Application of extracting agent and deep-eutectic solvent in determining of effective ingredients in traditional Chinese medicine

A technology of active ingredients and deep eutectic solvent, which is applied in the field of determination of flavonoids in Scutellaria baicalensis, can solve the problems of flammability and explosion, and achieve the effect of simple operation, mild extraction conditions and high extraction efficiency

Inactive Publication Date: 2018-05-29
SHENYANG PHARMA UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to address the problems existing in the prior art, and to provide a method for utilizing a deep eutectic solvent to extract active ingredients in traditional Chinese medicines, especially flavonoids in Scutellaria baicalensis. Extracting flavonoids using the present invention can effectively improve the flavonoids The extraction efficiency of compound, and solve the existing problems of using flammable and explosive organic solvents in the flavonoid extraction of the prior art

Method used

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  • Application of extracting agent and deep-eutectic solvent in determining of effective ingredients in traditional Chinese medicine
  • Application of extracting agent and deep-eutectic solvent in determining of effective ingredients in traditional Chinese medicine
  • Application of extracting agent and deep-eutectic solvent in determining of effective ingredients in traditional Chinese medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] 1) Weigh a certain amount of standard products of five flavonoid glycosides, scutellarin, baicalin, wogonin, baicalein and wogonin, and add methanol to make scutellarin, baicalin, wogonin, and baicalin A mixed solution of five flavonoids including wogonin and wogonin;

[0068] 2) Detect 5 kinds of flavonoid components by high performance liquid chromatography ultraviolet detector

[0069] Liquid phase conditions: the organic phase is for detection, the aqueous phase is 0.5% phosphoric acid aqueous solution, and a gradient elution procedure is adopted, the gradient program is shown in Table 1; the flow rate is 1mL min -1 , the column temperature is 25°C, the detection wavelength is 278nm, and 10 μL of sample is injected for detection. The chromatogram is shown in figure 1 .

[0070] Table 1. Gradient elution program

[0071]

Embodiment 2

[0073] 1) Preparation of deep eutectic solvent: choline chloride and 1,2-propanediol with a molar ratio of 1:4 were stirred under anhydrous conditions, heated at 50°C until a uniform and stable transparent solution was formed, and sealed and stored.

[0074] 2) Take the deep eutectic solvent obtained in step (1), add water respectively to obtain a 75% deep eutectic solvent extractant, weigh 100 mg of Scutellaria baicalensis powder, weigh it accurately, add 2.0 mL of the 75% deep eutectic solvent extractant , vortex for 3 minutes, ultrasonic for 30 minutes, centrifuge at 3500rpm for 25 minutes, precisely pipette 1.0 mL of the supernatant into a 10 mL volumetric flask, add distilled water to dilute to the mark, vortex for 2 minutes, and filter through a 0.22 μm membrane to obtain the sample solution.

[0075] 3) Inject 10 μL of the obtained extract into a high-performance liquid chromatograph for detection, and use the detection conditions in Example 1 for detection. The content...

Embodiment 3

[0078] 1) Preparation of deep eutectic solvent: choline chloride and ethylene glycol with a molar ratio of 1:4 were stirred under anhydrous conditions, heated at 50°C until a uniform and stable transparent solution was formed, and sealed and stored.

[0079] 2) Take the deep eutectic solvent obtained in step (1), add water respectively to obtain a 75% deep eutectic solvent extractant, weigh 100 mg of Scutellaria baicalensis powder, weigh it accurately, add 2.0 mL of the 75% deep eutectic solvent extractant , vortex for 3 minutes, ultrasonic for 30 minutes, centrifuge at 3500rpm for 25 minutes, precisely pipette 1.0 mL of the supernatant into a 10 mL volumetric flask, add distilled water to dilute to the mark, vortex for 2 minutes, and filter through a 0.22 μm membrane to obtain the sample solution.

[0080] 3) Inject 10 μL of the obtained extract into a high-performance liquid chromatograph for detection, and use the detection conditions in Example 1 for detection. The content...

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Abstract

The invention relates to the technical field of preparation of extracting agents and deep-eutectic solvents, and relates to application of an extracting agent and a deep-eutectic solvent in determining of effective ingredients in traditional Chinese medicine, in particular to application in determining of flavonoid ingredient in radix scutellariae, and application of an extracting agent and a deep-eutectic solvent in determining of effective ingredients of baicalin, baicalein, wogonoside, wogonin and scutellarin in radix scutellariae. The application is characterized in that the radix scutellariae is used as the raw material, the deep-eutectic solvent, especially choline deep-eutectic solvent, is used as the extracting agent, the water is used as a thinner for reducing the system viscosity, and the flavonoid compound is forcibly extracted under the ultrasonic condition. An extracting method has the advantages that the extracting efficiency of the flavonoid ingredient is higher, the extracting conditions are moderate, and the operation is simple and convenient. The extracting agent has the characteristics of no burning explosion and volatizing, small loss, low energy consumption andthe like. The extracting method is also suitable for extracting the flavonoid-containing traditional Chinese medicines, such as radix astragali seu hedysari, liquorice root, radix puerariae and radixsophorae flavescentis.

Description

【Technical field】 [0001] The present invention relates to the technical field of extraction agent-deep eutectic solvent preparation, and relates to the application of extraction agent-deep eutectic solvent in the determination of active ingredients of traditional Chinese medicine, especially the application in the determination of flavonoids in Scutellaria baicalensis, specifically to the extraction agent-deep eutectic solvent. Application of melting solvent in the determination of effective components of baicalin, baicalein, wogonin, wogonin and scutellarin in Scutellaria baicalensis. 【Background technique】 [0002] Deep eutectic solvent (DES) is a new type of green solvent that is rapidly emerging to replace traditional solvents and ionic liquids. It is a homogeneous liquid formed by hydrogen bonding of two or more components, with low melting point and low cost. , low toxicity, easy preparation, renewable, degradable and other advantages, it has been applied in many field...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/14
CPCG01N30/06G01N30/14G01N2030/062G01N2030/146
Inventor 赵龙山熊志立王漫漫秦峰
Owner SHENYANG PHARMA UNIVERSITY
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