39 results about "Sinomenine" patented technology

The invention belongs to the technical field of medicine detection, and particularly discloses a method for detecting related substances in sinomenine hydrochloride raw material medicine and application, and the method comprises the following steps: preparing a test solution; preparing a contrast solution; preparing a mixed impurity reference substance solution; according to the determination method, the five known impurities in the sinomenine hydrochloride raw material can be accurately and quantitatively analyzed, the impurity content of the sinomenine hydrochloride raw material can be more accurately and comprehensively reflected, and the quality of sinomenine hydrochloride can be better controlled.

This invention belongs to the field of traditional Chinese medicine preparation analysis, specifically disclosing a one-test-multiple-evaluation method for determining the content of seven components in Jinteng Qingbi granules and its application. Using sinomenine as an internal standard, a one-test-multiple-evaluation method is used to establish relative correction factors between sinomenine and gallic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C in Jinteng Qingbi granules. The contents of gallic acid, neochlorogenic acid, sinomenine, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C in Jinteng Qingbi granules are calculated using these correction factors. This invention's method is highly practical, simple to operate, cost-effective, and provides accurate and reliable test results, overcoming the shortcomings of existing methods for quality testing of Jinteng Qingbi granules and providing a new approach for quality control and intrinsic quality evaluation of Jinteng Qingbi granules.

The invention discloses a caulis sinomenii root tissue endophytic bacterium strain L2-15 and application thereof, and belongs to the technical field of microorganisms. The bacterial strain is bacillus aryabhattai L2-15, and is preserved in the China Center for Type Culture Collection on February 19, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2025259; the problem of insufficient supply of caulis sinomenii in traditional sinomenine synthesis is solved.

The invention relates to the technical field of new application of sinomenine, and discloses application of sinomenine in preparation of drugs. The invention relates to application of sinomenine in preparation of drugs for inhibiting RA synovial fibroblast proliferation and the like. The sinomenine can significantly inhibit the proliferation of RA-FLS, and plays a role in inducing cell apoptosis at the G0 / G1 phase by retarding the cell cycle. The sinomenine can also be used for remarkably inhibiting the phosphorylation level of downstream effector molecules p70S6K and 4E-BP1 of the mTOR. The sinomenine can also be combined with RNF7 in a targeted manner to inhibit an mTOR signal channel.

The invention belongs to the technical field of traditional Chinese medicine separation and purification, and discloses a method for separating caulis sinomenii alkaloid by a microfiltration-ultrafiltration-nanofiltration-electrodialysis integrated membrane technology. An ultrafiltration membrane electrodialysis device is adopted, the ultrafiltration membrane electrodialysis device comprises an anode plate, a cathode plate and a membrane stack arranged between the anode plate and the cathode plate, and the membrane stack is formed by connecting n groups of electrodialysis units in series, the device sequentially comprises an anode, an anode chamber, [a cation exchange membrane, an acid chamber, an anion exchange membrane, a feeding chamber, an ultrafiltration membrane and a product chamber] n, a cation exchange membrane, a cathode chamber and a cathode from the anode to the cathode. According to the method, the maximum recovery rate of sinomenine hydrochloride can reach 84.1%, the recovery rate of magnoflorine can reach 68.2%, the method is a simple and efficient multi-membrane integrated separation method with low energy consumption and small pollution, and a new thought is provided for separation and purification in the field of traditional Chinese medicine alkaloids.

The invention belongs to the technical field of medicines, and discloses application of a compound M1 shown as a formula (I) and pharmacodynamically acceptable salt thereof in preparation of medicines for preventing, relieving and / or treating autoimmune demyelination diseases. On a mouse experimental autoimmune encephalomyelitis model, the compound M1 shows a good treatment effect, can effectively prevent and treat pathological changes and disease progression of diseases, relieve the severity of the diseases and inhibit LPS-stimulated microglial BV2 cells from releasing inflammatory mediators, and provides a new drug and a new structure for clinical treatment.

