293 results about "Toxic Actions" patented technology

The methods described herein enable the evaluation of compounds on subjects to assess their therapeutic efficacy or toxic effects. The target of analysis is the underlying biochemical process or processes (i.e., metabolic process) thought to be involved in disease pathogenesis. Molecular flux rates within the one or more biochemical processes serve as biomarkers and are quantitated and compared with the molecular flux rates (i.e., biomarker) from control subjects (i.e., subjects not exposed to the compounds). Any change in the biomarker in the subject relative to the biomarker in the control subject provides the necessary information to evaluate therapeutic efficacy of an administered drug or a toxic effect and to develop the compound further if desired. In one aspect of the invention, stable isotope-labeled substrate molecules are administered to a subject and the label is incorporated into targeted molecules in a manner that reveals molecular flux rates through one or more metabolic pathways of interest. By this method, a comparison between subjects and control subjects reveals the effects of the chemical entity or entities on the biomarkers. This, in turn, allows for the identification of potential therapeutic uses or toxicities of the compound. Combinations of compounds can also be systematically evaluated for complementary, synergistic, or antagonistic actions on the metabolic pathways of interest, using the methods of the present invention as a strategy for identifying and confirming novel therapeutic or toxic combinations of compounds.

Aspects of the invention provide methods for harnessing the potential of proteins that occur naturally (e.g., in humans) and that have serious but finite toxicity. Aspects of the invention relate to a quantitative systems-biological and structural approach to design a class Mof chimeric proteins that avoid the toxicity of protein drugs while retaining their desired activities. In particular, chimeric proteins containing a variant form of a natural protein fused to a targeting moiety may be administered to a subject to target a signal (e.g., induction of apoptosis) to particular cells without having a generalized toxic effect

The invention enables high-throughput screening of compounds in living systems to detect unanticipated or unintended biological actions. The invention also allows for screening, detection, and confirmation of new indications for approved drugs. Screening and detection of toxic effects of compounds also can be achieved by using the methods of the invention. The methods comprise administering isotope-labeled substrates to a living system so that the label is incorporated into molecules in a manner that reveals flux rates through metabolic pathways thought to be involved in a disease. Comparisons between living systems exposed to compounds and living systems not so exposed reveals the effects of the compounds on the flux rates through the metabolic pathways. Combinations or mixtures of compounds can be systematically screened to detect unanticipated or unintended biological actions, including synergistic actions, in the same manner.

Compounds useful for countering undesired toxic effects to cells, tissues or organs having formula (I) wherein: Ar is a group of formulae (i) or (ii), n is O or, when Ar has formula (i) above, then n may also be 1, R is CN, -GC(S)NH2, -C(O)NHR3 or, when R1 is 4-NO2 and R2 is H or 3-OH, then R may also be a group of formulae (iii), (iv), (v), (vi) where R3 is H, phenyl, phenyl(lower alkyl) or pyridylmethyl; R1 and R2 are each independently H, OH, NO2 or, when R is CN, also CH3, F, or CF3, provided that both R1 and R2 are simultaneously H.

The present invention relates generally to the method of increasing the oral bioavailability, reducing chemotherapy induced toxicity and side effects, and improving the effectiveness of pharmaceutical agents that are poorly absorbed from the gastrointestinal tract. Specifically, the invention relates to poorly absorbed pharmaceutical drugs and converting them to orotate salts. The orotate salts of the drugs can be dosed at lower doses to provide the efficacy benefits of a higher dose, while reducing the drugs' toxic effects at lower doses. Additionally, the orotate salts of pharmaceutical agents have better clearance and reduce the potential for drug-induced hepatic toxicity. Therefore, an especially useful formulation of the orotate salt of the pharmaceutical agent can provide rapid and consistent action using a lower dose while reducing drug interactions and side-effects.

