21 results about "Sinomenine" patented technology

The invention belongs to the technical field of medicine detection, and particularly discloses a method for detecting related substances in sinomenine hydrochloride raw material medicine and application, and the method comprises the following steps: preparing a test solution; preparing a contrast solution; preparing a mixed impurity reference substance solution; according to the determination method, the five known impurities in the sinomenine hydrochloride raw material can be accurately and quantitatively analyzed, the impurity content of the sinomenine hydrochloride raw material can be more accurately and comprehensively reflected, and the quality of sinomenine hydrochloride can be better controlled.

This invention belongs to the field of traditional Chinese medicine preparation analysis, specifically disclosing a one-test-multiple-evaluation method for determining the content of seven components in Jinteng Qingbi granules and its application. Using sinomenine as an internal standard, a one-test-multiple-evaluation method is used to establish relative correction factors between sinomenine and gallic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C in Jinteng Qingbi granules. The contents of gallic acid, neochlorogenic acid, sinomenine, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, and isochlorogenic acid C in Jinteng Qingbi granules are calculated using these correction factors. This invention's method is highly practical, simple to operate, cost-effective, and provides accurate and reliable test results, overcoming the shortcomings of existing methods for quality testing of Jinteng Qingbi granules and providing a new approach for quality control and intrinsic quality evaluation of Jinteng Qingbi granules.

The invention belongs to the technical field of traditional Chinese medicine separation and purification, and discloses a method for separating caulis sinomenii alkaloid by a microfiltration-ultrafiltration-nanofiltration-electrodialysis integrated membrane technology. An ultrafiltration membrane electrodialysis device is adopted, the ultrafiltration membrane electrodialysis device comprises an anode plate, a cathode plate and a membrane stack arranged between the anode plate and the cathode plate, and the membrane stack is formed by connecting n groups of electrodialysis units in series, the device sequentially comprises an anode, an anode chamber, [a cation exchange membrane, an acid chamber, an anion exchange membrane, a feeding chamber, an ultrafiltration membrane and a product chamber] n, a cation exchange membrane, a cathode chamber and a cathode from the anode to the cathode. According to the method, the maximum recovery rate of sinomenine hydrochloride can reach 84.1%, the recovery rate of magnoflorine can reach 68.2%, the method is a simple and efficient multi-membrane integrated separation method with low energy consumption and small pollution, and a new thought is provided for separation and purification in the field of traditional Chinese medicine alkaloids.

The invention belongs to the technical field of medicines, and discloses application of a compound M1 shown as a formula (I) and pharmacodynamically acceptable salt thereof in preparation of medicines for preventing, relieving and / or treating autoimmune demyelination diseases. On a mouse experimental autoimmune encephalomyelitis model, the compound M1 shows a good treatment effect, can effectively prevent and treat pathological changes and disease progression of diseases, relieve the severity of the diseases and inhibit LPS-stimulated microglial BV2 cells from releasing inflammatory mediators, and provides a new drug and a new structure for clinical treatment.

The invention discloses a method for separating and purifying Sichuan pepper alkaloid based on macroporous adsorption resin and determining the content of the Sichuan pepper alkaloid. The method comprises the steps of extracting, separating and purifying the Sichuan pepper alkaloid, determining the content and formulating the quality standard of the Sichuan pepper alkaloid. NKA-9 type macroporous adsorption resin is adopted to effectively separate and enrich pepper alkaloid, and resin pretreatment conditions, an extraction process and elution conditions (the ethanol concentration is 40%-95%, the solid-liquid ratio is 1: 10-1: 30, the extraction temperature is 45-75 DEG C, and the pH is 2.5-5.5) are optimized, so that the purity and the recovery rate of alkaloid are remarkably improved. According to the present invention, the quality standard of the Sichuan pepper alkaloid is established by using the HPLC method, and the content determination methods of sinomenine, ethyoxyl chelerythrine, chelerythrine and rutaecarpin are set, such that the stability, the repeatability and the high recovery rate of the Sichuan pepper alkaloid are ensured. Precision, stability and sample recovery rate experiments prove that the method disclosed by the invention has good stability and repeatability and is suitable for industrial extraction and application of the pepper alkaloid.