The invention discloses a method for separating and purifying Sichuan pepper alkaloid based on macroporous adsorption resin and determining the content of the Sichuan pepper alkaloid. The method comprises the steps of extracting, separating and purifying the Sichuan pepper alkaloid, determining the content and formulating the quality standard of the Sichuan pepper alkaloid. NKA-9 type macroporous adsorption resin is adopted to effectively separate and enrich pepper alkaloid, and resin pretreatment conditions, an extraction process and elution conditions (the ethanol concentration is 40%-95%, the solid-liquid ratio is 1: 10-1: 30, the extraction temperature is 45-75 DEG C, and the pH is 2.5-5.5) are optimized, so that the purity and the recovery rate of alkaloid are remarkably improved. According to the present invention, the quality standard of the Sichuan pepper alkaloid is established by using the HPLC method, and the content determination methods of sinomenine, ethyoxyl chelerythrine, chelerythrine and rutaecarpin are set, such that the stability, the repeatability and the high recovery rate of the Sichuan pepper alkaloid are ensured. Precision, stability and sample recovery rate experiments prove that the method disclosed by the invention has good stability and repeatability and is suitable for industrial extraction and application of the pepper alkaloid.

The invention provides sinomenine in preparation of a medicine for treating chronic pain of rheumatoid arthritis and application of the sinomenine in preparation of the medicine for treating the chronic pain of the rheumatoid arthritis, and aims at solving the problems that an existing medicine for resisting the rheumatoid arthritis is limited in action target spot and poor in treatment effect. The sinomenine takes a histamine receptor H1R on a nervous system dorsal root ganglion M1 type macrophage as the action target spot; the target spot is intervened to regulate and control polarization of macrophages, reduce expression of histamine H1R in M1 type macrophages and inhibit infiltration of the macrophages, so that chronic pain, including crymodynia, mechanical pain, heat pain and the like, of rheumatoid arthritis is effectively relieved; the sinomenine combined action target can improve pain and arthritis symptoms of a model mouse, reduce the level of inflammatory factors and inhibit LPS-induced macrophage migration and H1R expression, and the sinomenine pharmaceutical composition has various dosage forms, has unique action target and mechanism, has a remarkable treatment effect, and can be used for preparing medicines for treating chronic infectious diseases. The compound is expected to become a novel non-opioid anti-rheumatoid arthritis chronic pain medicine.

The invention belongs to the technical field of medicines, and discloses application of a compound M0 as shown in a formula (I) and pharmacodynamically acceptable salts thereof in preparation of medicines for preventing, relieving and / or treating autoimmune demyelination diseases. On a mouse experimental autoimmune encephalomyelitis model, the compound M0 shows a very good treatment effect, can effectively prevent and treat pathological changes and disease progression of diseases, relieve the severity of the diseases and inhibit LPS-stimulated microglial BV2 cells from releasing inflammatory mediators, and provides a new drug and a new structure for clinical treatment. # imgabs0 #

The invention belongs to the field of polymer chemistry and biomedical engineering, and discloses a construction method and application of a biomimetic membrane coated drug-loaded nano system. According to the system disclosed by the invention, small-molecule traditional Chinese medicine (sinomenine) is loaded in mesopores, and homologous cell membranes wrap drug-loaded nanoparticles to obtain a final nano-drug system which can effectively and passively target nasopharyngeal carcinoma cells. The sinomenine can cooperate with photothermal therapy to jointly induce mitochondrial injury, promote cell apoptosis and cause ICD immunoreaction, the sinomenine inhibits PD-L1 expression to relieve immunosuppression of T cells, meanwhile, infiltration of cytotoxic T cells in tumor sites is increased, distant metastasis of nasopharyngeal carcinoma can be inhibited by inhibiting MMPs and PD-L1, and the nasopharyngeal carcinoma can be inhibited by inhibiting MMPs and PD-L1. Therefore, the effects of enhancing nasopharynx cancer immunotherapy and inhibiting metastasis are achieved.

