26 results about "Toxic Actions" patented technology

The invention discloses a method for inhibiting production of vomitoxin from bacillus cereus, which is characterized in that isoleucine is added into a substance needing to inhibit production of vomitoxin from bacillus cereus, preferably 5-20mM of isoleucine is added. According to the present invention, the content of the toxin is detected through the liquid chromatography-mass spectrometry, and the cytotoxicity experiment verification results show that the isoleucine can significantly inhibit the synthesis of the vomitoxin of the bacillus cereus in the culture medium and the matrix, and can effectively reduce the toxic effect of the toxin on HeLa cells; the invention provides a safe and efficient biological control means which is suitable for various food systems such as starch food, dairy products, meat products and the like. Isoleucine can be used as a novel non-toxic and harmless food additive and is low in cost; the use method is simple, the output of vomitoxin can be reduced by directly adding a trace amount of isoleucine, and the food flavor is not affected.

The invention discloses application of lanthanum carbonate in treatment of inflammatory bowel diseases, lanthanum carbonate achieves the effect of treating inflammatory diseases by depriving cfDNA, eATP and other proinflammatory factors generated at an inflammatory part, and symptoms induced by IBD are effectively relieved. The lanthanum carbonate disclosed by the invention has a strong cfDNA and eATP removal function, has good biocompatibility, has no direct or indirect toxic action on a human body, has no potential toxicity, and can be used for treating inflammatory bowel diseases.

The invention relates to a method for evaluating toxicity of spiroxamine by using zebra fish and application of the method, and belongs to the technical field of toxicity detection. According to the method, spiroxamine culture solutions with different concentrations are prepared for culturing zebrafish embryos, embryonic development detection and juvenile fish behavior testing are carried out, embryonic development detection includes hatching rate, heart rate and body length, and juvenile fish behavior testing includes spontaneous movement testing, vortex escape testing, swimming behavior testing, reflex behavior testing and color stimulation testing. According to the method provided by the invention, the toxic effect of spiroxamine on zebra fish embryos and juvenile fishes is systematically and comprehensively evaluated, the intuition and accuracy of spiroxamine toxicity detection are improved, and the method can be applied to evaluating the toxicity of environmental samples containing spiroxamine.

The application provides a method for analyzing the synergistic mechanism of hepatotoxicity and nephrotoxicity of non-steroidal anti-inflammatory drugs. The method comprises the following steps: preliminary toxicity prediction of NSAIDs and collection of toxicity target points, collection of liver and kidney disease target points, then cross and screening of the target points to obtain core target points and common core target points of NSAIDs induced liver and kidney diseases, and then constructing a protein interaction network of the common core target points; enrichment analysis of the common core target points to obtain the common action pathway of NSAIDs induced liver and kidney diseases; finally, further screening of the common core target points to obtain the key target points of NSAIDs induced liver and kidney diseases, and verification by using molecular docking technology. Compared with the traditional method, the advantages of the method are: first, the method does not depend on large-scale patient clinical data and a large number of animal or cell experiments, avoiding the ethical controversy in animal experiments and human experiments; second, the method can identify the potential cross-pathway and synergistic toxicity mechanism when a compound triggers multiple diseases, which is helpful for more comprehensive evaluation of the toxicity risk of NSAIDs.

DEPCT6825 / 04 / 2568 A synergistic biological pesticide compound that relies on essential oils. And the terpene component that should be used is geraniol, which has an effect on the Tetranychusurticae composition. The pesticide is active against all stages of mites and has a short estimation time with this mode. Non-toxic action and reduced delivery rate for each component of the inhibitor. Pesticides have been used to fight and control pests in plants; this invention has also provided a method for that. Fighting and controlling pests;

The application provides a repellent and contact-killing tick composition and a preparation method and application thereof. The repellent and contact-killing tick composition comprises plant essential oil and DEET; the repellent and contact-killing tick composition comprises a plant essential oil component; the plant essential oil and DEET are mixed to have a good synergistic effect; on the basis of the repellent effect of DEET, the contact-killing effect is increased. The repellent and contact-killing tick composition has a long repellent time, good safety, and reduced toxicity of DEET. The application screens plant essential oil with effective repellent and contact-killing effects, mixes the plant essential oil with DEET, reduces the DEET content in the repellent and contact-killing tick composition, reduces the toxic effect of DEET while having the same repellent effect, and increases the contact-killing effect of ticks. The repellent and contact-killing tick composition has a long repellent time and good contact-killing effect, can replace existing organic insect repellents and insecticides, and solves the problem of tick drug resistance.

