38results about How to "No obvious toxic effect" patented technology

The invention discloses a preparation method of a zinc oxide-graphene oxide composite nanomaterial, which is implemented through the following steps: by taking soluble divalent zinc ions as a zinc source, taking the soluble divalent zinc ions, graphene oxide and alkali liquor as reactants together, according to a situation that the mass ratio of the zinc ions to the graphene oxide is (1-3): 1, forming a solute composition; by taking alcohol as a solvent, forming a reaction solution mixed system; and after a reaction is performed for 30 min-5 h, purifying a substrate by using centrifugation and washing methods, so that the zinc oxide-graphene oxide composite nanomaterial is obtained. The zinc oxide-graphene oxide composite nanomaterial prepared according to the invention has an effective antibacterial ability at low concentration, and has no obvious toxic action on cells. According to the invention, the operation process is simple, the reaction process has no obvious pollution to the environment, the production cost is low, and the prepared nanomaterial can be applied to the antisepsis of medical apparatuses and instruments, and has a potential in commercial large-scale production.

The invention discloses a rhodamin B double-sulfur fluorescence probe for detecting aqueous mediums and intracellular mercury ions, preparation and application thereof. According to rhodamin B double-sulfur fluorescence probe, molecular closed loops in the rhodamin fluorescence probe are opened in the aqueous mediums by chelating and coordinating the mercury ions, so that fluorescence is generated; the fluorescence intensity is in a good linear relation with the initial concentration of the mercury ions; the color change can be seen by naked eyes when a probe solution is changed from colorless to pink; meanwhile, the fluorescence probe is high in specific selectivity on the mercury ions; in addition, the fluorescence probe is high in biocompatibility and cell permeability and low in cytotoxicity, can be used for detecting the intracellular mercury ions and is high in detection sensitivity of the mercury ions. The preparation of the rhodamin B double-sulfur fluorescence probe is characterized by taking the rhodamin B as the raw material, and enabling the rhodamin B to react with phosphorus oxychloride to prepare a rhodamin B acyl chloride intermediate; the acyl chloride intermediate is further condensed with a double-sulfur intermediate to obtain a mercury ion probe with high sensitivity and high selectivity.

The invention relates to a medicinal preparation for treating benign prostate hyperplasia and process for its preparation, wherein the medicament is prepared from astragalus root, peach kernels, zedoary, rhubarb horsetails, poria cocos, coix seed, motherwort, selfheal, Cinnamomum cassia, root of ballon flower, subprostrate sophora root, achyranthes and cyathula root, and medicinal auxiliary materials.

A Chinese medicine for preventing and treating hepatic fibrosis is prepared from cordyceps, astragalus root, red sage root, fleece flower root, wolfberry fruit and pseudostellaria root through extracting to obtain liquid, and including the liquid extract by liposome. Its advantages are sure curative effect and high safety.

The invention discloses application of panax japonicus extract in preparing medicine of preventing osteoporosis. The panax japonicus extract disclosed by the invention is extracted from natural traditional Chinese medicine panax japonicus; the panax japonicus is a common Chinese herbal medicine. According to the long-term traditional Chinese medicine application experience, the panax japonicus is high in using safety and free of remarkable toxic effects. The panax japonicus extract is remarkable in preventing osteoporosis, free of hepatotoxicity and safer than the existing phosphorus tablet. Therefore, the panax japonicus extract has a prospect of being developed into the medicine of preventing osteoporosis.

The invention relates to application of a Herba Ecliptae extract to preparation of anti-osteoporosis multi-target medicines or healthcare products.The Herba Ecliptae extract is obtained by steps: adding Herba Ecliptae to ethanol, using a Waring blender for blending to a uniform state, performing ethanol extraction, separating and collecting extract liquid, subjecting solid to ethanol extraction again, mixing the extract liquid obtained by two times, performing vacuum concentration to obtain an alcohol extract, adding water into the alcohol extract, and ultrasonically heating to realize dissolution; adding petroleum ether into dissolved solution, and collecting a water-phase part; adding ethyl acetate for extracting the water-phase part to obtain an ethyl acetate part, and performing vacuum concentration and drying to obtain the Herba Ecliptae extract.According to experiments, the Herba Ecliptae extract has an evident inhibition effect on bone resorption of osteoclasts, and in a same dosage range, osteogenic differentiation and mineralization of osteoblast precursors and mesenchymal stem cells are promoted, so that the Herba Ecliptae extract has dual-function multi-target functions in bone resorption inhibition and bone formation promotion and has a prospect of being developed into novel anti-osteoporosis medicines.

The invention belongs to the technical field of marine organisms, and relates to a preparation for removing epiphytic algae from an alga body and an application thereof. The preparation for removing adherent unwanted algae from an alga body is a plant herbicide. The preparation for removing adherent unwanted algae from an alga body is mixed with seawater, and then the alga body is immersed in seawater to remove adherent unwanted algae, wherein 1-5 g of preparations are added into 1 liter of seawater, and the immersion time is 5-10 minutes. The preparation for inhibiting epiphytic algae of theinvention is a plant herbicide; the action mechanism is that primary reactions of photosynthesis of epiphytic unwanted algae is inhibited, and PSII and the shikimic acid cycle at the photosynthetic reaction center, which prevent metabolic disorder due to drug absorption of glue-producing algae, are mainly inhibited; the preparation of the invention has strong specificity, is economical and effective, has simple operation; epiphytic unwanted algae on the alga body are observed to be withered, shrunk, dry and dead within several days; the purposes of inhibiting epiphytic unwanted algae and keeping glue-producing algae alive are reached; and the contaminated glue-producing algae are recovered to have smooth surfaces and can grow healthily again.

