38results about How to "No obvious toxic effect" patented technology

The invention discloses a preparation method of a zinc oxide-graphene oxide composite nanomaterial, which is implemented through the following steps: by taking soluble divalent zinc ions as a zinc source, taking the soluble divalent zinc ions, graphene oxide and alkali liquor as reactants together, according to a situation that the mass ratio of the zinc ions to the graphene oxide is (1-3): 1, forming a solute composition; by taking alcohol as a solvent, forming a reaction solution mixed system; and after a reaction is performed for 30 min-5 h, purifying a substrate by using centrifugation and washing methods, so that the zinc oxide-graphene oxide composite nanomaterial is obtained. The zinc oxide-graphene oxide composite nanomaterial prepared according to the invention has an effective antibacterial ability at low concentration, and has no obvious toxic action on cells. According to the invention, the operation process is simple, the reaction process has no obvious pollution to the environment, the production cost is low, and the prepared nanomaterial can be applied to the antisepsis of medical apparatuses and instruments, and has a potential in commercial large-scale production.

The invention discloses a rhodamin B double-sulfur fluorescence probe for detecting aqueous mediums and intracellular mercury ions, preparation and application thereof. According to rhodamin B double-sulfur fluorescence probe, molecular closed loops in the rhodamin fluorescence probe are opened in the aqueous mediums by chelating and coordinating the mercury ions, so that fluorescence is generated; the fluorescence intensity is in a good linear relation with the initial concentration of the mercury ions; the color change can be seen by naked eyes when a probe solution is changed from colorless to pink; meanwhile, the fluorescence probe is high in specific selectivity on the mercury ions; in addition, the fluorescence probe is high in biocompatibility and cell permeability and low in cytotoxicity, can be used for detecting the intracellular mercury ions and is high in detection sensitivity of the mercury ions. The preparation of the rhodamin B double-sulfur fluorescence probe is characterized by taking the rhodamin B as the raw material, and enabling the rhodamin B to react with phosphorus oxychloride to prepare a rhodamin B acyl chloride intermediate; the acyl chloride intermediate is further condensed with a double-sulfur intermediate to obtain a mercury ion probe with high sensitivity and high selectivity.

The invention relates to a medicinal preparation for treating benign prostate hyperplasia and process for its preparation, wherein the medicament is prepared from astragalus root, peach kernels, zedoary, rhubarb horsetails, poria cocos, coix seed, motherwort, selfheal, Cinnamomum cassia, root of ballon flower, subprostrate sophora root, achyranthes and cyathula root, and medicinal auxiliary materials.

The invention provides a molecular design and preparation technique of a novel multifunctional targeted nanocapsule anticancer drug. The technique comprises the following steps: wrapping paclitaxel with a carboxylated block copolymer F127-COOH and P123 to form a nano micelle, and forming a silicon-dioxide-shell stable micelle structure in the micelle by using tetramethoxysilane to form drug-carrying nanocapsules; and coupling carboxyl groups on the nanocapsule surface with amino groups on the TRAIL protein surface by using a coupling agent to form the surface-TRAIL-protein-coupled drug-carrying nanocapsules. The technique has the advantages of favorable repetitiveness and mild conditions. The prepared multifunctional targeted nanocapsule anticancer drug has the advantages of high stability, wide anticancer spectrum and obvious curative effects.

The invention discloses a preparation method and application of a curcumin chitosan-stearic acid graft micelle. According to the invention, curcumin is used as a bulk drug, and a graft formed by chitosan oligosaccharide and stearic acid is used as a drug carrier; the preparation method comprises a first step of carrying out covalent binding on chitosan oligosaccharide and stearic acid under the action of the cross-linking agent of carbodiimide so as to form a chitosan oligosaccharide-stearic acid graft (CSO-SA) carrier and a second step of adding a certain amount of a curcumin DMSO solution into a solution of the CSO-SA carrier and carrying out complexation to form a CSO-SA / curcumin drug-loaded nanometer micelle. The micelle prepared in the invention has an average particle size of 114.7 nm and a Zeta potential of 18.5 mV. In-vivo and in- In-vivo tests show that CSO-SA / curcumin has strong cellular internalization capacity, can improve accumulation of curcumin in tumor cells and has a strong killing effect on MCF-7, MCF-7 / Adr and colorectal cancer cells. In-vivo administration enables selective inhibition of growth of tumor cells to be realized while no obvious toxic effect is exerted on mice.

