Method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis
A technology of protease hydrolysis and walnut protein, which is applied in the field of preparation of walnut oil and walnut protein peptide, and microwave-assisted protease hydrolysis to simultaneously prepare walnut oil and walnut protein peptide, which can solve the problem of complex walnut oil and walnut protein peptide process and low quality of walnut oil problem, achieve the effect of shortening processing time, increasing diversity and improving production efficiency
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Embodiment 1
[0026] A. Grind uncoated walnut kernels into walnut puree;
[0027] B. Take 1kg of the walnut puree of step A, and add 100,000 units of papain to it, and then add 0.5kg of purified water to mix;
[0028] C. Under the stirring condition of 60rpm, the microwave power is 1000W / L, and the temperature is 65°C, microwave the mixture in step B for 3 minutes, after the reaction is over, centrifuge to separate oil, water, and solid phase, wherein: separated to obtain The 0.5kg oil phase is walnut oil, the 0.35kg water phase is protein peptide aqueous solution, which is walnut protein peptide, and the 0.65kg solid phase is residue.
[0029] The oil phase obtained in this example is clear walnut oil, and the water phase is boiled at 90°C for 3 minutes to inactivate the enzyme, then freeze-dried to obtain 0.2 kg of walnut protein peptide coarse powder, and the solid phase residue is dried and crushed to obtain dietary fiber food 0.3kg.
Embodiment 2
[0031] A. Grind uncoated walnut kernels into walnut puree;
[0032] B. Get the walnut puree of 1kg step A, and add the compound protease that is formed by the pineapple enzyme of 500,000 units and the papain of 500,000 units, then add 1kg of deionized water and mix;
[0033] C. Under the stirring condition of 120rpm, the microwave power is 600W / L, and the temperature is 45°C, microwave the mixture of step B for 10 minutes, after the reaction, centrifuge to separate oil, water and solid phase, of which: 0.52kg The oil phase is walnut oil, the 0.83kg water phase is an aqueous protein peptide solution, which is walnut protein peptide, and the 0.65kg solid phase is residue.
[0034] The oil phase obtained in this example is clear walnut oil, and the water phase is heated at 90°C for 5 minutes for enzyme inactivation treatment, and then spray-dried to obtain 0.22 kg of walnut protein peptide coarse powder, and the solid phase residue is dried and pulverized to obtain dietary fib...
Embodiment 3
[0036] A. Grinding undeoiled walnut coarse powder into walnut puree;
[0037] B. Get the walnut puree of 1kg step A, and add the compound protease that is made up of 10,000 units of pepsin and 500,000 units of pineapple enzyme, then add 20kg of distilled water and mix;
[0038] C. Under the stirring condition of 300rpm, microwave the mixture in Step B for 1 minute at a microwave power of 10W / L and a temperature of 35°C. After the reaction, centrifuge to separate oil, water, and solid phase, of which: 0.54kg The oil phase is walnut oil, the 19.8kg water phase is a protein peptide aqueous solution, which is walnut protein peptide, and the 0.66kg solid phase is residue.
[0039] The oil phase obtained in this example is clear walnut oil, and the water phase is boiled at 90°C for 3 minutes to inactivate the enzyme, and then intercepted by a membrane filter to obtain 0.45 kg of a component solution with a molecular weight between 200 and 8000 Daltons , 0.45kg of components grea...
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