Method for extracting effective components in holothurian by means of salting out

A technology for active ingredients and sea cucumbers, which is applied in the field of double aqueous phase and three liquid phase extraction of sea cucumber active ingredients, can solve the problems of large loss of active ingredients, single target product, cumbersome operation process, etc., and achieves easy recovery, simple production process operation, The effect of fast mass transfer and phase separation

Active Publication Date: 2010-12-01
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the existing problems in the extraction of active ingredients of sea cucumbers such as single target product, large loss of other active ingredients, high cost, cumbersome operation process, long production cycle, and large solvent consumption, the present invention provides a hydrophilic low molecular weight A new type of two-phase system formed by organic matter / salt and a three-phase system of hydrophobic organic matter / hydrophilic low-molecular-weight organic matter / salt / water A method for simultaneously extracting and separating multiple active ingredients from sea cucumber cooking liquid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: sodium carbonate / ethanol system extracts active ingredient in sea cucumber

[0018] Step 1. Add anhydrous sodium carbonate and ethanol to the concentrated solution of sea cucumber processing cooking liquid to make the concentration reach 16% and 21% (w / w), stir and shake well, and place it in a water bath at 37°C for 10 minutes to form a two-water phase. And centrifuge at 5000rmp for 10min to make phase separation complete. Determination of protein and polysaccharide in each phase showed that 90.3% of sugar and 96.1% of protein were distributed in the solid phase.

[0019] Step 2: Take out the solid phase between the upper and lower phase interface layers, and freeze-dry it to make powder.

[0020] Step 3 digests the prepared powder sample, and carries out the determination of metal ions. The measurement results show that metallic mercury, lead, and arsenic are not detected; %; the content of selenium is 0.150mg / L; the content of calcium is 2785mg / L; the...

Embodiment 2

[0021] Example 2: Three-liquid-phase extraction of active ingredients in sea cucumbers

[0022] Step 1: Add anhydrous sodium carbonate and ethanol to the concentrated solution of sea cucumber processing cooking liquid to make its concentration reach 16% and 21% (w / w), then add n-hexane to make its concentration reach anhydrous sodium carbonate, ethanol, n-hexane 28% (w / w) of the total mass, stirred and shaken, placed in a water bath at 37°C for 10 minutes to form a three-liquid phase, and centrifuged at 5000rmp for 10 minutes to completely separate the phases. Determination of protein and polysaccharide in each phase showed that 96.9% of protein and 71.2% of sugar were distributed in the solid phase.

[0023] In step 2, take out the upper phase and evaporate n-hexane to obtain oil, and the extraction rate of sea cucumber oil obtained by three-liquid phase extraction is 63.3%.

Embodiment 3

[0024] Embodiment 3: Ammonium sulfate / tert-butanol system extracts effective components in sea cucumbers

[0025] Step 1 Add ammonium sulfate (30% w / v) and tert-butanol (1:1.5, sea cucumber processed cooking liquid v: tert-butanol v) to the concentrated solution of sea cucumber processing cooking liquid, stir and shake well, and let stand at room temperature for 0.5 h forms an aqueous two phase.

[0026] Step 2: Take out 187ml of the upper phase of the two-phase aqueous phase, and remove tert-butanol by rotary evaporation at 37°C to obtain the alcohol-soluble active ingredient of sea cucumber.

[0027] Step 3: Take out the solid phase between the upper and lower interface layers, and freeze-dry it to make powder.

[0028] In step 4, the prepared powder sample is digested with perchloric acid and concentrated nitric acid, and the determination of metal ions is carried out. The measurement results show that the content of harmful heavy metal cadmium is 0.620mg / L, which is reduced...

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Abstract

The invention discloses a method for extracting effective components in holothurian by means of salting out, belonging to the technical field of the further processing of marine products. The method comprises the following steps of: directly adding some electrolyte and common organic solvent into holothurian extracting liquid to form into an organic solvent/salt multiphase salting out and extracting system with the hydration of salt ion; separating and concentrating the effective components according to the difference of solubility and the different distribution of the different effective components with different polarities in the holothurian in each phase; adding salt and hydrophilic low-molecular organic matter into holothurian extract liquid according to a certain proportion, evenly stirring, stewing or decentralizing to form into double water phase; and adding the salt, the hydrophilic low-molecular organic matter and the hydrophobic organic matter into the holothurian extracting liquid, evenly stirring, stewing or decentralizing to form into a three-liquid phase salting out extracting system. The method develops a novel salting out extracting method which can simultaneously distill and separate various effective components in the holothurian, is simple in operation, moderate in condition and low in cost, and effectively reduces the toxic action of heavy metal.

Description

technical field [0001] The invention belongs to the technical field of deep processing of seafood, and relates to a method for extracting active ingredients from sea cucumbers by salting out, in particular to a method for extracting active ingredients of sea cucumbers in two aqueous phases and three liquid phases. Background technique [0002] Sea cucumber is a familiar seafood treasure, known as "sea ginseng", which has high nutritional and medicinal value. Sea cucumbers are not only rich in amino acids, essential fatty acids, vitamins and chemical elements and more than 50 kinds of nutrients needed by the human body, but also contain a variety of biologically active substances, such as sea cucumber saponins, sea cucumber polysaccharides, sea cucumber collagen, polyunsaturated fatty acids, brain Glycosides and sea cucumber peptides produced by enzymatic hydrolysis of sea cucumbers, etc. These ingredients have high nutritional value and medicinal value, such as: essential f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/333A23L1/28A23L17/50
CPCA23L1/30A23L1/333A23L17/50A23L33/10
Inventor 修志龙杨向春惠欢庆李晓晖
Owner DALIAN UNIV OF TECH
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