Method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis

A technology of protease hydrolysis and walnut protein, which is applied in the field of preparation of walnut oil and walnut protein peptide, and microwave-assisted protease hydrolysis to simultaneously prepare walnut oil and walnut protein peptide, which can solve the problem of complex walnut oil and walnut protein peptide process and low quality of walnut oil problem, achieve the effect of shortening processing time, increasing diversity and improving production efficiency

Inactive Publication Date: 2011-06-01
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the problems that the preparation process of walnut oil and walnut protein peptide is complicated and the quality of the obtained walnut oil is not high, the present invention provides a method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis, which can efficiently and quickly obtain walnut oil under relatively mild conditions Oil, walnut protein peptide, walnut dietary fiber products, making its production process cost-effective and environmentally friendly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A. Grind uncoated walnut kernels into walnut puree;

[0027] B. Take 1kg of the walnut puree of step A, and add 100,000 units of papain to it, and then add 0.5kg of purified water to mix;

[0028] C. Under the stirring condition of 60rpm, the microwave power is 1000W / L, and the temperature is 65°C, microwave the mixture in step B for 3 minutes, after the reaction is over, centrifuge to separate oil, water, and solid phase, wherein: separated to obtain The 0.5kg oil phase is walnut oil, the 0.35kg water phase is protein peptide aqueous solution, which is walnut protein peptide, and the 0.65kg solid phase is residue.

[0029] The oil phase obtained in this example is clear walnut oil, and the water phase is boiled at 90°C for 3 minutes to inactivate the enzyme, then freeze-dried to obtain 0.2 kg of walnut protein peptide coarse powder, and the solid phase residue is dried and crushed to obtain dietary fiber food 0.3kg.

Embodiment 2

[0031] A. Grind uncoated walnut kernels into walnut puree;

[0032] B. Get the walnut puree of 1kg step A, and add the compound protease that is formed by the pineapple enzyme of 500,000 units and the papain of 500,000 units, then add 1kg of deionized water and mix;

[0033] C. Under the stirring condition of 120rpm, the microwave power is 600W / L, and the temperature is 45°C, microwave the mixture of step B for 10 minutes, after the reaction, centrifuge to separate oil, water and solid phase, of which: 0.52kg The oil phase is walnut oil, the 0.83kg water phase is an aqueous protein peptide solution, which is walnut protein peptide, and the 0.65kg solid phase is residue.

[0034] The oil phase obtained in this example is clear walnut oil, and the water phase is heated at 90°C for 5 minutes for enzyme inactivation treatment, and then spray-dried to obtain 0.22 kg of walnut protein peptide coarse powder, and the solid phase residue is dried and pulverized to obtain dietary fib...

Embodiment 3

[0036] A. Grinding undeoiled walnut coarse powder into walnut puree;

[0037] B. Get the walnut puree of 1kg step A, and add the compound protease that is made up of 10,000 units of pepsin and 500,000 units of pineapple enzyme, then add 20kg of distilled water and mix;

[0038] C. Under the stirring condition of 300rpm, microwave the mixture in Step B for 1 minute at a microwave power of 10W / L and a temperature of 35°C. After the reaction, centrifuge to separate oil, water, and solid phase, of which: 0.54kg The oil phase is walnut oil, the 19.8kg water phase is a protein peptide aqueous solution, which is walnut protein peptide, and the 0.66kg solid phase is residue.

[0039] The oil phase obtained in this example is clear walnut oil, and the water phase is boiled at 90°C for 3 minutes to inactivate the enzyme, and then intercepted by a membrane filter to obtain 0.45 kg of a component solution with a molecular weight between 200 and 8000 Daltons , 0.45kg of components grea...

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PUM

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Abstract

The invention provides a method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis, comprising the following steps of: grinding walnut raw materials into walnut powder, adding 100,000 to 1000,000 units of protease or composite protease to 1kg of walnut powder, adding water in the mass ratio of the walnut powder to the water is 1: (0.5-20), and then performing microwave processing for 1-10 minutes under the stirring condition of 60 to 300rpm when the microwave power is 10 to 1000 W / L and the temperature is 35 to 65 DEG C, and performing centrifugal separation of oil phase, aqueous phase and solid phase after the reaction ends, wherein the oil phase is walnut oil, the aqueous phase is walnut protein peptide, and the solid phase is residues. The method provided by the invention has mild extraction condition and excellent product performance, and can improve the efficiency of ingress of oil. Furthermore, the obtained walnut oil is higher in quality and is easy to refine; the processing time is greatly shortened by microwave-assisted extraction; the oil phase, the aqueous phase and the solid phase residues can be separated at the same time, therefore, the production efficiency is efficiently improved; and no waste water, waste air or waste residues exists in the producing process, therefore, the invention is environmentally-friendly.

Description

technical field [0001] The invention relates to a method for preparing walnut oil and walnut protein peptide, in particular to a method for synchronously preparing walnut oil and walnut protein peptide through microwave-assisted protease hydrolysis, belonging to the technical field of food or health food. Background technique [0002] Walnut( Juglans regia L .) Also known as walnuts and Qiang peaches, it belongs to the Juglandaceae Juglans plant. Walnut kernels contain up to 65% oil and more than 15.4% protein, as well as phospholipids, multivitamins, and linoleic acid and linolenic acid in sufficient quantities and proportions. Walnut oil has high medical and edible value, and its preparation methods include pressing method, water belt method, solvent extraction method and so on. In recent years, walnut oil has been extracted by water enzymatic method, and the yield of clear oil can reach 40%. Juglans mandshurca Maxlm ) to extract hickory nut oil by water enzymatic meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12P21/02
Inventor 陈朝银赵声兰葛锋韩本勇刘迪秋熊向峰
Owner KUNMING UNIV OF SCI & TECH
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