The invention provides a synthetic fiberboard moisture and mildew prevention treatment technology. The technology comprises the following steps: 1, processing sodium silicate crystals Na2O.nSiO2 and sodium fluorosilicate crystals to prepare solutions, uniformly mixing the sodium silicate solution with the sodium fluorosilicate solution according to a mass ratio of 1:999-999:1, disposing the obtained mixed solution for 24h or above, and filing a container with the mixed solution; and 2, coating the surface of a synthetic fiberboard with the mixed solution through adopting a pressure spraying method or an in-container impregnation method to complete moisture and mildew prevention treatment. The technology can prevent influences of mildews induced by moisture absorption of synthetic fiberboards on the environment quality and the property loss, promotes development of the synthetic fiberboard industry, and is in favor of protecting the natural ecological forest. The treatment technology has the advantages of simplicity, low cost, short treatment time, and effective solving of the synthetic fiberboard moisture and mildew prevention problem.

The invention discloses pseudomonas fluorescens as well as a method for preparing a hydroxamic acid type iron carrier by utilizing the pseudomonas fluorescens and an application thereof. According to the invention, the hydroxamic acid type iron carrier is generated by utilizing the culture process of Pseudomonas fluorescens fluorescens strain HMP01, and the hydroxamic acid type iron carrier has a chelating effect on heavy metals, and has a solubilizing effect on organic matters, cationic heavy metals, polycyclic aromatic hydrocarbons, petroleum hydrocarbons and composite pollutants thereof in weakly acidic, neutral and weakly alkaline soil of agricultural lands, industrial and mining areas, oil production areas and the like can be efficiently removed in an ex-situ leaching or in-situ leaching mode, and the hydroxamic acid type iron carrier is simple in preparation process, low in cost, high in leaching efficiency and free of secondary pollution.

A Chinese medicine for treating cough and asthma is prepared from 4 Chinese-medicinal materials including scutellaria root, platycodon root, phabitis seed, etc. Its advantages are high curative effect, no toxic by-effect and low cost.

The invention discloses a preparation method of ganoderan nano-selenium, the ganoderan nano-selenium and application thereof. The preparation method comprises the steps that 1 a sodium selenite solution and a ganoderan solution are mixed to be uniform, ascorbic acid is dropwise added, a constant-temperature stirring reaction is conducted, and a reaction solution is obtained; and (2) the reaction solution obtained in the step (1) is dialyzed to obtain a ganoderan nano-selenium solution. Sodium selenite is used as a selenium source, ascorbic acid is used as a reducing agent, ganoderan is used asa surface modifier, a surface-functionalized nano-selenium compound is prepared in a green mode, and the ganoderan nano-selenium prepared through the preparation process has the advantages of being small in particle size, good in dispersity, amorphous, low in toxicity and good in stability, and can be used as a medicine for preventing and / or treating cancers.

The invention discloses a health-care food composition with a physical fatigue relieving function and a preparation method thereof. A formula of the health-care food composition comprises the following components in parts by weight: 8.7 to 12.3 parts of guarana extract, 32 to 37 parts of taurine, 9.2 to 12.1 parts of glycine, 0.39 to 0.85 part of glutamic acid, 0.1 to 0.3 part of vitamin B1, 0.17 to 0.42 part of vitamin B2 and 1.5 to 3.6 parts of vitamin B6. The preparation method refers to the description. A preparation of the health-care food composition disclosed by the invention can be a beverage, a capsule, a tablet, a chewable tablet, a granular formulation, an oral solution, a soft capsule, paste, flowing liquid and semi-flowing liquid. A health-care product disclosed by the invention takes caffeine, the taurine and total amino acid as functional components; a safety toxicology test, a physical fatigue relieving function test, a hygienic test and a quality stability test show that the health-care food is safe, has no toxin and has a remarkable physical fatigue relieving health-care function.

The invention provides an application of a pharmaceutical composition in the preparation of medicaments for treating obesity, hyperlipidemia or fatty liver, wherein the pharmaceutical composition is prepared by active pharmaceutical ingredients according to the following weight ratios: 100-300 portions of betel nut, 200-600 portions of rhubarb, 200-600 portions of semen pharbitidis, 25-75 portions of fructus gleditsiae abnormalis, 100-300 portions of rhizoma cyperi and 100-200 portions of faeces trogopterori. The pharmaceutical composition has very good treatment or antergic effects to experimental fatty liver caused by high fat diet diseases, can effectively reduce the contents of cholesterol, low density lipoprotein and very low density lipoprotein in serum and increase the content of high density lipoprotein, and has synergistic effect, clear effective substances and controllable quality, without obvious toxic effect, thus being a novel medicament for treating the fatty liver.

