Technology for preparing functional dietary fiber through waste bamboo shell enzymolysis
A dietary fiber and functional technology, applied in food preparation, food science, application, etc., can solve problems such as rot and mildew, ecological environment pollution in bamboo shoot production areas, and odor generation, achieve high yield, solve environmental pollution and resource The effect of wasting and controlling cholesterol
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Embodiment 1
[0015] (1) Preparation of bamboo shoot shells: recover fresh bamboo shoot shells, select, wash, pulverize (homogenize) or dry at 60-70°C for later use.
[0016] (2) Organic solvent extraction: Weigh 250g of the dry powder slurry (the slurry is dried at 60-70°C, crushed, and the weight of the dry powder passed through a 80-100 mesh sieve), put it in a closed container, and use the organic solvent diethyl ether to seal and extract the slurry. Lipids in bamboo shoot shells are removed, and the amount of organic solvent ether added is based on submerging the bamboo shoot shells. After leaching for 24 hours, the organic solvent is recovered by pressure filtration, and air-dried or oven-dried to remove residual organic solvents in the bamboo shoot shells.
[0017] (3) Vitrinase enzymatic hydrolysis: in the degreased bamboo shoot shell residue, add phosphate buffer solution 17mL / g (bamboo shoot shell dry matter weight) of pH=5 and enzyme concentration of 1%, enzyme activity is 6u / mL ...
Embodiment 2
[0026] (1) Preparation of bamboo shoot shells: recover fresh bamboo shoot shells, dry and crush them at 60°C, pass through 80-mesh sieves, and set aside.
[0027] (2) Organic solvent extraction: Weigh 300g of dry powder slurry, put it in a closed container, seal and extract with organic solvent ether, and remove the lipids in the bamboo shoot shells. The amount of organic solvent ether added is based on submerged bamboo shoot shells. After extracting for 24 hours, the organic solvent is recovered by pressure filtration, air-dried or oven-dried to remove the residual organic solvent in the bamboo shoot shell.
[0028] (3) Vitrinase enzymatic hydrolysis: in the degreased bamboo shoot shell residue, add 20 mL / g of phosphate buffer solution (dry weight of bamboo shoot shell) with pH=4.5 and enzyme concentration of 0.5%, enzyme activity of 8u / mL Dextran enzyme solution, at 120rpm, in a 60°C water bath, hydrolyze for 2 hours, raise the temperature to 100°C, and keep for 5 minutes to...
Embodiment 3
[0036] (1) Bamboo shoot shell preparation: recycle the discarded hairy shoot shells from bamboo shoot processing, dry and pulverize at 60°C, pass through an 80-mesh sieve, and set aside.
[0037] (2) Organic solvent extraction: Weigh 500g of dry powder slurry, put it in a closed container, seal and extract with organic solvent ether, and remove the lipids in the bamboo shoot shells. The amount of organic solvent ether added is based on submerged bamboo shoot shells, After extracting for 24 hours, press filter, dry the bamboo shoot shell, remove the residual organic solvent in the bamboo shoot shell, and recover the organic solvent.
[0038] (3) Vitrinase enzymatic hydrolysis: in the degreased bamboo shoot shell residue, add 20mL / g of phosphate buffer solution (dry weight of bamboo shoot shell) with pH=4.5 and 1% enzyme concentration, enzyme activity is 6u / mL Cellulase solution, at 120rpm, in a 50°C water bath, for 1 hour of enzymatic hydrolysis.
[0039] (4) Decolorization an...
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