The invention provides sinomenine in preparation of a medicine for treating chronic pain of rheumatoid arthritis and application of the sinomenine in preparation of the medicine for treating the chronic pain of the rheumatoid arthritis, and aims at solving the problems that an existing medicine for resisting the rheumatoid arthritis is limited in action target spot and poor in treatment effect. The sinomenine takes a histamine receptor H1R on a nervous system dorsal root ganglion M1 type macrophage as the action target spot; the target spot is intervened to regulate and control polarization of macrophages, reduce expression of histamine H1R in M1 type macrophages and inhibit infiltration of the macrophages, so that chronic pain, including crymodynia, mechanical pain, heat pain and the like, of rheumatoid arthritis is effectively relieved; the sinomenine combined action target can improve pain and arthritis symptoms of a model mouse, reduce the level of inflammatory factors and inhibit LPS-induced macrophage migration and H1R expression, and the sinomenine pharmaceutical composition has various dosage forms, has unique action target and mechanism, has a remarkable treatment effect, and can be used for preparing medicines for treating chronic infectious diseases. The compound is expected to become a novel non-opioid anti-rheumatoid arthritis chronic pain medicine.

The invention belongs to the field of polymer chemistry and biomedical engineering, and discloses a construction method and application of a biomimetic membrane coated drug-loaded nano system. According to the system disclosed by the invention, small-molecule traditional Chinese medicine (sinomenine) is loaded in mesopores, and homologous cell membranes wrap drug-loaded nanoparticles to obtain a final nano-drug system which can effectively and passively target nasopharyngeal carcinoma cells. The sinomenine can cooperate with photothermal therapy to jointly induce mitochondrial injury, promote cell apoptosis and cause ICD immunoreaction, the sinomenine inhibits PD-L1 expression to relieve immunosuppression of T cells, meanwhile, infiltration of cytotoxic T cells in tumor sites is increased, distant metastasis of nasopharyngeal carcinoma can be inhibited by inhibiting MMPs and PD-L1, and the nasopharyngeal carcinoma can be inhibited by inhibiting MMPs and PD-L1. Therefore, the effects of enhancing nasopharynx cancer immunotherapy and inhibiting metastasis are achieved.

The invention relates to the technical field of intestinal repair compositions, and particularly discloses an intestinal repair anti-inflammatory preparation as well as a preparation method and application thereof. The invention discloses an anti-inflammatory preparation for intestinal repair. The anti-inflammatory preparation is specifically prepared from the following components in concentration: 20-40 mg / L of human umbilical cord mesenchymal stem cells, 3-8 mg / L of fructus psoraleae powder and 0.1-0.6 mg / L of alkaloid, the alkaloid is selected from one or more of sinomenine and arunine. The invention further provides a preparation method and application of the intestinal repair anti-inflammatory preparation. According to the technical scheme provided by the invention, the prepared preparation has an excellent intestinal anti-inflammatory effect.

The invention provides a eutectic compound of rosmarinic acid and sinomenine as well as a preparation method and application of the eutectic compound, and belongs to the technical field of analgesic drugs. The eutectic compound of rosmarinic acid and sinomenine provided by the invention comprises a crystal form A or a crystal form G. Compared with a mixture of rosmarinic acid and sinomenine, the eutectic compound of rosmarinic acid and sinomenine provided by the invention has a better analgesic effect, can be absorbed quickly and completely, and can stay in vivo for a longer time.

The application provides an application of a TRIM44 expression inhibitor in preparation of a cytarabine-resistant leukemia drug, and belongs to the technical field of bioengineering.The application provides the application of the TRIM44 expression inhibitor in preparation of the cytarabine-resistant leukemia drug.In the application, a key gene TRIM44 affecting AML drug resistance is identified by analyzing a single cell transcriptome data set, the expression of the TRIM44 can be lowered by using sinomenine, the function of regulatory T cells (T-reg cells) is inhibited, the sensitivity of AML cells to a chemotherapy drug is enhanced, a good synergistic treatment effect with cytarabine is shown, the cytarabine resistance of leukemia is reversed, and a new target site and a molecular marker are provided for predicting and improving the treatment effect of patients in clinic.

The application belongs to the technical field of pharmaceutical chemistry and organic compound synthesis, and particularly relates to a sinomenine derivative, a preparation method and application thereof. The sinomenine derivative has a structural formula as shown in formula I. The sinomenine derivative is prepared by taking sinomenine and a halogenated reagent as raw materials, obtaining an intermediate I' through a substitution reaction, and then performing a nucleophilic substitution reaction on the intermediate I' and triaryl phosphine halogen gold to obtain the sinomenine derivative shown in formula I. The sinomenine derivative has significant anti-rheumatoid arthritis activity and anti-sepsis activity, low toxic side effects, high safety factor, and good application prospect.

