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Process for extracting chondroitin sulfate from pigs

An extraction process, the technology of chondroitin, applied in the field of extraction process of porcine chondroitin sulfate, can solve the problems of affecting biological activity, long production cycle, unstable quality, etc., and achieve the effects of reduced dosage, short production cycle and easy industrial production

Active Publication Date: 2011-07-27
HUNAN WUXING BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is that the extraction process of pig chondroitin sulfate is mainly alkali extraction at present, the production cycle is long, the extraction rate is low, the color is darker, the quality is unstable, concentrated alkali easily degrades chondroitin sulfate, and affects its biological activity. And the waste material is likely to cause problems such as pollution to the environment, and provide a kind of technology that provides porcine chondroitin sulfate, shorten production cycle, improve the extraction rate and purity of chondroitin sulfate, reduce production cost

Method used

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  • Process for extracting chondroitin sulfate from pigs
  • Process for extracting chondroitin sulfate from pigs
  • Process for extracting chondroitin sulfate from pigs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Pretreatment: remove the fat and meat residue from the fresh pork nose bone, and cut it into small pieces with a length of 2.0 cm;

[0029] (2) Enzymolysis: 5.0 kg of pig nose bone with a moisture content of 82% is put into the reactor, and 20.0 L of distilled water is added, and 0.04 kg of trypsin and alkaline endoprotease are added in a mass ratio of 1: 1. For the complex protease, adjust the pH value to 8.5 with 3.0 mol / L sodium hydroxide solution, control the temperature at 55°C, and mechanically stir for 6 hours to obtain the enzymatic hydrolysis solution;

[0030] (3) Adsorption decolorization: filter the enzymatic solution in step (3) to obtain 22.6 L of filtrate, and adjust the pH value to 6.0 with 4.0 mol / L hydrochloric acid, add 0.11 kg of kaolin and 0.68 kg of activated carbon to stir and adsorb for 1 hour, temperature control At 50°C, filter while hot to obtain 22.0L of adsorption filtrate;

[0031] (4) Protein salting out: adjust the pH value of 22.0L ...

Embodiment 2

[0035] (1) Pretreatment: remove the grease and meat residue from the fresh pig throat and trachea, and cut them into small pieces with a length of 3.0 cm;

[0036] (2) Enzymolysis: Put 8.0 kg of pig laryngeal bone and trachea fragments with a moisture content of 79% into the reactor, add 40.0 L of distilled water, and add 0.08 kg of microbial alkaline protease and alkaline endolytic The protease is compounded at a mass ratio of 1:3, the pH value is adjusted to 8.0 with 1.0 mol / L potassium hydroxide solution, the temperature is controlled at 58° C., and mechanically stirred for 8 hours to obtain an enzymolyzed solution;

[0037] (3) Adsorption and decolorization: Centrifuge the enzymolysis solution in step (2) to remove slag, obtain 45.0 L of supernatant, and adjust the pH value to 4.0 with 6.0 mol / L hydrochloric acid, add 0.68 kg of kaolin and 0.90 kg of activated carbon to stir and adsorb 1.5 hour, the temperature was controlled at 47°C, and 44.5L of adsorption filtrate was o...

Embodiment 3

[0042] (1) Pretreatment: remove the fat and meat residue from the fresh pig soft ribs and crescent bones, and cut them into small pieces with a length of 1.5cm;

[0043] (2) Enzymolysis: Put 10.0 kg of pig soft rib and crescent bone with a moisture content of 75% into the reactor, add 30.0 L of distilled water, add 0.09 kg of trypsin and microbial alkaline protease in a mass ratio of 1 : 3 complex compound proteases, the pH value is adjusted to 8.7 with 2.0mol / L potassium hydroxide solution, the temperature is controlled at 60°C, and mechanically stirred for 12 hours to obtain the enzymolysis solution;

[0044] (3) Adsorption decolorization: filter the enzymolysis solution in step (2) to obtain 35.8 L of filtrate, adjust the pH value to 5.0 with 2.0 mol / L sulfuric acid, add 0.72 kg of kaolin and 0.54 kg of activated carbon to stir and adsorb for 2 hours, temperature control At 45°C, filter while hot to obtain 35.2L of adsorption filtrate;

[0045] (4) Protein salting out: the...

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Abstract

The invention provides a process for extracting chondroitin sulfate from pigs, which comprises: raw material treatment, enzymolysis, absorption decolorizing, salting of protein, alcohol precipitation and crystallization, vacuum drying and the like. In method disclosed by the invention, the extraction rate of chondroitin sulfate is taken as an index, a static optimizing method combining single factor experiment, factorial experiment and response overall analysis experiment is adopted to optimize the extraction process, the process conditions are reasonable, and the effect is very stable. The method has the advantages of extracting the chondroitin sulfate from pig gristles by one step of enzymolysis reaction, ensuring white product color and high product purity, avoiding pollution caused by alkaline extraction, contributing to environment protection, making industrial production easy, along with mild extraction conditions, short production period, and high extraction rate.

Description

technical field [0001] The invention relates to a process for extracting porcine chondroitin sulfate, in particular to a method for extracting chondroitin sulfate from pig cartilage by using a complex protease, and belongs to the field of bioengineering. Background technique [0002] Chondroitin Sulfate (CS) is an important acidic mucopolysaccharide derived from animal cartilage tissue, belonging to glycosaminoglycans, mainly distributed in animal cartilage, laryngeal bone, nasal bone, cattle, In the trachea, it is also contained in other tissues such as leg bones, ligaments, skin, and cornea. Chondroitin sulfate is mainly composed of D-glucuronic acid and N-acetyl-D-galactosamine connected by β-1,3 glycosidic bonds to form disaccharides, and the disaccharide units are connected by β-1,4 glycosidic bonds to form biological macromolecule. According to the position of the sulfate group in the structure, chondroitin sulfate can be divided into A, C, D, E, K, M, L and other is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C12S3/02
Inventor 杨文革胡永红陈召国
Owner HUNAN WUXING BIOLOGICAL TECH CO LTD
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