The invention relates to construction of a Qingbai Tongbi capsule fingerprint spectrum and application of the Qingbai Tongbi capsule fingerprint spectrum in drug quality control. The HPLC fingerprint spectrum of the Qingbai Tongbi capsule has 24 characteristic fingerprint chromatographic peaks, wherein the peak 4 is catechin, the peak 10 is sinomenine, the peak 12 is gallic acid, the peak 13 is magnoflorine, the peak 14 is paeoniflorin, the peak 17 is liquiritin, the peak 18 is 1, 2, 3, 4, 6-O-pentagalloylglucose, and the peak 24 is ammonium glycyrrhizinate.

The invention relates to the technical field of intestinal repair compositions, and particularly discloses an intestinal repair anti-inflammatory preparation as well as a preparation method and application thereof. The invention discloses an anti-inflammatory preparation for intestinal repair. The anti-inflammatory preparation is specifically prepared from the following components in concentration: 20-40 mg / L of human umbilical cord mesenchymal stem cells, 3-8 mg / L of fructus psoraleae powder and 0.1-0.6 mg / L of alkaloid, the alkaloid is selected from one or more of sinomenine and arunine. The invention further provides a preparation method and application of the intestinal repair anti-inflammatory preparation. According to the technical scheme provided by the invention, the prepared preparation has an excellent intestinal anti-inflammatory effect.

The invention provides a eutectic compound of rosmarinic acid and sinomenine as well as a preparation method and application of the eutectic compound, and belongs to the technical field of analgesic drugs. The eutectic compound of rosmarinic acid and sinomenine provided by the invention comprises a crystal form A or a crystal form G. Compared with a mixture of rosmarinic acid and sinomenine, the eutectic compound of rosmarinic acid and sinomenine provided by the invention has a better analgesic effect, can be absorbed quickly and completely, and can stay in vivo for a longer time.

The application provides an application of a TRIM44 expression inhibitor in preparation of a cytarabine-resistant leukemia drug, and belongs to the technical field of bioengineering.The application provides the application of the TRIM44 expression inhibitor in preparation of the cytarabine-resistant leukemia drug.In the application, a key gene TRIM44 affecting AML drug resistance is identified by analyzing a single cell transcriptome data set, the expression of the TRIM44 can be lowered by using sinomenine, the function of regulatory T cells (T-reg cells) is inhibited, the sensitivity of AML cells to a chemotherapy drug is enhanced, a good synergistic treatment effect with cytarabine is shown, the cytarabine resistance of leukemia is reversed, and a new target site and a molecular marker are provided for predicting and improving the treatment effect of patients in clinic.

The application belongs to the technical field of pharmaceutical chemistry and organic compound synthesis, and particularly relates to a sinomenine derivative, a preparation method and application thereof. The sinomenine derivative has a structural formula as shown in formula I. The sinomenine derivative is prepared by taking sinomenine and a halogenated reagent as raw materials, obtaining an intermediate I' through a substitution reaction, and then performing a nucleophilic substitution reaction on the intermediate I' and triaryl phosphine halogen gold to obtain the sinomenine derivative shown in formula I. The sinomenine derivative has significant anti-rheumatoid arthritis activity and anti-sepsis activity, low toxic side effects, high safety factor, and good application prospect.

The invention belongs to the field of analysis of traditional Chinese medicine preparations, and particularly discloses a method for detecting the content of multiple components in herba inulae and caulis lonicerae particles for clearing arthralgia. According to the content detection method, the content of 11 components including sinomenine, neochlorogenic acid, magnoflorine, cryptochlorogenic acid, chlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, harpagoside, ligustilide and ammonium glycyrrhizinate in the preparation is determined by adopting an HPLC (High Performance Liquid Chromatography) multi-wavelength switching method. The quality detection method disclosed by the invention is stable, reliable, high in specificity and good in reproducibility, can be used for comprehensively and effectively controlling the quality of the herba inulae arthralgia clearing granules, and is beneficial to stabilizing the product quality and ensuring the safety and effectiveness of clinical medication, so that the requirements of medical treatment and markets are better met.

