Extraction method of wolfberry polysaccharide

Abstract

The invention discloses an extraction method of wolfberry polysaccharide, which comprises the following steps of: (1) arranging a wolfberry powder in an extractor, adding petroleum ether into the extractor to carry out extraction, and drying the extract to obtain a degreasing powder; (2) preparing a cellulase solution by using cellulase; (3) dissolving the degreasing powder in the cellulase solution, and carrying out ultrasound extraction; (4) heating the extract to kill enzyme, removing solids by carrying out air pump filtration on the extract after cooling the extract until room temperature to obtain an extracting solution; (5) carrying out evaporation on the extracting solution, and carrying out concentration to obtain a concentrated solution; (6) carrying out alcohol precipitation; and (7) carrying out centrifugation on an alcohol precipitation fluid, taking out a precipitate, washing the precipitate by absolute ethyl alcohol, and carrying out freeze-drying on the precipitate to obtain the wolfberry polysaccharide. With the adoption of the extraction method, the yield of the wolfberry polysaccharide is high, can reach 6.32 percent and is improved by 62.6 percent compared with the traditional hot water extraction method; the extraction time is greatly shortened and is one ninth of time needed by the hot water extraction method, and therefore, the extraction efficiency is improved; and the extraction temperature is lowered, the extraction condition is mild, and the stereochemical structure and the biological activity of the wolfberry polysaccharide can be effectively protected.

Description

technical field [0001] The invention belongs to a method for extracting plant polysaccharides, in particular to a method for extracting polysaccharides from Chinese medicinal material Lycium barbarum. Background technique [0002] Conventional methods for extracting plant polysaccharides are solvent extraction, ultrasonic extraction and microwave extraction. [0003] Among them, the solvent extraction method generally uses hot water, aqueous acid solution or aqueous alkali solution as the solvent, and the hot water extraction method is the most widely used in polysaccharide extraction. The solvent extraction process is relatively simple and easy to operate. However, its extraction efficiency is low, and the higher extraction temperature and the presence of acid and alkali easily destroy the structure of polysaccharides and reduce the biological activity of polysaccharides. [0004] Ultrasonic extraction is a physical crushing process, which can effectively increase the ext...

AI Technical Summary

Problems solved by technology

However, its extraction efficiency is low, and the higher extraction temperature and the presence of acid and alkali easily destroy the structure of polysaccharides and reduce the biological activity of polysaccharides.

[0004] Ultrasonic extraction is a physical crushing process, which can effectively increase the extraction rate, shorten the extraction time, and protect the active structure of polysaccharides, but the impurities will dissolve with the active ingredients, so the selectivity is poor, and at the same time, a large ultrasonic power is required, and the cost is high.

[0005] The advantages of the microwave extraction method are rapid heating, high efficiency and energy saving, and good selectivity. However, microwaves are harmful to the human body, and the problem of microwave leakage has become a key issue restricting the large-scale application of microwave technology in the extraction of active ingredients in natural medicines.