This invention discloses a method for assessing the toxicity of antibiotics to green algae based on Fourier transform infrared spectroscopy, comprising: constructing a multidimensional experimental dataset containing different antibiotic concentrations and exposure times under controlled conditions, and simultaneously acquiring raw infrared spectra and degradation efficiency data of green algae. The spectral data are preprocessed to extract toxic response features characterizing cell damage and metabolic feedback features reflecting metabolic activity, forming a dual-path spectral feature set. A heterogeneous information network containing toxic effect edges and degradation feedback edges is constructed using antibiotic concentration and green algae state as nodes. A toxicity joint inference model is constructed based on a graph neural network, and the model is trained using the heterogeneous information network to achieve synergistic analysis of toxic effects and degradation potential. The trained model is used to analyze the target water area situation, simulate the purification process, and generate a dynamic river purification planning report including nodes for biomass maintenance and auxiliary intervention measures, providing comprehensive and accurate purification decision support.

This present invention provides C-TAB.G5 and C-TAB.G5.1 isolated polypeptides comprising the receptor binding domains of C. difficile toxin A and toxin B as set forth in the amino acid sequences of SEQ ID NO: 2 and SEQ ID NO: 4. The C-TAB.G5 and C-TAB.G5.1 isolated polypeptides may be used to neutralize toxic effects of C. difficile toxin A and / or toxin B.

The invention provides a plant growth promoter, which comprises tryptophan alcohol, and the weight percentage of the tryptophan alcohol in the plant growth promoter is 0.01%-10%. According to the plant growth promoter disclosed by the invention, tryptophan is taken as an effective component, plant growth can be well promoted by using a small amount of tryptophan, and the plant growth promoter is small in product amount, low in toxicity, small in residue, safe to use, environment-friendly, capable of greatly reducing toxic action on various natural enemies, small in damage and pollution to the ecological environment and beneficial to protecting safety of people and livestock.

The invention discloses a non-targeted extract screening and toxicity driving factor identification method based on IDA-UPLC-QTOF-MS / MS. Real extracts are obtained by simulating a real use scene and are subjected to chemical analysis, qualitative chemical characteristics are subjected to three-stage screening, then potential toxic action targets and mechanisms of the real extracts are revealed through calculation and simulation, and the toxicity driving factors of the real extracts are identified. A few toxicity driving factors which contribute to health risks are positioned, and a clear direction is provided for subsequent experimental verification, so that traditional high-flux toxicity screening is greatly replaced, and a large amount of research and development and detection cost is saved.

The invention discloses establishment and application of gobiocypris rarus eye cells, and the gobiocypris rarus eye cells Gbr-eye are constructed by utilizing the eyes of the gobiocypris rarus, and are gobiocypris rarus eye cell lines capable of realizing continuous passage. The culture method of the gobiocypris rarus eye cell line comprises the steps of primary culture, subculture, cryopreservation and recovery. A chemical and environmental water acute toxicity assessment method is established based on the gobiocypris rarus eye cell line, and the method specifically comprises the steps of inoculation of gobiocypris rarus eye cells, exposure of 3, 4-dichloroaniline and environmental water and toxic effect detection of 3, 4-dichloroaniline on the gobiocypris rarus eye cells. According to the establishment method and application of the gobiocypris rarus eye cells, the application of the gobiocypris rarus in toxicity detection of chemicals and environmental water bodies is expanded, and reference is provided for acute toxicity test based on fish cells.