The invention relates to a medicine for treating benign prostatic hyperplasia and a preparation method thereof. Composed of pharmaceutical excipients. The preparation method is to crush cinnamon, rhubarb, coix seed, and peach kernel into fine powder; extract volatile oil from curcuma decoction pieces; Decoction pieces, zedoary dregs, water-extracted filtrate and astragalus ethanol liquid are combined and concentrated to make a dry paste, crushed, sieved, added zedoary volatile oil and fine powder of cinnamon and other drugs, and made into various preparations with medicinal excipients. The preparation can obviously inhibit the hyperplasia of prostate tissue induced by testosterone, significantly reduce the wet weight and dry weight of each leaf of the prostate, has no obvious toxic effect, and is safe and reliable; the preparation method has high bioavailability, good drug effect, stable quality and easy control.

The invention provides a traditional Chinese medicine preparation for external use for treating stroke and a preparation method thereof. The components of the traditional Chinese medicine are reasonable, synergy and interaction of various medicine effects are achieved, and the effects are significant. Qi-tonifying drug and blood-activating drug are matched to make qi vigorous, promote blood circulation and invigorate the blood circulation, and human bodies cannot be affected; phlegm reduction and brain strengthening drug is added and has an assistant effect to reinforce qi and nourish blood, warm and activate meridian, and eliminate phlegm and strengthen brain. The effects of promoting qi circulation, invigorating the blood circulation, eliminating phlegm, promoting blood circulation, andtreating stroke are achieved. According to the traditional Chinese medicine preparation, a hydrogel dosage form for external use is preferred, medicine efficacy can directly treat the illness position, the situations that after oral administration, milder medicine efficacy may cause endogenous heat evil and tepidity and yin impairment can be avoided, the use is convenient, peroral misery is avoided, and the traditional Chinese medicine preparation is particularly applicable to paralytic patients. According to the traditional Chinese medicine preparation, the transdermal absorption is achieved,continuous administration is performed, the transdermal release and adsorption are good, and significant effects of treating stroke symptoms such as facial paralysis, drooling, stiff tongue and speaking difficulty, numb muscles, arthralgia and myalgia and the like which are caused by wind-evil and collateral stroke are achieved.

The invention discloses a method for evidently increasing Litsea cubeba cluster bud rooting rate. The method includes the steps of firstly, separating Litsea cubeba cluster buds to obtain single buds;secondly using an anti-browning agent perform soaking pretreatment on the base cut of each single bud, wherein the anti-browning agent comprises astaxanthin; thirdly, inoculating the pretreated single buds to a seedling strengthening culture medium to perform seedling strengthening culture; fourthly, transferring the single buds after the seedling strengthening culture to a primary culture mediumto perform primary rooting culture to induce root primordium formation; fifthly, transferring the single buds after the primary rooting culture to a subculture medium to perform rooting subculture toobtain rooted seedlings, wherein the subculture medium comprises Litsea cubeba root tip tissue extract. The method has the advantages that cluster bud death caused by tissue browning during culture can be reduced greatly, the rooting efficiency and root growth speed of the Litsea cubeba cluster buds are increased evidently, Litsea cubeba rapid propagation efficiency is increased, and Litsea cubeba industry development is promoted.

The invention belongs to the field of agriculture and in particular relates to a seed soaking medicine composition which is prepared from the following raw materials in parts by weight: 20-30 parts oftea leaves, 15-18 parts of a tobacco extract, 1-3 parts of fennel, 1.5-2.5 parts of tea saponin, 1-3 parts of purple sweet potato anthocyanin, 4-6 parts of citrus peel, 10-15 parts of kelp powder, 8-12 parts of acanthopanax roots, 1-5 parts of wormwood, 1-6 parts of tetrapanax papyriferus, 5-8 parts of coal ash, 2-3 parts of a water retaining agent, 1-2 parts of tomato juice, 4-9 parts of cassiabarks and 3-8 parts of sesame oil. Tests on the seed soaking medicine composition provided by the invention show that compared with normal seeds, seeds soaked with the composition are very high in insect expelling performance and germination performance, and the composition is capable of effectively protecting crop seeds, expelling insects and reducing pollution caused by chemical pesticides, andin addition, is free of conspicuous toxic or harmful effect on seeds, and is environmental-friendly and safe.

The invention belongs to the technical field of biological medicines, and particularly relates to a sinomenine derivative as well as a preparation method and application thereof. Experiments prove that the sinomenine derivative has no obvious toxic effect on cells and is relatively good in safety, and research finds that the sinomenine derivative achieves a remarkable anti-fibrosis effect by reducing the content of alpha-sma, cologen1 and fibronectin in vivo, and the anti-fibrosis effect is remarkably improved compared with sinomenine; even, the safety and the anti-fibrosis effect are better than those of a positive control drug, remarkable progress is achieved, and a brand-new anti-fibrosis drug is provided.

The invention belongs to the technical field of biological medicine, and particularly relates to a redox double-sensitive type nano drug delivery system as well as a preparation method and application thereof. The redox double-sensitive nano drug delivery system provided by the invention comprises a polymer as shown in a general formula (I), p is equal to 5-60, R1, R2, R3 and R4 are independently selected from alkyl or naphthenic base with the number of carbon atoms being less than 6, and x, y, m and n are independently positive integers being less than 6. The polymer is mainly used as a carrier material to promote the formation of a nano drug delivery system, has good biological safety and biocompatibility, and also has good biodegradability and high drug loading capacity. (I).