Compounds useful for countering undesired toxic effects to cells, tissues or organs having formula (I) wherein: Ar is a group of formulae (i) or (ii), n is 0 or, when Ar has formula (i) above, then n may also be 1, R is CN, -GC(S)NH2, -C(O)NHR3 or, when R1 is 4-NO2 add R2 is H or 3-OH, then R may also be a group of formulae (iii), (iv), (v), (vi) where R3 is H, phenyl, phenyl(lower alkyl) or pyridylmethyl; R1 and R2 are each independently H, OH, NO2 or, when R is CN, also CH3, F, or CF3, provided that both R1 and R2 are not simultaneously H.

The invention belongs to the technical field of bioremediation of marine oil pollution, and relates to an immobilized algal-bacterial symbiotic system for processing petroleum pollutants. The immobilized algal-bacterial symbiotic system is prepared by the following steps: (1) selection and axenic cultivation of algae species, firstly carrying out lysozyme and sodium dodecyl sulfate (SDS) sterile pretreatment on chlorella algae fluid, and then inoculating into a BG11 culture solution containing antibiotics to carry out sterile cultivation, so as to obtain sterile chlorella algae fluid for later use; (2) selection and cultivation of petroleum degrading bacteria; (3) preparation of composite carrier bacteria algae gel balls, namely immobilizing the sterile chlorella algae fluid obtained in the step (1) and the petroleum degrading bacteria obtained in the step (2) by adopting sodium alga acid and polyvinyl acetate (PVA) as immobilized carriers, and finally obtaining the immobilized algal-bacterial symbiotic system. The immobilized algal-bacterial symbiotic system can float on the water to carry out degradation of petroleum pollution, and is free of secondary pollution, free of toxic action on microorganisms, rapid in reaction speed, stable and reliable in operation, less in microorganism loss, and free of separation.

The invention relates to synthetic antibacterial peptide comprising polypeptide selected from SEQ ID NO:1-SEQ IDNO:13 amino acid sequences, and polypeptide of a functional equivalent obtained from the synthetic antibacterial peptide through cyclization, N-terminal and C-terminal modification, translation from L-type amino acid to D-type amino acid, and sequence terminal deletion. The synthetic antibacterial peptide is synthesized by a solid phase chemical method. The synthetic antibacterial peptide has broad-spectrum killing activity to gram negative bacteria, gram positive bacteria and epiphytes, has stronger bactericidal activity than natural antibacterial peptide, has no toxic action on animal and plant cells, and can be used for preparing medicaments for curing infectious diseases.

The invention discloses a surface modifying method for improving the antibacterial property and the biological activity of medicinal titanium, which comprises the following steps of: feeding the titanium in hydrogen peroxide and carrying out hydro-thermal treatment at the temperature of 70-90DEG C; and injecting silver ions on the surface of the titanium subjected to the hydro-thermal treatment by using a plasma immersion ion injection technology (PIII). The surface of the titanium subjected to the modification treatment is covered by nanometer titanium dioxide with different morphologies; and after silver is injected, silver nano-particles which have particle size of about 10nm and exist in an elemental form are distributed on the surface of the titanium dioxide. A nanometer titanium dioxide layer can endow the titanium with osteogenic potential; and due to the existence of the silver nano-particles, the antimicrobial activity of the titanium can be remarkably improved. Therefore, the titanium subjected to modification treatment in the invention is a novel implant material with biological activity and antibacterial property, has no toxic action on cells as well as has practical value.

Methods and compositions for protecting and treating a muscarinic receptor in a subject in need thereof from dysfunction not resulting from oxidative stress, toxic actions of metals or metal ions, or infectious agents by administering a pyrophosphate analog.

The invention discloses a manufacturing method and process device of a reactivated cleaning agent for regeneration of a honeycomb SCR (selective catalytic reduction) denitration catalyst. The deactivated SCR denitration catalyst in the process device is regenerated through the steps of pressurized water spraying, ultrasonic cleaning, secondary pressurized water spraying, acid washing, forced air drying, active component supplementation, secondary forced air drying, step-by-step calcination and the like in sequence. By using (NH4)2SO4 as a main component of an acid washing solution, potential hazards caused by sulfuric acid are avoided, and the economic performance and the safety are realized. The components such as EDTA (ethylene diamine tetraacetic acid) are added into the cleaning agent, and under the chelation of the EDTA and heavy metal elements, the toxic action on the denitration catalyst caused by the heavy metal elements can be well eliminated. According to the process, the denitration activity of the deactivated catalyst can be recovered, and meanwhile, the structural strength of the catalyst can be kept. The process device is simple, suitable for regeneration in hoisting transportation, and high in efficiency. By the promotion of the process, the performance of the regenerated honeycomb SCR catalyst can be improved, the replacement cost of the catalyst is saved, and the process has an important significance on NOX control.