The invention belongs to the technical field of biological medicine, and particularly discloses application of mannose in preparation of medicine for treating or preventing silicosis. The invention discloses application of mannose in preparation of medicines for treating or preventing silicosis for the first time. Tests show that the mannose has a good curative effect on relieving diseases of silicosis, has outstanding potential for treating silicosis, has no obvious toxic action on cells, is relatively good in safety, has a good medicinal value prospect, and can be developed into a novel medicine for treating silicosis.

The present application belongs to the technical field of biological toxicity test of chemicals, and particularly relates to a chemical toxicity test method based on a self-developed pollutant exposure system. The test method is used for simulating the toxicity of chemicals in natural water bodies by means of a self-developed pollutant exposure system, adopts zebrafish as a model organism, establishes a time-concentration linear function of the tested chemicals in a poisoning area, and calculates the toxicity test method of the minimum effect concentration of the tested chemicals by observing the abnormal behavior of zebrafish or analyzing the behavior by coupling a camera tracking technology. The test method is almost a non-destructive experiment, the experimental time is short, most of the zebrafish participating in the experiment can be restored to normal state through appropriate recovery, the time cost is greatly saved, and the test is efficient and has a low mortality rate of test organisms.

The invention discloses a Karenia mikimotoi red tide biotoxicity and risk assessment method, and belongs to the field of marine environment monitoring and ecological risk assessment. The method comprises the steps of karenia mikimotoi culture, marine model fish model establishment, karenia mikimotoi exposure, multi-system toxicity index detection and data analysis. By integrating five toxic pathways (hemolysis injury, detoxification metabolism, oxidative stress, immune inflammation and reproductive function), systematic evaluation from a micromechanism (gene / cell) to a macroscopic effect (tissue / individual) is realized, and a toxic action mechanism is comprehensively revealed. By means of LDH, blood sugar, ROS and other sub-lethal indexes, early warning is achieved before irreversible injury occurs, and more abundant response time is provided for red tide disaster prevention. Traditional phenomenon observation is broken through, multi-system dysfunction caused by energy depletion, immune attack and other new mechanisms of Karenia mikimotoi is revealed through a key gene expression mode, and a new target is provided for toxicity prevention and control.

The invention relates to the technical field of biological medicine, and provides application of PF-4708671 in preparation of a medicine for relieving aquilaria delavayi toxin. Aiming at the problems of lack of specific therapeutic drugs, difficulty in reversing cell injury, difficulty in controlling systematic toxicity and lack of intervention strategies for signal pathways in the field of PLTX treatment, the invention proposes that a small-molecule inhibitor PF-4708671 is used for reversing cell injury caused by PLTX for the first time on the basis of further exploring a toxic action mechanism of PLTX. It is found through experiments that the PF-4708671 can remarkably relieve related damage of cellular morphology, cytoskeleton, multiplication capacity, migration and invasion capacity and adhesion capacity caused after PLTX acts on HaCaT cells.

The invention discloses an iron-based catalyst for eliminating toxic action of sulfur element by calcium and sodium as well as a preparation method and application of the iron-based catalyst. The preparation method of the iron-based catalyst comprises the following steps: respectively preparing an aqueous solution of ferrous sulfate and a mixed aqueous solution of calcium nitrate and sodium nitrate, slowly dropwise adding the aqueous solution of ferrous sulfate into the mixed solution of calcium nitrate and sodium nitrate, magnetically stirring and reacting for a period of time under a water-bath heating condition; and cooling to room temperature, filtering, drying and crystallizing the filtrate, and then roasting and crystallizing to obtain the iron-based catalyst. According to the method, the content of the sulfur element in the catalyst is effectively reduced, the toxic action of the sulfur element on the catalyst is weakened, the prepared iron-based catalyst has the activity of catalyzing the CO2 hydrogenation reaction, the CO2 conversion rate is higher than 13%, and the C5 + hydrocarbon selectivity of the product is 55% or above.

