Application of extracting agent and deep-eutectic solvent in determining of effective ingredients in traditional Chinese medicine
A technology of active ingredients and deep eutectic solvent, which is applied in the field of determination of flavonoids in Scutellaria baicalensis, can solve the problems of flammability and explosion, and achieve the effect of simple operation, mild extraction conditions and high extraction efficiency
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Embodiment 1
[0067] 1) Weigh a certain amount of standard products of five flavonoid glycosides, scutellarin, baicalin, wogonin, baicalein and wogonin, and add methanol to make scutellarin, baicalin, wogonin, and baicalin A mixed solution of five flavonoids including wogonin and wogonin;
[0068] 2) Detect 5 kinds of flavonoid components by high performance liquid chromatography ultraviolet detector
[0069] Liquid phase conditions: the organic phase is for detection, the aqueous phase is 0.5% phosphoric acid aqueous solution, and a gradient elution procedure is adopted, the gradient program is shown in Table 1; the flow rate is 1mL min -1 , the column temperature is 25°C, the detection wavelength is 278nm, and 10 μL of sample is injected for detection. The chromatogram is shown in figure 1 .
[0070] Table 1. Gradient elution program
[0071]
Embodiment 2
[0073] 1) Preparation of deep eutectic solvent: choline chloride and 1,2-propanediol with a molar ratio of 1:4 were stirred under anhydrous conditions, heated at 50°C until a uniform and stable transparent solution was formed, and sealed and stored.
[0074] 2) Take the deep eutectic solvent obtained in step (1), add water respectively to obtain a 75% deep eutectic solvent extractant, weigh 100 mg of Scutellaria baicalensis powder, weigh it accurately, add 2.0 mL of the 75% deep eutectic solvent extractant , vortex for 3 minutes, ultrasonic for 30 minutes, centrifuge at 3500rpm for 25 minutes, precisely pipette 1.0 mL of the supernatant into a 10 mL volumetric flask, add distilled water to dilute to the mark, vortex for 2 minutes, and filter through a 0.22 μm membrane to obtain the sample solution.
[0075] 3) Inject 10 μL of the obtained extract into a high-performance liquid chromatograph for detection, and use the detection conditions in Example 1 for detection. The content...
Embodiment 3
[0078] 1) Preparation of deep eutectic solvent: choline chloride and ethylene glycol with a molar ratio of 1:4 were stirred under anhydrous conditions, heated at 50°C until a uniform and stable transparent solution was formed, and sealed and stored.
[0079] 2) Take the deep eutectic solvent obtained in step (1), add water respectively to obtain a 75% deep eutectic solvent extractant, weigh 100 mg of Scutellaria baicalensis powder, weigh it accurately, add 2.0 mL of the 75% deep eutectic solvent extractant , vortex for 3 minutes, ultrasonic for 30 minutes, centrifuge at 3500rpm for 25 minutes, precisely pipette 1.0 mL of the supernatant into a 10 mL volumetric flask, add distilled water to dilute to the mark, vortex for 2 minutes, and filter through a 0.22 μm membrane to obtain the sample solution.
[0080] 3) Inject 10 μL of the obtained extract into a high-performance liquid chromatograph for detection, and use the detection conditions in Example 1 for detection. The content...
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