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Method for extracting blueberry seed oil by adopting ultrasonic-assisted aqueous enzymatic method

An ultrasonic-assisted, blueberry seed technology is applied in the direction of fat oil/fat production and fat production, etc. It can solve the problems of dark blueberry seed oil and crude oil, affect the aroma of blueberry seed oil, and is not suitable for industrial production, etc., and achieve high quality crude oil , Save the amount of enzyme used, reduce the effect of adverse effects

Active Publication Date: 2014-07-02
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, there are very few reports on the extraction of blueberry seed oil, and there is an ultrasonic-assisted solvent extraction method (Chen Liang et al., Research on components of blueberry seed oil, China Oils, 2013, 38:10, 84-86.), this method is to use blueberry The extraction of blueberry seed oil based on the solubility of the seed oil in different solvents is relatively simple, and the oil yield of the blueberry seed oil is about 18%, but it needs to repeatedly extract the blueberry seed oil for a long time. The dark color of crude seed oil and many residual solvents affect the aroma of blueberry seed oil to a certain extent, and the solvent volatilizes during the heating process, causing environmental pollution; in addition, there are related reports on the extraction of blueberry seed oil by supercritical extraction (Qu Xiaoyuan et al. Analysis of volatile components of blueberry seed oil, Guizhou Agricultural Sciences, 2013, 41:10, 64-66), this method is to control the solubility and vapor partial pressure of blueberry seed oil in it by adjusting the pressure and temperature of CO2 To extract blueberry seed oil, no organic solvent is needed, but its oil yield to blueberry seed oil is only about 8%, and the required equipment is expensive, so it is not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The blueberry seeds (with a water content of 8%) are crushed into blueberry seed powder with a particle size of 60 mesh. Weigh 10g of crushed blueberry seed powder, and prepare blueberry seed powder suspension with drinking water, the mass ratio of blueberry seed powder to water is 1:3, the water bath temperature is set at 40°C, ultrasonic treatment is 15min, and the ultrasonic power is 400W , adding 1.25% blueberry seed powder protease (purchased from Nanjing Audofoni Biotechnology Co., Ltd.) and blueberry seed powder 1.25% cellulose-pectin compound enzyme (purchased from Nanjing Audofoni Biotechnology Co., Ltd. Company), after stirring evenly, reduce the ultrasonic power to 90W, ultrasonically assist enzymolysis for 30min, then increase the temperature of the water bath to 100°C, inactivate the enzyme in the boiling water bath for 10mim, use a high-speed refrigerated centrifuge, centrifuge at 10000r / min for 25min, and collect The supernatant was obtained as translucen...

Embodiment 2

[0035]The obtained blueberry seeds (with a water content of 9%) were pulverized into blueberry seed powder with a particle size of 60 mesh. Weigh 50g of crushed blueberry seed powder, and prepare blueberry seed powder suspension with drinking water, the mass ratio of blueberry seed powder to water is 1:6, the water bath temperature is set at 60°C, ultrasonic treatment is 20min, and the ultrasonic power is 300W , add 0.5% protease of blueberry seed powder and cellulose-pectin compound enzyme of 1% blueberry seed powder in turn, stir evenly, reduce the ultrasonic power to 90W, ultrasonically assist enzymatic hydrolysis for 20min, and then increase the temperature of the water bath to 100°C, After treating in a boiling water bath for 10 minutes to inactivate the enzyme, use a high-speed refrigerated centrifuge to centrifuge for 30 minutes at a speed of 10,000 r / min, collect the supernatant, and obtain translucent yellow blueberry seed oil. The oil yield of blueberry seeds by this...

Embodiment 3

[0037] The blueberry seeds (with a water content of 10%) are crushed into blueberry seed powder with a particle size of 80 mesh. Weigh 100g of crushed blueberry seed powder, and prepare blueberry seed powder suspension with drinking water, the mass ratio of blueberry seed powder to water is 1:8, the water bath insulation is set at 50°C, ultrasonic treatment is 10min, and the ultrasonic power is 500W , add 0.8% protease of blueberry seed powder, cellulose-pectin compound enzyme of 2.4% blueberry seed powder in turn, stir evenly, reduce the ultrasonic power to 75W, ultrasonically assisted enzymolysis for 40min, and then increase the water bath temperature to 100°C, Treat in a boiling water bath for 10 minutes to inactivate the enzyme, use a high-speed refrigerated centrifuge to centrifuge for 20 minutes at a speed of 10,000 r / min, collect the supernatant, and obtain translucent yellow blueberry seed oil. The oil yield of blueberry seeds by this method was 26.18%, and the unsatur...

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Abstract

The invention discloses a method for extracting blueberry seed oil by adopting an ultrasonic-assisted aqueous enzymatic method. The method for extracting the blueberry seed oil by adopting the ultrasonic-assisted aqueous enzymatic method comprises the following steps: carrying out breaking, pulverization and then enzymolysis treatment on blueberry seed taken as raw material, applying certain ultrasonic for assisting extraction in different stages, then carrying out enzyme deactivation and centrifugation, and taking a supernate, so that the blueberry seed oil is obtained. The method for extracting the blueberry seed oil is simple in operation, mild in conditions, high in oil yield, pollution-free, easy for refining, low in production cost and applicable to large-scale production.

Description

technical field [0001] The invention relates to a method for preparing edible oil, in particular to a method for preparing blueberry seed oil by an ultrasonic-assisted aqueous enzymatic method. Background technique [0002] Blueberry, also known as lingonberry and blueberry, is a plant of the genus Vaccinium in the family Rhododendronaceae, which is a perennial deciduous or evergreen shrub. Blueberry fruit is a berry, nearly round, blue in color, moderately sweet and sour, with delicate flesh, thin skin and small seeds. Blueberries are rich in nutrients, including anthocyanins, vitamin A, vitamin E, ursolic acid, ellagic acid and polyphenols, which can improve vision, anti-aging, enhance heart function, treat cardiovascular diseases and lower cholesterol and other effects. Therefore, blueberries have been popular at home and abroad in recent years, and have been listed as the top of the "15 healthy foods" and one of the "five human health foods" by the British authoritativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04
Inventor 黄开红张宏志马艳弘赵延存李亚辉
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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