The present invention discloses a nano-antibody for patulin and its application. The present invention uses an artificial patulin antigen (a conjugate of patulin and a carrier protein BSA) as a coating source and utilizes a natural nano-antibody library to screen the patulin nano-antibody. This method eliminates the need to immunize camelids with the artificial patulin antigen, thereby avoiding the toxic effects of patulin on animals. The patulin nano-antibody is prepared and a patulin immunoassay method is established using the patulin nano-antibody. The detection limit for patulin is 0.12 μg / mL, the half-inhibitory concentration is 0.41 μg / mL, and the linear range is 0.17-0.97 μg / mL. The method is simple, rapid, highly specific, and highly sensitive, and can be used for the rapid detection of patulin in food.

The invention particularly relates to a multi-receptor and multi-level chlorinated paraffin toxicity effect database and visual expression system. The multi-receptor and multi-level chlorinated paraffin toxicity effect database comprises a data storage module used for storing sorted chlorinated paraffin query data; the data preprocessing module is used for converting and filtering the data; the index creating module is used for carrying out preprocessing and word segmentation on the text and constructing an inverted index; the data retrieval module is used for quickly retrieving data meeting conditions from the index; the chlorinated paraffin accumulation degree module is used for generating and displaying an accumulation degree point diagram and an accumulation degree thermodynamic diagram of chlorinated paraffin in an organism; the biological lesion map module is used for dynamically generating and displaying a lesion map in the living body contacted with the chlorinated paraffin according to an experimental result; and the video visualization module is used for generating and displaying a toxic action mechanism network of the chlorinated paraffin. According to the method, the chlorinated paraffin toxicity data retrieval efficiency is remarkably improved, the toxic action mechanism is visually displayed, and a precise visual analysis tool is provided for toxicological research.

The invention belongs to the technical field of biotoxicity testing, and discloses branched chain fatty acid and application thereof in inhibiting and killing caenorhabditis elegans. Model organism caenorhabditis elegans are adopted as a tested organism, 3-methylvaleric acid is adopted as an exogenous medicine, an experimental group and a control group with different concentration gradients are set, the death rates of the caenorhabditis elegans in 24 hours, 48 hours and 72 hours are calculated, and the death rate of the caenorhabditis elegans in the experimental group is calculated. And an LC50 value is calculated. Apparent structure analysis and transcriptome test analysis are carried out by means of an electron microscope, fluorescent staining and the like, and the toxic action mechanism of exogenous drugs on nematodes is explored. The research systematically reveals the toxicity mechanism of 3-methylvaleric acid for the first time, and provides a theoretical basis for the future research and development of 3-methylvaleric acid.

The present invention discloses a 25-hydroxyvitamin D dissociating agent, which is characterized by comprising the following components: 10-500 mM PBS, 0.005-0.05 g / mL n-octyl-β-D-maltoside, 0.01-0.1 g / mL 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate, 0.001-0.01 g / mL sodium dodecyl sulfate, 0.001-0.01 g / mL BSA, 0.001-0.01 g / mL sodium chloride, 0.01-1% of PC300, and pH 6.0-8.0. The present invention has no toxic effect on the human body and no direct or indirect toxic hazards and risks to users and producers; most of the components of the present invention are novel and highly efficient detergents, and have an obvious effect on protein dissociation.

The present application relates to the field of immune cell therapy, and provides a chimeric antigen receptor based on the modification of the receptor tyrosine kinase family AXL and its application. The chimeric antigen receptor contains an extracellular binding domain, and the extracellular binding domain can specifically bind to the AXL ligand; the amino acid sequence of the AXL is shown in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5. Target specificity: GAS6 is lowly expressed on the cell membrane of normal cells, but highly expressed on the cell membrane of tumor tissues, so that the CAR provided by the present invention can specifically kill tumor cells with high expression of GAS6 on the membrane without obvious toxic effects on normal cells. The present invention utilizes the mode of action of ligand binding to receptor, rather than scFv in the traditional sense. The conservation of receptor-ligand interaction determines the safety test in animals, especially primates, which can better reflect its safety in humans.

