extraction temperature is lowered, the extraction condition is mild, and the stereochemical structure and the biological activity of the wolfberry polysaccharide can be effectively protected.

The invention relates to a novel rubidium-potassium separating extracting agent 4-methyl-2-(alpha-methylbenzyl) phenol and a rubidium-potassium separating method. 4-methyl-2-(alpha-methylbenzyl) phenol is taken as the extracting agent, and solvent oil such as D60, and the like, is taken as a diluent for extracting and separating rubidium in alkalescent high-potassium brine, wherein single-stage extraction rate of the rubidium can be over 85%, a rubidium-potassium separating coefficient can be over 30; enriched liquor obtained by reversely extracting an organic phase loaded with rubidium through acid liquor can be evaporated and concentrated to prepare rubidium salt products such as RuCl, RuNO3, and the like. Compared with the normal rubidium extracting agent 4-tertiary butyl-2-(alpha-methylbenzyl) phenol, the novel extracting agent 4-methyl-2-(alpha-methylbenzyl) phenol has the advantages of being high in separating efficiency, simple in synthetic method, low in price, mild in extracting condition, and the like. The extracting agent is applicable to underground brine, salt lake brine and solid ore-decomposed rubidium-containing liquor.

A white kidney bean extract extracted by microwave is disclosed, wherein the bioactivity of an alpha-amylase inhibitor contained in the extract is equal to or higher than 4100 U / mg, and the HPLC (high-performance liquid chromatography) content is equal to or higher than 20% w / w. Preparation method for the extract is characterized by comprising the following steps of: crushing white kidney beans and passing through a 60-mesh sieve; preparing a mixture which is uniformly mixed by the crushed and sieved white kidney bean powder and purified water in a weight ratio of 1:5, extracting for 1-10 minutes at a temperature below 40 DEG C in microwave extraction equipment, then filtering to obtain a filtrate, and removing the filter residue; heating the filtrate to 70 DEG C in a water bath with stirring, keeping for 20 minutes, and collecting an effluent through a silica gel column; and drying the effluent to prepare the white kidney bean extract. The extract can be used for preparing an appetite inhibitor. The preparation method disclosed by the invention shortens extraction time and effectively increases extraction rate. The purified water is used as an extraction solvent, as well as is safe and environment-friendly.

The invention discloses a method for extracting and separating curcumin and curcuma oil from carcuma longa, which comprises the following steps of: grinding the carcuma longa into carcuma longa powder with particle size of 80-100 meshes; taking ethanol water solution as extractant for dynamic countercurrent extraction of the carcuma longa powder to obtain extraction solution, and concentrating to obtain concentrated solution; and adding the concentrated solution with plant oil extraction solvent for liquid-liquid extraction to obtain light phase containing curcuma oil and heavy phase containing curcumin. Compared with the prior art, the invention takes the ethanol water solution as the extractant and extracts the curcumin and the curcuma oil from the carcuma longa by employing the dynamic countercurrent extraction method, and the method has the advantages of simple operation, moderate extraction condition, low usage amount of solvent, short extraction time, few impurities in extraction solution and high extraction efficiency.

The invention provides a method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis, comprising the following steps of: grinding walnut raw materials into walnut powder, adding 100,000 to 1000,000 units of protease or composite protease to 1kg of walnut powder, adding water in the mass ratio of the walnut powder to the water is 1: (0.5-20), and then performing microwave processing for 1-10 minutes under the stirring condition of 60 to 300rpm when the microwave power is 10 to 1000 W / L and the temperature is 35 to 65 DEG C, and performing centrifugal separation of oil phase, aqueous phase and solid phase after the reaction ends, wherein the oil phase is walnut oil, the aqueous phase is walnut protein peptide, and the solid phase is residues. The method provided by the invention has mild extraction condition and excellent product performance, and can improve the efficiency of ingress of oil. Furthermore, the obtained walnut oil is higher in quality and is easy to refine; the processing time is greatly shortened by microwave-assisted extraction; the oil phase, the aqueous phase and the solid phase residues can be separated at the same time, therefore, the production efficiency is efficiently improved; and no waste water, waste air or waste residues exists in the producing process, therefore, the invention is environmentally-friendly.

The invention discloses a process method for extracting general flavone of golden camellia leaves by a CO2 supercritical method. The process method comprises the following steps: 1) crushing golden camellia powder to 20-100 meshes and placing the powder in an extraction tank, wherein 55-85% ethanol is taken as a carrying agent and supercritical CO2 is taken as a solvent, thereby obtaining ethanol liquor of general flavone of golden camellia leaves under a condition that the temperature of the extraction tank is 35-55 DEG C, the pressure is 25-35MPa, the CO2 flow is 10-20L / h, the temperature of a first level separation tank is 25 DEG G, the pressure is 6-8MPa, the temperature of a second level separation tank is 25 DEG G, the pressure is 6MPa, and the extracting time is 2-4 hours; and 4) decompressing and distilling to recover ethanol, and cooling and drying to obtain a dark brown extract of general flavone of golden camellia leaves. The general flavone extracted by the method is free from residual organic solvent, and the method is simple, convenient and feasible and high in yield, and the yield can reach 3.81%. The method can be widely applied to the fields such as foods and medicines, and the economical and social benefits of golden camellia can be remarkably improved.

The invention provides a process for extracting chondroitin sulfate from pigs, which comprises: raw material treatment, enzymolysis, absorption decolorizing, salting of protein, alcohol precipitation and crystallization, vacuum drying and the like. In method disclosed by the invention, the extraction rate of chondroitin sulfate is taken as an index, a static optimizing method combining single factor experiment, factorial experiment and response overall analysis experiment is adopted to optimize the extraction process, the process conditions are reasonable, and the effect is very stable. The method has the advantages of extracting the chondroitin sulfate from pig gristles by one step of enzymolysis reaction, ensuring white product color and high product purity, avoiding pollution caused by alkaline extraction, contributing to environment protection, making industrial production easy, along with mild extraction conditions, short production period, and high extraction rate.

The invention relates to the field of jerusalem artichoke processing and utilization and in particular relates to a method for extracting high-purity inulin from jerusalem artichoke by an ultrasonic-complex enzyme method, solving the problem that existing processes for extracting inulin from jerusalem artichoke and processes for purification respectively have various defects. The method comprises an extraction procedure and a purification procedure, wherein the extraction procedure comprises the steps of slicing, drying and grinding tubers of jerusalem artichoke, adding water to dissolve the powder, then adding a complex enzyme and putting the mixture into an ultrasonic washing unit to undergo ultrasonic extraction; carrying out enzyme deactivation and filtration, thus obtaining coarse inulin extract; the purification procedure comprises the steps of forming a dynamic cascade resin chromatographic column with resins D280 and D151; firstly subjecting the coarse inulin extract to the resin D280, then subjecting the coarse inulin extract to the resin D151 and collecting the effluent; subjecting the collected fluid to an ultrafiltration membrane with molecular weight cutoff of 10KDa and a nanofiltration membrane with molecular weight cutoff of 500Da respectively and drying and grinding the cutoff fluid into powder, thus obtaining inulin. The method has the beneficial effects that the inulin yield is greatly increased, the extraction time and temperature are obviously reduced, the inulin yield is high, the product properties and quality are stable and the aims of large-scale production, high yield and good quality are reached.