The invention relates to an experimental method for analyzing the toxicity and the effect of a skin whitening agent through a primary culture human being skin melanocyte. The method comprises the following steps: 1, culturing and identifying the primary human being skin melanocyte; 2, carrying out the analysis of the toxicity of skin whitening substances through the primary human being skin melanocyte; and 3, carrying out the analysis of the effect of the skin whitening substances through the primary human being skin melanocyte, namely carrying out the analysis on two aspects of the melanin content and the tyrosinase activity. The method has the following advantages that: the human being melanocyte cultured in vitro has a high division and multiplication capacity, a high standardization degree, little difference between batches, and the same function of producing melanin granules with the melanocyte in vivo; the result obtained through the healthy human being melanocyte is more reliable than the result obtained through animal or human-derived melanoma cells; and the toxic action and the whitening effect of the substances to be tested are evaluated from the two aspects of characterization and quantification. The method can replace the living body animal and human being skin, and can be directly applied to toxicity and effect experiments of the whitening substances in products such as chemicals, cosmetics, medicaments, and the like.

Methods, compositions and kits are provided for generating inner ear cells in vitro. These methods find use a number of applications, such as in preparing inner ear cells for in vitro screening for agents that are toxic to inner ear cells, for in vitro screening for agents that prevent against, mitigate, or reverse the toxic effects of such agents, and for in vitro screening for agents that promote otoregeneration.

The invention discloses a system for treating overproof radioactive laundry waste water, which comprises a bag filter, an active carbon filter and a zeolite filter; the outlet of the bag filter is connected with the inlet of the active carbon filter; and the outlet of the active carbon filter is connected with the inlet of the zeolite filter. According to the system for treating the overproof radioactive laundry waste water, the bag filter can effectively remove suspended solids and large particle impurities in laundry waste water; the active carbon filter can remove surface active agent and micromolecule organics in the waste water to further retain the suspended solids in the water and can eliminate toxic action of organic macromolecule such as the suspended solids generated during washing clothes, synthetic detergent in the waste water and the like; the zeolite filter has good ion exchange characteristic and can effectively remove radionuclide ions (cesium and strontium) in lower radioactive waste water. The system for treating the overpoorf radioactive laundry waste water is simple in structure, low in energy consumption and operating cost and high in treating quality for the overproof radioactive laundry waste water.

This invention belongs to chemistry pharmaceutical field and relates to a medication transfer system, especially relates to an agglutinin masked medication-load transfer mechanism transferring from nose to brain. This invention comprises medication carrier nanograin, vesicle or lipid body surface finish agglutinin. By using the transfer system, resort time of medication-load system on nasal mucosa can be prolonged, dielectric mucosa absorb the medication-load system and selectively deliver small molecular chemistry medication, diagnosis medication, polypeptide proteins and gene medication into brain. This invention can deliver more medication into brain and relatively decrease medication in outer tissue, so it can depress toxic action at every pore while toning up prevention, cure and diagnosis effect of backbone diseases.

The invention discloses a material for sewage treatment, as well as a preparation method and applications thereof. The material for sewage treatment is prepared from high-entropy metal glass containing at least one alkaline earth metal element. The high-entropy metal glass contains the alkaline earth metal with high chemical reaction activity, the alkaline earth metal can have oxidation-reduction reaction with N=N double bonds in an azo dye so as to further damage the N=N group having toxic action to organisms. By applying the high-entropy metal glass into treatment of sewage, especially industrial waste water containing the azo dye, higher degradation rate can be achieved, and the better sewage treatment function can be realized. In addition, the high-entropy metal glass has excellent glass forming capability within the wide constitution range, so that the degradation rate of the high-entropy metal glass in the practical application can be regulated more easily by regulating the proportion of raw ingredients. Raw ingredients for synthesizing the high-entropy metal glass are lower in price and lower in melting point, and the melting energy consumption can be greatly saved.