This present invention provides C-TAB.G5 and C-TAB.G5.1 isolated polypeptides comprising the receptor binding domains of C. difficile toxin A and toxin B as set forth in the amino acid sequences of SEQ ID NO: 2 and SEQ ID NO: 4. The C-TAB.G5 and C-TAB.G5.1 isolated polypeptides may be used to neutralize toxic effects of C. difficile toxin A and / or toxin B.

The invention discloses an aquatic organism toxicity effect threshold value calculation method based on machine learning. The method comprises the following steps: step 1, constructing a chemical target toxicity endpoint feature database; 2, based on the database established in the step 1, selecting chemical molecule descriptors serving as input by adopting a feature screening method, and constructing a target toxicity endpoint prediction model; step 3, constructing a chemical toxic action mode database; 4, based on the database established in the step 3, screening chemical molecule descriptors serving as input by adopting a principal component analysis method, and constructing a toxic action mode classification model; step 5, based on the target toxicity endpoint prediction model and the toxic action mode prediction model, screening a reference compound of a target toxicity test type in the chemical mixture; and step 6, based on the reference compound, calculating the toxicity effect threshold value of the aquatic organism target toxicity end point. According to the method, a machine learning technology is adopted, based on a toxicological data set in a public database, derivation of the toxicity threshold value of the aquatic organisms is achieved, time and economic cost are saved, and a method is provided for water quality risk assessment and management and control.

The invention discloses establishment and application of gill cells of gobiocypris rarus, gill of gobiocypris rarus is utilized to establish gill cells Gbr-gill of gobiocypris rarus, and the gill cells Gbr-gill are gill cell lines of gobiocypris rarus which can be continuously passed. The culture method of the gobiocypris rarus gill cell line comprises the steps of primary culture, subculture and cryopreservation and resuscitation of the gobiocypris rarus gill cell line. A chemical and environmental water acute toxicity assessment method is established based on the gobiocypris rarus gill cell line, and the method specifically comprises the following steps: inoculating gobiocypris rarus gill cells, exposing 3, 4-dichloroaniline and environmental water, and detecting the toxic effect of 3, 4-dichloroaniline on the gobiocypris rarus gill cells. According to the establishment method and application of gill cells of gobiocypris rarus, the application of gobiocypris rarus in toxicity detection of chemicals and environmental water is expanded, and reference is provided for acute toxicity test based on fish cells.

The invention provides a method for analyzing a combined action mechanism of hepatotoxicity and renal toxicity of a non-steroidal anti-inflammatory drug. The method comprises the following steps: preliminary toxicity prediction of NSAIDs, toxic action target collection, liver and kidney disease target collection, target crossing and screening to obtain a core target and a common core target of the NSAIDs induced liver and kidney diseases, and further constructing a protein interaction network of the common core target. Carrying out enrichment analysis on the common core target spot to obtain a common action way of inducing the liver and kidney diseases by the NSAIDs; and finally, further screening the common core target spot to obtain a key target spot of the NSAIDs-induced liver and kidney disease, and verifying by using a molecular docking technology. Compared with a traditional method, the method has the advantages that firstly, the method does not depend on large-scale patient clinical data and a large number of animal or cell experiments, and ethical disputes in animal experiments and human body experiments are avoided; and secondly, the method can identify potential cross pathways and synergistic toxicity mechanisms when the compound causes various diseases, and is beneficial to more comprehensively evaluating the toxicity risk of the NSAIDs.

Provided herein is a method for treating or preventing abnormal protein folding and deposition in a subject by administering a low dose of compound (1) to the subject. Also provided herein is a method for counteracting or preventing the toxic effects of abnormally folded and aggregated protein amyloid beta by using a relatively undersupplied amount (inverse stoichiometric ratio) of compound (1) in order to achieve a more potent detoxifying effect. Also provided are its dosage forms and administration.