Endogenetic streptomycete for preventing kiwi berry bacterial canker and preparation thereof

A kiwifruit and bacterial technology, applied to the field of endophytic Streptomyces and its preparation, can solve problems such as unsatisfactory field disease prevention effect, and achieve the effects of good antibacterial activity, simple separation and screening method, and good water solubility

Inactive Publication Date: 2009-07-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the medicaments used to prevent and control kiwifruit canker are mainly agricultural streptomycin and lime sulfur mixture. Shinaning is a new pesticide for the prevention and treatment of kiwifruit canker popularized by various plant protecti

Method used

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  • Endogenetic streptomycete for preventing kiwi berry bacterial canker and preparation thereof
  • Endogenetic streptomycete for preventing kiwi berry bacterial canker and preparation thereof
  • Endogenetic streptomycete for preventing kiwi berry bacterial canker and preparation thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1: Isolation and morphological identification of the TIASA5 strain

[0030] 1) Wash the healthy wild Artemisia annua stem tissue collected from Yangling, Shaanxi with water, blot the water dry with absorbent paper, soak in 99% ethanol for 1 min, then soak in 3.125% NaClO for 6 min, and put it in again Soak in 99% ethanol for 30 sec, and finally rinse with sterile saline for 5 times;

[0031]2) Cut the sterilized Artemisia annua stem tissue to be separated into 0.2mm long pieces with a sterilized blade, place them on the Gaoshi No. 1 plate, culture them in an incubator at 28°C for 21-28 days, and count the number of actinomycete colonies And purify into a single colony by streaking method;

[0032] 3) The purified single colony is fermented as follows: use a puncher to punch the bacterial lawn plate that has been activated and cultured on the soybean flour plate for 5 to 7 days into a 6mm diameter bacterial cake, insert it into the soybean flour medium, and heat...

Embodiment 2

[0033] Embodiment two: the preparation method of the antibacterial active substance of TIASA5 bacterial strain

[0034] The TIASA5 strain that was activated and cultivated on the soybean flour plate for 5 days was punched into a bacterium cake with a diameter of 6 mm, inserted into the seed medium, and cultivated for 36 hours at 28 ° C and 150 r / min. The solution was introduced into the fermentation medium, and fermented at 30°C and 180r / min for 72h. Centrifuge the cultured fermentation broth at 10,000 r / min for 30 minutes, and take the supernatant, which is the crude fermentation broth. The crude fermentation broth was treated with 1M H 2 SO4 adjusted to pH2, 80°C water bath for 1h, then adjusted to pH7 with 1M NaOH, then centrifuged (10000r / min, 30min), collected the supernatant, and preliminarily removed the protein and CaCO in the fermentation broth 3 . Put the pretreated fermentation broth on 732 # Ion exchange resin, dynamically adsorbed, and then eluted with 1N ammo...

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Abstract

The invention belongs to the technical field of biological pesticides and discloses a method for identifying a Streptomyces glaucus sp.nov yangling TIASA5 strain and a method for preparing an active substance of the strain and applications of the method. The strain is preserved in China General Microbiological Culture Center of Microbial Culture Collection Management Committee in Microbiology Institute, Chinese Academy of Science on August 16th, 2007 with the preservation number of CGMCC No.2132. The strain has better preventive and therapeutic effects on bacterial canker of kiwi fruits. The strain is separated from plant tissues, generates active metabolite that does not have toxic action to the plant tissues, and has stability, convenient spraying and convenient popularization and application.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to an endophytic streptomyces for preventing and treating kiwifruit bacterial canker and a preparation method thereof. Background technique [0002] Actinidia bacterial canker is an important disease on kiwifruit caused by Pseudomonassyringae pv. Cause damage garden, bring huge loss to kiwifruit industry. Kiwifruit bacterial canker was first discovered in 1980 in California, the United States and Jinggang County, Shenzhou, Japan. The disease was discovered in Dongshanfeng Farm in Hunan, my country in 1985, causing more than 2,000 mu of orchards to be on the verge of destruction. Kiwi fruit is widely planted in Shaanxi, Anhui and other provinces in my country. Kiwi fruit production is also one of the important fruit industries in Shaanxi Province. In recent years, the disease has a tendency to expand and spread, which has brought a serious crisis to the kiw...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/52A01N63/02A01P1/00C12R1/465
Inventor 黄丽丽涂璇康振生高小宁申哲
Owner NORTHWEST A & F UNIV
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