The invention belongs to the technical field of biological medicines, and provides a nano-drug for treating renal fibrosis and a preparation method thereof. The preparation method comprises the following steps: dissolving 1, 6-hexamethylene diisocyanate, 2, 2 '-(propane-2, 2-diyl bis (thiodiyl)) bis (ethane-1-ol) and 2, 2-bis (bromomethyl) propane-1, 3-diol in a high-polarity aprotic solvent, and stirring at constant temperature for the first time to obtain a first solution; adding polyethylene glycol 2000 monomethyl ether, and stirring at constant temperature for the second time; dialyzing and drying to obtain a polymer PPh3; dissolving the polymer PPh3 and triphenylphosphine in a high-polarity aprotic solvent to obtain a second solution, stirring at constant temperature for the third time, dialyzing and drying to obtain a polymer PHPDM-PPh3; and the sinomenine and the PHPDM-PPh3 are subjected to nano precipitation and self-assembly to form the nano sinomenine. The nano-drug provided by the invention can improve the targeting property of the drug, is used for treating renal fibrosis, and also can reduce the side effect of sinomenine.

The extraction method of effective components of traditional Chinese medicine composition for treating knee osteoarthritis based on entropy weight method and response surface method belongs to the technical field of traditional Chinese medicine extraction, establishes the content determination method of index components in the composition, i.e., sinomenine, magnoflorine, paeonol glycoside, paeoniflorin and dioscin, and carries out methodology investigation; the comprehensive score of index component content is calculated by the entropy weight method as an evaluation index, the influence factors are ethanol concentration, solid-liquid ratio and soaking time, the experiment is designed by applying the response surface method, the index weight is determined by the comprehensive weighting theory of evaluation index, the comprehensive score is calculated, the multiple quadratic regression model of comprehensive score and investigation factors is established, the variance analysis is carried out, the optimal process is predicted and verified, and the optimized extraction process parameters of Jiantongkang are determined. The extraction condition optimization research of index components of the composition based on the entropy weight method and the response surface method is strong in pertinence, simple in operation, high in accuracy, scientific and reasonable in process, and is suitable for the content determination of medicinal material effective components.

The application provides a composite antibacterial biological preparation for preventing and treating tobacco brown spot, and a preparation method and application thereof, and belongs to the technical field of tobacco disease prevention and treatment.The composite antibacterial biological preparation comprises the following components in parts by weight: Chinese herbal medicine fermentation liquor 50-110 parts, active ingredient 8-25 parts, and auxiliary agent 1.5-3.8 parts; the active ingredient is a mixture of 5-amino levulinic acid hydrochloride and sinomenine and limonene at a mass ratio of 5-10:0.01-0.03:0.15-0.78.The application realizes effective prevention and treatment of tobacco brown spot through synergistic effect of multiple components, and the components are natural, easy to degrade, strong in rainwater erosion resistance, free of pesticide residues and environmentally friendly.

The application discloses a sinomenine derivative, a synthetic method and application thereof, and belongs to the technical field of biology and medicine. In the synthesis, sinomenine and acryloyl chloride are subjected to a room temperature reaction under the catalysis of triethylamine, and then the product in the reaction solution is separated and purified to obtain an intermediate; the intermediate and substituted aniline are subjected to a room temperature reaction under the catalysis of an organic base for 11-13 hours, and then the product in the reaction solution is separated and purified to obtain the sinomenine derivative, which can be used as an antifungal agent or for preparing an antifungal medicine and used in combination with fluconazole as an antifungal medicine synergist. The sinomenine derivative not only provides a new candidate medicine for fungal treatment, but also improves the antifungal effect of the existing medicine.

This invention belongs to the field of pharmaceutical technology and discloses sinomenine derivatives and their application in the treatment of glioma. Specifically, it discloses a sinomenine derivative represented by Formula I, its synthesis method, and its application in the treatment of glioma. This invention provides a new method for the structural modification of sinomenine, and some compounds can effectively inhibit the proliferation of glioma cells, showing potential as anti-glioma drugs.