The invention discloses a microbial agent for producing nonantigenic soybean meal and a preparation method thereof and belongs to the technical field of microorganism application. The microbial agent uses Bacillus subtilis strain WB117, Saccharomyces cerevisiae Meyenex Hansen strain WY238 and Lactobacillus sp. strain L1 as strains. The strains are prepared into single-strain microbial agents respectively through inoculation, fermentation and culture; and the single-strain microbial agents are blended according a weight ratio of 1-3:1-3:1-3 to form a composite microbial agent. The microbial agent ferments a soybean meal raw material so as to remove antigenic substance and anti-nutritional factors, reduce the toxic action of the soybean meal on livestock, promote digestion and absorption, and improve percentage of the soybean meal in feed and the nutritional value of the soybean meal. The microbial agent and the method can be popularized among and used in feed additive enterprises and livestock farms or peasant households.

The invention discloses a method for extracting effective components in holothurian by means of salting out, belonging to the technical field of the further processing of marine products. The method comprises the following steps of: directly adding some electrolyte and common organic solvent into holothurian extracting liquid to form into an organic solvent / salt multiphase salting out and extracting system with the hydration of salt ion; separating and concentrating the effective components according to the difference of solubility and the different distribution of the different effective components with different polarities in the holothurian in each phase; adding salt and hydrophilic low-molecular organic matter into holothurian extract liquid according to a certain proportion, evenly stirring, stewing or decentralizing to form into double water phase; and adding the salt, the hydrophilic low-molecular organic matter and the hydrophobic organic matter into the holothurian extracting liquid, evenly stirring, stewing or decentralizing to form into a three-liquid phase salting out extracting system. The method develops a novel salting out extracting method which can simultaneously distill and separate various effective components in the holothurian, is simple in operation, moderate in condition and low in cost, and effectively reduces the toxic action of heavy metal.

The invention discloses a ratio-type two-photon formaldehyde fluorescent probe, a preparation method therefor and use of the ratio-type two-photon formaldehyde fluorescent probe. The ratio-type two-photon formaldehyde fluorescent probe has a structure as follows shown in the description. Molecules of the fluorescent probe disclosed by the invention show relatively high selectivity and sensitivity to formaldehyde in a coexisting system of molecules of the formaldehyde and other aldehydes, amino acids and the like. Proven by cytotoxicity tests, the molecules of the fluorescent probe disclosed by the invention are almost free of toxic action on cells; and shown by two-photon fluorescence microscopy imaging experiments, the molecules of the fluorescent probe disclosed by the invention have good cell permeability to MCF-7 cells, so that the fluorescent probe is applicable to the detection of presence of formaldehyde molecules in the cells.

The invention belongs to the technical field of biological pesticides and discloses a method for identifying a Streptomyces glaucus sp.nov yangling TIASA5 strain and a method for preparing an active substance of the strain and applications of the method. The strain is preserved in China General Microbiological Culture Center of Microbial Culture Collection Management Committee in Microbiology Institute, Chinese Academy of Science on August 16th, 2007 with the preservation number of CGMCC No.2132. The strain has better preventive and therapeutic effects on bacterial canker of kiwi fruits. The strain is separated from plant tissues, generates active metabolite that does not have toxic action to the plant tissues, and has stability, convenient spraying and convenient popularization and application.

The invention relates to a method for promoting growth of sweet sorghum in a saline-alkali land with arbuscular mycorrhizal fungi and an organic fertilizer, sweet sorghum seeds are sown in the saline-alkali land, the arbuscular mycorrhizal fungi are inoculated and the organic fertilizer is applied simultaneously, and conventional field management is performed in the growing process of the sweet sorghum until harvesting. According to the method, the buffering performance and adsorption capacity of the soil can be enhanced by the aid of the actions of adsorption, complexing, reduction, volatilizing and the like of the organic fertilizer, the toxic action of saline ions is reduced, and the plant growth is promoted; the effectiveness of the saline ions is reduced by the aid of the chelation, the hypha immobilization function and the like of the arbuscular mycorrhizal fungi, the plant tolerance is improved in various ways of improving the plant nutriture, changing the rhizosphere physical and chemical properties, changing the root morphology, adjusting the expression of some salt-resistant genes and the synthesis of related proteins and the like, and the arbuscular mycorrhizal fungi and the organic fertilizer are applied simultaneously, so that saline-alkali stress can be relieved synergistically.