The present invention discloses a ROS-enhanced liposome and its preparation method and application, which relates to the technical field of biomedical polymer materials and new dosage forms of pharmaceutical preparations. The liposome is composed of egg yolk lecithin, cholesterol and DSPE-mPEG. 2K The liposome is a membrane material prepared by a thin film dispersion extrusion method to encapsulate different drugs; wherein the drug is a ROS-responsive celecoxib dimer prodrug (CTC); or a combination of a ROS-responsive celecoxib dimer prodrug (CTC) and sinomenine hydrochloride (SIN); or a combination of a ROS-responsive celecoxib dimer prodrug (CTC), sinomenine hydrochloride (SIN) and L-butionine-(S,R)-sulfoximine (L-BSO). The liposome not only has the advantages of good stability and long circulation time, but also can target and enrich RA tissues through ELVIS, release celecoxib in a high ROS environment, and then combine with sinomenine hydrochloride to achieve a combined therapeutic effect.

The present application relates to the technical field of traditional Chinese medicine, and specifically discloses a wet itching soothing lotion and a preparation method thereof, comprising a lotion main ingredient and a decoction promoter, wherein the lotion main ingredient is prepared from the following raw materials in parts by weight: 30-50 parts of Kochia scoparia, 26-30 parts of Smilax glabra, 26-34 parts of Lysimachia chinensis, 16-28 parts of Cnidium monnieri, 12-18 parts of Stemona officinalis, 15-21 parts of Sophora flavescens, 28-32 parts of Alumen, 28-40 parts of Dictamni cortex, 12-16 parts of Polygonum multiflorum and 8-10 parts of Phellodendron chinense; the decoction promoter of the present application contains sinomenine, which can not only exert its anti-inflammatory and analgesic effects by itself, but also work together with cocamidopropyl betaine as a surfactant to promote the extraction of active ingredients such as flavonoids and phytosterols in the raw materials. At the same time, the preparation method of the present application is simple, improves the bioavailability of active ingredients of the drug, does not need to be combined with other treatment methods, and improves the healing effect of inflammation caused by perianal eczema.

The invention belongs to the technical field of biological medicine, and particularly relates to an IRF3 small-molecule inhibitor, a preparation method thereof and application of the IRF3 small-molecule inhibitor in inflammatory diseases. Specifically, sinomenine is subjected to structural modification, multiple sinomenine-furyl derivatives are synthesized, a screening system with IFNB1 as a promoter and a macrophage model are combined to verify the inhibitory activity of a small molecule drug Sim-9, and it is further confirmed through a cell thermal displacement analysis technology that the compound Sim-9 is directly targeted to IRF3 protein. And the improvement effect of the compound on inflammatory diseases such as sepsis, acute pancreatitis and the like is verified. Therefore, the method has good practical application value.

The invention belongs to the technical field of biological medicines, and provides a nano-drug for treating renal fibrosis and a preparation method thereof. The preparation method comprises the following steps: dissolving 1, 6-hexamethylene diisocyanate, 2, 2 '-(propane-2, 2-diyl bis (thiodiyl)) bis (ethane-1-ol) and 2, 2-bis (bromomethyl) propane-1, 3-diol in a high-polarity aprotic solvent, and stirring at constant temperature for the first time to obtain a first solution; adding polyethylene glycol 2000 monomethyl ether, and stirring at constant temperature for the second time; dialyzing and drying to obtain a polymer PPh3; dissolving the polymer PPh3 and triphenylphosphine in a high-polarity aprotic solvent to obtain a second solution, stirring at constant temperature for the third time, dialyzing and drying to obtain a polymer PHPDM-PPh3; and the sinomenine and the PHPDM-PPh3 are subjected to nano precipitation and self-assembly to form the nano sinomenine. The nano-drug provided by the invention can improve the targeting property of the drug, is used for treating renal fibrosis, and also can reduce the side effect of sinomenine.