The invention relates to the technical field of biology, in particular to a cell culture device and a system for evaluating the curative effect of sinomenine on arthritis. In a cell culture device, a culture container is attached to the bottom of a culture chamber. The rotary drum is installed on the upper half portion of the culture chamber, and an annular flange is arranged in the rotary drum and provided with a protruding portion. The air guide pipe is arranged in the rotary drum, and the matching column is in sliding fit with the air guide pipe and is of a hollow structure. A first opening is formed in the end, close to the air guide pipe, of the matching column and matched with a first valve. A second opening is formed in one end of the matching column away from the gas-guide tube. A pushing piece is installed on the outer side wall of the matching column and abuts against the side, away from the bottom of the culture chamber, of the annular flange. The temperature control precision and the temperature stability can be effectively improved, temperature fluctuation can be found in time, it is ensured that cells grow under the preset environment condition, and deviation of the cell growth condition is avoided.

The invention discloses a traditional Chinese medicine composition and application, and relates to the technical field of traditional Chinese medicine, the traditional Chinese medicine composition comprises, by weight, 1 part of sinomenine and 0.5-1.5 parts of formononetin, and the traditional Chinese medicine composition is used for treating chicken mycoplasma synoviae. As an innovative therapy for treating chicken mycoplasma synoviae, the traditional Chinese medicine composition provides an efficient and resistance-free treatment choice for farmers due to the characteristics of simple formula and easiness in obtaining. The traditional Chinese medicine composition is only composed of two traditional Chinese medicines, the cost is reduced, the preparation process is simplified, and large-scale application becomes possible. More importantly, the composition avoids negative effects caused by use of antibiotics and is especially suitable for the situation that antibiotics cannot be abused in laying hen breeding, a breeder can effectively control diseases and reduce economic losses under the situation that food safety regulations are not violated, the safety of poultry products is guaranteed, and the requirements of consumers for antibiotic-free products are met.

The invention discloses sinomenine hydrochloride solid lipid nanoparticles, gel, a preparation method and application, by exploring the preparation method of the sinomenine hydrochloride solid lipid nanoparticles HA-SH-SLNs, when the mass ratio of sinomenine hydrochloride to soya bean lecithin to hyaluronic acid modified targeting molecule DSPE-PEG-HA to poloxamer Pluronic F-68 is (0.3-0.7): (1-4): (0.0125-0.1): (1-4), the gel can be used for preparing the sinomenine hydrochloride solid lipid nanoparticles HA-SH-SLNs. The sinomenine hydrochloride solid lipid nanoparticles which are uniform in particle size, high in encapsulation efficiency, high in drug loading capacity and stable in quality are obtained, and the preparation method of the sinomenine hydrochloride solid lipid nanoparticle gel is further researched. The obtained sinomenine hydrochloride solid lipid nanoparticle gel HA-SH-SLNs-Gel is good in stability and small in hemolysis rate, the in-vivo residence time is longer than that of sinomenine hydrochloride solid lipid nanoparticles, the drug release period is delayed, and better articular cavity injection administration is facilitated.

This invention belongs to the field of pharmaceutical technology and discloses sinomenine derivatives and their application in the treatment of glioma. Specifically, it discloses a sinomenine derivative represented by Formula I, its synthesis method, and its application in the treatment of glioma. This invention provides a new method for the structural modification of sinomenine, and some compounds can effectively inhibit the proliferation of glioma cells, showing potential as anti-glioma drugs.

The application discloses a quality detection method of Zhuang medicine Luojun Yuba Plaster, which comprises the following steps: adopting UPLC to determine the contents of main components in the Zhuang medicine Luojun Yuba Plaster, i.e.sinomenine, mao liangmianjiesu, fufenji, lüguansu, hujangju and longxuesu B; and adopting thin layer chromatography (TLC) to identify sinofengteng, fufenji, hujang, feilongzhangxue, longxuiezhi, rushan and meiyao in the Zhuang medicine Luojun Yuba Plaster. The quality detection method can accurately and quickly determine the contents of the main components in the Zhuang medicine Luojun Yuba Plaster and effectively identify the used medicinal materials. The quality detection method is simple and high in accuracy, can systematically and accurately evaluate the quality of the Zhuang medicine Luojun Yuba Plaster, is favorable for comprehensively controlling the overall quality of the Zhuang medicine Luojun Yuba Plaster, ensures curative effect, and provides an effective reference for quality control and evaluation of the Zhuang medicine Luojun Yuba Plaster.