The invention discloses an engineering bacteria for producing 1,3-propylene glycol and a method for constructing the same. The invention silences the phosphate transacetylase genes in a wild type strain producing 1,3-propylene glycol by utilizing a homologous recombination method and a gene insertional inactivation method so as to obtain a genetically engineered bacterium, of which the acetic acid metabolic pathway is blocked. When the 1,3-propylene glycol is fermented and produced by the engineering bacteria disclosed by the invention, the acetic acid production is largely reduced, the toxic action on cells caused by the by-product acetic acid is greatly decreased and the production rate of biomass per unit is enhanced. Moreover, the post-extracting process is simplified and the production cost is reduced as the species of the by-products are reduced. The experiments prove that the concentration of the 1,3-propylene glycol can reach more than 55g / L by fermenting the engineering bacteria provided by the invention for 28 hours according to a conventional method. The invention plays an important role in the industrial production for producing 1,3-propylene glycol by a microbiological fermentation method and has a wide application prospect.

The invention discloses a method for quantitatively evaluating medicament toxicity by using metabonomic technology, which is characterized by comprising the following steps: comprehensively and quantitatively measuring small molecule compounds in a biological sample by using the measuring technology based on mass spectrum, nuclear magnetic resonance and the like, then establishing a multi-dimensional spatial mathematical model by adopting a multi-variable data processing method, calculating a relative distance between an administration group and a control group and taking the relative distance as a quantitative index for evaluating the medicament toxicity so as to solve the difficult problem that the medicament toxicity evaluation lacks the quantitative evaluating index. Compared with the conventional toxicity evaluating method, the method has the advantages of wide application, sensitivity, simple and convenient sampling, no harm to the body, and capability of reflecting the toxicity function more comprehensively, and providing a comprehensive and reliable quantitative evaluating method for toxicity evaluation in new medicament research and development and pharmacologic research.

The invention provides a tilapia feed taking cotton seed meal and rapeseed meal as main protein materials, belonging to the technical field of fish feed. The tilapia feed is characterized by comprising the following components by weight percent: 10-26 percent of cotton seed meal, 30-50 percent of rapeseed meal, 0-20 percent of soybean meal, 15-40 percent of sub-powder, 0.1-1.5 percent of tangerine peel, 0.3-1.5 percent of dry leaves of eucommia bark, 0.5-4 percent of calcium hydrophosphate, 0.1-0.3 percent of compound vitamin, 0.1-0.5 percent of compound trace ore element, 0.1-0.5 percent of salt and 0.5-3 percent of compound premix. The tilapia feed obviously reduces the toxic action of biological toxin substances in the cotton seed meal and the rapeseed meal to the tilapia, obviously improves the meat quality of the tilapia, and is not added with any antibiotics, hormone and veterinary drug; the growth speed of the tilapia is fast, the feed coefficient is low, and the feed has good stability in water, and does not pollute aquaculture water bodies easily; and the materials are simple and easily obtained, the cost is low and the aquaculture cost can be greatly reduced.

The invention discloses a voriconazole slow-release suppository and a preparation method thereof. The voriconazole slow-release suppository disclosed by the invention is prepared from voriconazole, a solubilizer, a slow-release material, an emulsifier, a dispersing medium and an excipient; and the preparation method disclosed by the invention comprises the following steps of: preparing slow-release microspheres by solubilizing the extremely indissolvable voriconazole, carrying out cast pouring and cooling to obtain the voriconazole slow-release suppository. The preparation method disclosed bythe invention is simple, the prepared suppository has a slow-release characteristic, the first pass effect generated by oral administration and the toxic action of intravenous administration for the organs of a whole body are avoided, and particularly for the patients of intensive care units, the patients with serious fungous infection and child and senile patients, the pain brought to the patients by frequent administration is reduced, the administration compliance is improved, and the curative effect of medicaments is improved.

