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Method for extracting and separating ganoderma lucidum polysaccharide from ganoderma lucidum sporocarp

A technology of Ganoderma lucidum fruiting bodies and Ganoderma polysaccharides, which is applied in the field of extraction and separation of Ganoderma lucidum polysaccharides, can solve problems such as difficulties in large-scale production, achieve the effects of reducing solvent consumption, ensuring activity, and simple equipment

Active Publication Date: 2011-10-19
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the separation and purification process of Ganoderma lucidum polysaccharides, using ion resin to treat Ganoderma lucidum crude polysaccharides can effectively separate and purify, but because the ion column is an axial column, it has relatively great difficulties in large-scale production

Method used

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  • Method for extracting and separating ganoderma lucidum polysaccharide from ganoderma lucidum sporocarp
  • Method for extracting and separating ganoderma lucidum polysaccharide from ganoderma lucidum sporocarp
  • Method for extracting and separating ganoderma lucidum polysaccharide from ganoderma lucidum sporocarp

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Compound enzymatic hydrolysis to extract polysaccharides from Ganoderma lucidum fruiting bodies: select the crushed Ganoderma lucidum fruiting bodies that pass through a 100-mesh sieve, take 50.0 g of Ganoderma lucidum fruiting body powder, add it to 1000 g of water, and adjust the pH of the solution to 6.8 with saturated calcium hydroxide solution, Add 1.5g compound enzyme, the compound enzyme is papain (Shanghai Lanji Technology Development Co., Ltd., 1 million U / g) and cellulase (Zhangjiagang Jinyuan Biochemical Co., Ltd., 80,000 U / g) mass ratio 2:1 The mixture was placed in a constant temperature water bath at 55°C, stirred at a constant speed for 2 hours, centrifuged at 4000rpm for 10 minutes, and the supernatant was taken to obtain the enzymatic hydrolysis solution;

[0032] (2) Tangential flow ultrafiltration membrane concentrated enzymatic hydrolysis solution: the enzymatic hydrolysis solution enters the tangential flow filtration system (Congent M, Millipore...

Embodiment 2

[0040] (1) Compound enzymatic hydrolysis to extract polysaccharides from Ganoderma lucidum fruiting body: select the crushed Ganoderma lucidum fruiting body that has passed through a 100-mesh sieve, take 50.0 g of Ganoderma lucidum fruiting body powder, add it to 500 g of water, and adjust the pH of the solution to 6.0 with saturated calcium hydroxide solution. Add 1.2g compound enzyme, the compound enzyme is papain (Shanghai Lanji Technology Development Co., Ltd., 1 million U / g) and cellulase (Zhangjiagang Jinyuan Biochemical Co., Ltd., 80,000 U / g) mass ratio 1:1 The mixture was placed in a constant temperature water bath at 40°C, stirred at a constant speed for 1 hour, centrifuged at 4000rpm for 12 minutes, and the supernatant was taken to obtain the enzymatic hydrolysis solution;

[0041] (2) Tangential flow ultrafiltration membrane concentrated enzymolysis solution: the enzymolysis solution enters the tangential flow filtration system, selects an ultrafiltration membrane wi...

Embodiment 3

[0046] (1) Complex enzymatic hydrolysis to extract polysaccharides from Ganoderma lucidum fruiting bodies: select the crushed Ganoderma lucidum fruiting bodies that pass through a 100-mesh sieve, take 50.0 g of Ganoderma lucidum fruiting body powder, add it to 1500 g of water, and adjust the pH of the solution to 4.5 with a saturated calcium hydroxide solution. Add 0.5g compound enzyme, compound enzyme is papain (Shanghai Lanji Technology Development Co., Ltd., 1,000,000 U / g) and cellulase (Zhangjiagang Jinyuan Biochemical Co., Ltd., 80,000 U / g) mass ratio 4:1 The mixture was placed in a constant temperature water bath at 65°C, stirred at a constant speed for 3 hours, centrifuged at 4000rpm for 12 minutes, and the supernatant was taken to obtain the enzymatic hydrolysis solution;

[0047] (2) Tangential flow ultrafiltration membrane concentrated enzymolysis solution: the enzymolysis solution enters the tangential flow filtration system, selects an ultrafiltration membrane with ...

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Abstract

The invention discloses a method for extracting and separating ganoderma lucidum polysaccharide from ganoderma lucidum sporocarp. The method comprises the steps of carrying out enzymolysis and extraction with complex enzyme on the ganoderma lucidum sporocarp to obtain enzymolysis solution, concentrating and separating the enzymolysis solution through a tangential flow filtering membrane to obtainultrafiltration retention solution, carrying out elution, separation and purification on the ultrafiltration retention solution with purified water through radial flow chromatograph to obtain polysaccharide eluent, and conducting reduced pressure concentration and drying on the polysaccharide eluent to obtain ganoderma lucidum polysaccharide, wherein the papain and and cellulose are mixed in a weight ratio of 1:2-4:1 to form complex enzyme. The invention builds a set of polysaccharide production process of ganoderma lucidum sporocarp from raw materials to products, the process is environment-friendly, fast and efficient and suitable for large-scale production and the process cost for refining ganoderma lucidum polysaccharide can be greatly reduced.

Description

technical field [0001] The invention relates to a method for extracting and separating ganoderma polysaccharides from ganoderma lucidum fruiting bodies. Background technique [0002] Modern pharmacology and clinical practice have proved that Ganoderma lucidum polysaccharides are the main components of Ganoderma lucidum to strengthen the body, nourish and strengthen, and prolong life. Ganoderma lucidum polysaccharide has the characteristics of stimulating the host's non-specific resistance, immune specific reaction and inhibiting the physiological activity of transplanted tumors. It can improve the body's immunity and hypoxia resistance, and the market demand is increasing. [0003] The traditional extraction method of Ganoderma lucidum polysaccharide is: Ganoderma lucidum fruiting body crushing-water extraction / acid-base extraction-alcohol precipitation-drying, which is easy for large-scale production, but the obtained polysaccharide has many impurities, low content of activ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 邵平姜慧燕林炳谊孙培龙
Owner ZHEJIANG UNIV OF TECH
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