The invention belongs to the field of compounds and relates to the use of compound NZ001 in liver cancer metastasis. The present invention proves through in vitro and in vivo experiments that the compound NZ001 (C34H30F2N4O6S) can inhibit the proliferation of highly metastatic liver cancer cells, promote apoptosis and inhibit cycle, inhibit tumor angiogenesis, and inhibit intrapulmonary metastasis and intrahepatic dissemination of tumors. The present invention has been tested, and the results have confirmed the important role of simultaneous inhibition of MET and VEGFR2 signaling pathways in the treatment of liver cancer metastasis, and provide new targets and ideas for clinical treatment of liver cancer metastasis.

A Chinese medicine for preventing and treating hepatic fibrosis is prepared from cordyceps, astragalus root, red sage root, fleece flower root, wolfberry fruit and pseudostellaria root through extracting to obtain liquid, and including the liquid extract by liposome. Its advantages are sure curative effect and high safety.

The invention relates to an application of a fibroblast growth factor. The fibroblast growth factor is a fibroblast growth factor 6, and the application is an application in a medicine for relieving non-alcoholic steatohepatitis liver injury. The application has the following advantages: after acting, the fibroblast growth factor 6 has no obvious toxic effect, and can effectively relieve the NASHliver injury; inflammatory cell aggregation and collagen fiber deposition can be effectively relieved; macrophage activation can be effectively inhibited; and the expression of inflammatory factors inthe liver can be effectively reduced.

The invention discloses a method for evidently increasing Litsea cubeba cluster bud rooting rate. The method includes the steps of firstly, separating Litsea cubeba cluster buds to obtain single buds;secondly using an anti-browning agent perform soaking pretreatment on the base cut of each single bud, wherein the anti-browning agent comprises astaxanthin; thirdly, inoculating the pretreated single buds to a seedling strengthening culture medium to perform seedling strengthening culture; fourthly, transferring the single buds after the seedling strengthening culture to a primary culture mediumto perform primary rooting culture to induce root primordium formation; fifthly, transferring the single buds after the primary rooting culture to a subculture medium to perform rooting subculture toobtain rooted seedlings, wherein the subculture medium comprises Litsea cubeba root tip tissue extract. The method has the advantages that cluster bud death caused by tissue browning during culture can be reduced greatly, the rooting efficiency and root growth speed of the Litsea cubeba cluster buds are increased evidently, Litsea cubeba rapid propagation efficiency is increased, and Litsea cubeba industry development is promoted.

The invention discloses liquid for eliminating microbial pollution and an application method thereof. The liquid contains povidone iodine and PEG 8000. The liquid capable of eliminating the microbialpollution provided by the invention can kill fungi, bacteria, viruses, mycoplasma and other microbial pollution in a broad-spectrum mode and has no obvious toxic effect on cells; and in addition, theliquid is simple to prepare, the preparation cost is low, the popularization is easy, and meanwhile, the problems of microbial pollution caused by biological sample storage, cell culture and the likecan be easily, conveniently and rapidly solved.

The invention discloses application of panax japonicus extract in preparing medicine of preventing osteoporosis. The panax japonicus extract disclosed by the invention is extracted from natural traditional Chinese medicine panax japonicus; the panax japonicus is a common Chinese herbal medicine. According to the long-term traditional Chinese medicine application experience, the panax japonicus is high in using safety and free of remarkable toxic effects. The panax japonicus extract is remarkable in preventing osteoporosis, free of hepatotoxicity and safer than the existing phosphorus tablet. Therefore, the panax japonicus extract has a prospect of being developed into the medicine of preventing osteoporosis.

The invention discloses a functional composition for improving anemia as well as a preparation method and application thereof. The functional composition comprises the following components in parts byweight: 20-50 parts of jujube extract, 20-50 parts of phaseolus calcaratus extract, 10-30 parts of mulberry extract and 10-30 parts of wolfberry extract. The preparation method comprises the following steps: uniformly mixing the components according to a ratio, drying until the moisture content is 0.1-7wt%, sterilizing, grinding, passing through a 80-mesh sieve, adding pharmacologically common excipients or auxiliary components to prepare the dosage form, thereby obtaining the product. The functional composition for improving anemia provided by the invention has the effect of promoting absorption of the body on nutritive materials such as iron and proteins, can well treat anemia, can achieve the aim of controlling treatment after being taken for a long time, does not have any obvious toxic effect and is safe and reliable to eat; the used raw materials are rich in source and readily available, the needed conditions and equipment are simple, the process is simple and controllable, and the functional composition has very important actual popularization value.