The invention discloses a method for preparing a polycarboxylic acid water reducing agent by using sodium methyl acryl sulfonate as a chain transfer agent. The method comprises the following steps of: firstly, adding water, sodium methyl acryl sulfonate and modified polyether in a reaction kettle; then mixing sodium methyl acryl sulfonate and acrylic acid with water to prepare an acrylic acid solution, mixing water with ammonium persulfate to prepare an ammonium persulfate solution; when the temperature of materials reaches 60 DGE C+ / -2 DEG C, simultaneously dropwise adding the ammonium persulfate solution and the acrylic acid solution; and carrying out heat insulation reaction for 1-3 hours and when cooling below 45 DEG C, adding 32% liquid caustic soda for neutralization so as to obtain the polycarboxylic acid water reducing agent having the PH value of 6-7 and 40% of solid content. Because sodium methyl acryl sulfonate is used as the chain transfer agent instead of mercaptoacetic acid or mercaptopropionic acid in the preparation process of the polycarboxylic acid water reducing agent, environment pollution and toxic action on a human body caused by stink odor and strong corrosivity of mercaptoacetic acid or mercaptopropionic acid can be avoided.

The invention discloses a novel ocean antifouling paint which comprises the following materials according to parts by weight: 32-45 parts of polymer resin, 1-10 parts of biological antifouling composition, 33-45 parts of filler, 15-25 parts of solvent and 7-20 parts of auxiliary material, and the biological antifouling composition is capsaicine, cnidium lactone, elatol and protease. The capsaicine has an excellent effect on expelling marine organism, the cnidium lactone can effectively inhibit the adhesion of minitype marine fouling organism, large soft marine fouling organism and large hard marine fouling organism, the elatol has a better antifouling activity for the marine bacteria and invertebrate, and the protease can hydrolyze a peptide bond of a protein in a biological binder, so that the cohesive action of the adhered animals and plants is broken, but no toxic action is exerted on the adhered animals and plants.

The invention relates to a total-effect clothing pre-washing agent free from an organic solvent. The organic solvent has different degrees of toxic actions to the human body, is combustible and easy to explode, pollutes the environment and has obvious limitation. The total-effect clothing pre-washing agent disclosed by the invention is prepared from an anionic surfactant, a nonionic surfactant, an emulsifier with an HLB value of 5-13, aids, a short chain surfactant, a pH value regulator, a hydrotropic agent, a preservative, essence and water, wherein the emulsifier with the HLB value of 5-13 replaces a common organic solvent and is scientifically compatible with detergents and other aids, oily dirt is removed in emulsifying and dispersing modes, the ratio of the emulsifier to the detergent is between 1:10 and 2:1, an effect of removing multiple stains is achieved, and the stain removal ratios of standard carbon black stained cloth, protein stained cloth and sebum stained cloth are all over 1.0.

The invention relates to an emulsified soybean protein feed, comprising the following raw materials in parts by weight: 1-90 parts of animal and plant emulsified oil for a feed and 10-99 parts of plant dregs. The production method comprises the following steps: in the raw materials for forming the emulsified soybean protein feed, firstly, putting the emulsified oil and plant dregs into a mixer; inputting the prepared mixture into a bulking machine to puff under the agitation state, so as to obtain the emulsified soybean protein feed prepared from the animal and plant emulsified oil for the feed and the plant dregs; finally, crushing the obtained emulsified soybean protein feed with a crusher, screening by a 20-mesh sieve and packaging. By adopting the method, the plant dregs are mixed with the emulsified oil and then puffed, so that generation of urease can be reduced, anti-nutritional factors are removed, the problem of nutrient loss does not exist in the puffing process, the emulsified soybean protein feed is free of chemical residue, and safe and harmless, the toxic action of the bean pulp as an animal feed on beasts and birds is reduced, the digestibility of the animals on the feed is improved, and the storage time is also effectively prolonged.