The invention belongs to the technical field of medicines, and discloses synthesis of sinomenine derivatives and application of the sinomenine derivatives in resisting glioma. The invention specifically discloses a sinomenine derivative as shown in a formula I, a synthesis method thereof and an application of the sinomenine derivative in resisting glioma. A new method is provided for structural modification of sinomenine, and part of the compounds can well inhibit proliferation of brain glioma cells and have the potential of serving as anti-glioma drugs. # imgabs0 #

The extraction method of effective components of traditional Chinese medicine composition for treating knee osteoarthritis based on entropy weight method and response surface method belongs to the technical field of traditional Chinese medicine extraction, establishes the content determination method of index components in the composition, i.e., sinomenine, magnoflorine, paeonol glycoside, paeoniflorin and dioscin, and carries out methodology investigation; the comprehensive score of index component content is calculated by the entropy weight method as an evaluation index, the influence factors are ethanol concentration, solid-liquid ratio and soaking time, the experiment is designed by applying the response surface method, the index weight is determined by the comprehensive weighting theory of evaluation index, the comprehensive score is calculated, the multiple quadratic regression model of comprehensive score and investigation factors is established, the variance analysis is carried out, the optimal process is predicted and verified, and the optimized extraction process parameters of Jiantongkang are determined. The extraction condition optimization research of index components of the composition based on the entropy weight method and the response surface method is strong in pertinence, simple in operation, high in accuracy, scientific and reasonable in process, and is suitable for the content determination of medicinal material effective components.

The invention relates to the technical field of water-soluble fertilizers, and particularly discloses a water-soluble fertilizer for promoting growth and preventing diseases of small watermelons and a preparation method of the water-soluble fertilizer. Comprising the following raw materials in parts by weight: urea, ammonium nitrate, monoammonium phosphate, diammonium phosphate, potassium nitrate, potassium sulfate, calcium chloride, magnesium sulfate, chelated iron, humic acid, amino acid, sugar alcohol calcium, calcium ammonium nitrate, fulvic acid, algal polysaccharides, mannitol, chitin, zhongshengmycin, kasugamycin, ethylicin, a compound microbial agent, photosynthetic bacteria, a wetting agent and a filler. The water-soluble fertilizer is prepared from nitrogen, phosphorus and potassium elements, organic substances and microbial strains and can be applied to the small watermelons in a composite mode, the small watermelons can fully absorb nutrient substances through the action of multiple substances, the purpose of promoting growth of the small watermelons is achieved, and the yield of the small watermelons is increased. And bacterial diseases and fungal diseases of the watermelons can be effectively prevented through microbial strains.

The application provides a composite antibacterial biological preparation for preventing and treating tobacco brown spot, and a preparation method and application thereof, and belongs to the technical field of tobacco disease prevention and treatment.The composite antibacterial biological preparation comprises the following components in parts by weight: Chinese herbal medicine fermentation liquor 50-110 parts, active ingredient 8-25 parts, and auxiliary agent 1.5-3.8 parts; the active ingredient is a mixture of 5-amino levulinic acid hydrochloride and sinomenine and limonene at a mass ratio of 5-10:0.01-0.03:0.15-0.78.The application realizes effective prevention and treatment of tobacco brown spot through synergistic effect of multiple components, and the components are natural, easy to degrade, strong in rainwater erosion resistance, free of pesticide residues and environmentally friendly.

The application discloses a sinomenine derivative, a synthetic method and application thereof, and belongs to the technical field of biology and medicine. In the synthesis, sinomenine and acryloyl chloride are subjected to a room temperature reaction under the catalysis of triethylamine, and then the product in the reaction solution is separated and purified to obtain an intermediate; the intermediate and substituted aniline are subjected to a room temperature reaction under the catalysis of an organic base for 11-13 hours, and then the product in the reaction solution is separated and purified to obtain the sinomenine derivative, which can be used as an antifungal agent or for preparing an antifungal medicine and used in combination with fluconazole as an antifungal medicine synergist. The sinomenine derivative not only provides a new candidate medicine for fungal treatment, but also improves the antifungal effect of the existing medicine.