The invention discloses application of lycium ruthenicum anthocyanin to preparing a medicine for inducing the apoptosis of two types of cancer cells. A method of the application includes the followingsteps of firstly, culturing four types of cells MCF-7, HepG2, Mac-T and RAW264.7; secondly, continuing to culture the four types of cells with an anthocyanin culture medium and a normal culture medium for 30 hours; thirdly, conducting a cell flow apoptosis experiment and EDU cell proliferation and using a GeminiSEM 300 Zeiss field emission scanning electron microscope and a fluorescent quantitative PCR for the cells in the second step so that it can be proved that the lycium ruthenicum anthocyanin promotes the apoptosis of two types of cancer cells and has no influences on the other two typesof normal cells. The effect of the lycium ruthenicum anthocyanin of regulating and controlling the apoptosis of the liver cancer cells and breast cancer cells is disclosed for the first time. Compared with the prior art, it is proved that the lycium ruthenicum anthocyanin can induce the apoptosis of the human breast cancer cells MCF-7 and the liver cancer cells HepG2 but has no remarkable toxic effect on the cattle mammary gland epithelial cells Mac-T and mouse macrophages RAW264.7, so the lycium ruthenicum anthocyanin can be effectively applied to the research and development of anti-cancerpreparations.

The invention discloses application of panax japonicus extract in preparing medicine of preventing osteoporosis. The panax japonicus extract disclosed by the invention is extracted from natural traditional Chinese medicine panax japonicus; the panax japonicus is a common Chinese herbal medicine. According to the long-term traditional Chinese medicine application experience, the panax japonicus is high in using safety and free of remarkable toxic effects. The panax japonicus extract is remarkable in preventing osteoporosis, free of hepatotoxicity and safer than the existing phosphorus tablet. Therefore, the panax japonicus extract has a prospect of being developed into the medicine of preventing osteoporosis.

The invention relates to application of a Herba Ecliptae extract to preparation of anti-osteoporosis multi-target medicines or healthcare products.The Herba Ecliptae extract is obtained by steps: adding Herba Ecliptae to ethanol, using a Waring blender for blending to a uniform state, performing ethanol extraction, separating and collecting extract liquid, subjecting solid to ethanol extraction again, mixing the extract liquid obtained by two times, performing vacuum concentration to obtain an alcohol extract, adding water into the alcohol extract, and ultrasonically heating to realize dissolution; adding petroleum ether into dissolved solution, and collecting a water-phase part; adding ethyl acetate for extracting the water-phase part to obtain an ethyl acetate part, and performing vacuum concentration and drying to obtain the Herba Ecliptae extract.According to experiments, the Herba Ecliptae extract has an evident inhibition effect on bone resorption of osteoclasts, and in a same dosage range, osteogenic differentiation and mineralization of osteoblast precursors and mesenchymal stem cells are promoted, so that the Herba Ecliptae extract has dual-function multi-target functions in bone resorption inhibition and bone formation promotion and has a prospect of being developed into novel anti-osteoporosis medicines.

The invention belongs to the technical field of active peptides of medicines, and discloses application of Apelin in preparation of medicines for treating silicosis. Tests show that Apelin has a good curative effect on relieving diseases of silicosis, has a good medicinal value prospect, and can be developed into a novel medicine for treating silicosis.

The invention relates to a medicine for treating benign prostatic hyperplasia and a preparation method thereof. Composed of pharmaceutical excipients. The preparation method is to crush cinnamon, rhubarb, coix seed, and peach kernel into fine powder; extract volatile oil from curcuma decoction pieces; Decoction pieces, zedoary dregs, water-extracted filtrate and astragalus ethanol liquid are combined and concentrated to make a dry paste, crushed, sieved, added zedoary volatile oil and fine powder of cinnamon and other drugs, and made into various preparations with medicinal excipients. The preparation can obviously inhibit the hyperplasia of prostate tissue induced by testosterone, significantly reduce the wet weight and dry weight of each leaf of the prostate, has no obvious toxic effect, and is safe and reliable; the preparation method has high bioavailability, good drug effect, stable quality and easy control.

The invention provides a traditional Chinese medicine preparation for external use for treating stroke and a preparation method thereof. The components of the traditional Chinese medicine are reasonable, synergy and interaction of various medicine effects are achieved, and the effects are significant. Qi-tonifying drug and blood-activating drug are matched to make qi vigorous, promote blood circulation and invigorate the blood circulation, and human bodies cannot be affected; phlegm reduction and brain strengthening drug is added and has an assistant effect to reinforce qi and nourish blood, warm and activate meridian, and eliminate phlegm and strengthen brain. The effects of promoting qi circulation, invigorating the blood circulation, eliminating phlegm, promoting blood circulation, andtreating stroke are achieved. According to the traditional Chinese medicine preparation, a hydrogel dosage form for external use is preferred, medicine efficacy can directly treat the illness position, the situations that after oral administration, milder medicine efficacy may cause endogenous heat evil and tepidity and yin impairment can be avoided, the use is convenient, peroral misery is avoided, and the traditional Chinese medicine preparation is particularly applicable to paralytic patients. According to the traditional Chinese medicine preparation, the transdermal absorption is achieved,continuous administration is performed, the transdermal release and adsorption are good, and significant effects of treating stroke symptoms such as facial paralysis, drooling, stiff tongue and speaking difficulty, numb muscles, arthralgia and myalgia and the like which are caused by wind-evil and collateral stroke are achieved.

The invention belongs to the field of agriculture and in particular relates to a seed soaking medicine composition which is prepared from the following raw materials in parts by weight: 20-30 parts oftea leaves, 15-18 parts of a tobacco extract, 1-3 parts of fennel, 1.5-2.5 parts of tea saponin, 1-3 parts of purple sweet potato anthocyanin, 4-6 parts of citrus peel, 10-15 parts of kelp powder, 8-12 parts of acanthopanax roots, 1-5 parts of wormwood, 1-6 parts of tetrapanax papyriferus, 5-8 parts of coal ash, 2-3 parts of a water retaining agent, 1-2 parts of tomato juice, 4-9 parts of cassiabarks and 3-8 parts of sesame oil. Tests on the seed soaking medicine composition provided by the invention show that compared with normal seeds, seeds soaked with the composition are very high in insect expelling performance and germination performance, and the composition is capable of effectively protecting crop seeds, expelling insects and reducing pollution caused by chemical pesticides, andin addition, is free of conspicuous toxic or harmful effect on seeds, and is environmental-friendly and safe.

The invention belongs to the technical field of biological medicines, and particularly relates to a sinomenine derivative as well as a preparation method and application thereof. Experiments prove that the sinomenine derivative has no obvious toxic effect on cells and is relatively good in safety, and research finds that the sinomenine derivative achieves a remarkable anti-fibrosis effect by reducing the content of alpha-sma, cologen1 and fibronectin in vivo, and the anti-fibrosis effect is remarkably improved compared with sinomenine; even, the safety and the anti-fibrosis effect are better than those of a positive control drug, remarkable progress is achieved, and a brand-new anti-fibrosis drug is provided.

The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to a traditional Chinese medicine preparation for treating hypotension as well as a preparation method and an application thereof. The traditional Chinese medicine preparation comprises the following raw materials in parts by weight: 4-8 parts of radix ginseng rubra, 8-16 parts of radixophiopogonis, 4-8 parts of radix bupleuri, 4-8 parts of fructus aurantii immaturus, 4-8 parts of fructus schisandrae chinensis and 4-8 parts of radix glycyrrhizae, and is prepared by extracting according to a certain process and mixing with pharmaceutically acceptable auxiliary materials. Under the guidance of traditional Chinese medicine theories and clinical experience, the curative effect is improved by utilizing the synergistic effect of the medicinal materials, and the traditional Chinese medicine preparation has the effects of relieving dizziness, tiredness and fatigue, shortness of breath and chest distress, palpitation and amnesia, hand and foot unwarmness, thin and white tongue coating, weak pulse and weakness and other symptoms of hypotensive patients mostly caused by qi-yin deficiency and yang-qi deficiency. A therapy of tonifying qi and yin and invigorating splenic yang and restoring pulse is adopted, so that the aim of treatment of hypotension is fulfilled. The traditionalChinese medicine preparation is a pure traditional Chinese medicine formula, is safe and free of toxic and side effects and is particularly suitable for qi-yin deficiency type hypotensive populations.

The invention belongs to the technical field of biological medicine, and particularly relates to a redox double-sensitive type nano drug delivery system as well as a preparation method and application thereof. The redox double-sensitive nano drug delivery system provided by the invention comprises a polymer as shown in a general formula (I), p is equal to 5-60, R1, R2, R3 and R4 are independently selected from alkyl or naphthenic base with the number of carbon atoms being less than 6, and x, y, m and n are independently positive integers being less than 6. The polymer is mainly used as a carrier material to promote the formation of a nano drug delivery system, has good biological safety and biocompatibility, and also has good biodegradability and high drug loading capacity. (I).