This invention belongs to the field of pharmaceutical technology and discloses sinomenine derivatives and their application in the treatment of glioma. Specifically, it discloses a sinomenine derivative represented by Formula I, its synthesis method, and its application in the treatment of glioma. This invention provides a new method for the structural modification of sinomenine, and some compounds can effectively inhibit the proliferation of glioma cells, showing potential as anti-glioma drugs.

The invention relates to the technical field of biology, in particular to a cell culture device and a system for evaluating the curative effect of sinomenine on arthritis. In a cell culture device, a culture container is attached to the bottom of a culture chamber. The rotary drum is installed on the upper half portion of the culture chamber, and an annular flange is arranged in the rotary drum and provided with a protruding portion. The air guide pipe is arranged in the rotary drum, and the matching column is in sliding fit with the air guide pipe and is of a hollow structure. A first opening is formed in the end, close to the air guide pipe, of the matching column and matched with a first valve. A second opening is formed in one end of the matching column away from the gas-guide tube. A pushing piece is installed on the outer side wall of the matching column and abuts against the side, away from the bottom of the culture chamber, of the annular flange. The temperature control precision and the temperature stability can be effectively improved, temperature fluctuation can be found in time, it is ensured that cells grow under the preset environment condition, and deviation of the cell growth condition is avoided.

The invention belongs to the technical field of traditional Chinese medicine active component extraction, and relates to an extraction process of traditional Chinese medicine active components, in particular to a microwave extraction process of sinomenine based on a deep-eutectic solvent. The process comprises the following steps: S1, drying a caulis sinomenii medicinal material, crushing, and sieving with a 40-mesh sieve to obtain caulis sinomenii powder; s2, a deep eutectic solvent is prepared, the deep eutectic solvent is formed by mixing choline chloride, water and one or more of glucose, lactic acid and glycerin, and the mass fraction of water in the deep eutectic solvent is 30%-60%; s3, adding the caulis sinomenii powder into a deep eutectic solvent according to a solid-liquid ratio of 1: 20g / mL, and performing microwave extraction for 12-21 minutes under the power of 400-500w to obtain sinomenine. The process adopts microwave-assisted DESs to extract sinomenine, so that the sinomenine yield is increased, and the process is environment-friendly.

The invention discloses a traditional Chinese medicine composition and application, and relates to the technical field of traditional Chinese medicine, the traditional Chinese medicine composition comprises, by weight, 1 part of sinomenine and 0.5-1.5 parts of formononetin, and the traditional Chinese medicine composition is used for treating chicken mycoplasma synoviae. As an innovative therapy for treating chicken mycoplasma synoviae, the traditional Chinese medicine composition provides an efficient and resistance-free treatment choice for farmers due to the characteristics of simple formula and easiness in obtaining. The traditional Chinese medicine composition is only composed of two traditional Chinese medicines, the cost is reduced, the preparation process is simplified, and large-scale application becomes possible. More importantly, the composition avoids negative effects caused by use of antibiotics and is especially suitable for the situation that antibiotics cannot be abused in laying hen breeding, a breeder can effectively control diseases and reduce economic losses under the situation that food safety regulations are not violated, the safety of poultry products is guaranteed, and the requirements of consumers for antibiotic-free products are met.

The invention aims to provide a refining process of a sinomenine hydrochloride bulk drug for injection, which comprises the following steps: taking 130-200 parts by volume of ethanol into a container, heating to reflux, adding the ethanol into a container containing 10 parts by weight of sinomenine hydrochloride, stirring, filtering while hot, cooling filtrate at 0-8 DEG C for crystallization, taking out after overnight, carrying out suction filtration, leaching filter residue with iced ethanol, and drying to obtain the sinomenine hydrochloride bulk drug for injection. And drying at 40 DEG C to obtain refined sinomenine hydrochloride. The refined sinomenine hydrochloride provided by the invention meets the impurity limit requirements of impurities (Q3A) in ICH new bulk drugs.